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81.
82.
Jo Toia 《Australian veterinary journal》2002,80(12):725-725
83.
Janaina O Luna Manoel A A Santos Edison L Durigon Jo?o P Araújo Júnior José M B Duarte 《Journal of zoo and wildlife medicine》2003,34(4):414-415
Esophageal-pharyngeal fluids from 53 free-ranging marsh deer (Blastocerus dichotomus) captured for a research program in the state of Mato Grosso do Sul, Brazil, were assayed for tuberculosis. Total DNA was extracted. amplified by polymerase chain reaction using specific primers for Mycobacterium tuberculosis complex (M. tuberculosis, M. bovis, M. microti, and M. africanum), and observed by agarose gel electrophoresis stained with ethidium bromide. All samples were negative. This, along with necropsy and histopathology data, suggests that these animals are not shedding and probably do not have active disease. 相似文献
84.
Vrieling H 《Tijdschrift voor diergeneeskunde》2007,132(1):979-980
85.
Kawasaki PM Kano FS Tamekuni K Garcia JL Marana ER Vidotto O Vidotto MC 《Research in veterinary science》2007,83(3):347-354
Anaplasmosis, caused by Anaplasma marginale, results in significant economic losses of cattle in tropical and subtropical regions worldwide. Six major surface proteins (MSPs) were well characterized and designated as MSP1, MSP2, MSP3, MSP4, and MSP5. The objective of this study was to evaluate the humoral immune response of BALB/c mice against the recombinant MSPs, incorporated into immunostimulating complex (ISCOM). The recombinant proteins purified by Ni-NTA columns were incorporated into ISCOM and ISCOMATRIX by the lipid film hydration method. BALB/c mice immunized with ISCOM/rMSPs and ISCOMATRIX/rMSPs vaccines produced whole IgG, IgG1, and IgG2a, in contrast to the negative groups (PBS and ISCOMATRIX adjuvant). All groups that received antigen responded specifically against the rMSPs by Western blotting, showing the rMSP1a (60-105kDa), rMSP1b (100kDa), rMSP4 (47kDa), and rMSP5 (29kDa). Additional studies will have to be performed in cattle to evaluate the humoral and cellular mechanisms of this subunit vaccine and their possible use as protective vaccines against homologous and heterologous strains of A. marginale. 相似文献
86.
Effect of incubation temperature regimes and culture medium on broccoli microspore culture embryogenesis 总被引:8,自引:0,他引:8
João Carlos da Silva Dias 《Euphytica》2001,119(3):389-394
Conditions for reliable induction of embryogenesis from isolated microspores were studied in ten genotypes of broccoli. Embryo
yields were significantly increased in almost all of the broccoli genotypes by the incubation at 32.5 °C for 1 day, than when the standard incubation at 30 °C for 2 days was used. Treatments of 48 hours at 32.5 °C produced less than optimal results suggesting that broccoli microspores are more sensitive to high temperatures than those
of B. napus. The use of the 1/2 NLN-13 medium yielded greater number of embryos than the standard NLN-13. The magnitude of the response to the redution of
the concentration of major salts by half in the NLN medium varied with the different genotypes. High embryogenic broccoli
cultivars, such as ‘Shogun’, ‘SDB9’, and ‘Green Valiant’, presented a better response to the reduction of the concentration
of major salts by half in NLN-13. Reduction never produced a detrimental effect on embryo yield and seems not to have any
effect in the subsequent development of embryos in plants.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
87.
Summary Phytotron experiments have been carried out to study the effect of different temperatures on the seed set of tetraploid freesias during the raising of the plants and at the time of flowering and pollination.The effect of a relatively low temperature during the growth period is reflected in a comperatively greater number of flowers as a result of an increase in number of ramifications of the peduncle. This sometimes promotes the seed yield notwithstanding that the lower temperature reduces the fertility of each seperate flower. It is clearly proved that the temperature during flowering and pollination affects the fertility of the flower and the ultimate seed yield of the plant. Experiments in our phytotron showed 14°C during the growth period and 20°C at the time of pollination to be the optimum combination for a good seed yield.The effect of a lower night temperature was generally favourable, particularly so when measured by the number of seeds per pollinated flower. 相似文献
88.
RAPD genetic diversity of Albanian olive germplasm and its relationships with other Mediterranean countries 总被引:4,自引:0,他引:4
Angjelina Belaj Zlatko Satovic Hajri Ismaili Dhimitër Panajoti Luis Rallo Isabel Trujillo 《Euphytica》2003,130(3):387-395
RAPD markers were used for the study of 19Albanian olive cultivars and two wild olives (oleasters). A total of 76polymorphic
bands (4.8 polymorphic markers per primer) out of 107 reproducible were obtained using 16 primers. The number of bands per
primer ranged from 4 to 10,whereas the number of polymorphic bands ranged from 1 to 9, corresponding to 71%of the total amplification
products. All the accessions could be identified by the combination of four primers: OPA-19;OPA-02; OPK-16 and OPP-19. The
dendrogram,based on Jaccard's index, included three major groups according to their origin: 1)most of the cultivars from the
area of Berat (South of Albania) 2) cultivars from the Centre and Centre-North of Albania and3) cultivars from the Centre
and North-West of Albania along with the oleaster from Elbasan. In order to evaluate the origin of Albanian cultivars they
were compared to those diffused in other countries like Greece, Italy and Turkey, due to geographical and historical affinity
among these countries, by using a one way AMOVA. Although most of the genetic diversity was attributable to differences among
cultivars within each country (91.47%) significantφ-values among countries(φst = 0.085; p < 0.001)suggested the existence of RAPD phenotypic differentiation. Significant φ-values in all pairs formed by Albania with
the other countries were observed. These results are consistent with the autochthonous origin of Albanian cultivars.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
89.
Summary Genetics of resistance to Ascochyta fabae Speg. in Vicia faba L. was studied with a final objective to develop resistant faba bean varieties to Ascochyta blight. The study was conducted separately on 3 single spore isolates (AF10-2 and AF13-2 from Tunisia and AF4-3 from France) and belonging to different groups of virulence (GV1 and GV2). Important general combining ability (GCA) effects were found especially with isolates AF10-2 and AF4-3. Specific combining ability (SCA), although significant for the 3 isolates, was important only with AF13 -2, but less important than GCA. Additive gene effects were predominant to non-additive effects. Lines 29H and A8817 transmitted to their progenies resistance to the 3 isolates, whereas 14–12 and 19TB conferred resistance to their progenies only with isolates AF13-2 and AF4-3, respectively. In the material studied, resistance was generally controlled by dominant genes but also could be attributed to recessive genes although less frequent. Analysis of segregation in the F2 of 2 crosses between the resistant lines (A8817 and 29H) and the susceptible line (14–12) with isolate AF4-3 revealed dominant monogenic control at the level of leaves in the 2 resistant lines and, in addition, a recessive gene controlling resistance of stems. Non-allelic interactions were occasionally manifested and their origin appeared to be due to line 19TB. A recurrent selection scheme was proposed with the objective to develop improved open-pollination populations and synthetic varieties responding to the objective of the national Tunisian research programme on faba bean. 相似文献
90.
Ricardo Tadeu de Faria Deonisio Destro João Carlos Bespalhok Filho Rolf Dieter Illg 《Euphytica》2002,124(1):59-63
The goal of this research was to study the introgression of the high regeneration capacity of Lycopersicon pimpinellifolium Mill line WV-700, in recalcitrant tomato cultivars (L. esculentum Mill cvs. Petomech, Santa Rita and VFN-8) using backcrossing. Hypocotyl explants of in vitro germinated seeds were cultivated in half strength MS medium supplemented with 5mg/l 6-BA to assess their shoot regeneration
capacity. The apical shoot of the in vitro germinated seeds were cultured on a separate medium. Apical shoots from genotypes showing high regeneration rates were acclimated
in a glasshouse and used as pollen donors for the next backcrossing. After four backcrossings, the material showed a similar
mean fruit weight for the cultivated tomato and a high regeneration capacity similar to the wild species. It is shown that
L. pimpinellifolium can be used with success as donor parent to introgress in vitro regeneration capacity to recalcitrant tomato cultivars.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献