Modeling and Its Application of Reinforced Concrete Columns Considering Nonlinear Shear Effects

A fiber beam-column element in conjunction with zero-length elements attached to its ends was proposed to simulate the flexural and shear mechanism respectively. Based on the Limit State Material model and the Shear Limit Curve model provided by OpenSees, the nonlinear shear effect of reinforced concrete column and its coupling with the flexural effect were defined. The reliability of the proposed model was validated by means of comparisons with existing test results. Finally, a plane frame from in-situ pushover test was simulated. It is shown that the proposed method, by taking the nonlinear shear effect into account, produces satisfactory results for frame columns with shear strength and stiffness degradation, while the conventional fiber beam-column element can hardly simulate actual flexure-shear failure mechanism for columns characterized by insufficient transverse reinforcement. The proposed method is applicable for nonlinear analysis of reinforced concrete frame structures with shear deficiencies.     

水平荷载作用下斜坡刚性桩非线性分析

在综合分析现有水平荷载作用下桩基分析方法的基础上,建立了考虑桩侧土体受力状态的斜坡刚性桩力学模型;根据极限平衡原理,建立横向荷载作用下斜坡刚性桩弯矩和应力平衡方程;引入考虑斜坡影响的p-y曲线方法,提出了综合考虑桩侧土体极限承载力与水平抗力系数沿深度呈线性增加的侧向极限承载力与土体抗力承载力系数计算方法,同时,将该方法应用于计算实例,通过与已有有限元和理论计算方法对比分析,计算结果验证了本文方法的合理性与可行性;并利用该方法,分析了斜坡坡角、桩土接触面系数以及地基水平抗力系数对斜坡刚性桩承载特性的影响因素。分析表明:斜坡的坡角、桩土接触面系数对侧向荷载作用下斜坡刚性桩的荷载位移曲线影响明显,而桩侧土的抗力系数对侧向荷载作用下斜坡刚性桩的荷载位移曲线影响不明显。     

Genetic analysis and fine mapping of a semi-dwarf gene in a centromeric region in rice (Oryza sativa L.)

Superior plant architecture is a key means of enhancing yield potential in high yielding varieties. A newly identified recessive gene, named sd-c, controls plant height and tiller number. Genetic analysis of an F₂ population from a cross between the semi-dwarf mutant and japonica cv. Houshengheng showed that the sd-c locus was flanked by SSR markers RM27877 and RM277 on chromosome 12. Thirty nine InDel markers were developed in the region and the sd-c gene was further mapped to a 1 cM centromeric region between InDel markers C11 and C12. These sequenced markers can be used to distinguish wild type and mutants and thus can be used in marker-assisted selection. The sd-c mutant decreases culm length by about 26% and doubles the tiller number without changing seed weight. Until now only sd-1 has been used in indica rice breeding programs. The sd-c mutant seems to have no undesirable pleiotropic effects and is therefore a potential genetic resource for breeding semi-dwarf indica rice cultivars.     

沂河流域土壤侵蚀分布特征研究

为探索沂河流域土壤侵蚀的强度和规律,利用ArcGIS的空间分析与统计分析功能,从高程、坡度、土壤类型、土地利用4个方面,对沂河流域土壤侵蚀的空间分布特征进行研究。结果表明：（1）流域土壤侵蚀总体以中度侵蚀为主,存在较为明显的区域差异;（2）侵蚀主要发生在600 m以下区域,其中高程位于200~400 m之间的区域侵蚀面积最大;（3）5°以下以及8°~15°坡度级土壤侵蚀面积较大,土壤侵蚀随坡度的增加总体都呈现出先增强后减弱的趋势;（4）土壤总面积越大,侵蚀面积也越大。石质土、粗骨土的土壤侵蚀率较高,潮土和砂姜黑土的土壤侵蚀率较低;（5）耕地、草地和林地侵蚀面积最大,林地、草地、未利用地土壤侵蚀率较大,水域土壤侵蚀率最小。     

不同移栽时期对山西早熟区春玉米生长及产量的影响

为了探索在山西早熟区无膜种植玉米的新模式及明确该地区玉米育苗移栽的适宜移栽时期,采用完全随机区组设计大田试验,以覆膜直播为对照,分析比较了不同时期露地移栽对玉米生育进程、株高、成熟期棒三叶叶面积与产量及其构成因素的影响。结果表明:各时期移栽玉米的生育进程均慢于对照。移栽具有降低株高与减小成熟期棒三叶叶面积的效应。在本试验条件下5月1日移栽玉米产量与对照无显著差异,较覆膜直播(CK)仅减少了7%。在早熟地区采用育苗移栽方法无膜种植玉米时必须注意当地晚霜对玉米幼苗的伤害,可以认为在该地区适宜的移栽时期应当是在当地晚霜之后抓紧时间尽早移栽。     

增氧对水培棉花生长的影响研究

通过水培试验研究增氧对棉花生长的影响;试验设计3种增氧方式,分别为持续性增氧(Physical continuous oxygen,PCO)、间歇性增氧(Physical oxygen,PO),化学增氧(Chemical oxygen,CO),种棉花不增氧CK,不种棉花不增氧CK0;研究结果表明,不同增氧方式对营养液中溶解氧浓度都有不同程度的提高,表现为PCOPOCOCK;PCO处理对根体积、根系总吸收面积、根活性面积、根系生物量、株高、地上部生物量、氮和钾的吸收量的促进作用均达到显著水平,分别比CK增加了194.62%、261.89%、301.73%、57.15%、22.76%、38.03%、35.27%、84.78%;间歇性增氧PO处理对根系生物量、株高、地上部分生物量的促进作用显著,分别比CK高30.83%、15.65%、21.19%。通过以上结果表明,棉花对氧的需求敏感,不同增氧方式均能在一定程度上促进棉花生长,其中持续性增氧效果尤为突出,特别是对棉花生物量的积累,说明增氧可以提升棉花生长潜力。     

Mapping and candidate gene analysis for a new top spikelet abortion mutant in rice

Grain numbers is one of the determinations for rice yield and directly associated with spikelet numbers per panicle and its normal development. Lots of genes responsible for spikelet numbers and spikelet early development have been identified, but the molecular information about the spikelet development at later development is still limited. Here, we isolated a rice spikelet abnormal development mutant, which shows degenerated spikelet at the top panicle and named aborted top spikelet mutant 1(Ats1). The spikelets derived from the middle and bottom branches per panicle of Ats1 show normal development with those of wild type. However, a large number of branches and spikelets with arrested development were often observed only on apex panicle. The abnormality did not appear until the stage In8 when rachises elongate rapidly and reproductive organs get mature, based on observations through SEM analysis. The aborted spikelet could develop the complete floral organs with a pair of rudimentary glume, a pair of empty glume, two lodicule, six stamens and one carpel. But all these floral organs did not develop maturity. Genetic analysis on two F₂ populations indicated that the Ats1 was controlled by a single dominant gene. By using bulked segregant analysis of F₂ population developed from Ats1 crossing with Songjing6, ATS1 was mapped on chromosome 8 between RM3819 and RM5556. Then, the fine mapping was performed with 1078 F₂ population developed from Ats1 and IR36. The ATS1 locus was finely mapped in an 85.7 kb region between RM22448 and STS8‐2 with 8 genes according to the rice annotation project database. Sequence analysis of the candidate genes within the delimited region of the Ats1 and Akihikari showed two‐nucleotide changes, including single‐nucleotide substitutions corresponding to an amino acid substitution from asparagine to lysine acid in exons 3 and a 1‐bp deletion resulting in a premature stop codon in exon 22 at the candidate gene, LOC_Os08g06480. A cleaved amplified polymorphic sequence (CAPS) marker, CAPS‐ats1, was developed from the 1‐bp deletion site. The complete cosegregation of the CAPS genotypes with the matching phenotypes were observed in the F₂ populations. This suggested that Os08g06480 is most likely the ATS1 gene. These results will provide more information for better understanding of the molecular mechanism governing top spikelet abortion within a short developmental period.     

Application of marker‐assisted backcross to introgress Bph3, Bph14 and Bph15 into an elite indica rice variety for improving its resistance to brown planthopper

To improve brown planthopper (Nilaparvata lugens Stål; BPH) resistance of an elite indica cultivar of South China, Hemeizhan (HMZ), we applied marker‐assisted backcross (MABC) to incorporate three BPH‐resistance genes (Bph3, Bph14 and Bph15) into the genetic background of HMZ. In the third backcross (BC₃) generation, we obtained near‐isogenic lines (Bph3‐NIL, Bph14‐NIL, Bph15‐NIL and Bph14 + Bph15‐NIL) with more than 96% recovery of recurrent parent genome, and pyramided lines (Bph3 + Bph14‐PYL, Bph3 + Bph15‐PYL and Bph3 + Bph14 + Bph15‐PYL) with more than 89% recovery of recurrent parent genome. These lines showed stronger resistance against BPH than HMZ at seedling and booting stages. The rank of resistance gene effect was Bph3 + Bph14 + Bph15 ≥ Bph3 + Bph15 ≥ Bph3 +Bph14 ≥ Bph14 + Bph15 ≥ Bph3 ≥ Bph15 ≥ Bph14 > none. Compared with HMZ, only Bph3 + Bph14 + Bph15‐PYL had a significant difference in yield per plant, and the lines carrying Bph3 had higher amylose contents, indicating that Bph3 was tightly linked to Wx^a allele. These improved lines are good intermediate sources of broad‐spectrum and durable BPH resistance to improve other indica cultivars. Our results demonstrate that MABC is a very efficient approach to improve BPH resistance of elite rice cultivar.     

野生大麦的抗旱性及其在大麦遗传改良中的应用

干旱是主要的非生物胁迫性灾害,极大地威胁到全球的粮食供应,是影响农业生产的最重要因素之一。因此,培育抗旱性植物是解决干旱危害和粮食危机的根本途径。野生大麦已经适应广泛的干旱环境并具有丰富的遗传多样性,它们的抗旱基因与抗旱相关数量性状位点(QTLs)已经确定,对改良大麦抗旱性有巨大的潜力。综述了野生大麦的抗旱性及其在大麦改良中的应用,并对大麦抗旱育种中存在的问题以及今后发展方向进行了展望。     

奥利亚罗非鱼在光场中的行为反应研究

为探索了解奥利亚罗非鱼在光场中的趋避行为规律。采用水平光梯度法,研究了4组不同生长阶段的奥利亚罗非鱼：Ⅰ组：20.0~50.0 mm;Ⅱ组：50.0~80.0 mm;Ⅲ组：80.0~110.0 mm;Ⅳ组：110.0~140.0 mm,在250、500、1000、1500、2000 lx白光光强梯度下的行为反应。同时对Ⅰ组奥利亚罗非鱼在不同波长单色光下（红、黄、绿、蓝）下的行为分布开展研究。结果表明：在白光下,4组奥利亚罗非鱼均表现出正趋光性,在250~2000 lx间趋光率随光照强度的增强呈现出先上升后下降的趋势,在1000 lx时趋光率达到峰值,其中近光区趋光率分别为67.5%、55.0%、55.0%、42.0%,总趋光率分别为96.0%、94.0%、96.5%、95.0%;奥利亚罗非鱼的白光适宜照度范围为：500~1500 lx;随着奥利亚罗非鱼的生长发育,近光区趋光率峰值Ⅰ>Ⅱ>Ⅲ>Ⅳ。此外,Ⅰ组奥利亚罗非鱼在不同单色光同一照度下的趋光率差异显著(P<0.05),在蓝光、绿光下的趋光率高于红光、黄光,对不同单色光的趋集行为具有选择性。研究表明：奥利亚罗非鱼在不同光色、不同光强、不同生长阶段都具有选择性,研究结果在开发高效诱捕灯、提高工厂化养殖效益等方面具有积极意义。