Responses of seasonally anovulatory mares to daily administration of thyrotropin-releasing hormone and(or) gonadotropin-releasing hormone analog

Seventeen seasonally anovulatory light horse mares were treated daily, starting January 5 (d 1), for 28 d with GnRH analog (GnRH-A; 50 ng/kg BW) and(or) thyrotropin-releasing hormone (TRH; 5 microg/kg BW) in a 2 x 2 factorial arrangement of treatments to test the hypothesis that combined treatment may stimulate follicular growth and development. Ovaries were examined via ultrasonography and jugular blood samples were collected every 3 d. Frequent blood samples were collected after treatment injections on d 1, 2, 4, 7, 11, 16, and 22; on d 29, all mares received an i.v. mixture of GnRH, TRH, sulpiride, and EP51389 (a growth hormone secretagogue) to assess pituitary responsiveness. No consistent effects (P > 0.1) of treatment were observed for plasma LH, FSH, prolactin, or thyroxine concentrations in samples collected every 3 d. The only effect on ovarian follicle numbers was a reduction in number of follicles 11 to 19 mm in diameter due to TRH treatment (P = 0.029). No mare ovulated during treatment. On the days of frequent sampling, mean LH (P = 0.0001) and FSH (P = 0.001) concentrations were higher in mares receiving GnRH-A and tended to increase from d 1 through 7. In contrast, mean prolactin (P = 0.001) and thyroid-stimulating hormone (P = 0.0001) concentrations were high in mares receiving TRH on d 1 but rapidly decreased thereafter. When mares were administered the secretagogue mixture on d 29, the LH response was greater (P = 0.0002) in mares that had previously received GnRH-A but the FSH response was not affected (P > 0.1); the prolactin response was greater (P = 0.014) and the TSH response was smaller (P = 0.0005) in mares that had previously received TRH. Surprisingly, an immediate growth hormone response to EP51389 was absent in all mares. In conclusion, daily GnRH-A treatment stimulated plasma LH and FSH concentrations immediately after injection; although no long-term elevation in preinjection concentrations was achieved, the responses gradually increased over time, indicating a stimulation of gonadotropin production and storage. Daily treatment with TRH stimulated plasma TSH and prolactin concentrations, but the response diminished rapidly and was minimal within a few days, indicating a depletion of pituitary stores and little or no stimulation of production. There was no beneficial effect of adding TRH treatment to the daily GnRH-A regimen.     

Measurement and estimation of plastic greenhouse reference evapotranspiration in a Mediterranean climate

The standard FAO methodology for the determination of crop water requirements uses the product of reference evapotranspiration (ET_o) and crop coefficient values. This methodology can be also applied to soil-grown plastic greenhouse crops, which occupy extended areas in the Mediterranean basin, but there are few data assessing methodologies for estimating ET_o in plastic greenhouses. Free-drainage lysimeters were used between 1993 and 2004 to measure ET_o inside a plastic greenhouse with a perennial grass in Almería, south-eastern Spain. Mean daily measured greenhouse ET_o ranged from values slightly less than 1 mm day⁻¹ during winter to values of approximately 4 mm day⁻¹ during summer in July. When the greenhouse surface was whitened from March to September (a common practice to control temperature), measured ET_o was reduced by an average of 21.4%. Different methodologies to calculate ET_o were checked against the measurements in the greenhouse without and with whitening. The methods that performed best in terms of accuracy and statistics were: FAO56 Penman–Monteith with a fixed aerodynamic resistance of 150 s m⁻¹, FAO24 Pan Evaporation with a constant Kp of 0.79, a locally-calibrated radiation method and Hargreaves. Given the data requirements of the different methods, the Hargreaves and the radiation methods are recommended for the calculation of greenhouse ET_o because of their simplicity.     

Production and water use in lettuces under variable water supply

The effects of a variable water supply on the water use, growth and yield of two crisphead and one romaine (i.e., Cos) lettuce cultivar were examined in a field experiment using a line source sprinkler system that produced a range of water regimes that occur in growers fields. Four locations at increasing distances from the main line were monitored through the season (i.e., from thinning to harvest, 28–63 days after planting (DAP)). These locations at the end of the season corresponded to: (1) rewatering to field capacity (FC); (2) watering with a volume 13% below that required in the field capacity treatment (0.87*FC); (3) 30% below FC (0.70*FC); and (4) 55% below FC (0.45*FC). A linear production function for dry matter accumulation and fresh weight vs. crop evapotranspiration (ET_c) was determined for lettuce during this period, giving a water use efficiency for dry matter of 1.86 g m^–2 mm^–1 and for fresh weight of 48 g m^–2 mm^–1 . For lettuce irrigated to field capacity, ET_c between thinning and harvest was 146 mm; maximum crop coefficients of 0.81–1.02 were obtained at maturity (55–63 DAP). For the three irrigation treatments receiving the largest water application, ET_c was higher in the Cos culivar than in the two crisphead lettuce cultivars which had similar ET_c. Plant fresh weight was more sensitive than dry weight to reduction in water supply. In the FC treatment, root length density and soil water extraction were greatest in the top 0–45 cm, and decreased rapidly below 45 cm depth. Soil water extraction by roots increased at lower depths when irrigation was reduced. Instantaneous rates of leaf photosynthesis and leaf water potential showed no response to the irrigation treatments in this study, despite differences in biomass production. Evaporation was determined to be the major component of ET_c for 45 of the 63 days of the growing season. The large loss of water by evaporation during mid-season and the apparent insensitivity of lettuce to the volume of irrigation during this period may provide an opportunity for reducing irrigation applications.     

Role of land-surface changes in arctic summer warming

A major challenge in predicting Earth's future climate state is to understand feedbacks that alter greenhouse-gas forcing. Here we synthesize field data from arctic Alaska, showing that terrestrial changes in summer albedo contribute substantially to recent high-latitude warming trends. Pronounced terrestrial summer warming in arctic Alaska correlates with a lengthening of the snow-free season that has increased atmospheric heating locally by about 3 watts per square meter per decade (similar in magnitude to the regional heating expected over multiple decades from a doubling of atmospheric CO2). The continuation of current trends in shrub and tree expansion could further amplify this atmospheric heating by two to seven times.     

Use of state‐space modelling to identify ecological covariates associated with trends in pinniped demography

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