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排序方式: 共有470条查询结果,搜索用时 15 毫秒
61.
62.
Schmidt V Schlömer J Lüken C Johne R Biere B Müller H Krautwald-Junghanns ME 《Avian diseases》2008,52(3):380-386
Pigeon circovirus (PiCV) infection and young pigeon disease syndrome (YPDS), associated with high morbidity and mortality, have been recognized in young racing pigeons from large portions of Central Europe. There exist a number of data indicating that YPDS is a consequence of PiCV infection and subsequent immunosuppression. In order to prove PiCV to be one of the crucial factors of YPDS, an experimental infection with PiCV was performed under controlled conditions. Twenty-four domestic pigeons (Columba livia forma domestica) were divided into two groups with 12 pigeons each; an infection group and a control group. All birds were between their fourth to eighth week of life. Pigeons in the infection group were infected both intramuscularly and orally with PiCV purified from naturally infected birds, while pigeons in the control group received a placebo. To test a possible influence of the PiCV infection on the immune system, the animals in both groups were vaccinated simultaneously, on the same day, against PMV-1 (Lasovac plus, IDT, Dessau-Tornau, Germany). Weekly virologic testing showed a viraemic period, and excretion of the infection virus, in pigeons in the infection group. Replication of PiCV could be proved on the basis of histologic findings of multiglobular inclusion bodies, mainly observed in macrophages of the bursa of Fabricius. A PiCV, genetically distinct from the experimental virus, was detected in the control group by polymerase chain reaction (PCR) testing, but any histologic findings comparable to the infection group were absent. None of the pigeons revealed clinical signs of illness, or hints that immunosuppression had occurred, regardless of their group. The absence of stressful conditions, considered as a trigger for the development of YPDS, may be responsible for the failure of disease reproduction in our infection model. 相似文献
63.
为进一步明确麦二叉蚜Schizaphis graminum唾液蛋白Sg1655在调控小麦防御反应中的作用,基于麦二叉蚜唾液腺转录组数据,利用PCR技术克隆获取Sg1655开放阅读框,利用生物信息学软件对其序列进行分析;通过实时荧光定量PCR(real-time quantitative PCR,RT-qPCR)技术检测Sg1655在麦二叉蚜不同组织及不同龄期中的表达;利用细菌III型分泌系统将Sg1655导入到小麦叶片内,检测其瞬时表达对小麦胼胝质积累的影响。结果表明,Sg1655开放阅读框全长375 bp,编码125个氨基酸残基,N端1~21位为信号肽序列,预测蛋白分子量为14.90 kD。麦二叉蚜Sg1655氨基酸序列与玉米蚜Rhopalosiphum maidis同源序列相致性最高,为89.52%;麦二叉蚜Sg1655与玉米蚜同源序列聚为一个分支,亲缘关系最近。Sg1655在麦二叉蚜各组织中均有表达,其中在唾液腺中表达量最高,Sg1655在麦二叉蚜各个龄期均有表达,且在不同龄期之间无显著差异。小麦叶片内Sg1655瞬时表达可显著抑制小麦胼胝质积累,表明该蛋白可作为潜在效应子参与抑制小麦防御反应。 相似文献
64.
Planting trees in tropical countries is an increasingly important forestry activity. Although much attention has been paid, and rightly so, to rapid deforestation in the tropics, the contribution and role that planted forests can play are now at the center of policy and planning in most national forestry strategies. The changing emphasis from exploitative management of natural forests to managed natural forests and plantation forests, seen in temperate regions over the last 100 years, has been taking place in tropical countries largely over the last 20 years. In neither temperate nor tropical regions has this evolution finished.Planted forests will increasingly furnish wood resources that meet domestic wood needs and provide many environmental services. In this paper I seek to place in perspective the contribution that planted forests will make in the tropics. I emphasize (1) the wide variety of purposes that planted forests serve; (2) the sustainability of plantation silviculture; and (3) the overriding importance of due regard to people's participation. I am optimistic about the great value that planted forests, rightly used, can provide for a great many hard-pressed peoples across the tropics and beyond. 相似文献
65.
66.
Bocar Sabaly Bald Julian Dring Werner Ekau Malick Diouf Patrice Brehmer 《Fisheries Oceanography》2019,28(6):686-697
Successful recruitment in small pelagic fish populations inhabiting upwelling zones is subject to variation in fecundity and is driven by spatial and temporal fluctuations in environmental conditions, that is, mainly sea surface temperature, salinity and food availability. These fluctuations in abiotic factors have stimulated small pelagic fish populations to exhibit specifically adapted spawning tactics. To better understand to what extent a short‐lived exploited fish species such as bonga shad Ethmalosa fimbriata has adapted to an upwelling environment, we have investigated the interrelationship between upwelling intensity as a proxy for productivity and population fecundity by means of a virtual population analysis. We found that females of intermediate size contributed significantly more eggs to the population's fecundity than smaller or larger ones. Our model results further indicate that E. fimbriata exhibits a spawning preference at water temperatures of around 25°C and upwelling intensities of around 2.5 m3 s?1 m?1. Hence, we hypothesize that climate change‐driven increases in sea temperatures and modifications of upwelling‐favourable winds could significantly impact the species’ reproductive biology. To understand how climate change might impact fisheries, spawning tactics of small pelagic fishes are important to assess as well as their recruitment success. Such information is particularly relevant in countries where the fishery is critical at socio‐economic level, to better implement fisheries management addressing multiple stressors. 相似文献
67.
Early life history connectivity of Antarctic silverfish (Pleuragramma antarctica) in the Ross Sea 下载免费PDF全文
Cassandra M. Brooks Jilda Alicia Caccavo Julian Ashford Robert Dunbar Kimberly Goetz Mario La Mesa Lorenzo Zane 《Fisheries Oceanography》2018,27(3):274-287
A recent population hypothesis for Antarctic silverfish (Pleuragramma antarctica), a critical forage species, argued that interactions between life history and circulation associated with glacial trough systems drive circumpolar distributions over the continental shelf. In the Ross Sea, aggregations of eggs and larvae occur under fast ice in Terra Nova Bay, and the hypothesis predicted that dispersing larvae encounter outflow along the western side of Drygalski Trough. The outflow advects larvae towards the shelf‐break, and mixing with trough inflow facilitates return toward the inner shelf. To examine the hypothesis, we compared samples of P. antarctica collected near Coulman Island in the outflow, along Crary Bank in the inflow, and a third set taken over the rest of the Ross Sea. We ruled out misidentification using an innovative genetic validation. Silverfish larvae comprised 99.5% of the catch, and the highest population densities were found in Drygalski Trough. The results provided no evidence to reject the population hypothesis. Abundance indices, back‐calculated hatching dates, length distributions and growth were congruent with a unified early life history in the western Ross Sea, constrained by cryopelagic early stages in Terra Nova Bay. By contrast, a sample in the Bay of Whales revealed much smaller larvae, suggesting either a geographically separate population in the eastern Ross Sea, or westward connectivity with larvae spawned nearby by fish sourced from troughs upstream in the Amundsen Sea. These results illustrate how hypotheses that integrate population structure with life history can provide precise spatial predictions for subsequent testing. 相似文献
68.
Fertilizer recommendations for citrus production on heavier textured, calcareous soils increase with the age of the tree up to 168 kg N ha−1 year−1 from a soluble source in one or two applications. Such practices may be inefficient and could cause detrimental effects on fruit quality. A variety of fertilization practices are used in South Texas, and additional evaluation is needed. Rates and sources of nitrogen (N), phosphorus (P), and a commercial program consisting of both a soil component containing organic acids and a foliar component were evaluated on grapefruit production for 6 years. Increasing rate of N application increased leaf nutrient concentration, grapefruit yield, and affected fruit shape as indicated by a decrease in the equatorial:polar diameter (E:P) ratio. Since the response to increasing N application by the E:P ratio was linear while grapefruit yield was quadratic, high N application has a negative effect on fruit shape beyond the level of increasing total yield. Phosphorus application had no effect on any parameter measured, even though soil testing indicated soil P levels were below those at which a P response would be expected. Comparisons between granular 21-0-0 applied broadcast and liquid N-32 injected in a band in the soil at the drip line showed an increase in the number of fruit per tree, but no differences in yield. The ‘Actagro’ program greatly improved available soil P levels and increased the juice brix:acidity ratio, but did not affect grapefruit yield. Phosphorus availability may therefore be a factor in improving grapefruit juice quality. The soil profile within the citrus root zone was found to contain large amounts of inorganic N, but availability of this residual N was limited since yield responses occurred at the N application rates applied in this study. The amount of N contained in the fruit harvested was a small fraction, roughly 5–10%, of the residual amount in the soil. While N uptake in the fruit increased with N fertilizer application, the increase in N taken up relative to the amount applied averaged 42%. 相似文献
69.
70.
Grigera SA Perry RS Schofield AJ Chiao M Julian SR Lonzarich GG Ikeda SI Maeno Y Millis AJ Mackenzie AP 《Science (New York, N.Y.)》2001,294(5541):329-332
The concept of quantum criticality is proving to be central to attempts to understand the physics of strongly correlated electrons. Here, we argue that observations on the itinerant metamagnet Sr3Ru2O7 represent good evidence for a new class of quantum critical point, arising when the critical end point terminating a line of first-order transitions is depressed toward zero temperature. This is of interest both in its own right and because of the convenience of having a quantum critical point for which the tuning parameter is the magnetic field. The relationship between the resultant critical fluctuations and novel behavior very near the critical field is discussed. 相似文献