A new multistage dynamic model for biological control exemplified by the host–parasitoid system <Emphasis Type="Italic">Spodoptera exigua</Emphasis>–<Emphasis Type="Italic">Chelonus oculator</Emphasis>

Over the last few decades, important advances have been made in understanding of host–parasitoid relations and their applications to biological pest control. Not only has the number of agent species increased, but new manipulation techniques for natural enemies have also been empirically introduced, particularly in greenhouse crops. This makes biocontrol more complex, requiring a new mathematical modeling approach appropriate for the optimization of the release of agents. The present paper aimed at filling this gap by the development of a temperature- and stage-dependent dynamic mathematical model of the host–parasitoid system with an improved functional response. The model is appropriate not only for simulation analysis of the efficiency of biocontrol agents, but also for the application of optimal control methodology for the optimal timing of agent releases, and for the consideration of economic implications. Based on both laboratory and greenhouse trials, the model was validated and fitted to the data of Chelonus oculator (F.) (Hym.: Braconidae) as a biological control agent against the beet armyworm, Spodoptera exigua Hübner (Lep.: Noctuidae). We emphasize that this model can be easily adapted to other interacting species involved in biological or integrated pest control with either parasitoid or predator agents.     

Predicting stand damage and tree survival in burned forests in Catalonia (North-East Spain)

The study developed models for predicting the post-fire tree survival in Catalonia. The models are appropriate for forest planning purposes. Two types of models were developed: a stand-level model to predict the degree of damage caused by a forest fire, and tree-level models to predict the probability of a tree to survive a forest fire. The models were based on forest inventory and fire data. The inventory data on forest stands were obtained from the second (1989–1990) and third (2000–2001) Spanish national forest inventories, and the fire data consisted of the perimeters of forest fires larger than 20 ha that occurred in Catalonia between the 2nd and 3rd measurement of the inventory plots. The models were based on easily measurable forest characteristics, and they permit the forest manager to predict the effect of stand structure and species composition on the expected damage. According to the stand level fire damage model, the relative damage decreases when the stand basal area or mean tree diameter increases. Conversely, the relative stand damage increases when there is a large variation in tree size, when the stand is located on a steep slope, and when it is dominated by pine. According to the tree level survival models, trees in stands with a high basal area, a large mean tree size and a small variability in tree diameters have a high survival probability. Large trees in dominant positions have the highest probability of surviving a fire. Another result of the study is the exceptionally good post-fire survival ability of Pinus pinea and Quercus suber.     

Lack of a type-2 glycosyltransferase in the fish pathogen Flavobacterium psychrophilum determines pleiotropic changes and loss of virulence

Flavobacterium psychrophilum is an important fish pathogen, responsible for Cold Water Disease, with a significant economic impact on salmonid farms worldwide. In spite of this, little is known about the bacterial physiology and pathogenesis mechanisms, maybe because it is difficult to manipulate, being considered a fastidious microorganism. Mutants obtained using a Tn4351 transposon were screened in order to identify those with alteration in colony morphology, colony spreading and extracellular proteolytic activity, amongst other phenotypes. A F. psychrophilum mutant lacking gliding motility showed interruption of the FP1638 locus that encodes a putative type-2 glycosyltransferase (from here on referred to as fpgA gene, Flavobacterium psychrophilum glycosyltransferase). Additionally, the mutant also showed a decrease in the extracellular proteolytic activity as a consequence of down regulation in the fpgA mutant background of the fpp2-fpp1 operon promoter, responsible for the major extracellular proteolytic activity of the bacterium. The protein glycosylation profile of the parental strain showed the presence of a 22 kDa glycosylated protein which is lost in the mutant. Complementation with the fpgA gene led to the recovery of the wild-type phenotype. LD₅₀ experiments in the rainbow trout infection model show that the mutant was highly attenuated. The pleiotropic phenotype of the mutant demonstrated the importance of this glycosyltranferase in the physiology and virulence of the bacterium. Moreover, the fpgA mutant strain could be considered a good candidate for the design of an attenuated vaccine.     

Population pharmacokinetics of methadone hydrochloride after a single intramuscular administration in adult Japanese sika deer (Cervus nippon nippon)

ObjectiveTo assess the population pharmacokinetics of methadone in deer.Study designProspective non-randomized experimental trial.AnimalsTwelve healthy adult sika deer (nine males and three females).MethodsDeer received intramuscular administration of racemic methadone hydrochloride at 0.5 mg kg⁻¹ or 1 mg kg⁻¹. Plasma methadone and its metabolite 2-Ethylidene-1,5-Dimethyl-3,3-Diphenyl-Pyrolidine (EDDP) concentrations were determined by validated liquid chromatography coupled to tandem mass spectrometry methods, at times 0, 30 minutes, 1, 2, 3, 4, 5, 6, 8, 12 and 24 hours. Population pharmacokinetics analysis was undertaken using a non-linear mixed effects modelling (NONMEM).ResultsA two-compartment linear disposition model best described observed time-concentration profiles of methadone and EDDP. Population parameter estimates of methadone were elimination clearance (17.3 L hour⁻¹), metabolic clearance (34.6 L hour⁻¹), volume of distribution of compartment 1 (216.0 L) and volume of distribution of compartment 2 (384.0 L). Population parameter estimates of EDDP were elimination clearance (121.0 L hour⁻¹), volume of distribution of compartment 3 (1.08 L) and volume of distribution of compartment 4 (499.5 L). The total clearance and total volume of distribution of methadone and EDDP were 51.9 L hour⁻¹, 121.0 L hour ⁻¹, 600.0 L and 500.6 L, respectively. The methadone terminal elimination half-life was 8.19 hours. No adverse effects were observed after methadone administration.Conclusions and Clinical relevanceFollowing intramuscular injection, methadone was characterized by a large total volume of distribution, high systemic clearance and intermediate terminal half-life in sika deer.     

Quantitation of selected odor-active constituents in dry fermented sausages prepared with different curing salts

The odor-active compounds of dry-fermented sausages with added nitrite or nitrate as curing agents were identified by gas chromatography-olfactometry (GC-O) applying the detection frequency (DF) method. The quantification of these compounds in the sausage was determined by multiple headspace solid-phase microextraction (multiple HS-SPME). There were no specific odor-active compounds related to the use of nitrite or nitrate although there were differences in the DF value of several compounds. The nitrite-added sausages presented higher DF values for ethanol, 1-hexanol, propanoic acid, 2-heptenal, and nonanal while the nitrate-added sausages had higher DF values for phenylacetaldehyde and 3-methyl-butanal. Eighteen compounds were quantified by multiple HS-SPME. Most of them were above their air detection thresholds, but only hexanal, heptanal, and 1-octen-3-ol were in a concentration higher than their oil threshold values. These compounds would probably be the main contributors to the aroma of fermented sausages.