养殖场环境控制浅析

近20多年来,我国养殖业发展迅速,取得了可喜的业绩。我国肉、蛋、禽总产量连续保持世界第一,为改善我国人民生活水平,调整人们膳     

早熟紫长茄新品种玫茄1号的选育

玫茄１号的母本是从台湾紫长茄中选育出的自交系２１－３,父本是从福建地方品种红茄中选育出的自交系２１－４,其杂种一代表现早熟,比对照闽茄１号早收７ｄ（天）,果长３０～３３ｃｍ,稍弯,果径４ｃｍ,单果重１８０ｇ,果皮鲜紫色,果肉乳白色,肉质细嫩,商品性好。一般每公顷产３０～４５ｔ,比对照增产１９．５％。该品种已在闽、浙、赣、粤等省推广,种植面积达３２００ｈｍ２。     

抗菌肽基因导入桑树获得抗病转基因植株

用携带抗菌肽基因的农杆菌处理桑子叶,在含有羧苄青霉素（Cb）400～500mg／L和卡那霉素（Km）20mg／L的MS培养基上,有32.4％的子叶产生了不定芽;66.5％的不定芽在含有Cb300mg／L和Km30～40mg／L的培养基中,正常生长成2～3cm高的新稍;新梢在含Cb50mg／L和Km10mg／L的生根培养基中有72．6％形成完整根系。3次转化共获得12个株系55株KmR植株。不同株系桑苗叶片DNA点杂交分析显示,7个株系有阳性杂交信号。KmR株系桑苗的抗病性测定显示,5个株系共14株桑苗对青枯病具有较强抗性.     

211省道金川县庆宁至河西段绿化设计理念初探

211省道庆宁至河西段绿化景观设计以“美丽金川、百里画廊”为总体设计理念,其设计理念主要体现在公路的5个主题景观段落中,每个段落确定一个主题和要表达到的意境,按照“梨花春雪三月天,桃红柳绿似江南.一段一景皆有意,百里画廊入梦来.”的设计构思,把公路打造成集塞上江南、高原梨乡、地方文化特色和魅力为一体蕴含丰富景观的风景画卷,以达到丰富公路景观效果,提升公路的绿化档次的目的.本段公路绿化景观化设计为省级公路的绿化设计及建设开创了一个新的思路.     

AX=b在逆M-阵类中的反问题

将线性方程组 Ａ Ｘ＝ ｂ 分为５ 种情况（ Ｘ＞ ０、 Ｘ≥０、 Ｘ＜ ０、 Ｘ≤０、 Ｘ 为一般情况），通过构造矩阵的方法，讨论了该线性方程组的反问题在逆 Ｍ－ 阵类中有解的条件．在一般情况下，当给定的实向量 Ｘ 与ｂ 中相应的分量同号时，则线性方程组 Ａ Ｘ＝ ｂ 在逆 Ｍ － 阵类中的反问题有解．     

高山草原绵羊放牧生态及消化代谢的研究：Ⅰ放牧绵羊嗜食性研究

利用植物显微技术镜检法和食道瘘管法对放牧绵羊采食的高山草原植物成分与嗜食性指数进行分析。结果表明，绵羊在黄草期采食植物成分比青草期多，其嗜食性指数在不同时期变幅较大，随着冬季来临，绵羊对草地植物的嗜食性不如青草期强烈，至某些植物嗜食性是相对稳定的。     

淡水环境中可溶有机质研究进展

论述了淡水环境中可溶有机质(DOM)在分离与分析方法、来源与特征,以及环境意义等方面的研究进展。指出了在分离方法上,从XAD树脂分离到反渗透膜分离已经发展出各种分离技术;在分析方法上,现代波谱分析技术得到了广泛应用。DOM是由在来源、年龄、存在状态、后生改造等方面存在巨大差异的不同有机质混合构成的,因而呈现出十分复杂的非均质结构。DOM通过其自身的生物可利用性、改变有机污染物和重金属的生物可利用性及其耗氧能力影响环境质量,而且其自身的一些地球化学特征也能用来反映环境的变化特点。最后简述了湿地淡水环境中DOM的研究趋势和展望。     

2007年11月全国饲料生产情况分析

1生产总况 2007年11月，国内饲料生产形势有所好转。进入11月国内大部分地区的气温明显下降，促进了肉类的消费。而生猪价格已经接近甚至超过了7、8月份的最高水平。从消费方面看，已经进入元旦、春节消费高峰期，后期市场需求将增加，养殖户补栏积极。大体重的仔猪尤其抢手。在猪市旺盛的带动下，其他畜产品市场也水涨船高，除水产料产量下降外，其他品种饲料均呈现出产销两旺的可喜局面。     

Influence of murine cytomegalovirus infection on the differentiation and the differentiation genes expression in neural stem cells

AIM: The influence of MCMV infection on differentiation and differentiation gene expression in neural stem cells(NSCs) in vitro were investigated for studying the mechanisms of brain abnormalities caused by congenital cytomegalovirus infection.METHODS: NSCs were separated from fetal BALB/C mouse, and cultured and identified in vitro. The differentiation potency of NSCs was observed by immunofluorescence. The NSCs infected by MCMV at dosage of MOI(multiplicity of infection) equaled to 5, 1 and 0.1,respectively, were cultured in differentiation medium. The morphological changes of infected cells were observed under inverted microscope. The ratios of NSCs and its differentiated cells were detected by flow cytometry. The expressions of nestin, GFAP and NSE, markers of NSCs and its differentiated cells, were studied by immunofluorescence(MOI=1). The expression of early antigen(EA) of MCMV was detected to observe the infection process. Real-time RT-PCR method was employed to measure the expression levels of the key genes Neurog2, Myc and Ccnd1 in Wnt signal pathway of NSCs at early stage of differentiation culture.RESULTS: NSCs isolated from embryonic mouse brains proliferated to form neurospheres, strongly expressed nestin and differentiated into NF-200 positive neurons or GFAP positive astrocytes. The infected NSCs did not adhere to the wall and appeared differentiation growths, but showed swollen gradually after differentiation culture. The nestin expression in the infected cells downregulated slowly and was higher than that in control groups(P<0.05). The GFAP and NSE expressions of the infected cells were lower than those in control groups(P<0.05). The early antigen(EA) of MCMV was always detected in the cells in infected groups. The ratios of nestin positive cells in infected groups were higher than those in control groups, but the ratios of GFAP and NSE positive cells of former were lower than that of the latter from 3rd to 9th d after differentiation culture(P<0.05). The levels of Neurog2 mRNA and Myc mRNA in infected groups were markedly lower than those in normal control groups on 1st d and from 1st to 4th d after differentiation culture, respectively(P<0.05). The levels of Ccnd1 mRNA of infected groups were obviously lower than those in normal control groups from 12th h to 1st d(P<0.05). These changes in infected groups became more obvious as MCMV MOI increased.CONCLUSION: MCMV significantly inhibits differentiation of NSCs to neurons and astrocytes, and leads to the decrease in differentiated cells. MCMV inhibits or interferes with the gene expression of Neurog2, Myc and Ccnd1 in Wnt signal pathway of NSCs. The effect that MCMV inhibits the expressions of differentiation and the differentiation genes in NSCs shows dose-dependent with MCMV MOI. The inhibitory effect of MCMV on the differentiation of NSCs might be induced by interfering with the expression of differentiation gene in NSCs, which is possibly the one of primary causes of brain development disorders induced by congenital CMV infection.     

汉源县云南松成熟林和过熟林的更新树种和更新模式初探

汉源县云南松林是20世纪60年代中期至70年代初期由飞机播种和人工点播造林而成的林分，成林林分多为纯林，现已进入成熟林期，林木大部分生长较弱或已停止生长，舞毒蛾、松梢螟、松梢小卷蛾、松纵坑切梢小蠹虫等病虫均侵入危害，林分进入更新周期。找到切台汉源实际且行之有效的更新模式和更新树种十分重要。