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231.
Eric Juskewitz Ekaterina Mishchenko Vishesh K. Dubey Marte Jenssen Martin Jakubec Philip Rainsford Johan Isaksson Jeanette H. Andersen Johanna U. Ericson 《Marine drugs》2022,20(5)
Treatment options for infections caused by antimicrobial-resistant bacteria are rendered ineffective, and drug alternatives are needed—either from new chemical classes or drugs with new modes of action. Historically, natural products have been important contributors to drug discovery. In a recent study, the dimeric naphthopyrone lulworthinone produced by an obligate marine fungus in the family Lulworthiaceae was discovered. The observed potent antibacterial activity against Gram-positive bacteria, including several clinical methicillin-resistant Staphylococcus aureus (MRSA) isolates, prompted this follow-up mode of action investigation. This paper aimed to characterize the antibacterial mode of action (MOA) of lulworthinone by combining in vitro assays, NMR experiments and microscopy. The results point to a MOA targeting the bacterial membrane, leading to improper cell division. Treatment with lulworthinone induced an upregulation of genes responding to cell envelope stress in Bacillus subtilis. Analysis of the membrane integrity and membrane potential indicated that lulworthinone targets the bacterial membrane without destroying it. This was supported by NMR experiments using artificial lipid bilayers. Fluorescence microscopy revealed that lulworthinone affects cell morphology and impedes the localization of the cell division protein FtsZ. Surface plasmon resonance and dynamic light scattering assays showed that this activity is linked with the compound‘s ability to form colloidal aggregates. Antibacterial agents acting at cell membranes are of special interest, as the development of bacterial resistance to such compounds is deemed more difficult to occur. 相似文献
232.
Søren Kirkegaard Nielsen Hans Christian Carstensen Lars J. Munkholm Michael Nørremark Jesper Rasmussen Johanna Bertl Rodrigo Labouriau Ole Green 《Soil Use and Management》2020,36(3):470-481
In inversion tillage systems, the mouldboard plough is fundamental for producing a desirable seedbed. The desired ploughing quality is achieved when the plough layer is inverted homogeneously. This is, however, difficult to obtain in the main-headland intersection zone where the plough is lowered and elevated, as ploughed and unploughed triangles are formed. This results in zones where the soil is inverted twice, which may result in poor residue and weed incorporation and a poor seedbed quality. The design of the three-point linkage-attached mouldboard plough has not changed since the 1950s, but the number of furrows has increased, which has increased the size of the aforementioned triangles. A novel ploughing system was introduced to meet these headland challenges, where each plough section can be lowered and elevated independently. The aim of this study was to evaluate the effects of using a section-controlled mouldboard plough. Two similarly designed, randomized, field plot experiments were conducted on two different soil types (sandy loam and loamy sand) on a stubble field and grass field. The study showed that the section-controlled plough reduced the main-headland overlap area by ~98%. The results of a range of soil physical properties measurements and seedbed quality analyses showed that the section-controlled plough created a homogeneous loosened seedbed quality, improving the incorporation of crop residues and leaving fewer residues on the soil surface. Furthermore, the section-controlled plough showed additional benefits, for example wedge operations and visual line marking. 相似文献
233.
Proschogo NW Albertson PL Bursle J McConchie CA Turner AG Willett GD 《Journal of agricultural and food chemistry》2012,60(8):1973-1980
High-resolution electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry is successfully used in the detailed molecular analysis of aged macadamia nut oils. The results are consistent with peroxide values, the current industry measure for rancidity, and provide detailed molecular information on the oxidative and hydrolytic degeneration of such oils. Mass analysis of macadamia oil samples stored for extended periods at 6 °C revealed that oils obtained by the cold press method are more susceptible to aging than those obtained using modified Soxhlet or accelerated solvent extraction methods. 相似文献
234.
235.
Tuber shape is a genetically determined, important varietal characteristic that is also influenced by environmental factors.
In this study, the overall dimensions of tubers of two potato cultivars (Van Gogh and Yukon Gold) were determined using a
triaxial ellipsoid model that was found to encompass the main varietal differences. The more detailed surface features were
captured using a spherical-harmonics series representation for the residual undulation. The method was then applied to four
additional cultivars (Bellona, Lady Rosetta, Pito, and Sabina). Modelling the undulation of tuber surface in addition to the
overall dimensions indicated that cultivars differed in the global shape and, in specific cases, also in the surface undulation
of tubers. The new, improved method for modelling tuber shapes can thus capture and distinguish the overall three-dimensional
shape and irregularities caused by growth conditions and other factors. It is envisaged to be useful for quantitative genetics
approaches aiming to resolve the relative impact of different genes on tuber shape, as well as for various machine vision
and other applications. 相似文献
236.
Important pest species of the Spodoptera complex: Biology,thermal requirements and ecological zoning
Parra Jos Roberto Postali Coelho Aloisio Cuervo-Rugno Johanna Bajonero Garcia Adriano Gomes de Andrade Moral Rafael Specht Alexandre Neto Durval Dourado 《Journal of pest science》2022,95(1):169-186
Journal of Pest Science - In South America, especially in Brazil, four members of the Spodoptera complex, Spodoptera albula (Walker, 1857), S. cosmioides (Walker, 1858), S. eridania (Stoll, 1782),... 相似文献
237.
Monogioudi E Permi P Filpponen I Lienemann M Li B Argyropoulos D Buchert J Mattinen ML 《Journal of agricultural and food chemistry》2011,59(4):1352-1362
Cross-linking of β-casein by Trichoderma reesei tyrosinase (TrTyr) and Streptoverticillium mobaraense transglutaminase (Tgase) was analyzed by (31)P nuclear magnetic resonance (NMR) spectroscopy in ionic liquid (IL). According to (31)P NMR, 91% of the tyrosine side chains were cross-linked by TrTyr at high dosages. When Tgase was used, no changes were observed because a different cross-linking mechanism was operational. However, this verified the success of the phosphitylation of phenolics within the protein matrix in the IL. Atomic force microscopy (AFM) in solid state showed that disk-shaped nanoparticles were formed in the reactions with average diameters of 80 and 20 nm for TrTyr and Tgase, respectively. These data further advance the current understanding of the action of tyrosinases on proteins on molecular and chemical bond levels. Quantitative (31)P NMR in IL was shown to be a simple and efficient method for the study of protein modification. 相似文献
238.
239.
Sambuu R Takagi M Shiga S Uno S Kokushi E Namula Z Otoi T Miyamoto A Deguchi E Fink-Gremmels J 《The Journal of reproduction and development》2011,57(2):303-306
Zearalenone (ZEN) and its metabolites are important nonsteroidal estrogenic mycotoxins that cause reproductive disorders in domestic animals, especially pigs. We aimed to simultaneously detect ZEN and its metabolites á-zearalenol (α-ZOL) and β-zearalenol (β-ZOL) in porcine follicular fluid (FF) by liquid chromatography-tandem mass spectrometry. ZEN and α-ZOL, but not β-ZOL, were detected in all pooled FF samples collected from coexisting follicles (diameter ≥ 6 mm) within 10 ovaries. Furthermore, ZEN and α-ZOL were detected in samples pretreated with β-glucuronidase/arylsulfatase, but not in those left untreated, suggesting that the FF samples contained glucuronide-conjugated forms of the mycotoxins that may be less harmful to porcine oocytes due to glucuronidation affecting the receptor binding. Nonetheless, the effects of the glucuronide-conjugated forms should be studied, both in vitro and in vivo. 相似文献
240.
Friedrich A Szabo I Dorn C Schroeter A Jaber M Berendonk G Brom M Ledwolorz J Malorny B Helmuth R 《Berliner und Münchener tier?rztliche Wochenschrift》2011,124(9-10):401-410
The present report deals with Salmonella strains received at the German National Reference Laboratory for Salmonella (NRL-Salm) for routine diagnostic in the year 2009. Hence, the working group continues the previous report from Friedrich et al. (2010) about the documentation on the serovar distribution of Salmonella received at the NRL-Salm in the years 2004-2008. As in the recent years, most of the Salmonella strains originated from livestock and food. In the year 2009 the NRL-Salm received 4765 isolates, most of them (85,1 %) were routine diagnostic. Salmonella ser. Typhimurium, its monophasic variant S. enterica subspecies enterica serovar 1,4,[5],12:i:- and Salmonella ser. Enteritidis were the most prevalent serovars. The number of S. enterica subsp. enterica serovar 1,4,[5],12:i:- isolates increased in 2009, in comparison to the years 2004-2008, and became the second most prevalent serovar serotyped at the NRL-Salm. 相似文献