Relationships Between Blueberry Flower Age, Pollination, and Conidial Infection by Monilinia vaccinii-corymbosi

ABSTRACT Monilinia vaccinii-corymbosi infects open blueberry flowers via the gynoecial pathway, leading to mummification of the developing fruit. To determine the effect of flower age on infection, stigmata were inoculated with conidia of M. vaccinii-corymbosi between 0 and 5 days after anthesis, fungal growth rates through the stylar canal were measured in detached flowers in the laboratory, and fruit disease incidence was determined in plants grown in the greenhouse. Hyphal growth rates were greatest in flowers inoculated on the day of anthesis, declined linearly with increasing flower age at inoculation (r = 0.921; P < 0.0001; n = 12), and were unaffected by the presence or absence of pollen applied at the time of inoculation. In greenhouse-grown plants, the percentage of infected fruit decreased exponentially with increasing flower age at inoculation (R = 0.878; P = 0.0057; n = 10), with disease incidence ranging from 76.4% for flowers inoculated on the day of anthesis to 15.5% for those inoculated 4 days later. Fruit disease incidence in the greenhouse was linearly correlated with hyphal growth rates in detached flowers (r = 0.985; P < 0.0001; n = 9), justifying the use of detached flowers when investigating gynoecial infection by M. vaccinii-corymbosi. In separate experiments, the effects of timing and sequence of pollination and inoculation on hyphal growth rates through the stylar canal and on disease incidence were investigated. Application of pollen to detached flowers 1 or 2 days before inoculation reduced hyphal growth rates by between 14.0 and 42.9% compared with flowers that received pollen and conidia simultaneously. Similarly, reductions in fruit disease incidence by between 9.5 and 18.3% were observed on greenhouse-grown plants for pollination-to-inoculation intervals ranging from 1 to 4 days. These results document that newly opened flowers are most susceptible to infection by M. vaccinii-corymbosi and that fruit disease incidence is reduced if pollination occurs at least 1 day before inoculation. Strategies that lead to early pollination of newly opened flowers may be useful for managing mummy berry disease in the field.     

Evaluation of the nutritive value of Leucaena leucocephala leaf meal, inoculated with fish intestinal bacteria Bacillus subtilis and Bacillus circulans in formulated diets for rohu, Labeo rohita (Hamilton) fingerlings

Nine isonitrogenous (35% crude protein approximately) and isocaloric (18.37 kJ g^?1) experimental diets (RLL20–BCFL40) were formulated with either raw or treated (inoculated with fish intestinal bacteria) Leucaena leucocephala leaf meal at 20%, 30% and 40% levels replacing other ingredients partially from a fish meal based reference diet (RD). Two specific strains of fish intestinal bacteria, Bacillus subtilis (isolated from Cyprinus carpio) and B. circulans (isolated from Oreochromis mossambicus) having extracellular cellulolytic and amylolytic activities, were used to inoculate Leucaena leaf meal for 15 days at 37°C. The crude fibre, cellulose and hemicellulose contents and the antinutritional factors, tannin, phytic acid and mimosine in the leaf meal decreased due to inoculation. However, free amino acids and fatty acids increased in the treated leaf meal. The response of rohu, Labeo rohita, fingerlings fed the experimental diets for 80 days was compared with fish fed a RD. Both the inclusion level and type of Leucaena leaf meal in diets significantly affected the growth performance of rohu. Fish fed diets containing inoculated Leucaena leaf meal performed better in comparison with those with the RD. On the basis of growth response, feed conversion ratio, protein efficiency ratio and apparent net protein utilization, diet formulated with 30%Leucaena leaf meal inoculated with B. circulans resulted in the best performance of rohu fingerlings followed by diet with 40%B. subtilis inoculated Leucaena leaf meal. The apparent protein digestibility (APD) was better in fish fed diets containing B. circulans inoculated leaf meal. An increasing level of raw Leucaena leaf meal was associated with a decrease in the carcass protein content of rohu fingerlings. The activity of α‐amylase increased with the increasing level of treated leaf meal in diets. Cellulase activity increased with increasing level of inclusion of raw leaf meal, and was comparatively lower in fish fed diets with treated leaf meal. Activities of protease and lipase were higher in fish fed the RD. The results showed that it is possible to incorporate Leucaena leaf meal inoculated with enzyme‐producing fish intestinal bacteria in carp diets up to 40% level of inclusion.     

Potential carbon stock in Japanese forest soils – simulated impact of forest management and climate change using the CENTURY model

Forest management and climate change may have a substantial impact on future soil organic carbon (SOC) stocks at the country scale. Potential SOC in Japanese forest soils was regionally estimated under nine forest managements and a climate change scenario using the CENTURY ecosystem model. Three rotations (30, 50, 100 yr) and three thinning regimes were tested: no‐thinning; 30% of the trees cut in the middle of the rotation (e.g. 15 year in a 30‐yr rotation) and thinned trees all left as litter or slash (ThinLef) and the trees from thinning removed from the forest (ThinRem). A climate change scenario was tested (ca. 3 °C increase in air temperature and 9% increase in precipitation). The model was run at 1 km resolution using climate, vegetation and soil databases. The estimated SOC stock ranged from 1600 to 1830 TgC (from 6800 to 7800 gC/m²), and the SOC stock was largest with the longest rotation and was largest under ThinLef with all three rotations. Despite an increase in net primary production, the SOC stock decreased by 5% under the climate change scenario.     

Anthropogenic effects on the physical and chemical properties of subtropical coastal urban soils

We investigated the effects of various anthropogenic factors on urban soil properties in subtropical, coastal Tampa, FL, USA. Specifically, we explored the influence of (i) urbanization as measured by land use, land cover, population density and years since urban development and (ii) socioeconomic conditions as reflected in household income and property values on bulk density (BD) and several key soil chemical properties. Results indicate that Tampa’s urban soils were affected to varying degrees by these factors with chemical properties being more variable than BD. Across land uses significant differences were found for Mehlich‐1 (M1) extractable P, Ca and Na. A similar trend was observed for land‐cover classes, although significant differences were also found for pH and M1‐Cu. Soil properties had no statistically significant relationship with population density. However, time since urbanization did with M1‐P and Na varying significantly across age categories. For our socioeconomic analyses, M1‐K and Mg levels differed significantly by household income while pH, P, Ca and Na values differed significantly by property value. Overall, our findings indicate that despite their inherent heterogeneity, there are identifiable patterns among subtropical coastal urban soil properties. We suggest that a more thorough understanding of these patterns and their drivers is an essential first step towards developing soil management strategies aimed at maintaining environmental quality and ecosystem services in subtropical cities.     

Soil conservation on road embankments using palm‐mat geotextiles: field studies in Lithuania

The application of geotextile mats constructed from the palm leaves of Borassus aethiopum (Borassus) and Mauritia flexuosa (Buriti) was investigated in field experiments. The use of geotextiles caused improved soil moisture storage during dry summer periods on a steep (21–25°) roadside slope in Lithuania. The enhanced soil moisture under the Borassus and Buriti mats encouraged better root development of perennial grasses, increased the number and weight of earthworms and increased the dry biomass of perennial grasses by 50.5 and 18.2%, respectively, compared with a grassland control. The mean rate of water erosion from bare soil during the study period from 17/04/2007 to 11/12/2008 was 33.21 Mg/ha. The cover of palm‐mat geotextiles decreased soil losses from bare fallow soil by 94.8–91.1%. An erosion rate of 0.85 Mg/ha over this 21‐month period was measured on the slope under perennial grasses without geotextile cover. Application of geotextile cover on perennial grasses completely prevented soil erosion by water. Therefore, use of geotextiles has clear soil and water conservation benefits on industrial slopes susceptible to erosion. The use of geotextiles has multiple benefits including soil conservation, the improvement of plant growth conditions and the encouragement of earthworm populations.     

Soil nitrogen and denitrification potential as affected by land use and stand age following agricultural abandonment in a headwater catchment

Changes in vegetation and soil properties because of agricultural abandonment may affect soil nitrogen (N) and associated processes. We investigated soil N (total N: TN, inorganic N: NH₄–N and NO₃–N) and denitrification potential in cropland, pine plantations and abandoned agricultural land along a secondary succession sequence (grassland→shrubland→secondary forest) in a headwater catchment in the Qinling Mountains, northwest China. The results show that the soil denitrification potential differed significantly among the five land‐use types with the highest potential in the secondary forest, followed by grassland, shrubland, cropland and plantations. The denitrification potential of the 20‐ to 40‐cm layer was significantly lower compared with the topsoil (0–20 cm) across all land‐use types. TN, soil organic matter (SOM) and NH₄–N increased significantly with stand age, whereas there was an opposite trend in soil pH. However, the denitrification potential did not relate to stand age in a linear manner. We conclude that changes in soil TN, SOM and pH during vegetation succession following agricultural abandonment are critical controls on the denitrification potential.     

Soil organic‐C accumulation and N availability under improved pastures established in Mediterranean oak woodlands

Long‐term effects of improved pasture establishment (with high proportion of legumes) on soil organic‐C status and N availability in Mediterranean cork oak (Quercus suber L.) woodlands were assessed. Soils were sampled beneath scattered crowns and in open areas, considering two systems: unmanaged and managed woodlands where improved pastures were installed 26 and 32 years ago. Total and labile C and N pools were measured and C and N mineralization were determined over 24 weeks laboratory incubation. Soils under improved pastures showed higher organic‐C, total‐N and net N mineralization than those under unmanaged pasture, mainly when established beneath trees. Potentially mineralizable C, C mineralization rate and microbial C were not statistically different between the unmanaged and improved pasture sites, but were higher closer to the tree than in the open area (1.8, 1.2 and 1.2 times, respectively). The qCO₂ was higher in improved pastures (1.7 times). Labile pool of C and N extracted with hot water increased under improved pasture (3.4 and 1.7 times, respectively). Results indicate that soil quality amelioration by improved pastures is stronger in the presence of oak trees. Management systems that favour oak tree maintenance and regeneration should be taken into account to reverse soil degradation.     

Using the VS‐Fast methodology for soil degradation assessment: a case study from Senegal

Soil degradation threatens sustainable food production and accelerates global warming. Poorer countries, whose agricultural sectors are highly dependent on their natural resource bases, are hit particularly hard by declining soil productivity. Calls for soil‐quality monitoring are therefore, justified and this could inform decision‐makers on the preparation of appropriate interventions. However, the provision of monitoring methodologies is not an easy task. Soil degradation affects several soil characteristics that at larger scales cannot be evaluated with models or remote‐sensing techniques. Therefore, this study focuses on investigating the use of field‐based soil assessment methodologies to differentiate degrees of soil degradation. Specifically, we test the Visual Soil Field Assessment Tool (VS‐Fast) for detecting and monitoring soil degradation using a cross‐section of 71 sites in Senegal, the soil quality of which were classed by local experts. We found low correlation between VS‐Fast classes and expert assessments. By using an ordered logit model to quantify class boundaries, we show that experts categorized areas as ‘degraded’ for a wider range and higher VS‐Fast scores than the corresponding VS‐Fast class. Yet, from general linear models and analysis of variance procedures we found that areas classed by experts as ‘degraded’ had statistically significant lower VS‐Fast scores compared to those that were judged as ‘normal’ and ‘good’, while differences of the VS‐Fast scores between the latter two were negligible. It is remarkable that the visual assessment, the cheaper component of the VS‐Fast score, performs better in differentiating degradation status than its measured counterpart. The results support the need to investigate the applicability of other VSA methodologies that only use field observations and tactile methods.     

Effects of grazing strategy on limiting nitrate leaching in grazed grass‐clover pastures on coarse sandy soil

Urinations of ruminants on grazed pastures increase the risk of nitrate leaching. The study investigated the effect of reducing the length of the grazing season on nitrate leaching from a coarse sandy, irrigated soil during 2006–2007 and 2007–2008. In both years, precipitation was above the long‐term mean. The experiment was initiated in a 4‐yr‐old grass‐clover sward in south Denmark. Three treatments were as follows grazing only (G), spring cut followed by grazing (CG) and both spring and autumn cuts with summer grazing (CGC). Nitrate leaching was calculated by extracting water isolates from 80 cm depth using ceramic suction cups. Because of considerable variation in measured nitrate concentrations, the 32 installed suction cups per treatment were insufficient to reveal differences between treatments. However, weighted nitrate leaching estimations for G, CG and CGC showed estimated mean nitrate N concentrations of 23, 19 and 13 mg/L for an estimated proportion area occupied by urine patches of 0.33, 0.26 and 0.16, respectively. Thus, N concentrations in G and CG exceeded the EU limit of 11.3 mg N/L. Under the prevailing conditions, the time of urination did not appear important. The estimated background leaching calculated from suction cups presumably not situated under urine patches resulted in mean nitrate N concentrations of 2.6 mg/L.     

Impact of Air Pollutant Emissions on the Ecosystems in the Vicinity of Industrial Areas of Indian Sub-Tropics

An investigation was conducted near the industrial areas of West Bengal, Indian subtropics to study the effect of emissions through various factories and thermal power plants on the quality of water in lakes, tanks, ponds, even well waters in relation to its use in the welfare of the society (agricultural use, psciculture and other domestic uses). The results showed that intensity of acidity (water pH) as well as concentration of various soluble salts and BOD and COD in the vicinity of an industrial area (i.e. within 100 metres) were very low. A substantially higher acidity and ion concentration were recorded up to 1 km away from industry, where the growth of aquatic plants, organisms and the production of fish (in lakes and tanks) were severely affected. The results further indicated that the acidity of water gradually increases up to pH 5.4 and concentration of sulphates and chlorides (880 and 570 mg 1^?1 respectively) between 1–2 km from the industriy, suggesting that aquatic ecosystems and domestic, agricultural and fish production are less affected with increasing distance from industry.