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排序方式: 共有286条查询结果,搜索用时 15 毫秒
101.
Pejchal R Doores KJ Walker LM Khayat R Huang PS Wang SK Stanfield RL Julien JP Ramos A Crispin M Depetris R Katpally U Marozsan A Cupo A Maloveste S Liu Y McBride R Ito Y Sanders RW Ogohara C Paulson JC Feizi T Scanlan CN Wong CH Moore JP Olson WC Ward AB Poignard P Schief WR Burton DR Wilson IA 《Science (New York, N.Y.)》2011,334(6059):1097-1103
The HIV envelope (Env) protein gp120 is protected from antibody recognition by a dense glycan shield. However, several of the recently identified PGT broadly neutralizing antibodies appear to interact directly with the HIV glycan coat. Crystal structures of antigen-binding fragments (Fabs) PGT 127 and 128 with Man(9) at 1.65 and 1.29 angstrom resolution, respectively, and glycan binding data delineate a specific high mannose-binding site. Fab PGT 128 complexed with a fully glycosylated gp120 outer domain at 3.25 angstroms reveals that the antibody penetrates the glycan shield and recognizes two conserved glycans as well as a short β-strand segment of the gp120 V3 loop, accounting for its high binding affinity and broad specificity. Furthermore, our data suggest that the high neutralization potency of PGT 127 and 128 immunoglobulin Gs may be mediated by cross-linking Env trimers on the viral surface. 相似文献
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Tendro Radanielina Alain Ramanantsoanirina Louis-Marie Raboin Julien Frouin Xavier Perrier Philippe Brabant Nourollah Ahmadi 《Genetic Resources and Crop Evolution》2013,60(1):311-323
In situ conservation of crop genetic resources is widely recommended but, as yet, no methods have been developed to rank the genetic entities to preserve and the social organisations to involve. The highlands of Madagascar have been identified as a key site for rice, Oryza sativa, genetic diversity. To define conservation strategies, we performed multidisciplinary analysis of rice genetic diversity and factors shaping its distribution in the target region. Along with the indica and japonica rice subspecies of O. sativa, we confirmed the presence of an atypical rice group with a preferential habitat of 1,250–1,750 m. Spatial distribution of genetic diversity was uneven. The most determining factor of this unevenness was the altitude authorising or not the presence of different rice cropping systems and the associated types of varieties. Village and individual farmer’s wealth also had a determining role on the amount of rice diversity they hosted. While molecular variance between villages in a given interval of altitude represented 16 % of the total variance, within-village variance represented more than 75 % of the total, and within-farm variance 70 % of within-village variance. This hierarchical distribution of molecular variance suggests that a small number of samples per scale (altitude interval, village and farm) could allow to capture most of the genetic diversity observed. However, within-variety diversity was also important making ex situ conservation strategies impractical and costly. Implications of the within-variety diversity are discussed in terms of adaptive advantages, evolutionary processes, and need for in situ conservation. 相似文献
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Claire Billy François Birgand Patrick Ansart Julien Peschard Mathieu Sebilo Julien Tournebize 《Landscape Ecology》2013,28(4):665-684
Land managers need to clearly identify the main natural factors controlling nitrate attenuation from upstream to downstream in agricultural watersheds. All interfaces between surface waters and groundwaters such as riparian zones could be identified as retention zones in the watershed. However, in highly human-influenced agricultural landscapes, retention zones could be shortcut, abandoned and sometimes erased. Starting from this situation, this paper aims to underline the role of hydrological and biogeochemical retention processes in the determination of nitrate concentration in an artificially drained agricultural watershed. The Orgeval watershed (East part of Paris, France, belonging to a long-term environmental observatory network) is 80 % covered by drained agricultural plots, 17 % forest and 3 % urban areas and roads, covering a surface area of 104 km2. The watershed is split into several nested sub-basins from 1 to 100 km2. Two levels of monitoring were carried out in the 2007–2008 hydrological year: long-term at six monitoring stations (measuring nitrate concentration and discharge) and 20 points throughout the watershed (measuring discharge, chloride, nitrate, sulphate, calcium concentration and nitrogen isotopic composition) for three different dates (10/18/2007, 01/23/2008, 04/10/2008). Artificial drainage generates modified water transfer and thus nitrate transformation processes during the wet drainage season in winter. Dilution processes provided by forested areas seem to be one of the main factor determining global water quality. A threshold of 34 % forested cover maintains the nitrate concentration below the drinking-water limit (11.3 mgN L?1). Nevertheless, statistical analysis, isotopic measurements and the analysis of the nitrate versus chloride ratio showed that retention processes also influence water quality during the dry season. 相似文献
106.
Oldrich Navratil Olivier Evrard Michel Esteves Sophie Ayrault Irène Lefèvre Cédric Legout Jean-Louis Reyss Nicolas Gratiot Julien Nemery Nicolle Mathys Alain Poirel Philippe Bonté 《Journal of Soils and Sediments》2012,12(9):1463-1478
Purpose
Quantifying suspended sediment fluxes and dynamics across mountains, and identifying the origin of sediment in severely eroded areas, are of primary importance for the management of water resources. This contribution aims to generalise previous results from suspended sediment fingerprinting obtained during 2007?C2009 in a mesoscale Alpine catchment (the Bléone River; 905?km2) in France, and to assess variability in sediment sources throughout the second half of the twentieth century.Materials and methods
Sediment fingerprinting, based on elemental geochemistry and radionuclide measurements, was conducted on a sediment core collected in an alluvial floodplain at the basin outlet. This technique was combined with hydro-sedimentary time-series to reconstruct the origin of suspended sediment deposited at this location over the last 50?years.Results and discussion
Interpretation of sedimentation based on historical hydrological databases corroborates core dating obtained with 137Cs and 210Pbxs activity measurements. Black marls and (marly) limestone sources provided the main fraction of sediment throughout the sequence (40 and 22?%, respectively). However, we also found evidence for the occurrence of major floods carrying large quantities of sediment originating from Quaternary deposits and conglomerates (25 and 16?%, respectively). The variability of sediment sources throughout the sequence may reflect the spatial variability of rainfall within the catchment, which in turn reflects its origin. However, the relatively homogeneous sediment composition throughout the sequence confirms that core-derived information is representative of widespread flood events.Conclusions
These results are consistent with those obtained in previous studies. They also outline the need to take into account the entire grain size range of fine sediment in order to provide an overall picture of sediment sources and transfers within highly erosive catchments. This study also emphasizes the importance of using archival data to validate the results of sediment fingerprinting studies conducted during short contemporary monitoring programmes, and to extend fingerprinting of sediment sources over longer time-scales which include large and widespread floods. 相似文献107.
Biodiversity conservation does predominantly focus on protected natural areas, but has to consider also the usually Human-dominated matrix in which these natural areas are embedded. Here we study highway stormwater retention ponds, which may act as refuges for native flora and fauna and contribute to the maintenance of biodiversity in Human-dominated landscapes. However, the biodiversity supported by such artificial ponds has received little attention so far. Using standardised methods, we addressed the potential role of highway stormwater ponds as refuges by comparing aquatic macroinvertebrate communities (Coleoptera, Heteroptera, Odonata and Gastropoda) in highway stormwater ponds with ponds in the wider landscape. As expected from their pollutant retention function, highway ponds differed in abiotic conditions from surrounding ponds. However, they supported aquatic macroinvertebrate communities at least as rich and diverse at the family level as surrounding ponds and exhibited similar variability in family community composition and structure. The main difference we observed was a higher abundance of small and/or short-lived invertebrates in the highway ponds. These similar community compositions and structures suggest that highway ponds contribute to the biodiversity of the pond network at a regional scale. Thus, road practitioners should consider highway ponds not only for their hydrological and pollutant retaining purposes but also as a possibility to increase the role of highway verges as a refuge and, consequently, landscape connectivity. The management of these water bodies should recognise their potential for biodiversity especially in Human-dominated landscapes. 相似文献
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Alexandru Tutunaru Julien Dupont Vincent Huberty Mostafa Ibrahim Didier Serteyn Charlotte Sandersen 《Veterinary anaesthesia and analgesia》2017,44(4):910-914