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21.
Jin Joo Hue Hu-Jang Lee Sangyong Jon Sang Yoon Nam Young Won Yun Jong-Soo Kim Beom Jun Lee 《Journal of veterinary science (Suw?n-si, Korea)》2013,14(4):473-479
Free Cy5.5 dye and Cy5.5-labeled thermally cross-linked superparamagnetic iron oxide nanoparticles (TCL-SPION) have been routinely used for in vivo optical imaging. However, there is little information about the distribution and accumulation of free Cy5.5 dye and Cy5.5-labeled TCL-SPION in the tissues of mice. Free Cy5.5 dye (0.1 mg/kg body weight) and Cy5.5-labeled TCL-SPION (15 mg/kg body weight) were intravenously injected into the tail vein of ICR mice. The biodistribution and accumulation of the TCL-SPION and Cy5.5 were observed by ex vivo optical imaging and fluorescence signal generation at various time points over 28 days. Cy5.5 dye fluorescence in various organs was rapidly eliminated from 0.5 to 24 h post-injection. Fluorescence intensity of Cy5.5 dye in the liver, lung, kidney, and stomach was fairly strong at the early time points within 1 day post-injection. Cy5.5-labeled TCL-SPION had the highest fluorescence density in the lung at 0.5 h post-injection and decreased rapidly over time. Fluorescence density in liver and spleen was maintained over 28 days. These results suggest that TCL-SPION can be useful as a carrier of therapeutic reagents to treat diseases by persisting for long periods of time in the body. 相似文献
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23.
Chandrappa Gangaiah Amjad A. Ahmad Hue V. Nguyen 《Communications in Soil Science and Plant Analysis》2016,47(17):2046-2052
Nutritional status of vegetable crops is often monitored by analysis of dried plant tissues, which is costly and often time consuming. Two greenhouse trials were conducted, at the University of Hawaii at Manoa, Magoon facilities, to evaluate the portable cardy ion meter (CIM) in determining potassium (K+) status in fresh petiole sap of pak choi as compared with standard laboratory methods. In the first greenhouse trial, three algae species (Gracilaria salicornia, Kappaphycus alvarezii, and Eucheuma denticulatum) were used to apply five rates of K+ (0, 84, 168,252, and 336 kg.ha?1). The pak choi was directly seeded into 4 L pots and was grown in peat moss. In the second greenhouse trial, K+ was provided through Eucheuma denticulatum and potassium nitrate (KNO3) at five rates (0,112, 168, 224, 280, and 336 kg.ha?1) in peat moss and soil media. At harvest, K+ concentrations in fresh petiole sap were analyzed immediately with CIM and the dried samples were analyzed by inductively coupled plasma spectroscopy (ICP) measurement. The results showed increase in leaf K+ content at higher rates and the maximum concentration of leaf K+ at 4500–5300 mg/L for sap and 8–9% for tissue was obtained when K+ was provided between 224 and 284 kg.ha?1. There was a close correlation between the CIM readings and the ICP method (r = 0.8048 and 0.8314) from the first and second GH results, respectively. The results suggest that the CIM could be used for the rapid monitoring of the relative K+ status of plants. The data further suggested 4500–5000 mg K/L for fresh petiole sap and 7.5% K+ in tissue as critical levels for K+ concentration in pak choi. 相似文献
24.
Thi Hue Cao Thi Tu Oanh Nguyen Thi Minh Hang Nguyen Nguyen Thanh Le R. G. Razumovskaya 《Journal Of Aquatic Food Product Technology》2017,26(10):1293-1302
In this study, gelatin was obtained from the scales of seabass (Lates calcarifer) and grey mullet (Mugil cephalus) collected in Vietnam by using Alcalase® 2.4 L FG for pretreatment and electrochemically activated water with pH 2.0–2.5 for extraction. The characteristics and physicochemical properties of gelatin were investigated. The results indicated that the gelatin had a high protein content (88.6–90.0%) with ash (1.43–1.55%) and no fat. The gelatin was identified as type A due to its pH value. The yields of gelatin were 14.1–15.2%. The gel strength and melting point were 270.3 g and 26.7°C, respectively, for gelatin from seabass scales and 249.1 g and 25.9°C, respectively, for samples from grey mullet scales. In contrast, the viscosities of gelatin were found to be 6.97 cP for seabass and 8.73 cP for grey mullet. Both gelatins contained α-chain and β-chain as the major components. Circular dichroism spectra indicated that the triple helical structure in collagen was almost transformed to the random coil structure in gelatin. The proportion of imino acids was high (197–205 residues/1,000 residues). So, gelatin from seabass and grey mullet scales could be used as a potential replacement for mammalian gelatin. 相似文献
25.
Subsoil acidity is a serious constraint to crop production, and is difficult to correct by conventional liming practices. Thus, a different approach to ameliorating acid subsoils was evaluated. Subsoil material of an acid Ultisol (pH 4.4) was packed into 50-cm long columns, then leached with solutions of CaCl2, CaCO3 (suspension) or Ca fulvates prepared from chicken manure, cowpea green manure, or sewage sludge. The total water applied was 30.26 cm (or 800 ml) in 2 days. Thereafter, the columns were dismantled and cut into 5-cm segments for chemical analysis. The results indicated that only 2% of the added Ca from CaCO3 moved past the 15-cm depth, compared to 68% from CaCl2 and 35–75% from Ca fulvates. Correspondingly, CaCO3 precipitated all KCl-extractable Al in the top 5 cm, but had no effect beyond the 10-cm depth. The CaCl2 displaced a small but significant portion of extractable Al from the top 15 cm and redeposited some of that Al in lower depths. Similar to CaCO3, Ca fulvates from chicken manure and green mamure only decreased extractable Al significantly in the top 10-cm layers, but had little effect beyond that depth. By contrast, the Ca fulvate from sewage sludge decreased Al down to the 45-cm depth. In terms of reducing Al saturation as a percentage of total extractable cations (effective cation exchange capacity), the Ca fulvates were as effective as CaCO3 in the 0-to 5-cm layer, and more effective than CaCl2 in any soil layer because of the increased exchangeable Ca and/or decreased Al. In general, surface application of common organic material-derived Ca fulvates can increase subsoil Ca and decrease the Al saturation percentage. However, Mg depletion and enrichment of unwanted metals (e.g., Na or heavy metals) may be a problem when leaching with these organic sources. 相似文献
26.
钱桦 《安徽农业大学学报》2004,(6):130-132
分析全球化对我国文化建设的影响及图书馆而临的机遇和挑战,提出在全球化的背景下,我国图书馆改革和发展的对策。 相似文献
27.
Determination of genetic variability by RAPD markers in cauliflower,cabbage and kale local cultivars from France 总被引:3,自引:1,他引:3
RAPD markers were used to analyse the genetic variability among a cole crop cultivar collection from France and to identify
possible duplicate accessions for facilitating the germplasm multiplication. We surveyed 24 cauliflower, 24 cabbage and 48
kale populations using 62, 100 and 89 RAPD markers respectively on 40 seed bulk samples per accession. Genetic distances were
calculated on the basis of these markers. Using the phylogenetic package PAUP we compared the genetic clusters obtained for
the RAPD markers with the groups based on morphologic and agronomic data. For cauliflowers and cabbages a drastic selection
by growers led to an extensive differentiation of morphologic and precocity traits related to geographic origin, which matches
the grouping based on RAPD markers. Furthermore, RAPDs detected in some cases misclassification of accessions in the collection.
Kales formed an homogeneous group probably due to extensive genetic exchanges leading to a continuous diversity. For the other
crops, different horticultural types were separated in several subgroups by the RAPD markers suggesting different genetic
origins. RAPD markers provide a rapid and informative approach to analyze the genetic variability in a collection. It can
be applied to autogamous (spring cauliflower) or allogamous (winter cauliflower) crops, even in cases where the intra-population
diversity is extensive (cabbages). 相似文献
28.
Sewage sludges from six wastewater treatment plants in Hawaii were periodically sampled to determine the seasonal variation of their elemental composition. The Sand Island and Honouliuli treatment plants produced primary sludges averaging 1.6% and 2.0% total N, respectively. The Kailua, Kaneohe, Waimanalo, and Waianae treatment plants produced secondary sludges averaging 5.4, 5.1, 6.1, and 3.5% N, respectively. All the sludges tested were virtually devoid of K with concentration ranging from 0.01 to 0.15%, which was less than half of the 0.30% K considered typical for a US sewage sludge. Mean concentrations of Cd, Cu, Fe, Ni, and Zn in the Hawaii sludges were 5.9, 373, 12343, 218, 36.7, and 817 mg kg?1, respectively, which were within the norms for sludge heavy metals as reported by the US Environmental Protection Agency. Seasonal variations in elemental concentration were small and only statistically significant for Ca and Zn. Sludges from the three treatment plants with highest annual production (Sand Island, Honouliuli, and Kailua) were then mixed at 5, 50, and 250 g kg?1 with three representative tropical soils (a Mollisol, and Oxisol, and an Ultisol) to study sludge-soil reactions and plant responses. Soil-solution data indicated that chemical properties of a sludge-soil mixture depended not only on the soil, sludge, and its application rate, but also on sludge-soil interactions. At an agricultural rate of 5 g kg?1 (10 Mg ha?1), the anaerobically digested Kailua sludge increased corn (Zea mays L.) biomass, whereas the two undigested sludges reduced it. At higher rates, Mn phytotoxicity resulted from sludge applications to the Mollisol and Oxisol, both of which contained reducible Mn nodules. Significant growth reductions would be expected when corn seedlings contained ≥200 mg Mn kg?1 or ≤0.30 % Ca; and, adequate supplies of Ca and Zn seemed to lessen Mn phytotoxicity. 相似文献
29.
Cavazzana-Calvo M Hacein-Bey S de Saint Basile G Gross F Yvon E Nusbaum P Selz F Hue C Certain S Casanova JL Bousso P Deist FL Fischer A 《Science (New York, N.Y.)》2000,288(5466):669-672
Severe combined immunodeficiency-X1 (SCID-X1) is an X-linked inherited disorder characterized by an early block in T and natural killer (NK) lymphocyte differentiation. This block is caused by mutations of the gene encoding the gammac cytokine receptor subunit of interleukin-2, -4, -7, -9, and -15 receptors, which participates in the delivery of growth, survival, and differentiation signals to early lymphoid progenitors. After preclinical studies, a gene therapy trial for SCID-X1 was initiated, based on the use of complementary DNA containing a defective gammac Moloney retrovirus-derived vector and ex vivo infection of CD34+ cells. After a 10-month follow-up period, gammac transgene-expressing T and NK cells were detected in two patients. T, B, and NK cell counts and function, including antigen-specific responses, were comparable to those of age-matched controls. Thus, gene therapy was able to provide full correction of disease phenotype and, hence, clinical benefit. 相似文献
30.
Sixteen composts consisting of 14 commercial samples, one immature yard waste compost and one raw material of mostly grass clippings were evaluated for their stability. In a three-day incubation test, the commercial composts yielded from 9 to 99 mg CO2/kg/ hr, with a mean CO2 production rate of 61 mg CO2/kg/hr. By contrast, the immature compost produced 684 mg CO2/kg/hr and the raw material, 1,433. The low CO2 production rates of the commercial composts along with dark brown color and lack of unpleasant odors in moist conditions indicated that these composts were indeed stable. We devised a quick chemical test to predict compost stability. Several compost properties were measured: (i) total (C, N and C:N), (ii) water-soluble (C, N and C:N), (iii) NaOH-soluble C, humic and fulvic acids, and optical absorbances at 465 nm (E4) and 665 nm (E6) of the NaOH-soluble fractions. Water-soluble fraction, particularly water-soluble C and the C:N ratio, best separated stable from unfinished composts. However, water-soluble organic N in some stable composts was less than 0.01 g/kg, making measurement difficult. Thus, alternatively a ratio of water-soluble C:total organic N ≤0.70, based on Mean (0.32) + 2*SD (0.19), is suggested as a predictor of compost stability. Also, compost stability can be predicted by NaOH-soluble C:water-soluble C ≥6.0, but not by (humic acid:fulvic acid) or (E4:E6) of any NaOH-soluble fractions. 相似文献