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81.
Hak Jin Kim Yong Woo Kim In Sook Lee Jong Woon Song Yeon Joo Jeong Seon Hee Choi Kyung Un Choi Kuen Tak Suh Byung Mann Cho 《Acta veterinaria Scandinavica》2009,51(1):30
Background
To test the hypothesis that triolein emulsion will increase vascular permeability of skeletal muscle.Methods
Triolein emulsion was infused into the superficial femoral artery in rabbits (triolein group, n = 12). As a control, saline was infused (saline group, n = 18). Pre- and post-contrast T1-weighted MR images were obtained two hours after infusion. The MR images were qualitatively and quantitatively evaluated by assessing the contrast enhancement of the ipsilateral muscles. Histologic examination was performed in all rabbits.Results
The ipsilateral muscles of the rabbits in the triolein group showed contrast enhancement, as opposed to in the ipsilateral muscles of the rabbits in the saline group. The contrast enhancement of the lesions was statistically significant (p < 0.001). Histologic findings showed that most examination areas of the triolein and saline groups had a normal appearance.Conclusion
Rabbit thigh muscle revealed significantly increased vascular permeability with triolein emulsion; this was clearly demonstrated on the postcontrast MR images. 相似文献82.
Jin-Joo Hue Ki Nam Lee Jae-Hwang Jeong Sang-Hwa Lee Young Ho Lee Seong-woon Jeong Sang Yoon Nam Young Won Yun Beom Jun Lee 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(3):189-195
This study was to investigate the anti-obesity effects of diglyceride (DG)-conjugated linoleic acid (CLA) containing 22% CLA as fatty acids in C57BL/6J ob/ob male mice. There were four experimental groups including vehicle control, DG, CLA, and DG-CLA. The test solutions of 750 mg/kg dose were orally administered to the mice everyday for 5 weeks. CLA treatments significantly decreased mean body weight in the obese mice throughout the experimental period compared to the control (p < 0.01). All test solutions significantly decreased the levels of triglyceride, glucose and free fatty acids in the serum compared with control (p < 0.05). The levels of total cholesterol were also significantly reduced in DG and DG-CLA groups compared with the control group (p < 0.05). CLA significantly decreased weights of renal and epididymal fats compared with the control (p < 0.05). DG and DG-CLA also significantly decreased the epididymal fat weights compared with the control (p < 0.05). A remarkable decrease in the number of lipid droplets and fat globules was observed in the livers of mice treated with DG, CLA, and DG-CLA compared to control. Treatments of DG and CLA actually increased the expression of peroxisome proliferator-activated receptor gamma. These results suggest that DG-CLA containing 22% CLA have a respectable anti-obesity effect by controlling serum lipids and fat metabolism. 相似文献
83.
Hye Kwon Kim Jeong Sun Yang Hyoung Joon Moon Seong Jun Park Yuzi Luo Chul Seung Lee Dae Sub Song Bo Kyu Kang Soo Kyung Ann Chan Hyuk Jun Bong Kyun Park 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(2):121-130
The 23 open reading frame (ORF) 5 sequences of Korean type II porcine reproductive and respiratory syndrome virus (PRRSV) were collected from viremic sera from the (modified live vaccine) MLV-vaccinating and non-vaccinating farms from 2007 to 2008. The samples were phylogenetically analyzed with previous ORF5 sequences, including type I Korean PRRSV, and previously reported or collected sequences from 1997 to 2008. A MN184-like subgroup of type II Korean PRRSV was newly identified in the viremic sera collected from 2007 to 2008. And of the type I PRRSVs, one subgroup had 87.2~88.9% similarity with the Lelystad virus, showing a close relationship with the 27~2003 strain of Spain. The maximum parsimony tree of type II PRRSV from 1997 to 2008 showed that they had evolved to four lineages, subgroups 1, 2, 3 and 4. Most of the recently collected type II PRRSVs belonged to subgroup 4 (48%). The region of three B-cell epitopes and two T-cell epitopes of ORF5 amino acids sequences was considerably different from the MLV in subgroups 3 and 4. In conclusion, the existence of type I PRRSV, which was genetically different from Lelystad virus (Prototype of type I PRRSV), and heterologous type II PRRSVs of viremic pigs detected even in the MLV-vaccinating farms indicated the need for new vaccine approaches for the control of PRRSV in Korea. 相似文献
84.
Dae Sub Song Youn Jeong Lee Ok Mi Jeong Yong Joo Kim Chan Hee Park Jung Eun Yoo Woo Jin Jeon Jun Hun Kwon Gun Woo Ha Bo Kyu Kang Chul Seung Lee Hye Kwon Kim Byeong Yeal Jung Jae Hong Kim Jin Sik Oh 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(4):323-329
Active serologic surveillance is necessary to control the spread of the avian influenza virus (AIV). In this study, we evaluated a commercially-available cELISA in terms of its ability to detect AIV antibodies in the sera of 3,358 animals from twelve species. cELISA detected antibodies against reference H1- through H15-subtype AIV strains without cross reactivity. Furthermore, the cELISA was able to detect antibodies produced following a challenge of the AIV H9N2 subtype in chickens, or following vaccination of the AIV H9 or H5 subtypes in chickens, ducks and geese. Next, we tested the sensitivity and specificity of the cELISA with sera from twelve different animal species, and compared these results with those obtained by the hemagglutination-inhibition (HI) test, the "gold standard" in AIV sera surveillance, a second commercially-available cELISA (IZS ELISA), or the agar gel precipitation (AGP) test. Compared with the HI test, the sensitivities and specificities of cELISA were 95% and 96% in chicken, 86% and 88% in duck, 97% and 100% in turkey, 100% and 87% in goose, and 91% and 97% in swine, respectively. The sensitivities and specificities of the cELISA in this study were higher than those of IZS ELISA for the duck, turkey, goose, and grey partridge sera samples. The results of AGP test against duck and turkey sera also showed significant correlation with the results of cELISA (R-value >0.9). In terms of flock sensitivity, the cELISA correlated better with the HI test than with commercially-available indirect ELISAs, with 100% flock sensitivity. 相似文献
85.
Krishna Hari Dhakal Yeon-Shin Jeong Jeong-Dong Lee In-Youl Baek Tae-Joung Ha Young-Hyun Hwang 《Journal of Crop Science and Biotechnology》2009,12(2):97-101
Fatty acid composition and C18:2:C18:3 of soybean seed and sprout is important for soy-industries to manufacture quality products. Six soybean varieties were analyzed
by gas chromatography to determine fatty acid composition and linoleic to linolenic acid ratio (C18:2:C18:3) in seed, sprout, and their structural parts. In the case of whole seed and its structural parts, significant variation in
fatty acid composition and C18:2:C18:3 were observed between varieties except palmitic acid (C16:0), while all the parameters were significantly different for seed parts. Significant interactions of variety with seed parts
were observed for oleic acid (C18:1), C18:2 and C18:2:C18:3. The highest saturated fatty acid, C18:1 and polyunsaturated fatty acid were recorded in seed coat, cotyledon, and seed axis, respectively. The lowest C18:2:C18:3 was found in seed axis. In the case of sprout study, variety had significant effect on all the parameters observed for whole
sprout, cotyledon, root, and except C18:3 for hypocotyl. Culture days had significant effect on C16:0, C18:2 and C18:3 in whole sprouts, while only on C18:3 and C18:2:C18:3 in cotyledon. For hypocotyl, culture days had significant effect on C18:1, C18:2 and C18:2:C18:3, however, C16:0, C18:1, C18:3, and C18:2:C18:3 were significantly different in root. In sprout, days and variety interacted significantly for C16:0, C18:3 and C18:2:C18:3, and C18:1, C18:2, C18:3, and C18:2:C18:3, respectively for whole, cotyledon, and root. The significantly lowest C18:2:C18:3 (1.1) was observed in hypocotyl and root of Hwangkeumkong in 5 days. Knowledge of fatty acid composition and C18:2:C18:3 of seed, sprout, and their parts could be applicable for oil and other soy-food industries to make quality products. 相似文献
86.
Kim DK Jeong SC Gorinstein S Chon SU 《Plant foods for human nutrition (Dordrecht, Netherlands)》2012,67(1):71-75
The aim of this investigation was to evaluate the biological, alcohol dehydrogenase (ADH) and antiproliferative activities
of different extracts of mungbean seeds and sprouts. All extracts from the sprouts showed higher contents of total phenolics
(TP), total flavonoids (TF), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than from seeds. The highest
DPPH and tyrosinase inhibition activities were registered in ethyl acetate (EtOAc) extract. ADH activity of methanol (MeOH),
n-hexane (n-hexane) and n-butanol (n-BuOH) extracts from sprouts was significantly higher (P < 0.05) than from seeds. However, the highest ADH activity was found in water extract of seeds. According to 3-(4, 5-dimethylthiazol
-2-yl)-2, 5-diphenyltetrazolium bromide) (MTT) assay, extracts from sprouts were more effective against Calu-6 (human pulmonary
carcinoma) and SNU-601 (human gastric carcinoma) cells than from seeds. EtOAc extract showed the highest antiproliferative
activity in both sprouts and seeds, followed by n-hexane extraction. During sprouting of mungbean, all the studied components
significantly increased. In conclusion, the extracts of sprouts are more effective than from seeds and could be a potential
source of antioxidants linked with health benefits. 相似文献
87.
Silsesquiazane (SSQZ), one of the branched forms of polysilazane, was blended with polystyrene (PS), poly(methyl methacrylate) (PMMA), poly(iso-butyl methacrylate) (PBMA), and poly(vinyl acetate) (PVAc) to produce organic-inorganic hybrid materials. The polymers were selected to provide specific interaction, such as ??-?? interaction or hydrogen bonding, with SSQZ. AFM studies revealed that carbonyl containing polymers, PMMA, PBMA, and PVAc were miscible with SSQZ while PS was immiscible. DSC analysis of the hybrids also supported the miscibility behaviours of the system. The glass transition behaviour of SSQZ/PS showed individual transition temperature corresponding to the each component while SSQZ/carbonyl-containing polymers displayed one transition temperature that matched with the theoretically calculated values following the Kwei equation. Among the miscible hybrid system, the interaction with SSQZ increased in the order of PMMA> PBMA> PVAc, as determined by the q value in the Kwei equation. This result was confirmed by determining the degree of hydrogen bonding in FT-IR spectra. Thermal stability of organic polymers was improved by incorporating SSQZ regardless of the miscibility. 相似文献
88.
Moon-Young Kim Jae-Jun Ahn Kashif Akram Gui-Ran Kim Mi-Seon Jeong Ji-Young Kwak 《Journal Of Aquatic Food Product Technology》2013,22(3):299-306
ABSTRACTRadiation-specific luminescence properties in irradiated dried-fishery products (pollack, little pollack, clams, and shrimp) were investigated at different dose levels (0–10 kGy). Photostimulated luminescence (PSL) analysis of whole samples was effective for dried clams and shrimp, while low PSL sensitivity was observed in dried pollack and little pollack. Thermoluminescence (TL) analysis was conducted after isolation of silicate minerals, in which the two methods of mineral isolation—density separation and acid hydrolysis—were compared. Irradiated samples were easily distinguishable through the strong TL glow curves, with maximum peaks in temperature range of 150–250°C. TL ratios (TL1/TL2) were < 0.1 for nonirradiated samples, while > 0.1 for irradiated. There was a clear effect of applied irradiation dose, with a negligible effect of the method used for mineral separation. The mineral composition showed that feldspar and quartz minerals were mainly responsible for the well-characterized luminescence behavior upon irradiation. 相似文献
89.
Salinity changes in the anadromous river pufferfish,Takifugu obscurus,mediate gene regulation 总被引:1,自引:0,他引:1
Su-Young Jeong Jin-Hyoung Kim Wan-Ok Lee Hans-Uwe Dahms Kyung-Nam Han 《Fish physiology and biochemistry》2014,40(1):205-219
This study aimed to better understand the hydromineral regulatory response of the anadromous river pufferfish, Takifugu obscurus, to salinity changes through real-time RT-PCR. After abrupt transfer from 30 or 5 psu to 5 or 30 psu, respectively, we analyzed the mRNA expression of Na+/K+ ATPase, prolactin receptor, and aquaporin from osmoregulatory organs of the river pufferfish such as gills, kidney, and intestine. Na+/K+ ATPase showed notable changes in the gills and kidney when salinity was increased. In the gills, the expression level of Na+/K+ ATPase suddenly increased within a day after abrupt transfer from 5 to 30 psu and then slightly declined within 2 days after exposure. In the kidney, Na+/K+ ATPase has shown consistently high mRNA expression after the increase in salinity. Expression levels of the prolactin receptor gene increased when environmental salinity decreased. In the intestine, gene expression of the prolactin receptor remained high, even when salinity decreased. To the contrary, there was a steady increase or decrease in mRNA expression in the kidney in response to salinity decrease or increase, respectively. As for aquaporins, aquaporin 1 was mainly expressed in the intestine and kidney, and aquaporin 3 was mainly expressed in the gills and intestine. In the gills, increased expression of aquaporin 3 was found after transfer to lower salinity and in the intestine and kidney, a decrease in salinity followed by an abrupt decrease in aquaporin 1 and aquaporin 3. Contrastingly, the expression of these genes increased in the intestine after transfer to 30 psu. Osmoregulatory genes were expressed in diverse organs, apparently to overcome an influx or exhaust of water or ions. A superior adaptation ability of the river pufferfish to a wide range of salinities is most reasonably due to active osmoregulatory processes mediated by the genes monitored here. 相似文献
90.
Hee Ock Boo Jeong Hun Park Hag Hyun Kim Soo Jeong Kwon Sun Hee Woo 《Journal of Crop Science and Biotechnology》2018,21(5):507-514
This study was conducted to investigate cytotoxic effect, phenolic content, DPPH, ABTS radical scavenging rate and nitrite scavenging rate from different solvent fractions of Codonopsis lanceolate root. At all extracts concentration, the cytotoxic effect on different fractions against human cancer cells was higher in n-hexane and butyl alcohol fractions than in the other fractions. The IC50 value on HeLa cell showed the lowest by 62.70 μg.mL-1 on n-hexane fraction, and exhibited the values of butyl alcohol fraction 341.36 μg.mL-1, methylene chloride 598.33 μg.mL-1, ethyl acetate fraction 860.44 μg.mL-1, DW fraction 2896.82 μg.mL-1. Total polyphenol content on different solvent fractions varied from 102.43 to 153.52 mg.g-1, and that of ethyl acetate fraction showed the highest amount. The DPPH and ABTS radical scavenging activity showed that the increase was proportional to the concentration, and the scavenging activity also showed the highest in ethyl acetate fraction. The nitrite scavenging activity of each fraction at pH 1.2 was in the order of EA > BA > MC > n-H > DW, and the lower the acidity, the higher nitrite scavenging activity, and there was no distinct detection of nitrite scavenging effects of the pH range 6.0. The results of this study suggested that the extract of Codonopsis lanceolate root may assist in the potential biological activities, and it was found that the activity was different depending on the organic solvent fraction and the water fraction. 相似文献