Carbon flow into microbial and fungal biomass as a basis for the belowground food web of agroecosystems

The origin and quantity of plant inputs to soil are primary factors controlling the size and structure of the soil microbial community. The present study aimed to elucidate and quantify the carbon (C) flow from both root and shoot litter residues into soil organic, extractable, microbial and fungal C pools. Using the shift in C stable isotope values associated with replacing C3 by C4 plants we followed root- vs. shoot litter-derived C resources into different soil C pools. We established the following treatments: Corn Maize (CM), Fodder Maize (FM), Wheat + maize Litter (WL) and Wheat (W) as reference. The Corn Maize treatment provided root- as well as shoot litter-derived C (without corn cobs) whereas Fodder Maize (FM) provided only root-derived C (aboveground shoot material was removed). Maize shoot litter was applied on the Wheat + maize Litter (WL) plots to trace the incorporation of C4 litter C into soil microorganisms. Soil samples were taken three times per year (summer, autumn, winter) over two growing seasons. Maize-derived C signal was detectable after three to six months in the following pools: soil organic C (C_org), extractable organic C (EOC), microbial biomass (C_mic) and fungal biomass (ergosterol). In spite of the lower amounts of root- than of shoot litter-derived C inputs, similar amounts were incorporated into each of the C pools in the FM and WL treatments, indicating greater importance of the root- than shoot litter-derived resources for the soil microorganisms as a basis for the belowground food web. In the CM plots twice as much maize-derived C was incorporated into the pools. After two years, maize-derived C in the CM treatment contributed 14.1, 24.7, 46.6 and 76.2% to C_org, EOC, C_mic and ergosterol pools, respectively. Fungi incorporated maize-derived C to a greater extent than did total soil microbial biomass.     

Anatomy and dynamics of a supramolecular membrane protein cluster

Most plasmalemmal proteins organize in submicrometer-sized clusters whose architecture and dynamics are still enigmatic. With syntaxin 1 as an example, we applied a combination of far-field optical nanoscopy, biochemistry, fluorescence recovery after photobleaching (FRAP) analysis, and simulations to show that clustering can be explained by self-organization based on simple physical principles. On average, the syntaxin clusters exhibit a diameter of 50 to 60 nanometers and contain 75 densely crowded syntaxins that dynamically exchange with freely diffusing molecules. Self-association depends on weak homophilic protein-protein interactions. Simulations suggest that clustering immobilizes and conformationally constrains the molecules. Moreover, a balance between self-association and crowding-induced steric repulsions is sufficient to explain both the size and dynamics of syntaxin clusters and likely of many oligomerizing membrane proteins that form supramolecular structures.     

Surface of young Jupiter family comet 81P/Wild 2: view from the Stardust Spacecraft

Images taken by the Stardust mission during its flyby of 81P/Wild 2 show the comet to be a 5-kilometer oblate body covered with remarkable topographic features, including unusual circular features that appear to be impact craters. The presence of high-angle slopes shows that the surface is cohesive and self-supporting. The comet does not appear to be a rubble pile, and its rounded shape is not directly consistent with the comet being a fragment of a larger body. The surface is active and yet it retains ancient terrain. Wild 2 appears to be in the early stages of its degradation phase as a small volatile-rich body in the inner solar system.     

X-ray imaging of shock waves generated by high-pressure fuel sprays

Synchrotron x-radiography and a fast x-ray detector were used to record the time evolution of the transient fuel sprays from a high-pressure injector. A succession of 5.1-microsecond radiographs captured the propagation of the spray-induced shock waves in a gaseous medium and revealed the complex nature of the spray hydrodynamics. The monochromatic x-radiographs also allow quantitative analysis of the shock waves that has been difficult if not impossible with optical imaging. Under injection conditions similar to those found in operating engines, the fuel jets can exceed supersonic speeds and result in gaseous shock waves.     

Increased grain yield and micronutrient concentration in transgenic winter wheat by ectopic expression of a barley sucrose transporter

Optimising assimilate partitioning to spikes is important to increase wheat yield potential. Novel winter wheat lines (HOSUT), ectopically expressing barley sucrose transporter HvSUT1 controlled by the barley Hordein B1 promoter were used to evaluate the potential of improved sucrose uptake capacity on grain yield and quality under field-like conditions. Three independent HOSUT lines were grown over three years in micro-plots. Grain yield per plot was significantly increased by 28%, together with higher protein yield per plot and higher iron and zinc concentration when compared to the non-transformed control wheat Certo. Thousand grain weight was enhanced, indicating that expression of HvSUT1 increases individual grain sink strength. HOSUT grains are larger, display increased grain width and to a lesser extent grain length, indicating transgene effects at a stage when grains grow under filial control. Grain number per spike was decreased by 15% and protein contents by 5%, on average, especially that of glutenins. Overall, despite some compensating effects on grain number per spike and protein contents, HOSUT lines generate a significant yield advantage. The findings can contribute to understanding determinants of grain size and number and its relationship to grain sink strength and might identify limitations of wheat yield potential.     

Wachstum und Ernährung von Eberesche,Buche und Fichte im Gefäßversuch mit immissionsbelasteten Bodensubstraten aus dem Erzgebirge

Influence of simulated sulphur and nitrogen depositions on biomass and nutrient relationships of mountain ash, European beech and Norway spruce in a pot experiment with two soil substrates from the Ore Mountains Growth and nutrient relationships of mountain ash (Sorbus aucuparia), European beech (Fagus sylvatica) and Norway spruce (Picea abies) were investigated in a pot experiment with the two substrates rhyolithe (acidic) and basalt (alkaline). Additionally, depositions of sulphur (S) and nitrogen (N) as expected to occur in the Ore Mountains (Saxonia, Germany) in the future were simulated in order to test the species′ suitability for forest regeneration. After two years, aboveground biomass was significantly higher on basalt compared to rhyolithe for all species. The amount of S given (0 and 100 kg ha^—1 a^—1) and the dominant form of N applied (NH₄⁺:NO₃^— = 1:4 and 4:1; total amount of N given 80 kg ha^—1 a^—1) were of minor influence only. The contents of N and K in leaves or needles were higher on rhyolithe, whereas P, Ca, Mg and Mn contents were higher on basalt. Nutrient contents were only slightly affected by the amount of S supply and the NH₄⁺:NO₃^— ratio. In contrast to mountain ash, beech saplings exhibited considerable growth on rhyolite. It is therefore concluded that beech may be a suitable species for forest regeneration even on acidic soils suffering heavily from S and N immissions, whereas spruce will not tolerate high S loads. However, increasing N depositions by stimulating growth may cause deficiencies of Mg and K in both species.     

Productivity of mixed versus pure stands of oak (Quercus petraea (Matt.) Liebl. and Quercus robur L.) and European beech (Fagus sylvatica L.) along an ecological gradient

The mixture of beech (Fagus sylvatica L.) and oak (sessile oak, Quercus petraea (Matt.) Liebl., and pedunculate oak, Q. robur L.) is of considerable importance in Europe and will probably become even more important under climate change. Therefore, the performance of oak and beech in mixture was compared with the species’ growth in pure stands. Data from 37 long-term mixing experiments in Poland, Germany and Switzerland were pooled for analysis of mixing effects on stand productivity and possible interrelationships with mixing portions or site conditions. We found that on average, mixed stands of oak and beech exceeded biomass productivity in pure stands by 30 % or 1.7 t ha^?1 year^?1, as the growth of both species was benefitted by the mixture. However, that the interaction actually ranged from facilitation and overyielding on poor sites to underyielding on fertile sites triggered by competition. An empirically derived interaction model showed volume and dry mass growth changing in mixed stands from gains of 50 % to losses of 10 % depending on site conditions. It is concluded that the analysed mixture grows in accordance with the stress-gradient hypothesis and that our results suggest a site-specific relationship between species mixture and biomass productivity. As a consequence, an adequate species mix should result in increased productivity under steady state as well as climate change.     

Targeted genome modifications in cereal crops

The recent advent of customizable endonucleases has led to remarkable advances in genetic engineering, as these molecular scissors allow for the targeted introduction of mutations or even precisely predefined genetic modifications into virtually any genomic target site of choice. Thanks to its unprecedented precision, efficiency, and functional versatility, this technology, commonly referred to as genome editing, has become an effective force not only in basic research devoted to the elucidation of gene function, but also for knowledge-based improvement of crop traits. Among the different platforms currently available for site-directed genome modifications, RNA-guided clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) endonucleases have proven to be the most powerful. This review provides an application-oriented overview of the development of customizable endonucleases, current approaches to cereal crop breeding, and future opportunities in this field.