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11.
Iliana Atanasova Petia Kabadjova-Hristova Katerina Stefanova Nevena Bogatzevska Penka Moncheva 《European journal of plant pathology / European Foundation for Plant Pathology》2009,124(3):451-456
Fifty strains of Erwinia amylovora isolated in Bulgaria from different host plants and locations as well as in different years were analysed by RFLP analysis
of the pEA29 PstI amplified fragment with HpaII. All the strains formed three well-resolved fragments (large—from 365 to 440 bp, medium—about 341 bp and small—about 180 bp).The
strains were classified into three RFLP groups based on the polymorphism in the length of the largest fragment. This fragment
was of intermediate size for 63% of the strains, and it was the longest (from 410 to 440 bp) for 29% of the strains. The variable
region was sequenced for five strains. The DNA sequence analysis confirmed the different size of the largest fragment. Ten
or more than ten SSRs were found for the strains in the group with the largest size of the largest fragment. Some correlation
between the RFLP profiles and the origin of the strains was revealed. The RFLP profiles displayed stability in certain strains
isolated from the same trees and orchards, but in different years. The number of SSRs was different in strains isolated from
one and the same host plant, orchard and year, and also in strains isolated from the same host plant and orchard, but in different
years. This could indicate that under natural conditions the fire blight symptoms might be caused by a mixture of E. amylovora strains with different SSR numbers, and so coexistence of distinguishable strains or a change in the population could be
assumed. 相似文献
12.
Erreguerena Ignacio Antonio Havis Neil David Heick Thies Marten Gorniak Kalina Quiroz Facundo Carmona Marcelo Anibal 《植物病害和植物保护杂志》2022,129(6):1343-1353
Journal of Plant Diseases and Protection - Ramularia leaf spot (RLS) caused by the fungus Ramularia collo-cygni (Rcc) has become a threat to barley production in Argentina. All barley varieties are... 相似文献
13.
European corn borer (Ostrinia nubilalis Hbn., Lepidoptera: Crambidae): comparing the performance of a new bisexual lure with that of synthetic sex pheromone in five countries 下载免费PDF全文
14.
Vandana Vinayak Kalina M. Manoylov Hélène Gateau Vincent Blanckaert Josiane Hérault Ga?lle Pencréac’h Justine Marchand Richard Gordon Beno?t Schoefs 《Marine drugs》2015,13(5):2629-2665
The rise of human populations and the growth of cities contribute to the depletion of natural resources, increase their cost, and create potential climatic changes. To overcome difficulties in supplying populations and reducing the resource cost, a search for alternative pharmaceutical, nanotechnology, and energy sources has begun. Among the alternative sources, microalgae are the most promising because they use carbon dioxide (CO2) to produce biomass and/or valuable compounds. Once produced, the biomass is ordinarily harvested and processed (downstream program). Drying, grinding, and extraction steps are destructive to the microalgal biomass that then needs to be renewed. The extraction and purification processes generate organic wastes and require substantial energy inputs. Altogether, it is urgent to develop alternative downstream processes. Among the possibilities, milking invokes the concept that the extraction should not kill the algal cells. Therefore, it does not require growing the algae anew. In this review, we discuss research on milking of diatoms. The main themes are (a) development of alternative methods to extract and harvest high added value compounds; (b) design of photobioreactors; (c) biodiversity and (d) stress physiology, illustrated with original results dealing with oleaginous diatoms. 相似文献
15.
Zagorov MY Draganov MM Alimanska SA Staykova ND Stefanov RS Trayancheva M Kuzmanova SI Murdjeva MA 《Comparative immunology, microbiology and infectious diseases》2007,30(3):153-162
Indirect immunofluorescent assay for antinuclear antibodies (IIFA-ANA) on HEp-2 cell substrate is a widely used test for diagnosis of connective tissue diseases. Recently, serum-free fibroblast cell line McCoy-Plovdiv has been developed to provide an alternative substrate. The aim of the present study was to evaluate the diagnostic performance of IIFA-ANA on McCoy-Plovdiv cell substrate for systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) and compare it with that of the standard HEp-2 cell substrate. Sera from 72 patients with RA and 23 patients with SLE were tested with IIFA-ANA on both substrates. The control group consisted of 100 sera from healthy individuals. The agreement (Cohen's kappa) and the diagnostic performance (ROC analysis) of both methods were evaluated. IIFA-ANA on McCoy-Plovdiv cells proved to be comparable with the standard IIFA-ANA on HEp-2 cell substrate. 相似文献
16.
Distribution of cones in human and monkey retina: individual variability and radial asymmetry 总被引:7,自引:0,他引:7
C A Curcio K R Sloan O Packer A E Hendrickson R E Kalina 《Science (New York, N.Y.)》1987,236(4801):579-582
The distribution of photoreceptors is known for only one complete human retina and for the cardinal meridians only in the macaque monkey retina. Cones can be mapped in computer-reconstructed whole mounts of human and monkey retina. A 2.9-fold range in maximum cone density in the foveas of young adult human eyes may contribute to individual differences in acuity. Cone distribution is radially asymmetrical about the fovea in both species, as previously described for the distribution of retinal ganglion cells and for lines of visual isosensitivity. Cone density was greater in the nasal than in the temporal peripheral retina, and this nasotemporal asymmetry was more pronounced in monkey than in human retina. 相似文献
17.
Ganka?GanevaEmail author Viktor?Korzun Svetlana?Landjeva Nikolaj?Tsenov Mariana?Atanasova 《Euphytica》2005,145(3):305-315
Bulgarian common wheat cultivars released in the period 1925–2003 were studied using the gibberellic acid (GA) test and microsatellite
analysis of the Xgwm261 locus on chromosome 2DS to identify the semi-dwarfing (Rht) genes. The old cultivars, isolated through selection from landraces, carried rare alleles (211- and 215-bp) at Xgwm261 locus, and those developed by hybridisation to foreign cultivars, carried the 165- and 174-bp alleles. Forty-two (55.3%)
of 76 modern cultivars were GA-responsive. The 192-bp allele, diagnostic for Rht8, was observed in 64 (84.2%) modern cultivars, of which 37 carry Rht8 alone, and 27 possess a combination of Rht8 and a GA-insensitive allele viz. Rht-B1d (17); Rht-D1b (6) and Rht-B1b (4). The 174-bp allele is present in seven cultivars, only one of which is photoperiod-sensitive, and the rest are day-length
insensitive. The 203-bp allele was found in six modern cultivars. Cultivars carrying the Rht8 allele are the most widespread and some of them have been cultivated for a long period. Cultivars with the `Saitama 27' allele
(Rht-B1d) are the most productive and are second in distribution in the country. The recently observed trend for increasing the proportion
of cultivars with GA-insensitive Rht genes is probably due to their combination with the 192-bp allele of Xgwm261 locus tightly linked to the Ppd-D1, to the break of the link between the 174-bp allele and ppd-D1, and to the introduction of other genes influencing flowering time. 相似文献