Comparative antigenic analysis of extracellular proteins of Bacteroides nodosus isolated from virulent and benign ovine footrot

Antigens in the extracellular protein (ECP) complexes of Bacteroides nodosus, isolated from sheep with either benign or virulent footrot, were studied by immunoelectrophoresis (IEP). Rabbit antisera against ECP from virulent and benign strains, were used in homologous and heterologous crossed IEP. Four precipitin peaks unique to the virulent strain, and five peaks unique to the benign strain were identified. In an attempt to characterize the different antigens in ECP, rabbit antisera were raised against an outer membrane protein (OMP, mol. wt. 35 000 daltons), pili and various proteases of virulent and benign strains of B. nodosus. No precipitin band was observed when ECP from both B. nodosus strains were reacted against anti-OMP and anti-pilus antisera. However, single precipitin bands unique to one protease from the benign strain and one protease from the virulent strain were identified. The results suggest that specific antigens other than proteases or pili are important in determining whether a B. nodosus isolate is virulent or benign.     

Neutrophil function in the dog: shape change and response to a synthetic tripeptide

Isolated dog neutrophils exposed to known chemotactic factors assumed a bipolar configuration in suspension. This response was initiated within 2 minutes with a gradual return to spherical shape. The rate of return to sphere was positively correlated with the dose of stimulant. Dog neutrophils exposed to N-formylmethionyl-leucyl-phenylalanine (fMLP) did not adapt to bipolar conformation and did not bind radiolabeled fMLP. These findings are consistent with other species (pig and cow) that also did not respond to fMLP.     

Crystalluria. Observations, interpretations, and misinterpretations

Crystalluria results from oversaturation of urine with crystallogenic substance. However, oversaturation may occur as a result of in vitro as well as in vivo events. The microscopic appearance of crystals only represents a tentative identification of their composition because variable conditions associated with their formation, growth, and dissolution may alter their appearance. Definitive identification is dependent on physical methods such as optical crystallography, x-ray diffraction, and electron microscopic analysis.     

齐卡商品肉兔在不同饲养条件下的育肥效果

作者在不同条件下，采用全窝育肥法，重复两年、两个季节测定了齐卡商品肉兔的育肥效果；探讨了温度、季节、营养水平、育肥始重对育肥效果的影响。结果表明：在试验场育肥２５０只齐卡商品兔，９０日龄育肥重２．３３～２．５３公斤，日均增重３１克以上，耗料指数３．３１：１，育肥成活率９５％以上，屠宰率５２％，净肉率８０％，肉骨比５．３５：１，兔肉品质正常。在农村条件下育肥１８０只，９０～１００日龄育肥重２．２～２．４公斤，耗精料指数２．５５：１，育肥成活率８８％～９３％。饲养齐卡商品兔与饲养二元杂交兔相比，每只可增加经济收入２０％以上。育肥季节和始重明显影响育肥未重（Ｐ＜０．０５）。依据本试验，作者建议齐卡商品兔适宜３５日龄断奶，育肥始重应不低于６００克；寒冷季节育肥，育肥第一月的室温应不低于１０℃。育肥适宜营养水平建议消化能（ＤＥ）２６００～２７００大卡／公斤，粗蛋白（ＣＰ）１８％，粗纤维（ＣＦ）１２％以上。     

溶剂法制歧化松香

将松脂或松香溶于溶剂中，Ｃ Ｐｄ／Ｃ为催化剂，≤２００℃反应，可得色泽浅与不皂化物含理低的歧化松香。对该歧化松香组成研究后表明，在上条件下除含有通常的少量脱羧产物外，尚有降解产物，它带羧基，分子量为２７６，没有其它小分子降解产物，因而收率较高，不皂化物少。     

Variability of serum bile acid concentrations over time in dairy cattle, and effect of feed deprivation on the variability.

Twelve nonlactating dairy cows, free of signs of liver disease and with normal serum activities of liver-derived enzymes and normal liver biopsy tissue, were examined over a 72-hour period for serum total bile acid concentrations. The cattle were fed hay twice daily, and blood samples were obtained every hour for 24 hours, every other hour for 24 hours, then every hour for 24 hours. After 3 weeks, the study was repeated on 6 of the cattle, thus providing data for eighteen 72-hour periods. Serum bile acid concentration varied greatly over the 72 hours, with the range being from one third to 3 times the median. There were variations by as much as 60 mumol/L from 1 hour to the next. After another 3 weeks, 8 of the cattle were deprived of hay for 48 hours and then fed hay morning and afternoon of the third (last) day of the study. There was no significant reduction in bile acid concentration after withholding the hay, but the variability was reduced (P = 0.02) during the last 20 hours of the hay-deprivation period. In 3 ancillary studies, serum bile acid concentrations were examined over a 48-hour period in 2 cows in early lactation, 3 cows in midlactation, and two 6-month-old heifers. The cows were fed hay and grain twice daily, and the heifers were fed only hay twice daily. In comparison with values for the 12 nonlactating cows fed hay twice daily, mean serum bile acid concentration in the recently freshened cows was significantly (P < 0.002) higher (62.9 vs 22.0 mumol/L).(ABSTRACT TRUNCATED AT 250 WORDS)     

Monoclonal antibodies to bovine viral diarrhea virus: cross-reactivities to field isolates and hog cholera virus strains.

Monoclonal antibodies to bovine viral diarrhea virus (BVDV) were examined for binding with a large number of North American BVDV isolates and eight strains of the serologically related pestivirus, hog cholera virus (HCV). No single BVDV monoclonal antibody reacted with all BVDV isolates. The most cross-reactive monoclonal antibody was an anti-p80/p125 antibody which showed a positive reaction with 173 of 180 (96%) North American isolates. From a fewer number of isolates tested, one anti-gp53 monoclonal antibody also showed a high cross-reactivity (94%). All BVDV isolates showed a positive reaction with at least one of the seven monoclonal antibodies in the panel. Thus, the results indicated that a pool of these monoclonal antibodies may be used in place of polyclonal antisera for the detection of BVDV contamination of cell lines or for virus isolation. For HCV, all three anti-p80/p125 monoclonal antibodies reacted positively with all eight virus strains. In contrast, none of the anti-gp53 monoclonal antibodies were reactive to HCV strains. Thus, the anti-gp53 monoclonal antibodies may be useful for distinguishing between usually innocuous BVDV infections and the highly significant HCV infections in swine for foreign animal disease surveillance.