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51.
Batch and upflow column leaching experiments were used to evaluate the nature and extent of Cu and Zn solubilization from contaminated soil by nitrilotriacetic acid (NTA) in 0.025 M NaClO4. In batch soil suspensions, NTA levels of 10?5 to 10?3 M substantially promoted Cu and Zn release from the metal-enriched soil. The ability of NTA to enhance Cu and Zn solubility decreased with increasing solution acidity probably due to competitive binding of NTA by protons and Fe released by hydrous oxide dissolution. However, in the pH range typically encountered in northeastern U.S. soils, soluble metal levels were nearly constant for a given NTA concentration. Leaching soil columns with NTA solutions enhanced Cu release more than Zn, as the enrichment ratio (cumulative metal leached by NTA compared to the 0.025 M NaClO4 control leachate) after 85 pore volumes displacements was 23.6 and 4.3 for Cu and Zn, respectively. While Cu release by 0.01 M CaCl2 differed little from the control, 0.01 M CaCl2 was substantially more effective than 10?5 M NTA in displacing bound Zn. The data reflect different retention mechanisms for Cu and Zn in this soil. 相似文献
52.
53.
Van Poucke C Dumoulin F De Keyser K Elliott C Van Peteghem C 《Journal of agricultural and food chemistry》2004,52(10):2803-2806
When the use of tylosin as a feed additive was forbidden by Council Regulation 2821/98, the necessity of a chemical confirmation method for the monitoring of the ban was created. Recently a method was developed for the detection of tylosin in animal feed by means of LC-MS/MS. During the validation high deviating values for the decision limit, detection capability, and repeatability for tylosin in cattle feed were observed, and the presence of urea and the formation of a tylosin urea adduct (TUA) were suggested as possible explanations. In this study two hydrolysis approaches for the TUA adduct were compared, namely, a chemical hydrolysis and an enzymatic hydrolysis with urease. The latter yielded a more complete hydrolysis of urea and was used for further validation. The recovery increased by approximately 15-25% depending on the amount of urea present in the feed (0.5-2%). The decision limit and detection capability were hardly influenced by the enzymatic hydrolysis. 相似文献
54.
Because the economics of soil extraction processes depend on conservation and reuse of costly chelating agents, the ability of various electrolytes to modify EDTA extraction of Pb from a grossly-contaminated soil (PbT=21%) was investigated using batch equilibration experiments. In the absence of added electrolyte, a single 5-hr. extraction with 0.04 M EDTA (corresponding to 1∶1 PbT to EDTA ratio) released 65% of PbT over the pH 5 to 9 range. Under these conditions, Na+-, Li+-, and NH4ClO4 salts at 0.5 M increased Pb desorption to nearly 80%, probably from exchange displacement of soilbound Pb2+ and increased solubility of Pb-containing phases at higher ionic strength. Because Cl? and ClO4 ? salts were equally effective, chlorocomplex formation was insignificant. Under slightly acidic conditions, Ca(ClO4)2 and Mg(ClO4)2 at 0.167 M caused roughly the same elevation in Pb recovery as 0.5M of the monovalent electrolytes. However, with progressively higher pH, Ca, and to a lesser extent Mg, suppressed Pb solubilization by competitive chelation of EDTA. Pb recovery by EDTA soil washing could be enhanced by addition of Ca salts at pH 4 to 6. Subsequent pH elevation in the presence of Ca would promote decomposition of Pb-EDTA complexes and separation of Pb as a hydroxide precipitate. 相似文献
55.
G A Baxter J P Ferguson M C O'Connor C T Elliott 《Journal of agricultural and food chemistry》2001,49(7):3204-3207
The development of an assay for the detection of streptomycin residues in pasteurized whole milk using an optical biosensor (Biacore) is reported. Streptomycin-adipic hydrazide coupled to bovine thyroglobulin was used to produce a sheep polyclonal antibody. The antibody displayed excellent cross-reactivity with dihydrostreptomycin (106%). There was no significant cross-reaction with other aminoglycosides or common antibiotics. Streptomycin was also immobilized onto a CM5 sensor chip to provide a stable, reusable surface. The developed assay permitted the direct analysis of whole milk samples ( approximately 3.5% fat) without prior centrifugation and defatting. Results were available in 5 min. The limit of detection of the assay was determined as 4.1 ng/mL, well below the European maximum residue limit (MRL) of 200 ng/mL. Repeatability (or coefficient of variation) between runs was determined as 3.5% (100 ng/mL; 0.5 x MRL), 5.7% (200 ng/mL; MRL), and 7.6% (400 ng/mL; 2 x MRL). 相似文献
56.
Denise A. Elliott Lesley G. King Carole A. Zerbe 《Journal of Veterinary Emergency and Critical Care》1995,5(1):17-23
Alterations in thyroid indices in critically ill dogs were studied retrospectively to determine the incidence of the euthyroid sick syndrome and to assess its prognostic relevance to survival. Sixty-seven dogs were classified as euthyroid bases on a TSH stimulation test. Forty-one of 67 (61%) of the euthyroid dogs had low baseline serum T4 concentrations (<1.5 ug/dl) and 38 of 67 (56%) of the euthyroid dogs had low baseline serum T3 concentrations (<75 ng/dl). There was no significant difference between either the baseline or the post-stimulation serum T4 concentrations when values of dogs that survived were compared with those that died. There was, however, a significant difference (P<0.05) between both the baseline and the post-stimulation values for T3 when survivors (100.3± 81 ng/dl, 143± 66 ng/dl) and non-survivors (64.2 ± 17.1 ng/dl, 96.6 ± 38.3 ng/dl) were compared. The euthyroid sick syndrome occurs commonly in critically ill dogs. Further, the extent of depression of serum concentrations of T3 may be correlated to mortality. 相似文献
57.
F Ardón A Döhring X Le Thi KF Weitze D Waberski 《Reproduction in domestic animals》2003,38(2):161-165
The goal of this study was to determine the ability of the Hanover gilt model to assess in vivo fertilizing capacity of preserved sperm and to consider whether any modifications to this model were needed. This model evaluates the fertilizing capacity of semen based on the fertilization rate, the rate of normal embryos and the accessory sperm count of 3–5‐day embryos. Its distinguishing characteristics are the use of one‐time insemination of sperm in reduced numbers, of spontaneously ovulating gilts and of ovulation detection through ultrasound examination of ovaries. Reduced sperm numbers allow for an accurate evaluation of the fertilizing potential of different semen treatments, thereby avoiding the compensatory effect of doses calibrated to maximize fertility. The model's usefulness was assessed in a trial run designed to compare the fertilizing capacity of liquid boar semen diluted into two different extenders. The diluent, the boar and the backflow, had no significant effect on any of the parameters studied. Gilts inseminated less than 24 h before ovulation had a significantly higher (p < 0.01) fertilization rate and accessory sperm cell count (p < 0.05) than those inseminated more than 24 h before ovulation. Very good/good embryos from homogeneous litters (only very good/good embryos were present) had a significantly higher (p < 0.01) accessory sperm count than those from heterogeneous litters (at least one embryo was of a different quality and/or oocytes were present). Both very good/good and degenerated/retarded embryos from heterogeneous litters had low accessory sperm numbers. This suggests that accessory sperm count is significantly related to the quality of the litter, but not to the quality of the embryo within gilts. It can be concluded that the Hanover gilt model is sensitive enough to show fertility differences (in this study, those associated with in vivo ageing of semen), while using relatively few gilts and little time. 相似文献
58.
The concentrations of cyclic 3':5'-adenosine monophosphate (cyclic AMP) and cyclic 3':5'-guanosine monophosphate (cyclic GMP) were determined in white light- and dim red light-adapted golden Syrian hamster retinas. Retinas from animals in dim red light had cyclic AMP concentrations of 9.29 +/- 2.94 pmol/mg of protein and cyclic GMP concentrations of 110.62 +/- 32.98 pmol/mg of protein. After white light adaptation, retinal cyclic AMP and cyclic GMP concentrations were reduced to 74% and 45% of the previous values, respectively. In another experiment with white light-adapted animals, the sex or method of immobilization of the animals (cervical dislocation vs sodium pentobarbital) had no significant effect on cyclic nucleotide values. 相似文献
59.
Michael Elliott Andrew W. Farnham Norman F. Janes David M. Soderlund 《Pest management science》1978,9(2):112-116
The insecticidal activities to Musca domestica L. and Phaedon cochleariae Fab. of a series of 3-phenoxybenzyl pyrethroid esters with a cyano substituent at the 2-, 6-. (R)-α-, or (S)-α-position are compared with those of the unsubstituted analogues. Only an (S)-α-substituent enhances activity; others diminish or almost eliminate it. (RS)-α-mixtures are generally less active than would be predicted from the potencies of their separate constituents. 相似文献
60.
Sheep raised and maintained under worm-free conditions were subjected to one heavy exposure and several low-level exposures of infective Ostertagia spp. larvae. The infections were terminated by treatment with thiabendazole. These sheep and not previously exposed controls were then challenged with 400 000 or 20 000 Ostertagia spp. larvae and killed after a further 8 days. In the previously exposed sheep, worm numbers, rate of worm development, and pathological effect, were greatly reduced. No significant differences were seen between sheep aged 6 and 18 months at the time of challenge. 相似文献