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排序方式: 共有164条查询结果,搜索用时 15 毫秒
71.
Hiep Thi Nguyen Thanh Quang Dang-Nguyen Tamas Somfai Nguyen Thi Men Barbara Beck-Woerner Nguyen Viet Linh Bui Xuan Nguyen Junko Noguchi Hiroyuki Kaneko Kazuhiro Kikuchi 《Animal Science Journal》2021,92(1):e13650
Male pronucleus (MPN) formation is a very important physiological event during fertilization, which affects in vitro production of transferrable embryos. The aim of this study was to find out the correlation between the number of penetrated sperm and the occurrence of failure of MPN formation in porcine oocytes. In vitro matured porcine oocytes were fertilized in vitro with frozen epididymal sperm. Two different frozen sperm lots were tested in this study, which were different in terms of polyspermy rates. The numbers and the status of penetrated sperm in oocytes were evaluated 10 h after insemination. Under high polyspermy condition, the polyspermy rate was 83.5% with an average mean of 3.5 sperms per penetrated oocyte, whereas the percentage of polyspermy was 65.5% with an average mean of 2.4 sperms per penetrated oocyte under moderate polyspermic condition. Correlation analysis revealed a negative correlation between the number of penetrated sperm and their MPN formation percentage both in the sperm lot of high polyspermy (R = −0.560, p < 0.05) and in the sperm lot of moderate polyspermy (R = −0.405, p < 0.05) which suggests that penetration of excessive spermatozoa disables the oocyte cytoplasm to promote MPN formation. 相似文献
72.
Sasaki Y Kodama R Iwashige S Fujishima J Yoshikawa T Kamimura Y Maeda H 《Journal of toxicologic pathology》2011,24(1):69-73
Severe bilateral cataract was found in a 7 year-old naïve female cynomolgus monkey
(Macaca fascicularis) 3 months before necropsy. During macroscopic
examination, severe opacity and thinning of the lens were observed in both eyes.
Histopathology revealed that the lens nuclei and majority of cortex lens fibers had
disappeared and become excavated, while the lens fibers in the subcapsular area were
swollen and distorted. Other observations included atrophy and vacuolation in the lens
epithelial cells and proliferation of spindle cells and collagen fiber beneath the
anterior capsule of the right eye. Immunohistochemical staining of these spindle cells
revealed the presence of vimentin, cytokeratin and α-smooth muscle actin (α-SMA), which
were considered to be derived from lens epithelial cells. This is a rare case of
spontaneous, bilateral, hypermature cataract in a cynomolgus monkey. 相似文献
73.
Koji Misumi Yuri Hirayama Misae Suzuki Michiko Nakai Junko Noguchi Hiroyuki Kaneko Kazuhiro Kikuchi 《Animal Science Journal》2014,85(2):112-117
Collection efficacy and in vitro embryo developmental ability of oocytes obtained from Duroc‐breed ovary donors at different stages of the estrous cycle (days 6, 12 and 16 after estrus) were performed. The numbers of collected oocytes did not differ significantly among the different estrous cycle groups (total 72–90 oocytes per gilt). However, the blastocyst rates of oocytes collected on days 12 and 16 (9.2% and 19.4%, respectively) were significantly higher than those on day 6 (1.1%). More oocytes were obtained on day 16 from small follicles (<2 mm in diameter; 85.3 oocytes per gilt) than from medium‐sized (≥2–<6 mm) and large (≥6 mm) follicles (17.5 and 12.8 oocytes, respectively). The blastocyst rates in both the medium‐sized and large follicle groups (20.0% and 19.2%, respectively) were significantly higher than that in the small follicle group (6.3%). The blastocyst cell numbers in both the medium‐sized and large follicle groups (39.4 and 43.3 cells, respectively) were significantly higher than that in the small follicle group (30.6 cells). The results suggest that oocyte collection from cycling Duroc pigs can be carried out efficiently from the late luteal to follicular stage. Those oocytes collected from medium‐sized and large follicles show better embryo development. 相似文献
74.
Satomi Hashimoto Takuya Doi Yumi Wako Junko Sato Sou Wada Minoru Tsuchitani 《Journal of toxicologic pathology》2013,26(3):275-281
We have recently started using Wistar Hannover rats in Japan and are now collecting background data. We have been frequently observing corneal mineralization in Wistar Hannover rats of both the RccHanTM:WIST and Crl:WI (Han) strains. In this study, details of corneal mineralization in Wistar Hannover rats were histopathologically and ultrastructurally investigated. According to the results, Wistar Hannover rats had a much higher incidence of corneal mineralization compared with Sprague-Dawley rats. The incidence of corneal mineralization was higher in males than females. According to the histological examination, mineral deposits were positive for calcium by von Kossa’s method. Furthermore, in response to mineralization, keratocytes probably become active to play an important role against the mineralized substance. 相似文献
75.
76.
Nguyen Viet LINH Kazuhiro KIKUCHI Michiko NAKAI Fuminori TANIHARA Junko NOGUCHI Hiroyuki KANEKO Thanh Quang DANG-NGUYEN Nguyen Thi MEN Nguyen VAN HANH Tamas SOMFAI Bui Xuan NGUYEN Takashi NAGAI Noboru MANABE 《The Journal of reproduction and development》2013,59(6):549-556
Mitochondria are reported to be critical in in vitro maturation of
oocytes and subsequent embryo development after fertilization, but their contribution
for fertilization has not been investigated in detail. In the present study, we
investigate the contribution of mitochondria to fertilization using reconstructed
porcine oocytes by fusion of ooplasmic fragments produced by serial centrifugations
(centri-fusion). Firstly, we evaluated the characteristics of ooplasmic fragments.
Three types of fragments were obtained by centrifugation of porcine oocytes matured
in vitro for 46 h: brownish (B), transparent (T) and large (L)
fragments containing both B and T parts in a fragment. The production efficiencies of
these types of fragments were 71.7, 91.0 and 17.8 fragments/100 oocytes,
respectively. In experiments, L fragments were excluded because they contained both
brownish and transparent components that were apparently intermediate between B and T
fragments. Observations by confocal microscopy after staining with MitoTracker Red
CMXRos® and transmission electron microscopy revealed highly condensed
active mitochondria in B fragments in contrast to T fragments that contained only
sparse organelles. We reconstructed oocytes by fusion of a karyoplast and two
cytoplasts from B and T fragments (B and T oocytes, respectively). The B oocytes
showed higher sperm penetration (95.8%) and male pronuclear formation rates (94.2%)
by in vitro fertilization than T oocytes (66.7% and 50.0%,
respectively). These results suggest that the active mitochondria in oocytes may be
related to their ability for fertilization. 相似文献
77.
78.
Akihiko Kamoshita Hiroyuki Ikeda Junko Yamagishi Bunna Lor Makara Ouk 《Weed Biology and Management》2016,16(3):93-107
The effects of the planting method (transplanting vs. direct seeding), rice cultivar (Sen Pidao vs. Phka Rumduol), and herbicide application (admixture of bentazone and cyhalofop‐butyl) on weeds and weed seed banks were quantified in unflooded, shallowly flooded, and deeply flooded paddy fields in Cambodia in 2005 and 2006. Broad‐leaved weeds infested more toward maturity in 2006 than in 2005, particularly in directly seeded plots. Weed dry weights at pre‐heading and/or maturity were consistently reduced by herbicide application and Phka Rumduol cultivar, while weed numbers increased under unflooded condition. The proportion of sedges was consistently larger in directly seeded and non‐herbicide plots at pre‐heading. Larger numbers and dry weights of sedges and total weeds in 2005 caused larger seed bank sizes of sedges and total weeds in 2006, which further caused their infestation in 2006. A greater weed dry weight at 62 days after sowing in 2005 resulted in larger seed banks of Cyperus iria and Fimbristylis miliacea, which were most severe under non‐herbicide, direct‐seeded treatment, while that at rice maturity resulted in larger seed banks of Cyperus difformis, Scirpus juncoides, and Lindernia antipoda. Overall, sedges shared the majority of the total weed seed bank, followed by broad‐leaved weeds and then grasses. A lower yield in 2005 led to significantly larger seed bank sizes of sedges, but not of grasses or broad‐leaved weeds. Rice yield reduction was consistently related to larger numbers of sedges by heading and those of grasses at maturity. 相似文献
79.
80.
Sawano Shinji Hasegawa Toshihiro Ishigooka Yasushi Kuwagata Tsuneo Shindo Junko Tao Fulu 《Paddy and Water Environment》2015,13(4):529-543
Paddy and Water Environment - Analyses of the technological and climatic factors that influence regional yield can provide insights into how production systems can be improved in the future. We... 相似文献