Enhanced non-extractable residue formation of 14C-metalaxyl catalyzed by an immobilized laccase

We studied the influence of an immobilized laccase from Trametes versicolor on non-extractable residue (NER) formation of the systemic fungicide ¹⁴C-metalaxyl in soil. We added the enzyme (130 mU/g DW) to soil sterilized by gamma irradiation and observed that the amount of NER (6.3 % of applied radioactivity) after 10 days of incubation was enhanced about twofold compared to the sterile soil without laccase addition. Residues formed within samples without enhanced enzyme activity were mainly bound via ester linkages to all fractions of humic matter, i.e., fulvic acids, humic acids, non-humines, and humines, respectively. In contrast, residues formed in presence of immobilized laccase were more strongly bound by covalent linkages such as ether and C-C bonds, especially to humic acids. After chemical degradation of the humic matter, it could be observed that all NER contained the first major transformation product, i.e., metalaxyl acid. The findings underline that the residue formation of metalaxyl in soil may be partly catalyzed by immobilized extracellular oxidative enzymes through oxidative coupling reactions within the humic matter.     

Plant available soil water at sowing in Mediterranean environments—Is it a useful criterion to aid nitrogen fertiliser and sowing decisions?

In regions where rainfall is low and variable, water stored in the soil profile prior to sowing can alter yield expectation and hence management decisions. Thus, wheat farmers in Mediterranean regions may be able to benefit from knowing the amount of soil water at sowing by optimising their nitrogen (N) fertiliser management and by deciding on whether or not to sow a crop. We used the ASPIM-Nwheat model to explore how levels of plant available soil water (PAW) at sowing, N fertiliser rate, soil, site and season-type (below or above median rainfall) affected wheat yields at sites in the Mediterranean area of southwest Australia. Overall, the greatest influence on yield potential and the consequent N fertilisation requirement was season-type. The additional yield per mm PAW at sowing was generally higher in seasons with below median rainfall, except when yields were severely water-limited by below median rainfall of <222 mm combined with <40 mm PAW at sowing on light clay soil with 109 mm plant available water capacity (PAWC). Sowing was generally warranted; only on light clay soil with <10 mm PAW at sowing and below median rainfall of <222 mm was there an opportunity for a conditional sowing strategy. Scope for varying N fertiliser rates with PAW at sowing was limited to soils with higher PAWC (109 and 130 mm, respectively) in below median rainfall seasons at the wetter site (295 mm mean seasonal rainfall), and in both season-types at the drier site (225 mm mean seasonal rainfall). Only in these combinations, soil water at sowing modified the optimal N fertiliser rate for maximum average yield resulting in significant interactions between PAW at sowing and N fertiliser rates. Similar interactions were found for a site in the Mediterranean Basin and a site in the eastern Australian subtropics on soil with high PAWC (183 and 276 mm, respectively). In contrast, there was no benefit from modifying crop management based on PAW at sowing on soil with low PAWC (i.e. sandy soil) and/or under conditions of high in-season rainfall. The conditional N management approach becomes more viable as the proportion of water stored in the soil prior to sowing increases relative to total crop water use and as the PAWC of the soil increases. Knowledge of PAW at sowing × N fertiliser rate interactions in a particular soil × site × season-type context can help to identify sites where a more targeted N management dependent on amounts of PAW at sowing is potentially profitable.     

甘蔗糖料压榨系统的现况及新技术方案

本文论述了辊式压榨机的现状及其技术改进趋势,并分析了其固有缺陷,认为这是造成甘蔗汁液排放困难、重吸现象严重的根本原因,进而提出了改变压榨形式,延长压榨时间,采用外力排放糖汁等新的压榨技术方案,通过改善甘蔗糖料的压榨过程,强化固液萃取(渗出)分离过程,消除重吸现象,提高压榨效率,取代传统辊式压榨机,降低能耗,以满足清洁生产之需要.     

Agricultural intensification in the Sahel – The ladder approach

Agricultural intensification in the Sahel can be described as climbing a ladder. The capital, labour, management and institutional requirements increase when farmers climb the ladder, but the potential gains are also higher. The first step on this ladder are agricultural practices without any financial outlay but with increasing labour demand, such as organic fertilizer use, seed priming, water harvesting and harvesting grains at physiological maturity to improve fodder quality. The next step on the ladder is the use of micro-fertilising, popularly known as microdose, at the rate of 0.3 g NPK fertilizer per pocket in sorghum and millet. The following step is the development of improved crop/livestock systems characterized by use of higher rates of mineral fertilisers and manure, increasing cowpea density and improved animal fattening. The last step presented on the ladder is the development of more commercially orientated agriculture characterized by development of cash crops, milk production and/or agroforestry systems. Evidences from the field support the observation that farmers intensify their production in a sequential manner similar to the way described in this paper. The technologies presented can facilitate agricultural intensification by reducing the risks and minimising the cost in agricultural production.     

Plagiotropism and auxin in Abies nordmanniana

Main branches of Abies nordmanniana Spach. were examined through their first growth season from subapical buds around the leader bud to fully expanded shoots. Plagiotropism was evident in branch orientation, which was almost horizontal, as well as in the orientation of buds developing on the branches. Auxin transport capacity was predominantly basipetal (> 90%) and consistently higher in the middle part of the branch than in the distal and proximal ends. Auxin transport capacity was higher on the dorsal side of the branch during the short initial hyponastic growth phase, but the difference disappeared when the branch became horizontal. No dorsal-ventral differences could be detected in young horizontal branches in concentrations of indole acetic acid, cytokinins, gibberellins or abscisic acid. Branch orientation was unaffected by decapitation of the leader apex or by decapitation and replacement with exogenous auxin. However, decapitation resulted in a less plagiotropic bud arrangement on the branches, and auxin application to the leader bud scar counteracted this effect. Thus, a signal originating in the stem seems to be involved in regulating branch bud positioning, whereas the horizontal branch orientation must rely on a different mechanism, presumably autonomic within the branch.     

土壤中山毛榉根系呼吸的碳素损失

根系对植物生长发育过程有着重要的调控作用,其生理生化代谢过程是农学、生态学等学科研究的薄弱领域。植物生长发育过程受不同器官呼吸碳素损失的影响,根系是一个重要的碳素库［1］,其呼吸作用是生态系统碳素循环中重要的反馈途径之一［2,3］。由于很多研究侧重地上部分的研究,就环境因素对地下碳素库调控的了解还很有限,根系碳素量几乎占到植物总碳素量的50%［4,5］,但是,目前就植物根系呼吸碳素损失的研究方法,以及影响同化产物累积和分配的机制还有待研究［1］。以往多用溶液培养、气培和分离根系［6］来测定根系的呼吸速率或根系活力,这些测定结果与自然状态下根系的实际生理状况差异较大,难以确切掌握植物碳素平衡与分配。为此,我们设计了一种可动态测定土壤中植物根系呼吸碳素损失的方法,并在高CO2条件下植物13C吸收与分配模式研究中取得了较好的结果。     

双螺杆挤压机螺杆元件类型对扭矩和压力的影响

以食用脱脂低温豆粕为原料,以Brabender DSE-25型双螺杆挤压膨化实验室工作站为加工设备,研究了不同几何参数的输送元件、捏合块和齿型盘6种螺杆元件对挤压过程中扭矩和压力的影响。结果表明:螺杆元件对扭矩和压力有显著影响;对扭矩影响的主次顺序为反向输送元件;齿型盘、捏合块、正向输送元件;有反向螺旋角或错裂角或对物料剪切强度大的元件能够显著增加扭矩;元件对压力的影响规律不明显。对系统稳定性的初步分析认为,错列角为45°和90°的捏合块具有较好的混炼效果,而齿型盘和错列角为-45°的捏合块的混炼效果较差。     

Predacious activity of the nematode-trapping fungus Duddingtonia flagrans against cyathostome larvae in faeces after passage through the gastrointestinal tract of horses

This study was undertaken to examine the potential of the nematode-trapping microfungus Duddingtonia flagrans to survive passage through the gastrointestinal tract of horses and subsequently to destroy free-living stages of cyathostomes in faecal cultures. Three different oral dose levels were tested, two horses being used for each level. Faeces were collected twice daily and the number of parasite eggs per gram of faeces were determined. The numbers of infective third stage larvae which developed in faecal cultures were determined after the cultures had been incubated for 2 weeks at 24°C. Results showed a positive relationship between dose level and reduction in the number of infective larvae. Fungi were recovered in faeces at times which corresponded to high larval reduction.