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81.
Takeya?Yoshioka Yasunori?Kinoshita Sanae?Kato Young?-Je?Cho Kunihiko?KonnoEmail author 《Fisheries Science》2005,71(1):213-219
ABSTRACT: Incubation of squid mantle muscle homogenate caused a selective cleavage of myosin into heavy meromyosin (HMM) and light meromyosin (LMM). HMM was isolated from the incubated homogenate by using ammonium sulfate fractionation. The purified HMM retained two types of light chain components. Its Mg2+ -ATPase activity with or without F-actin showed a Ca-sensitivity. HMM was cleaved into subfragment-1 and subfragment-2 upon chymotryptic digestion with or without Ca2+ , possessing different light chain composition. Two types of light chain component were kept intact when digested in the presence of Ca2+ . Ca2+ stabilized HMM especially in a bound form to F-actin. 相似文献
82.
Katsuhiko Yoshizawa Yuko Emoto Yuichi Kinoshita Ayako Kimura Norihisa Uehara Takashi Yuri Nobuaki Shikata Airo Tsubura 《Journal of toxicologic pathology》2013,26(1):61-66
Uterine deciduomas were found in two female virgin rats, a 15-week-old Lewis rat and a 7-week-old Sprague-Dawley rat. The firm white nodules were located at the base of unilateral uterine horns and were approximately 6 mm and 4 mm in diameter. Histopathologically, the nodules were composed of three areas, each with a distinct type of proliferating cells: large epithelioid decidual cells with round nuclei, prominent nucleoli and abundant eosinophilic cytoplasm (antimesometrial region); compact spindle-shaped cells with oval nuclei and vacuolar cytoplasm (transitional region); and pleomorphic and spiny cells with round to oval nuclei and compact eosinophilic cytoplasm (mesometrial region). These cells proliferated in sheet-like arrangements and transformed into the other types of cells located in surrounding regions. Immunohistochemically, proliferating cells in all regions were strongly positive for proliferating cell nuclear antigen. The proliferating cells were positive for vimentin, and large decidual cells were positive for common acute lymphoblastic leukemia antigen 10, a marker of uterine interstitial cells. Large decidual cells were positive for α-smooth muscle actin and desmin, suggesting differentiation into muscular cells. Progesterone receptor was expressed in all cell types; however, estrogen receptor α was not expressed in the antimesometrial region. These extremely rare tumor-like nodules represent nonneoplastic lesions referred as decidual reactions of endometrial interstitial cells, and their biological behavior is that of a space-occupying benign tumor in young rats. Our cases might provide information as a historical control in toxicity and pharmacological studies in rats. 相似文献
83.
The thermal inactivation of Ca2+ ATPase of scallop myofibrils (0.1 M KCl, pH 7.5) was found to be unaffected by the presence of Ca2+. Monomeric myosin content and salt solubility decreased much faster than Ca2+ ATPase inactivation in both Ca and EDTA media, which was well explained by faster denaturation of the rod portion than subfragment-1 of myosin. In contrast, when the myofibrils were heated at 0.5 M KCl, a slow decrease in salt solubility was observed, which was also explained by slow denaturation of the rod portion of myosin. Myofibrils from scallop smooth muscle showed the same denaturation pattern as those from adductor muscle. These results show that mollusk myosin is not always stabilized by Ca2+. 相似文献
84.
Nobuhiko Ojima Miyuki Mekuchi Toshinao Ineno Koichi Tamaki Akio Kera Shigeharu Kinoshita Shuichi Asakawa Shugo Watabe 《Fisheries Science》2012,78(5):1051-1057
Rainbow trout Oncorhynchus mykiss is a cold-water aquaculture species, and a thermally selected strain has been developed by multigenerational high-temperature breeding in Japan. We examined the expression of heat-shock proteins as candidates responsible for thermotolerance in rainbow trout using F2 offspring from F1 hybrids produced between thermally selected and normal strains. From F2 offspring, two groups were selected for western blot analysis, namely, low- and high-thermotolerance groups (times to loss of equilibrium were <30 and ??60?min, respectively). We demonstrated that the expression levels of Hsp70, Hsp60, and Hsp40 in tail fin tissues were significantly higher in the individuals with high thermotolerance than in those with low thermotolerance under non-heat-shock conditions. In particular, Hsp70 was expressed only in the individuals with high thermotolerance. These results suggest that Hsp70 is a major protein responsible for conferring thermotolerance in rainbow trout. 相似文献
85.
Hideki Fujisawa Zean Zhang Wei Sun Mingshu Huang Junichi Kobayashi Hitoshi Yasuda Yuichi Kinoshita Ryo Ando Kazutoshi Tamura 《Journal of toxicologic pathology》2012,25(3):215-219
Morphological and immunohistochemical examinations were carried out on the pancreas of a
hyperglycemic 5-year-old male cynomolgus monkey. Body weight gradually decreased from 6
months before termination, accompanying a slight reduction in food consumption and
anorexia for the last 2 days. The blood glucose level was markedly elevated when examined
at termination. Histopathologically, in the exocrine pancreas, diffuse hyperplasia of
centroacinar and intercalated duct cells and diffuse atrophy of acinar cells with sporadic
apoptosis were observed, although most centroacinar and intercalated duct cells were
proliferating cell nuclear antigen (PCNA)-positive in both the present case and
age-matched control animals. In the endocrine pancreas, the islets tended to be
hypertrophic, with an increase in insulin-positive cells in comparison with the
age-matched control animals. PCNA-positive cells also tended to increase in the islets,
although positive cells for phospho-histone H3, a marker for mitotic cells, were not
detected in the endocrine and exocrine pancreas. Moreover, neither inflammation nor
amyloidosis was noted in the islets. In conclusion, the present case probably suffered
from early-stage type 2 diabetes mellitus, and it provides fundamental information
concerning pancreatic histopathology under insulin-related derangement in monkeys. 相似文献
86.
T Sasaki K Yoshizawa Y Kinoshita H Miki A Kimura T Yuri N Uehara A Tsubura 《Journal of toxicologic pathology》2012,25(2):179-182
An intracranial lipomatous hamartoma was found in the third ventricle of a 7-week-old female BALB/cAnNCrlCrlj mouse. The nodule was composed of mature white adipose cells, which contained one large fat droplet, and there was no evidence of cytological atypia. The brain parenchyma at the retrosplenial granular cortex and the hippocampus in the cerebrum were slightly compressed, and the choroid plexus was dislocated downward. Scattered capillary vessels penetrated the nodule from the surrounding tissue. Based on these findings, the lesion was diagnosed as a lipomatous hamartoma that occurred from the roof of the third ventricle. This extremely rare tumor-like nodule represents an overgrowth of the mature adipocyte population as a malformation rather than a true neoplasm. 相似文献
87.
Physiologic changes occurring in the thyroid in response to the estrous cycle have been noted in companion animals. However, in bottlenose dolphins, the influence of different reproductive states on thyroid morphology remains unclear. Sonography was used to evaluate the variations of thyroid morphology for nine consecutive estrous cycles of four sexually mature, female Indo-Pacific bottlenose dolphins (Tursiops aduncus). The estrous cycle was divided into two phases for evaluation: the follicular and luteal phases. To compare changes associated with sex, thyroid volumes were measured in the different phases during the estrous cycle of female dolphins and were compared to the ultrasound examinations of the four male dolphins measured over a 2-mo period. In males, there was no significant difference in thyroid volume during the study period (P > 0.05). The thyroid volume measured in combining all estrous cycle phases of the female dolphins was significantly larger than that measured in the male dolphins (P < 0.05). A difference in thyroid volume during the estrous cycle was observed, with the thyroid volume during the follicular phase significantly smaller (P < 0.05) than that of the luteal phase, and is possibly related to the influence of female sex steroids. Thyroid volume variability during estrus should, therefore, be taken into account when examining the thyroid gland of female dolphins. 相似文献
88.
A protease produced by Staphylococcus aureus, isolated from a chicken suffering from dermatitis, was purified by successive precipitation with ammonium sulfate, ion-exchange chromatography on Q-Sepharose FF, Sp-Sepharose FF and Mono-Q columns. By Mono-Q column chromatography, two proteases (protease 1 and 2) were obtained. The molecular weights of protease 1 and 2 were estimated at 23.1 and 22.7 kDa, respectively, by SDS-polyacrylamide gel electrophoresis. Their isoelectric points were 5.85 and 5.55, respectively, and they possessed antigenic similarity when examined by the immunoblotting. The N-terminal amino acid sequences of both the proteases were identical (RAQYVNQLKNFKIRETQ). The activities of both the proteases were strongly increased by reducing agents such as L-cysteine and sodium thioglycolate. Their activity was inhibited by thiol protease inhibitors, but was not inhibited by metalloprotease or serine protease inhibitors. From the results, it seems likely that these proteases, produced by S. aureus from diseased chickens, might belong to the thiol protease group. 相似文献
89.
90.