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81.
Seiji KATAGIRI Masaharu MORIYOSHI Yoshiyuki TAKAHASHI 《The Journal of reproduction and development》2013,59(6):575-579
A high incidence (about 70%) of alteration in endometrial epidermal growth factor
(EGF) profile, i.e., loss of 2 peaks on days 2–4 and 13–14, has been linked to a
reduced fertility in multiparous repeat breeder Holstein cows. However, the EGF
profile in Holstein heifers and other breeds (types) of cattle has not been
investigated. In study 1, EGF concentrations were determined using endometrial
tissues obtained by biopsy on days 3, 7 and 14 from 84 fertile Holstein heifers to
obtain a normal range and 53 repeat breeder Holstein heifers to estimate incidence of
alterations in the EGF profile. In repeat breeder heifers, EGF concentrations were
similar to fertile controls on 3 days and five animals (9.4%) had an altered EGF
profile with EGF concentrations below the normal range on days 3 and 14. In study 2,
EGF concentrations on day 3 were repeatedly examined from the nulliparous period to
the third postpartum period in 28 Holstein (dairy) and 47 Japanese Black (beef)
cattle. The effect of parity on EGF concentrations on day 3 was different between
Holstein and Japanese Black cattle. In Japanese Black cows, the EGF concentrations
were consistently high throughout the study period, while in Holstein cows, the EGF
concentrations decreased after the second calving. In conclusion, unlike multiparous
repeat breeder Holstein cows, an altered EGF profile may not be a major cause of
repeat breeding in Holstein heifers, and the peak EGF concentrations around day 3 may
decrease even in fertile populations of multiparous dairy cows, but not in beef
cows. 相似文献
82.
Yume KITAGAWA Kaho HAYAKAWA Daichi OIKAWA Kazuki IKEDA Maki IKEDA Daiki HARADA Mitsuhiro FURUSE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(4):511
In modern society, stress caused by relationships and emotions is one of the greatest social problems. Similar to humans, domestic and captive animals live under various stresses. Several stresses have been associated with skin disorders, such as atopic dermatitis, but there is a lack of reliable and objective indicators for the characterization of this association. This study aimed to define the changes in fatty acid composition and amino acid concentration in the skin following repeated restraint stress in ICR mice. Mice subjected to 30 min of daily restraint stress for 8 days showed changes in the composition of saturated fatty acids, such as an increase in palmitic acid content, which are the substrates of Δ-9 desaturase. Conversely, unsaturated fatty acids decreased with stress treatment, which appeared to be a result of these fatty acids being the substrate of Δ-6 desaturase. Changes in fatty acid composition after stress treatment may be one of the factors that cause skin inflammation. The water-retention capacity may have been lowered by stress treatment because histidine and leucine, which are natural moisturizing factors, were significantly decreased. The collagen content in the skin gradually decreased after repeated stress treatment. Our results indicate that repeated restraint stress may impact skin health through changes in both the fatty acid composition and amino acid concentration in mice. 相似文献
83.
Hideko KAMEYAMA Yoshikazu FUJIMOTO Yukiko TOMIOKA Sayo YAMAMOTO Haruka SUYAMA Hiromi INOUE Eiki TAKAHASHI Etsuro ONO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(4):574
Bordetella bronchiseptica (B. bronchiseptica) is associated with respiratory tract infections in laboratory animals. In our laboratory animal facility, B. bronchiseptica was isolated from 21 of 27 apparently healthy rabbits obtained from a breeding farm contaminated with B. bronchiseptica. Restriction fragment length polymorphism (RFLP) analysis showed that the flagellin genotype of isolates from the laboratory animal facility and breeding farm was type A, which is seen relatively frequently in rabbits in Europe. To examine its pathogenicity, guinea pigs, rats, and mice were inoculated intranasally with a representative strain isolated in the laboratory animal facility. Following inoculation of 107 colony forming unit (cfu), severe inflammation was observed in the lungs of guinea pig and mice, although the inflammation was less severe in rats. The strain was recovered from the trachea and lungs of these species after inoculation with lower dose such as 103 or 104 cfu. These results suggest that the isolated strain causes respiratory tract infection in guinea pigs, rats, and mice, and that its pathogenicity higher in mice than in rats. This study extends our knowledge of interpreting the microbiologic status of laboratory animals, which will contribute to the development of reliable and reproducible animal experiments. 相似文献
84.
Toru TSUCHIDA Yoshihiko TAKAHASHI Norikuni OHTAKE Takuji OHYAMA Masaru KUBOTA 《Soil Science and Plant Nutrition》2006,52(1):133-133
(pp. 811–816)
The application method of liquid fertilizer with irrigation water was tested in large scale paddy fields using a newly developed fertilizer supplier which can make a supplying rate of liquid fertilizer constant. Uniformity of fertilization, change in the nitrogen concentration of flooded water and absorption of applied nitrogen by paddy rice were examined. High uniformity of fertilization was achieved with this method as compared with a conventional broadcast application of granular fertilizer, or an inflow fertilization of granule-like fertilizer with irrigation water.
When urea was applied as liquid fertilizer, the fertilizer-N disappeared quickly from ponding water under shallow conditions of ponding water depth. In the case of the same depth of ponding water, the rate of reduction was large under conditions of large permeability of water.
The nitrogen utilization rates of urea and ammonium sulfate labeled with15 N were not related to the depth of ponding water at the time of fertilization. When ammonium sulfate was supplied the nitrogen utilization rate was around 50% regardless of fertilization conditions however, the urea-N utilization rate fell to 40% or less at a permeability of ponding water of 0.8 cm day−1 or less, while it increased to 50% equivalent to ammonium sulfate, at 0.8 cm day−1 or more permeability. 相似文献
The application method of liquid fertilizer with irrigation water was tested in large scale paddy fields using a newly developed fertilizer supplier which can make a supplying rate of liquid fertilizer constant. Uniformity of fertilization, change in the nitrogen concentration of flooded water and absorption of applied nitrogen by paddy rice were examined. High uniformity of fertilization was achieved with this method as compared with a conventional broadcast application of granular fertilizer, or an inflow fertilization of granule-like fertilizer with irrigation water.
When urea was applied as liquid fertilizer, the fertilizer-N disappeared quickly from ponding water under shallow conditions of ponding water depth. In the case of the same depth of ponding water, the rate of reduction was large under conditions of large permeability of water.
The nitrogen utilization rates of urea and ammonium sulfate labeled with
85.
Methane uptake and nitrous oxide emission in Japanese forest soils and their relationship to soil and vegetation types 总被引:1,自引:1,他引:1
Tomoaki MORISHITA Tadashi SAKATA Masamichi TAKAHASHI Shigehiro ISHIZUKA Takeo MIZOGUCHI Yoshiyuki INAGAKI Kazuhiko TERAZAWA Satoshi SAWATA Masanori IGARASHI Hiroshi YASUDA Yasuhiro KOYAMA Yoshihito SUZUKI Nobuyuki TOYOTA Masamichi MURO Masaru KINJO Hirokazu YAMAMOTO Daitaro ASHIYA Yoichi KANAZAWA Tetsu HASHIMOTO Hidetaka UMATA 《Soil Science and Plant Nutrition》2007,53(5):678-691
86.
Takahiro SATO Maki SEKIGUCHI Atsuko MATSUMOTO Kaho SHIMADA Mikuya IWANAGA Mitsutaka IKEZAWA Yasuko HANAFUSA Tomoyuki SHIBAHARA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(3):342
A 31-month-old Japanese Black cow (Bos taurus) aborted at 5 months of gestation with no clinical symptoms. Histopathological examination of the placenta and fetus revealed severe necrotic placentitis associated with numerous irregular degenerative fungi and inflammatory cells. Regular filamentous fungi were also detected, without inflammatory response in the fetal digestive and respiratory organs. Both fungi had aleurioconidia and septa in the placenta and fetal organs and immunohistochemically stained with antibodies against Aspergillus spp. Aspergillus terreus was isolated from the fetal lung and abomasal contents as confirmed using mycological and molecular methods. This is the first immunohistochemical, morphological, and molecular identification of A. terreus in bovine placenta and aborted fetuses. 相似文献
87.
AKIYOSHI TAKAHASHI YUTAKA AMEMIYA AKIKAZU YASUDA HIROSHI MEGURO HIROSHI KAWAUCHI 《Fisheries Science》2002,68(5):1073-1080
Several pituitary hormones, including adrenocorticotropin (ACTH), melanotropin (MSH) and β-endorphin, are generated from a common precursor protein, proopiomelanocortin (POMC). In fish, in addition to steroidogenesis of ACTH and melanogenesis of MSH, immunomodulating activity has been found in some POMC-related peptides. To investigate the functions of these peptides in the homologous system, it is necessary to establish a convenient detection method for the peptides. The present study aimed to establish a method for the detection of POMC-related peptides in bigeye tuna Thunnus obesus using a small amount of tissue sample, but not requiring peptide purification. We first determined the nucleotide sequence of tuna POMC cDNA. The cDNA was composed of 1084 base pairs (excluding the poly A tail) that encoded POMC consisting of 222 amino acids. We then fractionated an acid-acetone extract of one pituitary by reverse-phase high performance liquid chromatography (HPLC) and determined the molecular weight of each separated peptide by mass spectrometry. Consequently, we detected eight POMC-related peptides by comparing the values to the deduced amino acid sequence. Thus, the present study enabled the detection of POMC-related peptides from a small amount of tissue without the use of several purification steps. 相似文献
88.
Kei TAKAHASHI James K CHAMBERS Yuta TAKAICHI Kazuyuki UCHIDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(12):1563
Cerebral amyloid β (Aβ) deposition is a pathological hallmark of Alzheimer’s disease (AD). There are several molecular species of Aβ, including Aβ40, Aβ42, and Aβ43, and the pathological roles of Aβ43 have attracted particular attention in recent years. Aβ43 is mainly deposited as senile plaques (SPs) in AD brains, and is known to be more amyloidogenic and neurotoxic than Aβ42 and Aβ40. Aβ40 and Aβ42 deposition have been demonstrated in several animal species, while Aβ43 deposition has not been studied in animals. The brains of sea lions, dogs, and cats exhibit unique age-related Aβ pathologies. In the present study, the deposition patterns of Aβ40, Aβ42, and Aβ43 were examined immunohistochemically in the brains of aged dogs (n=52), sea lions (n=5), and cats (n=17). In dogs, most cerebral amyloid angiopathy (CAA) lesions and primitive SPs were positive for Aβ42, Aβ43, and Aβ40. However, diffuse SPs and capillary CAA lesions were negative for Aβ40. In sea lions, all SPs and most CAA lesions were positive for Aβ42, Aβ43, and Aβ40, while capillary CAA lesions were negative for Aβ40. In cats, Aβ42-immunopositive granular aggregates and arteriole and capillary CAA lesions were positive for Aβ43, but negative for Aβ40. Double-labelling immunohistochemistry revealed the co-localization of Aβ42 and Aβ43. These findings suggest that Aβ43 and Aβ42 are frequently deposited in the brains of Carnivora animals and may play an important role in Aβ pathology. 相似文献
89.
Kaoru HATATE Kazuhiko SHINYA Asako MATSUO-SATO Shuichi SASAKI Bhuminand DEVKOTA Masahiro TAKAHASHI Toichi HIRATA Norio YAMAGISHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):337-340
The fluctuations in the plasma levels of several bone markers were investigated in newborn calves. Experiment
1 monitored the postnatal changes in the plasma levels of tartrate-resistant acid phosphatase isoform 5b
(TRAP5b), total alkaline phosphatase (t-ALP) and bone-specific alkaline phosphatase (BAP) in four calves.
These markers increased significantly from 9−20 hr after the first colostrum-suckling compared with the values
immediately after birth. Experiment 2 evaluated changes in the plasma TRAP5b, t-ALP, BAP and type I collagen
cross-linked N-telopeptide (NTx) levels within 2 days post-birth in five calves with successful passive
immunization via colostrum (non-deficient group) and five others with poor colostrum intake (deficient group).
The non-deficient group had significantly higher plasma levels of the four parameters around 12 hr of life
compared with the deficient group. The results suggest that the increase in plasma bone markers in calves in
the first day of life is related to the colostrum intake. 相似文献