Relationships of survival time, productivity and cause of death with telomere lengths of cows produced by somatic cell nuclear transfer

The reproductive ability, milk-producing capacity, survival time and relationships of these parameters with telomere length were investigated in 4 groups of cows produced by somatic cell nuclear transfer (SCNT). Each group was produced using the same donor cells (6 Holstein (1H), 3 Holstein (2H), 4 Jersey (1J) and 5 Japanese Black (1B) cows). As controls, 47 Holstein cows produced by artificial insemination were used. The SCNT cows were artificially inseminated, and multiple deliveries were performed after successive rounds of breeding and conception. No correlation was observed between the telomere length and survival time in the SCNT cows. Causes of death of SCNT cows included accidents, accident-associated infections, inappropriate management, acute mastitis and hypocalcemia. The lifetime productivity of SCNT cows was superior to those of the controls and cell donor cows. All SCNT beef cows with a relatively light burden of lactation remained alive and showed significantly prolonged survival time compared with the cows in the SCNT dairy breeds. These results suggest that the lifetime productivity of SCNT cows was favorable, and their survival time was more strongly influenced by environmental burdens, such as pregnancy, delivery, lactation and feeding management, than by the telomere length.     

Cutaneous lymphoplasmacytic lymphoma with systemic metastasis in a cat

A lymphoplasmacytic lymphoma was diagnosed in a 12- year-old domestic cat that had a primary cutaneous mass involving the stomach, liver, kidneys, heart, abdominal wall, diaphragm, bone marrow and several lymph nodes. Histopathologically, the most characteristic feature of this tumor was the heterogeneity of cell components, such as small lymphocytes, well-differentiated plasma cells and plasmacytoid transformed lymphocytes. Amyloid was deposited in the skin, stomach, and several lymph nodes. Immunohistochemically, neoplastic small lymphocytes were positive for CD20, and well-differentiated plasma cells and plasmacytoid transformed lymphocytes were positive for λ-Ig light chains and MUM1/IRF-4. These results emphasize the importance of lymphoplasmacytic lymphoma as a differential diagnosis of extramedullary cutaneous plasmacytoma in cats.     

Mycobacterium ulcerans infection in an Indian flap-shelled turtle (Lissemys punctata punctata)

We report an atypical mycobacterial infection in an Indian flap-shelled turtle, Lissemys punctata punctata, that died in an aquarium in Japan. At necropsy, the turtle showed multiple white nodules on the capsular surface and parenchyma of various organs such as the liver, spleen, intestine, and lung. Histologically, granulomatous inflammation surrounding a central zone of necrosis was observed. Sections stained by the Ziehl-Neelsen method revealed numerous acid-fast bacilli in the cytoplasm of macrophages and in the central area of necrosis. The organisms were identified as a mycobacterial species by PCR and nucleotide sequence analysis and revealed 98-100% homology to M. ulcerans. This is, to our knowledge, the first report of mycobacteriosis due to M. ulcerans in a turtle.     

Cardiopulmonary function values before and after heartworm removal in dogs with caval syndrome

Cardiopulmonary function values were determined before and after surgical removal of adult heartworms in 25 dogs with spontaneous and 4 dogs with drug-induced caval syndrome (CS). Fifteen dogs with spontaneous CS (recovery group) and 4 dogs with drug-induced CS (drug-induced CS group) recovered after removal, and 10 dogs with spontaneous CS were euthanatized or died (nonsurviving group). Before heartworm removal, injected radiographic contrast medium was regurgitated from the right ventricle to the right atrium. Mean pulmonary arterial pressure and total pulmonary resistance were not statistically different between the recovery and nonsurviving groups of dogs, but the end-diastolic right ventricular pressure (mean +/- SD, 6.9 +/- 9.1 mm of Hg) and the a (8.7 +/- 9.2 mm of Hg)- and v (6.3 +/- 8.5 mm of Hg)-waves of the right atrial pressure curve in the recovery group were less, respectively, than the end-diastolic right ventricular pressure (17.3 +/- 6.0 mm of Hg) and the a (15.8 +/- 6.1 mm of Hg)- and v (21.4 +/- 6.9 mm of Hg)-waves in dogs of the nonsurviving group. After heartworm removal, contrast medium regurgitation disappeared, and cardiac output of the right ventricle increased in dogs of the recovery (from 2.08 +/- 0.72 to 2.38 +/- 0.68 L/min; P less than 0.05) and drug-induced CS (from 1.42 +/- 0.19 to 1.88 +/- 0.26 L/min, P less than 0.05) groups. However, regurgitation remained, and cardiac output did not increase in some dogs of the nonsurviving group.(ABSTRACT TRUNCATED AT 250 WORDS)     

Does paternal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) affect the sex ratio of offspring?

In 1976, men who were exposed to the highest concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) after an explosion at a chemical plant near Seveso, Italy, produced more girls than boys. However, few studies have examined the possibility that the exposure of laboratory animals to TCDD, especially that of males, could lead to a lower male/female sex ratio. The aim of this study was to investigate whether direct paternal exposure to TCDD affects the sex ratio of offspring using a relatively large-scale experimental design. Male ICR mice (n=120) were randomly assigned to three, one of which served as a vehicle control, the other two were administered TCDD orally with an initial loading dose of 2 or 2,000 ng TCDD/kg, followed by a weekly maintenance dose of 0.4 (T2/0.4 group) or 400 (T2000/400 group) ng/kg prior to mating. The major organs of each mouse were weighed and histopathologically and immunohistologically investigated, and the sex ratio of offspring [males/(males + females) x 100] was calculated in each dam. There were no significant effects on organ weights, or on the structure of the testis and epididymis between the control and TCDD-exposed males, but TCDD administration produced a significantly lower proportion of male offspring from T2000/400-exposed sires despite no alteration in litter size (Control: 53.1 +/- 1.7; T2/0.4: 48.8 +/- 2.5; T2000/400: 46.2 +/- 2.1). In addition, we further divided the T2000/400 group into 3 subgroups based on the proportion of CYP1A1-immunoreactive areas in the liver; there was a significant correlation between sex ratio and CYP1A1 immunoreactivity. Thus, the present study confirms that direct paternal exposure to TCDD might be associated with an alteration in the sex ratio of offspring. Possible mechanisms through which TCDD might decrease the fertility potential of Y-bearing gametes before conception are discussed.     

Arrhythmogenic right ventricular cardiomyopathy coincided with the cardiac fibrosis in the inner muscle layer of the left ventricular wall in a boxer dog

A 7-year-old female boxer dog died suddenly without any clinical signs. It was suspected that the dog had arrhythmogenic right ventricular cardiomyopathy (ARVC) due to ventricular premature complexes and ventricular tachycardia at 3 years of age. The final diagnosis of ARVC was confirmed by histological characteristics, such as loss of cardiocytes and fibrofatty replacement, occurring in the right and left ventricular walls. In the cardiocytes, non-lipid vacuoles were observed. Cardiac fibrosis and intimal thickening of the small arteries occurred without fatty replacement in the inner muscle layer including the papillary muscles of the left ventricular wall. This paper describes the pathomorphological details of an ARVC case with coincidental cardiac fibrosis in the inner muscle layer of the left ventricular wall.     

Effect of nitrogen fertilization on methane flux in a structured clay soil cultivated with onion in Central Hokkaido,Japan

Abstract

This study was conducted to investigate the effect of N fertilization on the soil CH₄ flux during the growing season of onion in a structured clay soil with stagnant water at depths of 70–80 cm and with a peat-mixed subsoil. The following 4 treatments were analyzed over a period of two years: T1) fertilized, onion growing, T2) fertilized, bare field, T3) unfertilized, onion growing, and T4) unfertilized, bare field. In the fertilized T1 and T2 treatments, fertilizers (mixture of 3 : 1 NH₄NO₃ : (NH₄)₂SO₄) at rates of 322 kg N ha^?1 in 1999 and 242 kg N ha^?1 in 2000 were applied as basal fertilizers before onion was transplanted. CH₄ fluxes among the treatments ranged from ?0.06 to 0.12 mg CH₄-C m^?2 h^?1 in 1999, and from ?0.03 to 0.01 mg CH₄-C m^?2 h^?1 in 2000, which were high after heavy rain in summer. Cumulative CH₄ flux from May to November in the fertilized T1 and T2 treatments was 59 mg CH₄-C m^?2 for both treatments in 1999, and 3.2 and ?0.9 mg CH₄-C m^?2 in 2000, respectively. On the other hand, in the unfertilized T3 and T4 treatments, the cumulative CH₄ flux was 0.2 and ?9.2 mg CH₄-C m^?2 in 1999, and ?26 and ?20 mg CH₄-C m^?2 in 2000, respectively. Although the cumulative CH₄ flux in each treatment was higher in 1999 than in 2000, the fertilized treatments in both years showed a significantly higher cumulative CH₄ flux than the unfertilized treatments. This might be ascribed to the higher level of nitrification in the fertilized treatments, because a high nitrate concentration was observed in the fertilized treatments in the onion growing season. The results also revealed that onion growing did not exert a significant influence on the CH₄ flux. The precipitation from May to November was 642 mm in 1999 and 1,008 mm in 2000, and the CH₄ emission increased when the precipitation was low. In addition, the CH₄ concentration in the soil profile increased with the increase of the depth in summer as the soil was dry. These findings indicated that CH₄ diffusion from the soil to the atmosphere was inhibited by rainwater.     

Effects of shifting cultivation on soil ecosystems in Sarawak,Malaysia

Experimental shifting cultivation was conducted at the Balai Ringin (B. Ringin) and Sabal sites in Sarawak, Malaysia. At the sites, plots (10 x 10 m²) were burned with the fuel of aboveground biomass amounting to 0 (control), 100, 200, and 300 Mg ha^-1. At the B. Ringin site, the soils were clayey and strongly weathered with a strongly acidic characteristic. Ash addition enabled to alleviate the soil acidity and to increase the amounts of nutrients of the soils, especially the surface soils. It was indicated that 1) N addition from ash to the soils was negligible, 2) the losses of nutrients by runoff water were not substantial compared with the amounts of nutrients contained in ash, 3) ash alkalinity seemed to be consumed for inactivating exchangeable AI mainly in the surface soils, and 4) development of variable negative charges could contribute to the retention of inorganic bases derived from ash. After harvest of upland rice, the soil chemical properties in the plots treated with 100 and 200 Mg ha-1 fuel returned to the levels before burning, indicating the rapid loss of nutrients due to leaching and erosion as well as the uptake by plants. However, the soils treated with 300 Mg ha^-1 fuel still showed high contents of exchangeable bases and a low content of exchangeable AI. On the other hand, the soils at the Sabal site were sandy and were characterized by a very low nutrient status. The changes in the amounts of nutrients by ash addition were similar to those at the B. Ringin site. However, the changes in the level of exchangeable AI which were not appreciable were probably due to the low AI content. It was postulated that because of the sandy texture and low CEC of the soils, inorganic bases contained in ash were only suspended in the soil solution. Taking into account the low yield of rice and low level of secondary biomass, it appeared that most of the nutrients were lost downward in soils by leaching.     

Radioactive contamination of plants in Japan covered with rain-out from H-bomb detonations in March-May 1954 at Bikini Atoll,Marshall Isls. (Part 1) Distribution of deposited radioactivity

Introduction

New H-bombs detonated from March-May 1954 at Bikini atoll not only gave unexpected disaster to crew on board No. 5 Fukuryu-Maru, a tunafishing boat which happened to be in the neighbourhood (43 miles away from) the dangerous area, but also brought a somewhat high radioactive fall-out all over Japan.     

Novel method to reduce fishy aftertaste in wine and seafood pairing using alcohol-treated yeast cells

"Fishy aftertaste" is sometimes perceived in wine consumed with seafood. Iron in wine has been reported to be a key compound that produces fishy aftertaste. However, cost-effective methods to remove iron from wine have not been developed. Here, we describe a cost-effective and safe iron adsorbent consisting of alcohol-treated yeast (ATY) cells based on the observation that nonviable cells adsorbed iron after completion of fermentation. Treatment of cells with more than 40% (v/v) ethanol killed them without compromising their ability to adsorb iron. Drying the ATY cells did not reduce iron adsorption. Use of ATY cells together with phytic acid had a synergistic effect on iron removal. We term this means of removing iron the "ATY-PA" method. Sensory analysis indicated that fishy aftertaste in wine-seafood pairings was not perceived if the wine had been pretreated with both ATY cells and phytic acid.