犬细粒棘球绦虫粪抗原夹心ELISA检测方法的建立

为建立特异性和敏感性高的检验犬细粒棘球绦虫感染的方法。用细粒棘球绦虫(简称,Eg)成虫抗原分别免疫兔和绵羊,收集高免血清,纯化的高免抗体。依据抗体夹心ELISA工作原理,以兔抗体包被,检测感染Eg、不同犬带科绦虫的实验犬和空白犬粪样,绵羊抗体扑捉抗原,HRP标记兔抗绵羊IgG(1∶8 000)催化显色,用酶标仪测定OD 405nm吸光度,用以确定其特异性和敏感性。试验结果表明,敏感性为82.69%(43/52),特异性为85.88%(140/163);粪抗原在感染细粒棘球绦虫16d后可检出,最低抗原浓度为9.7ng/mL即犬感染5条成虫时可检测出阳性。该检测方法具有较好的特异性和灵敏性,为进一步研制检测细粒棘球绦虫虫体抗原ELISA检测试剂盒奠定了基础。     

Surgical resolution of accidental intramuscular injection of Gudair® vaccine in a mare

This case report describes the accidental intramuscular administration of 20 mL Gudair® vaccine to a 7‐year‐old Standardbred mare and successful treatment of the resulting inflammatory reaction by radical surgical resection.     

Lunar surface magnetic fields and their interaction with the solar wind: results from lunar prospector

The magnetometer and electron reflectometer experiment on the Lunar Prospector spacecraft has obtained maps of lunar crustal magnetic fields and observed the interaction between the solar wind and regions of strong crustal magnetic fields at high selenographic latitude (30 degreesS to 80 degreesS) and low ( approximately 100 kilometers) altitude. Electron reflection maps of the regions antipodal to the Imbrium and Serenitatis impact basins, extending to 80 degreesS latitude, show that crustal magnetic fields fill most of the antipodal zones of those basins. This finding provides further evidence for the hypothesis that basin-forming impacts result in magnetization of the lunar crust at their antipodes. The crustal magnetic fields of the Imbrium antipode region are strong enough to deflect the solar wind and form a miniature (100 to several hundred kilometers across) magnetosphere, magnetosheath, and bow shock system.     

Decision support systems for monitoring and maintaining health in food animal populations

To mitigate the effects of risks to food safety and infectious disease outbreaks in farmed animals, animal health authorities need to have systems in place to identify and trace the source of identified problems in a timely manner. In the event of emergencies, these systems will allow infected or contaminated premises (and/or animals) to be identified and contained, and will allow the extent of problems to be communicated to consumers and trading partners in a clear and unambiguous manner. The key to achieving these goals is the presence of an effective animal health decision support system that will provide the facilities to record and store detailed information about cases and the population at risk, allowing information to be reported back to decision makers when it is required. Described here are the components of an animal health decision support system, and the ways these components can be used to enhance food safety, responses to infectious disease incursions, and animal health and productivity. Examples are provided to illustrate the benefit these systems can return, using data derived from countries that have such systems (or parts of systems) in place. Emphasis is placed on the features that make particular system components effective, and strategies to ensure that these are kept up to date.     

新疆褐牛导入美国瑞士褐牛后代犊牛放牧补饲试验

[目的]研究新疆褐牛导入杂交后代在不同生产方式下的生产性能与适应性.[方法]选择美新褐杂交F1代、F2代犊牛68头,并按品种类型、性别随机分为2组,试验组为放牧补饲生产,对照组为天然放牧生产.[结果]表明:美新褐牛F1代、F2代犊牛试验组与对照组相比日增重显著(P＜0.05),试验组F1代较F2代公牛日增重提高10％,...     

反刍动物甲烷排放调控的研究进展

甲烷是导致温室效应的重要因为,反刍动物是甲烷气体的重要释放者,因此减少反刍动物甲烷排放对提高饲料利用率和改善环境具有重要的意义.本文主要对甲烷的生成机制,甲烷排放量调控,粪便甲烷的回收等方面的研究进展进行概述.