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401.
Use of Trichoderma spp. for biological control of the livestock feed ontaminant fungus Fusarium proliferatum 下载免费PDF全文
Ruocco M Pane F Ritieni A Lanzuise S Ambrosino P Marra R Woo S L Ciliento R Soriente I Ferraioli S Scala F Lorito M 《浙江大学学报(农业与生命科学版)》2004,30(4):414-414
Fusarium spp. are pathogens of many important agricultural crops, and are often strong mycotoxin producers. Fusarium proliferatum, in particular, causes disease in cereals and secretes the toxin Beauvaricin that contaminates livestock feed and cereals, producing a variety of toxicity symptoms ranging from poor weight gain to mortality. Beauvaricin is a cyclodepsipeptide and acts as a potent mycotoxin known to have insecticidal properties. This compound is highly toxic to human cell lines, wh… 相似文献
402.
The Trichoderma-plant interaction is mediated by avirulence proteins produced by this fungus 下载免费PDF全文
Ruocco M Lanzuise S Woo S L Ambrosino P Marra R Turrà D Gigante S Formisno E Scala F Kip N P J G M de Wit Lorito M 《浙江大学学报(农业与生命科学版)》2004,30(4):422-422
The molecular basis of Trichoderma -plant interaction is very complex and still not completely understood. The colonization of the root system by rhizosphere competent strains of Trichoderma results in increased development of root/aerial systems, in improved yields and in plant disease control. Other beneficial effects, such as the induction of plant systemic resistance, have also been described. To understand the mechanisms involved we are using different approaches, including the making… 相似文献
403.
Effect of rare earth application on the growth of Trichoderma spp. and several plant pathogenic fungi 下载免费PDF全文
d''Aquino L Carboni M Woo S L Morgana M Nardi L Lorito M 《浙江大学学报(农业与生命科学版)》2004,30(4):424-424
Rare earth elements (REEs) enriched fertilisers are currently used in China for soil and foliar applications to crops, but little is known about the effect of REEs applications on the growth of beneficial and detrimental soilborne microorganisms. The growth of biological control agents Trichoderma atroviride strain P1, Trichoderma harzianum strain A6 and strain T22, plant pathogens Botrytis cinerea, Alternaria alternata, Fusarium solani, Rhizoctonia solani and Sclerotinia scleroti… 相似文献
404.
Hwang WS Roh SI Lee BC Kang SK Kwon DK Kim S Kim SJ Park SW Kwon HS Lee CK Lee JB Kim JM Ahn C Paek SH Chang SS Koo JJ Yoon HS Hwang JH Hwang YY Park YS Oh SK Kim HS Park JH Moon SY Schatten G 《Science (New York, N.Y.)》2005,308(5729):1777-1783
Patient-specific, immune-matched human embryonic stem cells (hESCs) are anticipated to be of great biomedical importance for studies of disease and development and to advance clinical deliberations regarding stem cell transplantation. Eleven hESC lines were established by somatic cell nuclear transfer (SCNT) of skin cells from patients with disease or injury into donated oocytes. These lines, nuclear transfer (NT)-hESCs, grown on human feeders from the same NT donor or from genetically unrelated individuals, were established at high rates, regardless of NT donor sex or age. NT-hESCs were pluripotent, chromosomally normal, and matched the NT patient's DNA. The major histocompatibility complex identity of each NT-hESC when compared to the patient's own showed immunological compatibility, which is important for eventual transplantation. With the generation of these NT-hESCs, evaluations of genetic and epigenetic stability can be made. Additional work remains to be done regarding the development of reliable directed differentiation and the elimination of remaining animal components. Before clinical use of these cells can occur, preclinical evidence is required to prove that transplantation of differentiated NT-hESCs can be safe, effective, and tolerated. 相似文献
405.
Woo HA Chae HZ Hwang SC Yang KS Kang SW Kim K Rhee SG 《Science (New York, N.Y.)》2003,300(5619):653-656
The active-site cysteine of peroxiredoxins is selectively oxidized to cysteine sulfinic acid during catalysis, which leads to inactivation of peroxidase activity. This oxidation was thought to be irreversible. However, by metabolic labeling of mammalian cells with 35S, we show that the sulfinic form of peroxiredoxin I, produced during the exposure of cells to H2O2, is rapidly reduced to the catalytically active thiol form. The mammalian cells' ability to reduce protein sulfinic acid might serve as a mechanism to repair oxidatively damaged proteins or represent a new type of cyclic modification by which the function of various proteins is regulated. 相似文献
406.
Bargheer M Zhavoronkov N Gritsai Y Woo JC Kim DS Woerner M Elsaesser T 《Science (New York, N.Y.)》2004,306(5702):1771-1773
Reversible structural changes of a nanostructure were measured nondestructively with subpicometer spatial and subpicosecond temporal resolution via x-ray diffraction (XRD). The spatially periodic femtosecond excitation of a gallium arsenide/aluminum gallium arsenide superlattice results in coherent lattice motions with a 3.5-picosecond period, which was directly monitored by femtosecond x-ray pulses at a 1-kilohertz repetition rate. Small changes (DeltaR/R = 0.01) of weak Bragg reflexes (R = 0.005) were detected. The phase and amplitude of the oscillatory XRD signal around a new equilibrium demonstrate that displacive excitation of the zone-folded acoustic phonons is the dominant mechanism for strong excitation. 相似文献
407.
Rivera MN Kim WJ Wells J Driscoll DR Brannigan BW Han M Kim JC Feinberg AP Gerald WL Vargas SO Chin L Iafrate AJ Bell DW Haber DA 《Science (New York, N.Y.)》2007,315(5812):642-645
Wilms tumor is a pediatric kidney cancer associated with inactivation of the WT1 tumor-suppressor gene in 5 to 10% of cases. Using a high-resolution screen for DNA copy-number alterations in Wilms tumor, we identified somatic deletions targeting a previously uncharacterized gene on the X chromosome. This gene, which we call WTX, is inactivated in approximately one-third of Wilms tumors (15 of 51 tumors). Tumors with mutations in WTX lack WT1 mutations, and both genes share a restricted temporal and spatial expression pattern in normal renal precursors. In contrast to biallelic inactivation of autosomal tumor-suppressor genes, WTX is inactivated by a monoallelic "single-hit" event targeting the single X chromosome in tumors from males and the active X chromosome in tumors from females. 相似文献
408.
Chan Woo Lee 《Fibers and Polymers》2007,8(6):571-578
This study describes the synthesis of D-lactic acid from rice by fermentation using microorganisms. Some micro-organisms were
found to be active for producing D-lactic acid of high optical purity after an intensive screening test for D-lactic acid
bacteria using glucose as substrate. Rice powder was hydrolyzed with a combination of enzymes:α-amylase,β-amylase, and pullulanase, and the resultant rice saccharificate was subjected to the fermentation with the selected D-lactic
acid bacteria. After the optimization of this fermentation it has been confirmed that D-lactic acid can be manufactured in
a pilot scale. 相似文献
409.
Sung Eun Kim Yong Woo Cho Eun Jung Kang Ick Chan Kwon Eunhee Bae Lee Jung Hyun Kim Hesson Chung Seo Young Jeong 《Fibers and Polymers》2001,2(2):64-70
A three-dimensional, porous collagen/chitosan complex sponge was prepared to closely simulate basic extracellular matrix (ECM)
constitutes, collagen and glycosaminoglycan. The complex sponge was prepared by a lyophilization method and had the regular
network with highly porous structure, suitable for cell adhesion and growth. The pores were well interconnected, and their
distribution was fairly homogeneous. The complex sponge was crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide
(EDC) and N-hydroxysuccinimide (NHS) to increase its biological stability and enhance its mechanical properties. The crosslinking
medium had a great effect on the inner structure of the sponge. The homogeneous, porous structure of the sponge was remarkably
collapsed in an aqueous crosslinking medium. However, the morphology of the sponge remained almost intact in a water/ethanol
mixture crosslinking milieu. Mechanical properties of the collagen/chitosan sponge were significantly enhanced by EDC-mediated
crosslinking. The potential of the sponge as a scaffold for tissue engineering was investigated using a Chinese hamster ovary
cell (CHO-K1) line. 相似文献
410.
Kwan Woo Kim Keun Hyung Lee Bong Seok Lee Yo Seung Ho Seung Jin Oh Hak Yong Kim 《Fibers and Polymers》2005,6(2):121-126
Semicrystalline poly(ethylene terephthalate) (cPET)/amorphous poly(ethylene terephthalate) with isophthalic acid (aPET) blends
with 100/0, 75/25, 50/50, 25/75, and 0/100 by weight ratios were dissolved in a mixture of trifluoroacetic acid (TFA)/methylene
chloride (MC) (50/50, v/v) and electrospun via the electrospinning technique. Solution properties such as solution viscosity,
surface tension and electric conductivity were determined. The solution viscosity slightly decreased as aPET content increased,
while there was no difference in surface tension with respect to aPET composition. The characteristics of the electrospun
cPET/aPET blend nonwovens were investigated in terms of their morphology, pore size and gas permeability. All these measurements
were carried out before and after heat treatment for various blend weight ratios. The average diameter of the fibers decreased
with increasing aPET composition due to the decrease in viscosity. Also, the morphology of the electrospun cPET/aPET blend
nonwovens was changed by heat treatment. The pore size and pore size distribution varied greatly from a few nanometers to
a few microns. The gas permeability after heat treatment was lower than that before heat treatment because of the change of
the morphology. 相似文献