Production and characterization of an alloplasmic and monosomic addition line of Brassica rapa carrying the cytoplasm and one chromosome of Moricandia arvensis

Intergeneric hybridization was performed between Moricandia arvensis and four inbred lines of Brassica rapa following embryo rescue. Three F₁ hybrid plants were developed from three cross combinations of M. arvensis × B. rapa, and amphidiploids were synthesized by colchicine treatment. Six BC₁ plants were generated from a single cross combination of amphidipolid × B. rapa ‘Ko1-303’ through embryo rescue. One BC₂ and three BC₃ plants were obtained from successive backcrossing with B. rapa ‘Ko1-303’ employing embryo rescue. Alloplasmic and monosomic addition lines of B. rapa (Allo-MALs, 2n = 21) were obtained from backcrossed progeny of three BC₃ plants (2n = 21, 22 and 23) without embryo rescue. An alloplasmic line of B. rapa (2n = 20) degenerated before floliation on 1/2 MS medium due to severe chlorosis. Allo-MALs of B. rapa (2n = 21) showed stable male sterility without any abnormal traits in vegetative growth and female fertility. Molecular analyses revealed that the same chromosome and cytoplasm of M. arvensis had been added to each Allo-MAL of B. rapa. This Allo-MAL of B. rapa may be useful material for producing cytoplasmic male sterile lines of B. rapa.     

Genetically diverse group C rotaviruses cause sporadic infection in Korean calves

This study examined the prevalence and genetic diversity of the bovine group C rotaviruses (GCRVs) in a total of 127 diarrhea fecal samples of calves from 52 Korean native beef calf herds using RT-PCR and nested PCR. Overall, seven of the 127 fecal samples (5.5%) from seven of the 52 herds (13.5%) tested positive for bovine GCRVs only by nested PCR. Sequence and phylogenetic analyses of a partial VP6 gene showed that Korean bovine GCRVs had marked genetic diversity; two Korean strains belonged to the bovine lineage, whereas five Korean strains belonged to the porcine lineage. These results suggest that the genetically diverse bovine GCRVs cause sporadic infections in diarrheic calves in South Korea.     

对妊娠母猪应用的理想蛋白质模式及其对氨基酸的需要量

由于蛋白质摄入量受限而用于胎儿组织（McPherson等,2004）和乳腺实质组织（Kim等,1999;Ji等,2006）生长以及猪乳合成（Revell等,1998;Jones和Stahly,1999）所需的营养增加,导致妊娠后期和哺乳阶段的母猪处于营养分解状态。母体处于分解状态对胎儿和新生仔猪的生长不利,也增加了它们的患病率和死亡率（Wu等,2006）。在蛋白质摄入量受限的情况下,尤其是在妊娠期和哺乳期,向母猪提供获得最大利用效率的理想平衡氨基酸是重要的。     

Real time observation of mouse fetal skeleton using a high resolution X-ray synchrotron

The X-ray synchrotron is quite different from conventional radiation sources. This technique may expand the capabilities of conventional radiology and be applied in novel manners for special cases. To evaluate the usefulness of X-ray synchrotron radiation systems for real time observations, mouse fetal skeleton development was monitored with a high resolution X-ray synchrotron. A non-monochromatized X-ray synchrotron (white beam, 5C1 beamline) was employed to observe the skeleton of mice under anesthesia at embryonic day (E)12, E14, E15, and E18. At the same time, conventional radiography and mammography were used to compare with X-ray synchrotron. After synchrotron radiation, each mouse was sacrificed and stained with Alizarin red S and Alcian blue to observe bony structures. Synchrotron radiation enabled us to view the mouse fetal skeleton beginning at gestation. Synchrotron radiation systems facilitate real time observations of the fetal skeleton with greater accuracy and magnification compared to mammography and conventional radiography. Our results show that X-ray synchrotron systems can be used to observe the fine structures of internal organs at high magnification.     

A high fiber diet responsive case in a poodle dog with long-term plant eating behavior

An 11-year-old, castrated male Miniature Poodle dog was referred due to daily plant eating followed by vomiting for 7 years. Each time the dog went out for a walk, he had been munched grass, and then vomited. There were no abnormal findings on medical examinations. Through behavior examination and consultation, we diagnosed this dog as having a plant eating problem. Because we assumed that the dog had eaten plants to supply of dietary deficiency or relieve digestive problems, advised the owner to discontinue supplying previous diet and to feed high-fiber diet. On the follow up, the owner said that the dog had not eaten plants and vomited from 3 days after initial dietary change. The dog has not shown any clinical signs for 13 months. This clinical finding shows a possibility that deficient diet, especially fiber, relates to the plant eating behavior of a dog.     

Evaluation of immunochromatographic assay for serodiagnosis of Brucella canis

Canine brucellosis is a contagious disease with venereal and oral modes of transmission that produces late abortion in females, epididymides and prostates in males. Diagnosis is difficult because of unstable serum antibody titers that vary from individual to individual as well as between different methods used for their detection. The objective of this work was to evaluate the clinical utility of the immunochromatographic assay (ICA) for serodiagnosis of dogs suspected of having brucellosis, and results were compared with those obtained for hemoculture (HC) and the rapid screening agglutination with 2-mercaptoethanol (2-ME RSAT). The all experimentally infected dogs were positive in ICA, HC and 2-ME RSAT from 5 weeks, 7 weeks, and 3 weeks after infection, respectively. Also, among dogs selected from 10 different breed kennels occurred brucellosis, 24.8%, 39.5% and 39.1% of dogs tested were detected as positive with HC, 2-ME RSAT and ICA, respectively. The kappa value between 2-ME RSAT and ICA was 0.89. The results of this study showed that sensitivity and specificity of the ICA are comparable with those obtained using conventional serological and bacteriological test for brucellosis. In conclusion, the ICA kit provides a handy and accurate tool for the rapid serodiagnosis of canine brucellosis.     

Far infrared irradiation alters total polyphenol,total flavonoid,antioxidant property and quercetin production in tartary buckwheat sprout powder

The effects of far infrared irradiation (FIR) on total polyphenol (TP), total flavonoid (TF) content, antioxidant properties and aglycone quercetin production in tartary buckwheat sprout (TBS) were investigated. The study showed that FIR treatment decreased the total antioxidant capacity and metal chelating property in TBS in a temperature dependent manner, however, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity increased with the temperature. Similarly, TP and TF content also increased with temperature. The HPLC result revealed that quercetin production was directly proportional to the temperature, and the maximum production (average 14.8 mg/g dw) of quercetin was at 120 °C which was 13.5 times higher than the untreated control sample of TBS. Overall, this research is expected to be helpful to improve the nutritional value of tartary buckwheat by optimizing the FIR conditions.     

Preparation of poly(vinyl alcohol)/silver-zeolite composite hydrogels by UV-irradiation

Poly(vinyl alcohol) (PVA)/Ag-zeolite nanocomposite hydrogels were prepared by UV irradiation using PVA solution mixed with Ag-zeolite nanoparticles. Physical properties and changes in morphology of the PVA/Ag-zeolite hydrogels were investigated. The PVA/Ag-zeolite hydrogels were prepared at a PVA concentration of 9 wt% with a UV irradiation distance of 15 cm, where gel fraction and swelling ratio were optimized. Hardness of the PVA/Ag-zeolite hydrogels decreased with increasing amounts of Ag-zeolite, reaching that of soft elastomer when the amount of Ag-zeolite was 5 % by weight. The PVA/Ag-zeolite hydrogels showed strong antimicrobial activities against Staphylococcus aureus and Klebsiella pneumoniae, inducing a reduction of bacteria of over 99.9 % at a Ag-zeolite content of 3 wt%.     

Contributing ratio of the mechanical and the physicochemical actions to washing efficiency of mineral oil and protein soils

It was attempted to separate the detergency contributions from the mechanical actions and the physicochemical actions for two different types of soils. The mechanical washing efficiency was evaluated through the washing performed under the mechanical agitation, in the absence of detergent. The physicochemical washing efficiency was examined in the washing bath with detergent in the absence of mechanical agitation. Among the washing parameters, extended washing time and higher washing temperature were effective improving the mechanical and physicochemical washing efficiencies for mineral oil and carbon black. For blood soil, lower washing temperature and extended washing time enhanced the mechanical and physicochemical washing efficiencies. For the mineral oil, the presence of both mechanical and physicochemical actions led to the effective washing performance with synergistic effect. For the blood soil, mechanical actions were observed as more contributing than the physicochemical actions. The optimized washing program could be recommended as per the contributing ratios for the specific soil types, minimizing the energy consumption and detergent usage while achieving the washing performance.