马尾松人工林施肥试验初报

本文通过对马尾松人工林施肥试验，表明在Ⅲ类地，适量施尿素和过磷酸钙可促进幼林树高、胸径生长。     

A Novel Alginate Lyase: Identification,Characterization, and Potential Application in Alginate Trisaccharide Preparation

Alginate oligosaccharides (AOS) have many biological activities and significant applications in prebiotics, nutritional supplements, and plant growth development. Alginate lyases have unique advantages in the preparation of AOS. However, only a limited number of alginate lyases have been so far reported to have potentials in the preparation of AOS with specific degrees of polymerization. Here, an alginate-degrading strain Pseudoalteromonas arctica M9 was isolated from Sargassum, and five alginate lyases were predicted in its genome. These putative alginate lyases were expressed and their degradation products towards sodium alginate were analyzed. Among them, AlyM2 mainly generated trisaccharides, which accounted for 79.9% in the products. AlyM2 is a PL6 lyase with low sequence identity (≤28.3%) to the characterized alginate lyases and may adopt a distinct catalytic mechanism from the other PL6 alginate lyases based on sequence alignment. AlyM2 is a bifunctional endotype lyase, exhibiting the highest activity at 30 °C, pH 8.0, and 0.5 M NaCl. AlyM2 predominantly produces trisaccharides from homopolymeric M block (PM), homopolymeric G block (PG), or sodium alginate, with a trisaccharide production of 588.4 mg/g from sodium alginate, indicating its promising potential in preparing trisaccharides from these polysaccharides.     

早酥梨病程相关基因非表达子1基因（NPR1）克隆及原核表达

本研究以成年早酥梨(Pyrus bretschneideri cv.Zaosu)叶片为材料,通过RT-PCR克隆早酥梨病程相关基因非表达子1基因(NPR1)并获得其全长序列1771 bp,开放阅读框为1761 bp,编码586个氨基酸(GenBank登录号:FJ769372).利用NCBI/Blastp和ClustalX软件进行相似性分析表明,目的基因编码蛋白质序列与日本梨(p.pyrifolia)、秋子梨(P.ussuriensis)、苹果(Malus xdomestica)、烟草(Nicotiana tabacum)和拟南芥(Arabidopsis thaliana)的NPR1蛋白相似性分别为99%、98%、98%、67%和59%.将其连接pGEX-4T-1原核表达载体并转化大肠杆菌(EScherichia coli)BL21,经IPTG诱导表达获得大小约为91 kD的目的融合蛋白,融合蛋白主要以包涵体形式表达,表达量约占总蛋白17%.     

日光温室光周期控制器的设计

本文设计了一种用于日光温室的光周期控制器 ,解决了日光温室自动化光照的问题。该设计采用数字电路 ,光电传感器 ,电光源 ,实现了对白天光照接续的时间和晚间增补光照的时间的自动、准确的控制     

真姬菇不同菌株的筛选试验

为选择适宜牡丹江地区栽培的高产优质真姬菇菌株,于2006年以木屑为培养料进行了不同真姬菇菌株的比较试验。结果表明,真4号表现最佳,其菌丝生长速度快,长势好,子实体形态好,产量高。经方差分析,生物转化率为82．0％,差异达到极显著水平,适合在本地区推广种植。     

黄薇花粉活力及柱头可授性

  目的   研究不同条件下,黄薇Heimia myrtifolia的花粉萌发及花粉管生长情况。  方法   以黄薇为材料,采用花粉离体萌发法研究不同温度、蔗糖质量浓度和硼酸质量浓度对黄薇花粉萌发及花粉管生长的影响;用联苯胺-过氧化氢法测定柱头可授性,研究不同质量浓度萘乙酸、赤霉素、6-苄基腺嘌呤对黄薇柱头可授性的影响,并对黄薇花粉与柱头进行电镜扫描观察。  结果   ① 不同温度、蔗糖质量浓度、硼酸质量浓度对黄薇花粉的萌发生长影响差异明显,培养黄薇花粉适宜温度为35℃,蔗糖适宜质量浓度为50.0~150.0 g·L-1,硼酸适宜质量浓度为0.5~1.0 g·L-1;②黄薇柱头可授性受时间影响,在开花6~8 h柱头可授性最强;③不同植物生长调节剂对黄薇柱头可授性影响差异明显,提高黄薇柱头可授性最适质量浓度为萘乙酸10.0 mg·L-1、赤霉素200.0~400.0 mg·L-1、6-苄基腺嘌呤20.0 mg·L-1;④黄薇柱头直径为425.78~546.93 μm,花粉极轴长为23.15~26.74 μm,赤道轴长为18.02~20.03 μm,属小型花粉。  结论   黄薇人工授粉的最佳时期在开花后6~8 h,结合一定质量浓度的植物生长调节剂,可以有效提高结实率。     

投喂频率对黄鳝幼鱼摄食、生长及饵料利用效率的影响

研究了不同投喂频率对黄鳝(Monopterus albus)摄食率、特定生长率、饵料效率、体重分化、鱼体组成的影响。试验共设5组不同投喂频率(2、3、4、12次/d和24次/d),每组3个重复,试验持续30 d。结果显示:投喂频率4次/d组的摄食率显著高于其它各组(P<0.05);特定生长率有不同程度增高,4次/d组达到最高(P<0.05);饲料利用效率4次/d组显著高于2次/d组(P<0.05),与其它组无显著性差异(P>0.05);随着投喂频率增加,各试验组组内个体体重无显著差异(P>0.05),黄鳝的规格趋于整齐。试验各组黄鳝的鱼体成分无显著性差异(P>0.05);饵料蛋白质和能量的表观消化率,在4次/d时达到最高(P<0.05)。试验表明:黄鳝的生长受投喂频率的影响,每天投喂4次是黄鳝养殖的最佳投喂频率。     

Cloning and expression of nucleocapsid protein gene of TGEV HB06 strain

The nucleocapsid protein gene of transmissible gastroenteritis virus, 1 149 bp in length, was amplified by RT-PCR from isolated strain HB06 and cloned into pMD18-T. Sequence comparison with other transmissible gastroenteritis virus (TGEV) strains selected from the Gene Bank revealed that the homology of N gene complete sequence shares more than 97% in nucleotide. N gene was cloned into BamHI and EcoRI multiple cloning sites of the prokaryotic expression vector pET 20 b, and named pETN. After being induced by isopropyl-β-D-thiogalactopyranoside (IPTG), the recombinant nucleocapsid protein was expressed. The result of SDS-PAGE and Western-blot showed that the recombinant nucleocapsid protein was 47 kDa and had strong positive reactions with TGEV-specific antibody.     

猪链球菌35个血清型标准抗血清的制备及应用

用猪链球菌35个血清型的标准菌株免疫新西兰白兔制备标准抗血清,经试管凝集测定抗血清的效价均在1:32以上,吸附处理后抗血清具有良好的特异性和敏感性。对12株猪链球菌的试验结果表明:抗血清只与相同血清型的菌株出现凝集,可以区分出PCR方法无法区分的1型和14型以及2型和1/2型,并在国内首次鉴定出猪链球菌13型。