Statistical methods for detecting and comparing periodic data and their application to the nycthemeral rhythm of bodily harm: A population based study

Background  

Animals, including humans, exhibit a variety of biological rhythms. This article describes a method for the detection and simultaneous comparison of multiple nycthemeral rhythms.     

Forest monitoring with TerraSAR-X: first results

Several TerraSAR-X satellite images acquired in high resolution spotlight mode with different polarisations for two study sites in southern Germany were used to distinguish forest from other land cover classes (agriculture, built-up, water bodies) using logistic regression models. In general, we observed that the mean and particularly the standard deviation of the backscatter were viable measures to discriminate land cover classes. Both measures were lowest for water bodies and highest for built-up areas, with agricultural areas and forest in intermediate positions. Trees outside forest were not differentiable from forest with the applied method. The HH-polarised image was better suited for a classification of built-up areas, whereas the VV-polarised image was more appropriate when classifying agricultural areas. Consequently, the combination of the two differently polarised images leads to a significantly better model. Since forests in one study area were generally found on steeper slopes in comparison to other land cover classes, the inclusion of terrain slope further improved the classification, which resulted in an overall accuracy of 92–95%. Systematic differences in the parameter values of the explanatory variables for one class between the study areas may be caused by differing class probabilities. Thus, transferring the model of one study area to the image of another resulted in a 7–9% loss of accuracy.     

Evaluation of anti-Ostertagia ostertagi antibodies in individual milk samples as decision parameter for selective anthelmintic treatment in dairy cows

The aim of this study was to explore whether anti-Ostertagia ostertagi antibody levels measured by ELISA in individual milk samples hold promise as a decision parameter for either herd-level decisions or selective anthelmintic treatments by investigating (1) the relationship between individual and bulk-tank milk ELISA results; (2) the relationships of individual milk ELISA results with non-parasitic cow factors and (3) the relationship between individual milk ELISA results and the milk production response after anthelmintic treatment. Twelve farms were randomly allocated to a whole-herd treatment with eprinomectin or a placebo in October 2004 and individual milk samples and a bulk-tank milk sample were collected 1 month before and 1 month after treatment. Linear mixed models were used to investigate the associations of ELISA results with (a) breed, actual milk production, lactation stage, somatic cell count, age and sampling month and (b) the milk production response after anthelmintic treatment. There was a reasonable correlation between the mean individual and bulk-tank milk ELISA results (r = 0.72). Individual cow ELISA results increased with higher lactation number and were lower in November than September. The associations with the other non-parasitic factors were weak and not significant. Milk yield responses to anthelmintic treatment were greater when treatment was given in early lactation and increased with the pre-treatment ELISA result and cow age. However, these latter two interaction terms were not significant when they were put in the model together. We conclude that (1) O. ostertagi ELISA results from individual milk samples may provide more information on the herd's parasitic status than a single bulk-tank milk result; (2) lactation number should be taken into account when interpreting ELISA results from individual milk samples and (3) the value of the O. ostertagi antibody level in individual cow milk samples to predict individual production responses after anthelmintic treatment remains equivocal.     

Ultrasonographic characterization of the urinary bladder in sows with and without urinary tract infection

The urinary bladders of sows (n=10) without urinary tract infection (UTI) were longitudinally transrectally scanned after emptying and refilling with 200, 400, 600 and 800mL saline, and a volume dependence was found for bladder depth (BD), dorsal (dWT) and ventral wall thicknesses (vWT), wall regularity (WR) and mucosal wall surface (mWS). When another 31 sows without and 15 with UTI (as defined on the basis of high bacterial count and macroscopic/biochemical urine abnormalities) were compared for these parameters using BD as volume equivalent, no differences were found. Sows with UTI more often had moderate to high amounts of sediment than animals without UTI. Ultrasonographic assessment of dWT, vWT, WR and mWS of the urinary bladder of sows requires knowledge of bladder volume, and BD may be used as a volume equivalent. However, the parameters are inappropriate for the diagnosis of UTI as defined in this study, while moderate/high amounts of sediment seem to be indicative. Sediment can be visualized by transrectal scanning, but this is also possible using the transcutaneous route.     

Is there need for leaf-galling grape phylloxera control? Presence and distribution of Dactulosphaira vitifoliae in Swiss vineyards

Grape phylloxera (Dactulosphaira vitifoliae, FITCH) was a major pest in Vitis vinifera grape production in the late 19th and early 20th century in Europe. Roots of V. vinifera varieties are susceptible to D. vitifoliae attack whereas the leaves are not affected. The pest has been controlled by using American grapevine varieties as rootstocks. However, in recent years, grape producers have reported higher incidences of leaf galls induced by D. vitifoliae on V. vinifera leaves. Research on the occurrence of D. vitifoliae on grapevine leaves and roots has shown that D. vitifoliae is present in all wine-growing regions of Switzerland. In more than 80% of vineyards claiming to be infested, D. vitifoliae was found on leaves of varieties with interspecific crosses in their parentage. Three locations with leaf-galling individuals on V. vinifera were detected. D. vitifoliae individuals were found emerging from soil potentially associated with the commonly used rootstock varieties, which are assumed to be phylloxera tolerant. As climate change may necessitate the relocation of some viticultural areas to new wine-growing regions, there will be a greater need to control D. vitifoliae on leaves of interspecific crosses and the inspected planting material to limit further spread of the pest.     

Evaluation of ecological doses of the nitrification inhibitors 3,4-dimethylpyrazole phosphate (DMPP) and 4-chloromethylpyrazole (ClMP) in comparison to dicyandiamide (DCD) in their effects on dehydrogenase and dimethyl sulfoxide reductase activity in soils

Risk assessment of the nitrification inhibitors (NIs) 3,4-dimethylpyrazole phosphate (DMPP), 4-chloromethylpyrazole (ClMP), and dicyandiamide (DCD) on nontarget microbial activity in soils was determined by measuring dehydrogenase and dimethyl sulfoxide reductase activity (DHA, DRA, respectively) in three differently textured soils under laboratory conditions. Dehydrogenase activity was measured with standard procedure recommended to evaluate side effects of environmental chemicals on general microbial activity in soils. The kinetic of inhibition were obtained by dose–response relationships and used to calculate the no observable effect levels (NOEL values) and the effective doses at 10% and 50% inhibition (ED₁₀ and ED₅₀), respectively. Negative effects on DHA and DRA, respectively, were observed only at rates approximately 40–100 times higher than the concentrations recommended in the field. Both DHA and DRA were affected more in the sandy than in the silty or clayey soil. Consequently, NOEL, ED₁₀, and ED₅₀ values were considerably higher in the clayey than in the silty or sandy soil. The heterocyclic N compounds DMPP and ClMP, respectively, were more effective in inhibiting DHA and DRA than DCD. At application rates used in the field as well as at concentration up to 25 to 90 times higher, the NIs concerned failed to affect general soil microbial activity in soils. Among the three NIs tested, the not marketed ClMP exhibited the strongest negative effects on soil microbial activity. At recommended application rates, the NIs tested should be considered as enviromentally safe.