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41.
Two forms of vitellogenin were isolated by DEAE agarose ion-exchange chromatography from plasma of the tilapia, Oreochromis mossambicus. The monomers have apparent molecular masses of 200 and 130 kDa, as indicated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE),
and a total amount of phosphorus of 1.7 and 0.1%, respectively. Antibodies specific to the two forms, designated tVTG-200
and tVTG-130, were generated in rabbits and used to develop enzyme-linked immunosorbent assays (ELISAs) and in Western blot
analyses of plasma and oocyte extract. SDS-PAGE of the oocyte extract showed a major protein band at 106.6, minor bands at
26.6, 24.2, and 23.7 kDa, and very faint bands at 83.4 and 17.5 kDa. Western blots of the oocyte extract revealed that the
antiserum to tVTG-200 recognized strongly the protein bands at 24.2 and 23.7 kDa, and less strongly the bands at 25.1 and
22.6 kDa, whereas the antiserum to tVTG-130 recognized mainly the protein band at 106.6 kDa. The presence of both VTGs in
untreated male tilapia was detected with the ELISAs using relatively high plasma volumes. Their presence in males was confirmed
by VTG-like immunoreactive materials eluting from the ion-exchange column at the same positions as tVTG-200 and tVTG-130.
The concentrations of the VTGs in males were several orders of magnitude lower than in vitellogenic females. Treatment of
male tilapia with estradiol-17β (E2) induced both VTGs within 24h. After 7 days, tVTG-130 reached a maximum concentration in plasma, whereas tVTG-200 continued
to increase. Our findings demonstrate that the two vitellogenins are biochemically distinct, possibly differentially regulated,
and made by both sexes. 相似文献
42.
Anne H. Beaudreau Eric J. Ward Richard E. Brenner Andrew O. Shelton Jordan T. Watson Jennifer C. Womack Sean C. Anderson Alan C. Haynie Kristin N. Marshall Benjamin C. Williams 《Fish and Fisheries》2019,20(4):601-619
Heterogeneity in human responses and decision‐making can contribute to the resilience of social–ecological systems in the face of environmental, political and economic pressures. In fishery systems worldwide, the ability of harvesters to maintain a diverse portfolio of fishing strategies is important for building adaptive capacity. We used a case‐study approach to examine the complexity of factors that inhibit or promote diversification in fisheries of Alaska, one of the major fishing regions of the world. Through a combination of harvest records and literature review, we explored shifts in participation and portfolio diversity in Alaskan fisheries over three decades. The four case‐studies examined the responses of fishers, fleets and communities to multiple, intersecting pressures, including biological declines, market and price dynamics, fishery privatization and the 1989 Exxon Valdez oil spill. These cases illustrate how stressors acting at multiple scales can encourage or constrain opportunities for diversification, and that these opportunities may be spread inequitably across participants. Overall, we found evidence for reduced participation and increasing specialization in Alaskan commercial fisheries. While numerous factors explain these trends, policies like individual quota systems and the increasing cost of entry into fisheries are forcing consolidation at local to regional scales. A portfolio approach to managing fisheries that reduces barriers to diversification and includes broad representation of resource users and communities in management may help to maintain opportunity and choice for fishers. 相似文献
43.
Brown shrimp (Farfantepenaeus aztecus) density distribution in the Northern Gulf of Mexico: an approach using boosted regression trees
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Jose T. Montero Tanya A. Chesney Jennifer R. Bauer John T. Froeschke Jim Graham 《Fisheries Oceanography》2016,25(3):337-348
The estuarine‐dependent brown shrimp, Farfantepenaeus aztecus, is a significant commercial fishery and important species in the Gulf of Mexico (GOM) ecosystem as well as being a key component in energy transfer between benthic and pelagic food web systems. Because of the economical and ecological importance of brown shrimp, we developed a spatial population model to identify places of high shrimp density under a set of spatial, environmental and temporal variables in the Northern Gulf of Mexico (NGOM). We used fisheries‐independent data collected by the Southeast Area Monitoring and Assessment Program (SEAMAP) from 1992 to 2007 (summer and fall seasons). The relationship between the predictor variables and shrimp density was modeled using Boosted Regression Trees (BRT). Within the environmental variables included in the model, bottom type and depth of the water column were the most important predictors of shrimp density in the NGOM. Spatial predictions performed using the trained BRT model for summer and fall seasons showed a spatial segregation of shrimp density. During the summer, higher densities were predicted near the Texas and Louisiana coast and during the fall, higher densities were predicted further offshore. The model performed well and allowed successful prediction of brown shrimp hot spots in the NGOM. Model results allow fisheries managers to evaluate the potential impact from fisheries on the resource and to develop future fisheries management strategies, understand the biology of brown shrimp as well as assess the potential impacts of oil spills or climate change. 相似文献
44.
Bahiagrass (Paspalum notatum) is a warm-season grass used primarily in pastures and along highways and other low maintenance public areas in Florida.
It is also used in landscapes to some extent because of its drought tolerance. Bahiagrass can survive under a range of moisture
conditions from no irrigation to very wet conditions. Its well-watered consumptive use has not been reported previously. In
this study, bahiagrass crop coefficients (K
c) for an irrigated pasture were determined for July 2003 through December 2006 in central Florida. The eddy correlation method
was used to estimate crop evapotranspiration (ETc) rates. The standardized reference evapotranspiration (ETo) equation (ASCE-EWRI standardization of reference evapotranspiration task committee report, 2005) was applied to calculate
ETo values using on site weather data. Daily K
c values were estimated from the ratio of the measured ETc and the calculated ETo. The recommended K
c values for bahiagrass are 0.35 for January–February, 0.55 for March, 0.80 for April, 0.90 for May, 0.75 for June, 0.70 for
July–August, 0.75 for September, 0.70 for October, 0.60 for November, and 0.45 for December in central Florida. The highest
K
c value of 0.9 in May corresponded with maximum vapor pressure deficit conditions as well as cloud free conditions and the
highest incoming solar radiation as compared to the rest of the year. During the summer (June to August), frequent precipitation
events increased the cloud cover and reduced grass water use. The K
c annual trend was similar to estimated K
c values from another well-watered warm-season grass study in Florida. 相似文献
45.
Earthworms are recognized to play an important role in the decomposition of organic materials. To test the use of earthworms as an indicator of plant litter decomposition, we examined the abundance and biomass of earthworms in relation to plant litter decomposition in a tropical wet forest of Puerto Rico. We collected earthworms at 0–0.1 m and 0.1–0.25 m soil depths from upland and riparian sites that represent the natural variation in soils and decomposition rates within the forest. Earthworms were hand-sorted and weighed for both fresh and dry biomass. Earthworms were dominated by the exotic endogeic species Pontoscolex corethrurus Müller; they were more abundant, and had higher biomasses in the upland than in riparian sites of the forest. Plant leaf litter decomposed faster in the upland than riparian sites. We found that earthworm abundance in the upper 0.1 m of the soil profile positively correlated with decomposition rate of plant leaf litter. Ground litter removal had no effect on the abundance or biomass of endogeic earthworms. Our data suggest that earthworms can be used to predict decomposition rates of plant litter in the tropical wet forest, and that the decomposition of aboveground plant litter has little influence on the abundance and biomass of endogeic earthworms. 相似文献
46.
Using soil temperature and moisture to predict forest soil nitrogen mineralization 总被引:10,自引:0,他引:10
Due to the importance of N in forest productivity ecosystem and nutrient cycling research often includes measurement of soil N transformation rates as indices of potential availability and ecosystem losses of N. We examined the feasibility of using soil temperature and moisture content to predict soil N mineralization rates (Nmin) at the Coweeta Hydrologic Laboratory in the southern Appalachians. We conducted seasonal laboratory incubations of A and AB horizon soils from three sites with mixed-oak vegetation using temperature and moisture levels characteristic of the season in which the soils were collected. The incubations showed that temperature and temperature-moisture interactions significantly affected net soil Nmin. We used the laboratory data to generate equations relating net Nmin to soil temperature and moisture data. Using field-collected temperature and moisture data we then calculated Nmin on similar forest sites and compared predicted rates with in situ, closed-core Nmin measurements. The comparison showed that the in situ Nmin was greater than rates predicted from laboratory generated equations (slope =3.22; r2=0.89). Our study suggests that while climatic factors have a significant effect on soil Nmin, other factors also influence rates measured in the laboratory and in situ. 相似文献
47.
Solan M Cardinale BJ Downing AL Engelhardt KA Ruesink JL Srivastava DS 《Science (New York, N.Y.)》2004,306(5699):1177-1180
Rapid changes in biodiversity are occurring globally, yet the ecological impacts of diversity loss are poorly understood. Here we use data from marine invertebrate communities to parameterize models that predict how extinctions will affect sediment bioturbation, a process vital to the persistence of aquatic communities. We show that species extinction is generally expected to reduce bioturbation, but the magnitude of reduction depends on how the functional traits of individual species covary with their risk of extinction. As a result, the particular cause of extinction and the order in which species are lost ultimately govern the ecosystem-level consequences of biodiversity loss. 相似文献
48.
49.
Adams Alison B. Pontius Jennifer Galford Gillian Gudex-Cross David 《Landscape Ecology》2019,34(10):2401-2419
Landscape Ecology - Understanding how the Northern Forest landscape has changed and is likely to change, both in terms of forest extent and forest configuration, has important implications for... 相似文献
50.
Snyder Marcía N. Schumaker Nathan H. Ebersole Joseph L. Dunham Jason B. Comeleo Randy L. Keefer Matthew L. Leinenbach Peter Brookes Allen Cope Ben Wu Jennifer Palmer John Keenan Druscilla 《Landscape Ecology》2019,34(5):1197-1197
Landscape Ecology - In the original publication of the article, the third author name has been misspelt. The correct name is given in this Correction. The original version of this article was revised. 相似文献