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121.
122.
Sang-Woo Lee Il-Pyung Ahn Sang-Yeon Sim Sue-Yeon Lee Myeon-Whoon Seo Soonok Kim Sook-Young Park Yong-Hwan Lee Seogchan Kang 《European journal of plant pathology / European Foundation for Plant Pathology》2010,126(1):1-11
A Gram-negative rhizobacterial isolate (LSW25) antagonistic to Pseudomonas corrugata, a vein necrosis pathogen of tomato, and promotes the growth of tomato seedlings was isolated from surface-sterilised tomato roots. A spontaneous rifampicin-resistant
mutant (LSW25R) was selected to facilitate its tracking, and identified as Pseudomonas sp. and named as Pseudomonas sp. LSW25R (LSW25R), based on its sequences of the internal transcribed spacer (ITS) region and 16 S rRNA gene. LSW25R inhibited
mycelial growth of 12 other plant fungal pathogens such as Botrytis cinerea on V8 agar plates. By using a scanning electron microscope, LSW25R colonised not only the root surface around the natural
aperture of tomato radicles but also under epidermal cells like endophytic bacteria. LSW25R successfully colonised the roots
of tomato, eggplant and pepper seedlings, significantly promoted the fresh weight, height and dry matter of tomato plants
at 108 cfu·ml−1, and increased the plant growth of eggplants and peppers at 104 cfu·ml−1, suggesting that the optimal population density of LSW25R for growth promotion varies from species to species. Moreover,
densities of LSW25R inside roots and the lowest leaf of tomato plants were > 9.3 × 103 cfu·g−1. Although the growth promotion of tomato by LSW25R was observed under N- or Ca-deficient conditions as well as a standard
nutrient condition, the uptake of calcium was increased only under the standard nutrient condition. In a hydroponic system,
LSW25R not only successfully colonised the rhizosphere during cultivation due to its broad spectrum of antifungal activity
and endophytic colonisation, but also reduced blossom-end rot of tomato fruits presumably through increasing calcium uptake. 相似文献
123.
In a previous study, we reported the grain weight QTL, tgw2 in the 150 F2:3 lines derived from a cross between Oryza sativa subssp. Japonica cv. Hwaseongbyeo and HG101. This QTL was confirmed in F4 lines (CR1242) segregating for the target region.
For fine mapping of tgw2, one F5 plant homozygous for the O. grandiglumis DNA in the target region was selected from CR1242 and crossed with Hwaseongbyeo to produce the F2 and F3 populations. QTL analysis using 490 F2 plants confirmed the existence of tgw2 with an R
2 value of 28.0%. This QTL explained 61.3% of the phenotypic variance for 1,000-grain weight in 64 F3 lines. Substitution mapping with 47 F3 lines and 74 F4 plants with informative recombination breakpoints in the target region was carried out to narrow down the position of the
tgw2. The result indicated that tgw2 was located in the 384-kb interval between two SSR markers, RM12813 and RM12836. Annotation data of BACs in this 384-kb region
revealed that forty-five putative genes exist in this interval including the GW2 gene responsible for grain weight and width. Considering the position of the QTL tgw2, it appears that tgw2 is functionally related to the gene GW2. However, the possibility that another unknown mechanism might be responsible for regulation of grain weight at tgw2 cannot be ruled out. Four QTLs for grain length, grain width, and grain thickness were also located in the same interval
suggesting that a single gene is involved in controlling these four traits. Substitution mapping also indicated that two QTLs
for grain weight and culm length, tgw2 and cl2, were tightly linked. 相似文献
124.
Ping-Rong Yuan Hyun-Jung Kim Qiong-Hua Chen Hong-Guang Ju Shi-Dong Ji Sang-Nag Ahn 《Journal of Crop Science and Biotechnology》2010,13(4):205-212
Quantitative trait loci (QTL) associated with four milling recovery properties, two chemical properties, six paste properties
of grain, and six textural parameters of cooked rice were identified using an introgression line (IL) population of rice developed
from an interspecific cross over two years. The IL population consisted of 121 lines from a cross between wild rice (O. rufipogon Griff.) and a japonica cultivar. A total of 28 QTLs were identified for the 14 traits using inter mapping. Of these, 10 were
significant over two years indicating that these QTLs are stable across years and environments. For eight (21%) of the QTLs
identified, the O. rufipogonderived alleles contributed a desirable effect on amylose content, protein content, minimum viscosity, final viscosity, and
consistency. Among these, pc3 for protein content and ac7 for amylose content were significant in both years and showed an R2 value of 25.5 and 30.9%, respectively. The markers closely associated with these useful alleles can be used to trace the
inheritance of specific chromosome segments in the IL population and also offer a starting point for map-based cloning of
genes underlying these traits. 相似文献
125.
OBJECTIVE: This paper examines trends in the neighbourhood food store environment (defined by the number and geographic density of food stores of each type in a neighbourhood), and in food consumption behaviour and overweight risk of 5779 men and women. DESIGN: The study used data gathered by the Stanford Heart Disease Prevention Program in four cross-sectional surveys conducted from 1981 to 1990. SETTING: Four mid-sized cities in agricultural regions of California. SUBJECTS: In total, 3154 women and 2625 men, aged 25-74 years. RESULTS: From 1981 to 1990, there were large increases in the number and density of neighbourhood stores selling sweets, pizza stores, small grocery stores and fast-food restaurants. During this period, the percentage of women and men who adopted healthy food behaviours increased but so did the percentage who adopted less healthy food behaviours. The percentage who were obese increased by 28% in women and 24% in men.ConclusionFindings point to increases in neighbourhood food stores that generally offer mostly unhealthy foods, and also to the importance of examining other food pattern changes that may have a substantial impact on obesity, such as large increases in portion sizes during the 1980s. 相似文献
126.
Joon Ha Park Tae-Kyeong Lee Dae Won Kim Ji Hyeon Ahn Choong-Hyun Lee Jong-Dai Kim Myoung Cheol Shin Jun Hwi Cho Jae-Chul Lee Moo-Ho Won Soo Young Choi 《Marine drugs》2022,20(4)
Astaxanthin is a powerful biological antioxidant and is naturally generated in a great variety of living organisms. Some studies have demonstrated the neuroprotective effects of ATX against ischemic brain injury in experimental animals. However, it is still unknown whether astaxanthin displays neuroprotective effects against severe ischemic brain injury induced by longer (severe) transient ischemia in the forebrain. The purpose of this study was to evaluate the neuroprotective effects of astaxanthin and its antioxidant activity in the hippocampus of gerbils subjected to 15-min transient forebrain ischemia, which led to the massive loss (death) of pyramidal cells located in hippocampal cornu Ammonis 1-3 (CA1-3) subfields. Astaxanthin (100 mg/kg) was administered once daily for three days before the induction of transient ischemia. Treatment with astaxanthin significantly attenuated the ischemia-induced loss of pyramidal cells in CA1-3. In addition, treatment with astaxanthin significantly reduced ischemia-induced oxidative DNA damage and lipid peroxidation in CA1-3 pyramidal cells. Moreover, the expression of the antioxidant enzymes superoxide dismutase (SOD1 and SOD2) in CA1-3 pyramidal cells were gradually and significantly reduced after ischemia. However, in astaxanthin-treated gerbils, the expression of SOD1 and SOD2 was significantly high compared to in-vehicle-treated gerbils before and after ischemia induction. Collectively, these findings indicate that pretreatment with astaxanthin could attenuate severe ischemic brain injury induced by 15-min transient forebrain ischemia, which may be closely associated with the decrease in oxidative stress due to astaxanthin pretreatment. 相似文献
127.
128.
We describe a case of gastrocnemius muscle rupture in a goral that got caught in a live trap set up to capture wild boars. We established a diagnostic method based on clinical signs and imaging examination findings, including magnetic resonance imaging for gastrocnemius muscle rupture with symptoms such as hindlimb lameness and plantigrade posture of unknown cause in wild animals. Although this condition is rare, this report will help veterinarians diagnose several diseases of the musculoskeletal and nervous systems in wild animals. This is the first report of successful diagnosis and treatment of gastrocnemius muscle rupture in gorals in Korea. 相似文献
129.
Ji-Su Ahn Ye Young Shin Su-Jeong Oh Min-Hye Song Min-Jung Kang So Yeong Park Phuong Thao Nguyen Dang Khoa Nguyen Hyoung Kyu Kim Jin Han Elena A. Vasileva Natalia P. Mishchenko Sergey A. Fedoreyev Valentin A. Stonik Yoojin Seo Byung-Chul Lee Hyung-Sik Kim 《Marine drugs》2022,20(11)
The diverse therapeutic feasibility of the sea urchin-derived naphthoquinone pigment, Echinochrome A (Ech A), has been studied. Simple and noninvasive administration routes should be explored, to obtain the feasibility. Although the therapeutic potential has been proven through several preclinical studies, the biosafety of orally administered Ech A and its direct influence on intestinal cells have not been evaluated. To estimate the bioavailability of Ech A as an oral administration drug, small intestinal and colonic epithelial organoids were developed from mice and humans. The morphology and cellular composition of intestinal organoids were evaluated after Ech A treatment. Ech A treatment significantly increased the expression of LGR5 (~2.38-fold change, p = 0.009) and MUC2 (~1.85-fold change, p = 0.08). Notably, in the presence of oxidative stress, Ech A attenuated oxidative stress up to 1.8-fold (p = 0.04), with a restored gene expression of LGR5 (~4.11-fold change, p = 0.0004), as well as an increased expression of Ly6a (~3.51-fold change, p = 0.005) and CLU (~2.5-fold change, p = 0.01), markers of revival stem cells. In conclusion, Ech A is harmless to intestinal tissues; rather, it promotes the maintenance and regeneration of the intestinal epithelium, suggesting possible beneficial effects on the intestine when used as an oral medication. 相似文献
130.
ABSTRACT Responses of rice to Magnaporthe grisea and Cochliobolus miyabeanus were compared. In Tetep, a rice cultivar resistant to both fungi, pathogen inoculation rapidly triggered the hypersensitive reaction (HR), resulting in microscopic cell death. In rice cv. Nakdong, susceptible to both pathogens, M. grisea did not cause HR, whereas C. miyabeanus caused rapid cell death similar to that associated with HR, which appeared similar to that observed in cv. Tetep, yet failed to block fungal ramification. Treatment with conidial germination fluid (CGF) from C. miyabeanus induced rapid cell death in both cultivars, suggesting the presence of phytotoxins in CGF. Pretreatment of cv. Nakdong with CGF significantly increased resistance to M. grisea, while the same treatment was ineffective against C. miyabeanus. Similarly, in cv. Nakdong, benzothiadiazole (BTH) significantly increased resistance to M. grisea, but was ineffective against C. miyabeanus. Methyl jasmonate (MeJA) treatment appeared to be ineffective against either fungus. Increased resistance of cv. Nakdong to M. grisea by BTH or CCF treatment was correlated with more rapid induction of three monitored PR genes. Application of MeJA resulted in the expression of JAmyb in cv. Nakdong being induced faster than in untreated plants in response to M. grisea infection. In contrast, the expression pattern of the PR and JAmyb genes in response to C. miyabeanus was nearly identical between cvs. Nakdong and Tetep, and neither BTH nor MeJA treatment significantly modified their expression patterns in response to C. miyabeanus infection. Our results suggest that rice employs distinct mechanisms for its defense against M. grisea versus C. miyabeanus. 相似文献