ABA、H_2O_2与NO供体硝普钠对基因表达以及枣果发育的影响

枣在成熟期间遇到连阴雨天气会发生严重的裂果现象,这种基于基因表达的生理病害的发生与非生物或生物胁迫有关。以吕梁当地木枣为试材,在木枣成熟期,采用改良CTAB法检测组织中DNA纯度及含量的变化,研究ABA、H2O2与NO供体硝普钠(SNP)在枣果发育中对基因表达的影响。结果表明:ABA、H2O2与NO供体硝普纳(SNP)对枣果发育中DNA纯度及含量的影响不一,差异较为明显。其中不同浓度的ABA处理枣果组织中DNA的OD260/OD280比值基本不在1.700~1.900范围内,DNA纯度差异明显。但样品DNA含量较高,其值在1 200ng/μL左右;H2O2和SNP处理样品DNA的OD260/OD280比值基本上在1.700~1.900之间,DNA纯度较高。样品DNA含量基本上小于或在1 000ng/μL左右。外源ABA较为明显地干扰了基因表达,诱导了新蛋白质、RNA和酚类物质等的生物合成,有促进木枣果发育的趋势;而H2O2和NO供体硝普钠(SNP)对基因表达或枣果发育的影响不十分明显。     

Impact of fluxapyroxad on the microbial community structure and functional diversity in the silty-loam soil

The aim of this work was to assess the effect of applying three different doses of fluxapyroxad on microbial activity, community structure and functional diversity as measured by respiration, microbial biomass C, phospholipid fatty acid(PLFA) and community-level physiological profiles(CLPPs). Our results demonstrated that substrate-induced respiration(on day 15) and microbial biomass C(on days 7 and 15) were inhibited by fluxapyroxad, but stimulation was observed thereafter. In contrast, fluxapyroxad addition increased the basal respiration and metabolic quotients(q CO2) and respiratory quotients(QR). Analysis of the PLFA profiles revealed that the total and bacterial biomass(both Gram-positive bacteria(GP) and Gram-negative bacteria(GN)) were decreased within the initial 15 days, whereas those as well as the GN/GP ratio were increased at days 30 and 60. F luxapyroxad input decreased the fungi biomass but increased the bacteria/fungi ratio at all incubation time. Moreover, high fluxapyroxad input(75 mg fluxapyroxad kg–1 soil dry weight) increased the microbial stress level. A principal component analysis(PCA) of the PLFAs revealed that fluxapyroxad treatment s ignificantly shifted the microbial community structure, but all of the observed effects were transient. Biolog results showed that average well color development(AWCD) and functional diversity index(H′) were increased only on day 60. I n addition, the dissipation of fluxapyroxad was slow in soil, and the degradation half-lives varied from 158 to 385 days depending on the concentration tested.     

园林机械（以割草机为代表）用改性PP材料开发与应用

文章从PP材料的改性原理、园林机械用改性PP材料的性能要求出发,全面阐述了园林机械用改性PP材料的开发研制过程,开发出了一系列园林机械用改性PP材料并全面在ZS系列园林机械上得到了应用。     

立式无筛饲料超微粉碎机的研发及质量检测分析

介绍了我国立式无筛饲料超微粉碎机的发展,简述立式无筛饲料超微粉碎机的组成、工作原理和优越性,分析质量检测及立式无筛饲料超微粉碎机技术的发展,展望制定行业标准的预期效果。     

房山区中南部沟域景观资源分析及规划探讨

本文从风景园林规划的基本目的入手,通过实践和调研房山区中南部沟域景观资源,分析地区内各景观体系类型,分类整合地区景观资源特征,挖掘房山中南部景观资源本身价值,并通过对房山区中南部地区进行上方花海沟域景观规划,系统研究房山区中南部沟域地区的景观建设发展方向,为深层综合开发、培育和保护当地景观资源提出改进措施,亦为房山区中南部的沟域经济的建设发展提供必要的理论依据。     

产甲壳素酶菌株的筛选与诱变

以胶体甲壳素作为惟一碳源,从自然界微生物中筛选并诱导到一株产甲壳素酶活性较高的菌株,经16S r DNA鉴定为Aeromonas sp.。并以该菌株作为出发菌株,经过一次紫外诱变,酶活是初筛菌株的1.48倍。再经过二次紫外诱变或者紫外-Li Cl复合诱变,酶活分别是初筛菌株的3.16和3.87倍,最高酶活达到3.48 U/m L。最后通过连续传代培养,确定了菌株HMX-16经过紫外和复合诱变后突变性状稳定性良好。     

Effects of Addition of Three Types of Biochar on the Soil Nitrogen Balance and Cotton Yield

The objective of this study was to determine the effect of biochar addition on the soil N balance and cotton yield in a drip-irrigated cotton field. The experiment included four soil amendments: control, cotton straw biochar, corn cob biochar, and poultry manure biochar. The biochar was applied at a rate of 4.5 t·hm^－2 except control. The results showed that all three types of biochar significantly reduced both the apparent soil N loss and the N surplus. The effect of cotton straw biochar treatment was most significant, relative to the control. Plant nitrogen uptake differed significantly between the different treatments, decreasing in the order cotton straw biochar > poultry manure biochar > corn cob biochar. Cotton yield also differed significantly between the different treatments, decreasing in the order poultry manure biochar > corn cob biochar, and cotton straw biochar.     

废名新诗理论与中国“自然”诗学传统

传统的经典理论分析了政府供给公共产品和消费者无差别消费时的经济问题。然而在市场经济中消费者的类型是不相同的，对公共产品的消费需求也各不相同。对此，需要研究不同消费需求、不同供给模式下具有“消费信号”的公共产品自愿供给问题。研究发现，提高公共产品消费的信号性，优化公共产品的供给模式对于公共产品的自愿供给非常重要。同时，识别出“信号消费”下的异质消费者，并使公共产品具有私人产品的消费特征时，公共产品自愿供给的意愿将会增加。     

淮安区水稻测土配方施肥配方校正与示范对比试验报告

为验证肥料配方的准确性,发挥配方肥料施用的最大效益。通过田间校验,示范对比测土配方施肥的增产效果,进一步验证并完善淮安区的肥料配方,优化测土配方施肥技术参数。     

Effects of bFGF on brain edema,nerve function injury and autophagy related protein in rats with traumatic brain injury

AIM:To study the effects of basic fibroblast growth factor (bFGF) on brain edema, nerve function damage and autophagy related proteins in rats with head injury. METHODS:The rat model of craniocerebral injury (CI) was constructed. The rats were divided into control group, CI group, and low-, middle-and high-dose bFGF groups (n=10). The CI model was established in CI group, while the rats in control group were not given epidural impact. The rats in low-dose, middle-dose and high-dose bFGF groups were given bFGF at 2, 4 and 6 μg, respectively, by intraperitoneal injection after 30 min. The neurological function in the rats was evaluated by improved neurological function scoring. The rat brain tissues were taken, and the water content was detected. The levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1β in the brain tissue were measured by ELISA. The malondialdehyde (MDA) content was analyzed by thiobarbituric acid method. The activity of superoxide dismutase (SOD) was examined by WST-8 assay. The glutathine peroxidase (GSH-Px) activity was detected by colorimetric method. The protein levels of autophagy related proteins LC3-Ⅱ and beclin-1 in the brain tissues were determined by Western blot. RESULTS:The neurological function score was increased significantly of the rats in CI group. The rat model of craniocerebral injury was successfully constructed. Neurological function scores in the rats in low-dose, middle-dose and high-dose bFGF groups were reduced, the water content of the brain tissue was also reduced (P<0.05). The levels of TNF-α, IL-6 and IL-1 β were decreased in the brain tissues (P<0.05), the content of MDA was declined (P<0.05), the activities of SOD and GSH-Px were increased (P<0.05), the protein levels of LC3-Ⅱ and beclin-1 were decreased, compared with the untreated rats in CI group (P<0.05). CONCLUSION:bFGF improves the nerve function of the rats with craniocerebral injury, reduces the water content of the brain tissue, reduces the expression of autophagic protein LC3-Ⅱ and beclin-1.The mechanism is related to the inhibition of inflammatory reaction and oxidative damage.