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991.
Wager TD Rilling JK Smith EE Sokolik A Casey KL Davidson RJ Kosslyn SM Rose RM Cohen JD 《Science (New York, N.Y.)》2004,303(5661):1162-1167
The experience of pain arises from both physiological and psychological factors, including one's beliefs and expectations. Thus, placebo treatments that have no intrinsic pharmacological effects may produce analgesia by altering expectations. However, controversy exists regarding whether placebos alter sensory pain transmission, pain affect, or simply produce compliance with the suggestions of investigators. In two functional magnetic resonance imaging (fMRI) experiments, we found that placebo analgesia was related to decreased brain activity in pain-sensitive brain regions, including the thalamus, insula, and anterior cingulate cortex, and was associated with increased activity during anticipation of pain in the prefrontal cortex, providing evidence that placebos alter the experience of pain. 相似文献
992.
Much of the controversy concerning the theory of punctuated equilibrium stems from skepticism about the biologic validity of fossil morphospecies, particularly for supposedly simple invertebrate taxa like cheilostome Bryozoa that form the bulk of the fossil record. However, evidence from breeding experiments and protein electrophoresis shows that morphospecific identity of cheilostomes is heritable and that morphospecies are genetically distinct with no indication of morphologically cryptic species. Thus paleontologists can study cheilostome evolution at the species level, and previously demonstrated pattems suggesting punctuated speciation in cheilostomes should be taken at face value. 相似文献
993.
Crawford JM Thomas PM Scheerer JR Vagstad AL Kelleher NL Townsend CA 《Science (New York, N.Y.)》2008,320(5873):243-246
PksA, which initiates biosynthesis of the environmental carcinogen aflatoxin B1, is one of the multidomain iterative polyketide synthases (IPKSs), a large, poorly understood family of biosynthetic enzymes. We found that dissection of PksA and its reconstitution from selected sets of domains allows the accumulation and characterization of advanced octaketide intermediates bound to the enzyme, permitting the reactions controlled by individual catalytic domains to be identified. A product template (PT) domain unites with the ketosynthase and thioesterase in this IPKS system to assemble precisely seven malonyl-derived building blocks to a hexanoyl starter unit and mediate a specific cyclization cascade. Because the PT domain is common among nonreducing IPKSs, these mechanistic features should prove to be general for IPKS-catalyzed production of aromatic polyketides. 相似文献
994.
Control of stochasticity in eukaryotic gene expression 总被引:1,自引:0,他引:1
995.
Shapiro B Drummond AJ Rambaut A Wilson MC Matheus PE Sher AV Pybus OG Gilbert MT Barnes I Binladen J Willerslev E Hansen AJ Baryshnikov GF Burns JA Davydov S Driver JC Froese DG Harington CR Keddie G Kosintsev P Kunz ML Martin LD Stephenson RO Storer J Tedford R Zimov S Cooper A 《Science (New York, N.Y.)》2004,306(5701):1561-1565
The widespread extinctions of large mammals at the end of the Pleistocene epoch have often been attributed to the depredations of humans; here we present genetic evidence that questions this assumption. We used ancient DNA and Bayesian techniques to reconstruct a detailed genetic history of bison throughout the late Pleistocene and Holocene epochs. Our analyses depict a large diverse population living throughout Beringia until around 37,000 years before the present, when the population's genetic diversity began to decline dramatically. The timing of this decline correlates with environmental changes associated with the onset of the last glacial cycle, whereas archaeological evidence does not support the presence of large populations of humans in Eastern Beringia until more than 15,000 years later. 相似文献
996.
The viscosity of colloidal suspensions varies with shear rate, an important effect encountered in many natural and industrial processes. Although this non-Newtonian behavior is believed to arise from the arrangement of suspended particles and their mutual interactions, microscopic particle dynamics are difficult to measure. By combining fast confocal microscopy with simultaneous force measurements, we systematically investigate a suspension's structure as it transitions through regimes of different flow signatures. Our measurements of the microscopic single-particle dynamics show that shear thinning results from the decreased relative contribution of entropic forces and that shear thickening arises from particle clustering induced by hydrodynamic lubrication forces. This combination of techniques illustrates an approach that complements current methods for determining the microscopic origins of non-Newtonian flow behavior in complex fluids. 相似文献
997.
Mocatta D Cohen G Schattner J Millo O Rabani E Banin U 《Science (New York, N.Y.)》2011,332(6025):77-81
Doping of semiconductors by impurity atoms enabled their widespread technological application in microelectronics and optoelectronics. However, doping has proven elusive for strongly confined colloidal semiconductor nanocrystals because of the synthetic challenge of how to introduce single impurities, as well as a lack of fundamental understanding of this heavily doped limit under strong quantum confinement. We developed a method to dope semiconductor nanocrystals with metal impurities, enabling control of the band gap and Fermi energy. A combination of optical measurements, scanning tunneling spectroscopy, and theory revealed the emergence of a confined impurity band and band-tailing. Our method yields n- and p-doped semiconductor nanocrystals, which have potential applications in solar cells, thin-film transistors, and optoelectronic devices. 相似文献
998.
Introduction of invasive species has been implicated in the decline of many native species. However, the mechanisms that underlie successful invasion and species replacement are often poorly understood. We tested the hypothesis that disruption of the natural disturbance regime can facilitate invasion of habitats, resulting in the exclusion of native species. In Arizona’s San Rafael Valley, cattle ponds designed to hold water year-round have replaced seasonal marshes, and this altered drying regime has facilitated invasion by disturbance-intolerant fish and bullfrogs that negatively affect native Sonoran tiger salamanders. We investigated the relationships among pond drying regime, presence of introduced fish and bullfrogs, and presence of tiger salamander populations in 42 ponds. Both fish and breeding bullfrog populations disappeared following pond drying. Pond drying in cattle ponds negatively affected salamanders, but not to the same extent that it affected fish and bullfrogs. Metamorphosed bullfrogs ate salamander larvae in laboratory and field experiments, and the risk of local extinction among salamander populations was increased by introduced fish. Once fish eliminated salamanders from an aquatic habitat, salamanders seldom reappeared unless fish were killed by drying. Simple models were developed to predict change in salamander, fish, and bullfrog distributions with changes in cattle pond drying frequency. The models show that decreases in pond drying frequency could negatively affect salamanders by leading to a community dominated by disturbance-intolerant fish and bullfrogs, but increased drying frequency could negatively affect salamander populations by preventing salamander breeding. These results suggest that manipulation or restoration of disturbance regimes may be a powerful tool in managing for native species threatened by biotic invasions. 相似文献
999.
Dennis S. Ojima William J. Parton David S. Schimel Jonathan M. O. Scurlock Timothy G. F. Kittel 《Water, air, and soil pollution》1993,70(1-4):643-657
We present results from analyses of the sensitivity of global grassland ecosystems to modified climate and atmospheric CO2 levels. We assess 31 grassland sites from around the world under two different General Circulation Models (GCM) double CO2 climates. These grasslands are representative of mostly naturally occurring ecosystems, however, in many regions of the world, grasslands have been greatly modified by recent land use changes. In this paper we focus on the ecosystem dynamics of natural grasslands. The climate change results indicate that simulated soil C losses occur in all but one grassland ecoregion, ranging from 0 to 14% of current soil C levels for the surface 20 cm. The Eurasian grasslands lost the greatest amount of soil C (~1200 g C m?2) and the other temperate grasslands losses ranged from 0 to 1000 g C m?2, averaging approximately 350 g C m?2. The tropical grasslands and savannas lost the least amount of soil C per unit area ranging from no change to 300 g C m?2 losses, averaging approximately 70 g C m?2. Plant production varies according to modifications in rainfall under the altered climate and to altered nitrogen mineralization rates. The two GCM's differed in predictions of rainfall with a doubling of CO2, and these differences are reflected in plant production. Soil decomposition rates responded most predictably to changes in temperature. Direct CO2 enhancement effects on decomposition and plant production tended to reduce the net impact of climate alterations alone. 相似文献
1000.
Arthur H. Johnson Andrew J. Friedland Jonathan G. Dushoff 《Water, air, and soil pollution》1986,30(1-2):319-330
A period of declining growth and extensive mortality of red spruce (Picea rubens Sarg.) has been in progress for the past two decades in the montane forests of New York, Vermont and New Hampshire. Those areas are subject to particularly high levels of pollutant input and there is concern that airborne chemicals have destabilized the forest ecosystems. Field reports indicate that major episodes of mortality also occurred prior to the era of widespread industrial pollution, particularly in the 1870's and 1880's. The present and past periods of mortality have similarities as well as differences, and whether the primary causes are the same cannot be confidently determined with the information currently available. One similarity is that recent and past episodes of red spruce mortality tended to occur during or immediately after periods which are climatically unfavorable for this species. 相似文献