一次性减量施用缓控释肥对机采棉养分吸收和产量的影响

为探讨一次性减量施用缓控释肥对机采棉氮磷钾养分吸收和产量的影响,获得适宜安徽沿江棉区的机采棉最佳施肥量,根据"3414"肥料试验方案,以棉花品种'中915'为材料,设计氮磷钾3因素4施肥水平的田间肥效试验.结果表明,各施肥处理棉花单株结铃数、单铃重等均高于不施肥对照(CK),各施肥处理皮棉产量为834.83～1443....     

内侧充种式棉花穴播器的充种和清种机理

为解决内侧充种式棉花穴播器精量播种质量不高的问题,在原有穴播器的基础上,对充种口进行了改进设计,增加了辅助充种和清种装置,并对其工作原理及设计进行了阐述,同时分析了穴播器的充种和清种过程.台架试验结果表明,穴播器经改进后对棉种的播种精度有了较大提高.     

拖拉机整机控制器CCP标定系统分析与实现

针对某重型拖拉机VCU(Vehicle Control Unit,整机控制器)的参数监测与标定需求,进行了基于CAN标定协议(CAN Calibration Protocol,CCP)的标定系统开发。选取了通信硬件,编写了标定系统的底层驱动程序和上位机标定软件。采用本系统对VCU的有关参数进行了标定实验。实验结果表明,所开发的标定系统能够实现对指定参数的监测与标定功能,达到了设计要求。     

爪哇根结线虫-β1,4-内切葡聚糖酶基因cDNA全长克隆和序列分析

 以爪哇根结线虫为材料,根据同源序列法分别进行3'末端和5'末端的RACE,获得-β1,4-内切葡聚糖酶基因cDNA全长。此cDNA全长序列为1 675 bp,包括1 521 bp的完整ORF,编码一含506 aa的多肽。该基因命名为Mj-eng3-,其推导蛋白的编码氨基酸与南方根结线虫的-β1,4-内切葡聚糖酶基因MI-ENG-1、MI-ENG-3和MI-ENG-4的同源性为95.3%、95.8%和85.3%,与另外5个植物寄生线虫β-1,4-内切葡聚糖酶基因推导蛋白PP-ENG-1、GR-ENG-1、GT-ENG-1、HG-ENG-1、HS-ENG-1编码氨基酸的同源性分别为51.2%、43.7%、43.7%、46.8%和45.3%。     

Oncolytic Vaccinia Virus Harboring Aphrocallistes vastus Lectin Inhibits the Growth of Hepatocellular Carcinoma Cells

Oncolytic vaccinia virus has been developed as a novel cancer therapeutic drug in recent years. Our previous studies demonstrated that the antitumor effect of oncolytic vaccina virus harboring Aphrocallistes vastus lectin (oncoVV-AVL) was significantly enhanced in several cancer cells. In the present study, we investigated the underlying mechanisms of AVL that affect virus replication and promote the antitumor efficacy of oncolytic virus in hepatocellular carcinoma (HCC). Our results showed that oncoVV-AVL markedly exhibited antitumor effects in both hepatocellular carcinoma cell lines and a xenograft mouse model. Further investigation illustrated that oncoVV-AVL could activate tumor immunity by upregulating the expression of type I interferons and enhance virus replication by inhibiting ISRE mediated viral defense response. In addition, we inferred that AVL promoted the ability of virus replication by regulating the PI3K/Akt, MAPK/ERK, and Hippo/MST pathways through cross-talk Raf-1, as well as metabolism-related pathways. These findings provide a novel perspective for the exploitation of marine lectins in oncolytic therapy.     

Classification of Southeast Asian mints (Mentha spp.) based on simple sequence repeat markers

Mentha is a complex genus encompassing many species as a consequence of their interspecific hybridization and polyploidy. Southeast Asian mints have been poorly distinguished though they are widely used for culinary and medical purposes. In this study, we have analyzed Southeast Asian mints and known varieties as well as a related Lamiaceae species (Nepeta sp.) using simple sequence repeat (SSR) markers and leaf morphology. Two types of mints were clearly distinguished based on their venation pattern and leaf shape index. We developed 12 SSR markers that allowed good amplification in the Mentha and another Lamiaceae species. In the SSR-based phylogram, the Mentha lines could be delimited into groups I–VI. The Southeast Asian mints divided into groups I and II, and the phylogram separated most of the available species, with groups I and II containing the known species M. × cordifolia and M. arvensis, respectively. The separation of the two groups was supported by a population structure analysis. The SSR markers developed in this study enabled the simultaneous classification of mints and will help improve our understanding of the genetic composition of known mint varieties and as yet unclassified Southeast Asian mints.     

植物寄生线虫发育基因的研究进展

植物寄生线虫是危害农作物的重要病原物之一.笔者对植物寄生线虫的发育基因研究状况进行了评述.目前,关于植物寄生线虫发育基因及其调控机理研究不多,主要集中于发育相关基因的克隆,RNAi方法和原位杂交技术在线虫发育基因分析中得到成功应用.利用模式动物秀丽小杆线虫发育基因的信息和方法研究植物寄生线虫发育基因将是人们研究的热点之一.     

深圳市福田区社区公园植物景观评价

深圳社区公园建设较早，结合当前“公园城市”建设的贯彻、开展，老旧社区公园亟需更新、提质。以福田区3个不同建设时段的社区公园作为调查对象，运用IPA方法进行分析，对社区公园植物景观的满意度进行调查。并结合各阶段社区公园中乔木、灌木、地被的品种、群落及空间的研究，对各梯队的满意度-重要性进行分析，总结各梯队中部分因子满意度较低的原因。最后结合各梯队满意度分析结论，提出社区公园植物景观设计、营建相关建议。     

基于正交试验法的微型管道水轮机优化设计

针对感应水龙头使用的干电池带来的环境污染问题,设计研发出了1种微型管道水轮机,初步确定其各过流部件的形状和尺寸,对该水轮机进行多目标优化正交试验,确定了最优试验方案,并进行物理模型试验研究.该微型管道水轮机通过水流和水轮机之间相互作用,将其旋转机械能传递给发电机,产生供水龙头使用的电能.基于正交试验优化理论,在设计流量下,水轮机出力为6.30 W,与初步设计方案相比提高了32.35%,效率为85.13%,提高了2.58%,优化后水轮机出力和效率均达到设计需求.极差分析结果表明:水轮机出力随着叶片数的增加先增大后减小,随着固定导叶数的增加而增大,随着固定导叶开度的增大而减小,对称导叶叶型出力最大;其效率随着叶片数的增加而增大,随着固定导叶数的增加而增大,随着固定导叶开度的增大先增大后减小,负曲度导叶叶型效率最高.研究结果为微型管道水轮机设计和优化提供一定的参考.