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91.
Uptake and leakage experiments were performed to study the effects of copper and cadmium on K(+) fluxes in birch (Betula pendula Roth) roots. Labeled rubidium ((86)Rb(+)) was used as a tracer for K(+). Plants were pretreated with Cu or Cd (0, 2, 5 or 25 microM) for 0-300 min and then transferred to radiolabeled nutrient solution (150 microM K(+)) with or without 2,4-dinitrophenol (DNP) to separate the effects of heavy metal on active and passive K(+) influxes. Passive K(+) influx was decreased by pretreatment with Cu but was only slightly affected by pretreatment with Cd. Pretreatment with 2 microM Cu increased active K(+) influx, whereas pretreatment with 25 microM Cu decreased active K(+) influx and intermediate Cu concentrations (5 microM) did not affect active K(+) influx. The pretreatment effects of Cu on active and passive K(+) influxes increased with increasing pretreatment time. During the first hour, pretreatment with Cd decreased active K(+) influx with increasing pretreatment time, whereafter recovery began. To measure K(+) efflux, birch plants were loaded with (86)Rb(+) for 7 days before being exposed to Cu or Cd (0, 1, 3, 5 or 10 microM) in unlabeled nutrient solutions for 24 h. Net efflux of K(+) was measured as (86)Rb(+)-activity in the nutrient solutions 24 h after a heavy metal had been introduced. Efflux of K(+) increased with increasing Cu or Cd concentration in the unlabeled nutrient solution.The data indicate that Cu and Cd affected K(+) influx differently and that recovery mechanism(s), which were induced shortly after heavy metal introduction, counteracted the heavy-metal induced inhibition of active K(+) influx. Efflux of K(+) from plant roots over a 24-h period indicated that Cu and Cd had similar effects on K(+) efflux. There was no evidence of a recovery mechanism counteracting the heavy-metal-induced inhibition of K(+) efflux. 相似文献
92.
93.
Cory W. Whitney Eike Luedeling John R. S. Tabuti Antonia Nyamukuru Oliver Hensel Jens Gebauer Katja Kehlenbeck 《Agriculture and Human Values》2018,35(2):399-424
Homegardens are traditional food systems that have been adapted over generations to fit local cultural and ecological conditions. They provide a year-round diversity of nutritious foods for smallholder farming communities in many regions of the tropics and subtropics. In southwestern Uganda, homegardens are the primary source of food, providing a diverse diet for rural marginalized poor. However, national agricultural development plans as well as economic and social pressures threaten the functioning of these homegardens. The implications of these threats are difficult to evaluate, because the structure and functions of the homegardens are not well understood. The aim of the study was to identify patterns and influencing factors in the diversity of homegardens by documenting the floristic diversity and its interactions with spatial, environmental and socio-economic factors. A geographically and socially focused assessment of floristic diversity in 102 randomly selected homegardens in three districts of southwest Uganda was conducted along a deforestation gradient following a human ecology conceptual framework and testing multiple quantitative hypotheses regarding the above mentioned factors. A merged mixed-method approach was followed to provide context and feedback regarding quantitative findings. Results show a high total richness of 209 (mean 26.8 per homegarden) crop species (excluding weeds and ornamentals) dominated by food species, which constituted 96 percent of individuals and 44 percent of all species. Forest-edge homegardens maintained higher plant diversity compared to homegardens in deforested areas and near degraded wetlands. Multiple linear regression models indicated elevation, location, homegarden size, distance to market, additional land ownership (outside the homegarden) and livestock ownership as significant predictors of crop diversity. Cluster analysis of species densities revealed four garden types: ‘diverse tree gardens’, ‘small forest-edge gardens’, ‘large, old, species-rich gardens’, and ‘large, annual-dominated herb gardens’, with 98% correct classification. Location, elevation, and garden size were also important determinants in the cluster assignment. We conclude that the diversity of the studied homegardens may be changing as part of adaptive traditional practices and in response to external drivers. The identified patterns illustrate the importance of homegardens for rural livelihoods and may offer some ways to support farmers to maintain these systems as relevant mechanisms for development in Uganda. 相似文献
94.
95.
Ken Steen Pedersen Markku Johansen ?ystein Angen Sven Erik Jorsal Jens Peter Nielsen Tim K Jensen Roberto Guedes Marie St?hl Poul B?kbo 《Irish veterinary journal》2014,67(1)
Background
The major indication for antibiotic use in Danish pigs is treatment of intestinal diseases post weaning. Clinical decisions on antibiotic batch medication are often based on inspection of diarrhoeic pools on the pen floor. In some of these treated diarrhoea outbreaks, intestinal pathogens can only be demonstrated in a small number of pigs within the treated group (low pathogen diarrhoea). Termination of antibiotic batch medication in herds suffering from such diarrhoea could potentially reduce the consumption of antibiotics in the pig industry. The objective of the present pilot study was to suggest criteria for herd diagnosis of low pathogen diarrhoea in growing pigs.Data previously collected from 20 Danish herds were used to create a case series of clinical diarrhoea outbreaks normally subjected to antibiotic treatment. In the present study, these diarrhoea outbreaks were classified as low pathogen (<15% of the pigs having bacterial intestinal disease) (n =5 outbreaks) or high pathogen (≥15% of the pigs having bacterial intestinal disease) (n =15 outbreaks). Based on the case series, different diagnostic procedures were explored, and criteria for herd diagnosis of low pathogen diarrhoea were suggested. The effect of sampling variation was explored by simulation.Results
The diagnostic procedure with the highest combined herd-level sensitivity and specificity was qPCR testing of a pooled sample containing 20 randomly selected faecal samples. The criteria for a positive test result (high pathogen diarrhoea outbreak) were an average of 1.5 diarrhoeic faecal pools on the floor of each pen in the room under investigation and a pathogenic bacterial load ≥35,000 per gram in the faecal pool tested by qPCR. The bacterial load was the sum of Lawsonia intracellularis, Brachyspira pilosicoli and Escherichia coli F4 and F18 bacteria per gram faeces. The herd-diagnostic performance was (herd-level) diagnostic sensitivity =0.99, diagnostic specificity =0.80, positive predictive value =0.94 and negative predictive value =0.96.Conclusions
The pilot study suggests criteria for herd diagnosis of low pathogen diarrhoea in growing pigs. The suggested criteria should now be evaluated, and the effect of terminating antibiotic batch medication in herds identified as suffering from low pathogen diarrhoea should be explored.Electronic supplementary material
The online version of this article (doi:10.1186/2046-0481-67-24) contains supplementary material, which is available to authorized users. 相似文献96.
A risk-factor study was performed in eight dairy herds found to excrete verocytotoxin-producing Escherichia coli (VTEC) O157 in a former prevalence study. Associations between excretion of VTEC O157 and management factors such as housing and feeding were analysed in a generalised linear mixed model. The animals were stratified in three age groups and sampled four times during 1 year. The risk of excreting VTEC O157 was higher among weaned calves than non-weaned calves. Among the calves aged 1–4 months, the risk was reduced if the calf had suckled colostrum from the mother or if the calf had stayed >2 days with the mother after calving. Calves aged 5–24 months that had been moved within the last 2 weeks had a higher risk, but risk was reduced if fed barley silage. Cows fed grain or molasses had a higher risk of excreting VTEC O157. 相似文献
97.
Romain Gastineau Fran?ois Turcotte Jean-Bernard Pouvreau Michèle Moran?ais Jo?l Fleurence Eko Windarto Fiddy Semba Prasetiya Sulastri Arsad Pascal Jaouen Mathieu Babin Laurence Coiffard Céline Couteau Jean-Fran?ois Bardeau Boris Jacquette Vincent Leignel Yann Hardivillier Isabelle Marcotte Nathalie Bourgougnon Réjean Tremblay Jean-Sébastien Deschênes Hope Badawy Pamela Pasetto Nikolai Davidovich Gert Hansen Jens Dittmer Jean-Luc Mouget 《Marine drugs》2014,12(6):3161-3189
In diatoms, the main photosynthetic pigments are chlorophylls a and c, fucoxanthin, diadinoxanthin and diatoxanthin. The marine pennate diatom Haslea ostrearia has long been known for producing, in addition to these generic pigments, a water-soluble blue pigment, marennine. This pigment, responsible for the greening of oysters in western France, presents different biological activities: allelopathic, antioxidant, antibacterial, antiviral, and growth-inhibiting. A method to extract and purify marennine has been developed, but its chemical structure could hitherto not be resolved. For decades, H. ostrearia was the only organism known to produce marennine, and can be found worldwide. Our knowledge about H. ostrearia-like diatom biodiversity has recently been extended with the discovery of several new species of blue diatoms, the recently described H. karadagensis, H. silbo sp. inedit. and H. provincialis sp. inedit. These blue diatoms produce different marennine-like pigments, which belong to the same chemical family and present similar biological activities. Aside from being a potential source of natural blue pigments, H. ostrearia-like diatoms thus present a commercial potential for aquaculture, cosmetics, food and health industries. 相似文献
98.
Renate Kvingedal Brad S. Evans Joseph J.U. Taylor Jens Knauer Dean R. Jerry 《Aquaculture (Amsterdam, Netherlands)》2008,279(1-4):23-28
To understand the influence the environment and associated genotype by environment interactions will exert on future silver-lip pearl oyster (Pinctada maxima) selective breeding programs, this study assessed the relative performance in four shell growth traits of spat from five full-sib families, when spat were communally reared at different salinities (29, 34 and 40 ppt), food availability (high, medium and low), food quality (high, medium and low), and in a hatchery vs. ocean environment for 43 days.Rearing environment was found to influence growth expression, with significant differences evident when oysters were grown at different salinities in the ocean instead of hatchery, or when fed algae of differing nutritional quality. As indicated by MANOVA, family comparative growth performances were also altered when the environment changed, with significant environment by family interactions apparent in the food quality, food availability and hatchery vs. ocean rearing treatments. Changes in salinity, however, did not affect relevant family performances.These results indicate that growth and relative family performance in P. maxima may change dependent on local environmental conditions and that genotype by environment effects may need to be considered in breeding programs for this species. 相似文献
99.
Paul Chittaro Jens Hegg Abby Fuhrman Devin Robichaux Rothboury Doung Brian Beckman Chris Fisher Jeff Vervoort Brian Kennedy 《Ecology of Freshwater Fish》2023,32(1):107-119
Effective species management often requires understanding patterns of movement and habitat use. A common approach in identifying where individuals reside relies upon chemical tracers from the environment that are incorporated into an individual's tissues. For fish, isotopes in their otoliths, specifically the portion of their otolith formed during their larval stage, have been used to identify the natal origin. Complicating this work, however, is the fact that during this life stage, there is a shift in the source of isotopes deposited onto the growing otolith from maternally to environmentally derived. The objective of this study was to identify the portion of the otolith representing this transition to environmentally derived isotopes so as to accurately investigate questions of natal origin for a threatened population of fall Chinook salmon (Oncorhynchus tshawytscha). We exposed developing larvae to four treatments that differed in terms of their water strontium isotope ratio (87Sr/86Sr) and used change-point analysis of otolith 87Sr/86Sr and strontium to calcium ratio (Sr/Ca) to identify the otolith radius corresponding to the transition to environmentally derived isotopes. Our results indicated this transition occurred, on average, at 132 μm (87Sr/86Sr; ±50 μm standard deviation) and 127 μm (Sr/Ca; ±29 μm) from the otolith core, which corresponded to the developmental time between hatching and exogenous feeding. A substantial proportion of our otoliths (i.e., 61%) did not show convergence between otolith and water 87Sr/86Sr by the end of the 113-day experiment, which was likely due to the dietary contribution of marine-based feed. Therefore, we were unable to recommend an otolith radius to target for the purposes of reconstructing natal origin apart from being beyond approximately 130 μm. 相似文献
100.