Clinical equine dysautonomia and autonomic neuron damage.

Damage to the neurons of selected autonomic ganglia was quantified in relation to the severity of the clinical signs shown in acute, subacute and chronic cases of dysautonomia (grass sickness). No connection between the clinical severity of acute or subacute dysautonomia and the amount of neuronal damage in the superior cervical, stellate and coeliaco-mesenteric ganglia could be demonstrated. However, a higher proportion of normal neurons were found in chronic cases. Jejunal submucosal neuronal damage was correlated with clinical severity but further work is required to confirm this finding and to establish how widespread the alimentary neuronal lesions are in dysautonomia of different severities.     

An outbreak of infectious chicken anemia in fattening chickens in Switzerland]

An outbreak of infectious anaemia in 46 broiler flocks due to chicken anaemia agent (CAA) is described. The vertically acquired infection led to increased mortality (3.6-19.8%) in 16 to 24 day-old broiler chickens. At necropsy severe atrophy of thymus and anaemia with pale bone marrow was observed. The histologic findings were depletion of cortical thymocytes and of hematopoietic cells in the bone marrow. The CAA was isolated from 15 of 35 examined broiler flocks.     

Coagulopathic Effects and Therapy of Brodifacoum Toxicosis in Dogs

The clinical signs and laboratory changes of brodifacoum (BDF) intoxicated dogs and their response to vitamin K1 treatment were examined. Brodifacoum, a second-generation anticoagulant rodenticide, was fed to four dogs for 3 consecutive days producing a cumulative dose of 1.1 mg BDF/kg body weight. Clinical observations of the animals were made daily throughout the study. Monitored laboratory parameters included: one-stage prothrombin time (OSPT), activated partial thromboplastin time (APTT), activated coagulation time (ACT), complete blood counts, thrombocyte counts, and serum chemistry values. Response to vitamin K1 therapy was evaluated clinically and by laboratory tests. Serum BDF concentrations were monitored. Inappetence and hemorrhagic tendencies were exhibited by day 5 postrodenticide exposure. One-stage prothrombin time, APTT, and ACT were 25% greater than time zero values at 24, 24, and 72 hours postdosing, respectively. All laboratory parameters returned to normal within 48 hours of initiating vitamin K1 therapy (0.83 mg/kg orally, TID for 5 days). Serum brodifacoum concentrations were highest (1065-1215 ng/mL) during the 3 days after BDF dosing and were detectable (3.0-7.5 ng/mL) until day 24 postexposure. A mean BDF elimination half-life of 6 +/- 4 days was observed.     

Flow cytometric analysis of T cell response in mice infected with Cowdria ruminantium.

A 3-fold increase in the numbers of Lyt-2+ T cells in the circulating blood of mice infected and re-infected with the Welgevonden stock of Cowdria ruminantium, as determined by flow cytometry, is supportive evidence that immunity in heartwater is cell-mediated. The rise in Lyt-2+ cells only after re-infection of the mice is further evidence that the development of immunity in heartwater is dependent on the unhindered and adequate replication of C. ruminantium.     

A model for long-term sampling of lymph from the jejunal lymphatic trunk in pigs and sheep

Eine Methode zur langfristigen Lymphsammlung aus der jejunalen Lymphbahn bei Schweinen und Schafen Es wird eine Methode zur langfristigen Lymphsammlung im Dünndarmbereich von Schweinen und Schafen vorgestellt. Mit Hilfe eines chirurgischen Eingriffs wurde ein Silikon-Katheder im truncus lymphaticus jejunalis implantiert. Zusätzlich wurde ein zweiter Katheter in die hintere vena cava in Richtung Herz eingesetzt. Beide Katheter wurden sofort miteinander verbunden, um die Wiedereinführung der jejunalen Lymphe in den Blutkreislauf zu gewährleisten. Der negative Druck in der hinteren vena cava im Thoraxteil förderte den Lymphfluß durch das Katheter im truncus lymphaticus jejunalis. Der preprandiale Lymphfluß des Jéjunums betrug bei Schweinen 0,3 ml/h/kg Körpergewicht und stieg postprandial auf 0,78 ml/h/kg an. Beim Schaf betrug der Basiswert 0,75 ml/h/kg. Er stieg nach intraduodenaler Infusion von Rapsöl auf 4,75 ml/h/kg. Eine mechanische Stimulation der intestinalen Receptoren durch 0,9% NaCl-Lösung stimulierte ebenfalls den Lymphfluß.     

Changes in some biochemical indicators of bone turnover after ultraviolet irradiation of dairy cows

The effect of ultraviolet irradiation on some biochemical indicators of bone turnover in dairy cows was determined. The irradiation was performed using a stationary system for two months and comprised a regimen of 10 days irradiation followed by 10 days rest. After ultraviolet irradiation, significant differences in the activities of serum alkaline phosphatase (P < 0.001) and bone alkaline phosphatase isoenzyme (P < 0.05) and concentration of osteocalcin (P < 0.01) were demonstrated. The results suggest that supplementary ultraviolet irradiation during winter could be used as a simple but reliable method of preventing the development of generalised metabolic bone disorders in dairy cows.     

Comparison of disease progress curves for yam anthracnose (Colletotrichum gloeosporioides)

The incidence and severity of foliar anthracnose, caused by Colletotrichum gloeosporioides , were recorded for seven fields of yams ( Dioscorea alata var. White Lisbon) in Barbados over a complete growing season. A scoring system was developed to estimate severity, placing the emphasis on the earliest, least visible stages of the disease. Disease progress curves were described in terms of the logistic model and the effects of site and leaf age on the parameters as well as on disease at tuber bulking were analysed. Differences between sites indicated that disease was more severe in areas of higher rainfall. In severe epidemics, disease predominated on young leaves. Fungicides applied sufficiently early can delay the onset of disease and slow the rate of disease progress.