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Effect of different penetrating and non‐penetrating cryoprotectants and media temperature on the cryosurvival of vitrified in vitro produced porcine blastocysts 下载免费PDF全文
Louise Katherine Bartolac Jenna Louise Lowe George Koustas Christopher Gerald Grupen Cecilia Sjöblom 《Animal Science Journal》2018,89(9):1230-1239
The aim of this study was to determine the most efficient vitrification protocol for the cryopreservation of day 7 in vitro produced (IVP) porcine blastocysts. The post‐warm survival rate of blastocysts vitrified in control (17% dimethyl sulfoxide + 17% ethylene glycol [EG] + 0.4 mol/L sucrose) and commercial media did not differ, nor did the post‐warm survival rate of blastocysts vitrified in medium containing 1,2‐propandiol in place of EG. However, vitrifying embryos in EG alone decreased the cryosurvival rate (55.6% and 33.6%, respectively, p < .05). Furthermore, the post‐warm survival rates of blastocysts vitrified with either trehalose or sucrose as the non‐penetrating cryoprotectant did not differ. There was also no significant difference in post‐warm survival of blastocysts vitrified in control (38°C) media and room temperature (22°C) media with extended equilibration times, although when blastocysts were vitrified using control media at room temperature, the post‐warm survival rate increased (56.8%, 57.3%, 72.5%, respectively, p < .05). The findings show that most cryoprotectant combinations examined proved equally effective at supporting the post‐warm survival of IVP porcine blastocysts. The improved post‐warm survival rate of blastocysts vitrified using media held at room temperature suggests that the cryoprotectant toxicity exerted in 22°C media was reduced. 相似文献
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Jenna M. Malone John G. Virtue Chris Williams Christopher Preston 《Weed Biology and Management》2017,17(1):17-28
The perennial grass, Arundo donax, has shown potential as a promising biomass crop. However, it has become invasive in a number of areas and declared a noxious weed in some jurisdictions, making proposals to grow A. donax for commercial use in Australia controversial. Evidence of asexual reproduction and the presence of a single genetic clone in Australia was investigated, as such characteristics would indicate a limited risk of escape and invasion. Using amplified fragment length polymorphism markers, the genetic diversity of 218 A. donax samples from across Australia was examined. The samples were found to separate into two distinct genetic groups, or clades. There was only a small amount of genetic diversity within a clade (0.9 and 1.5%). However, there was a larger difference between the clades of 19.8%, suggesting the presence of two distinct A. donax genotypes in Australia. The low level of genetic variation in Australian A. donax that was found in this study indicates that spread is essentially by vegetative means and suggests that if grown in areas where it is separated from natural water dispersal events, A. donax poses a low risk of becoming invasive. 相似文献
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Alicia B Merriam Jenna M Malone James P Hereward Gurjeet Gill Christopher Preston 《Pest management science》2023,79(9):3200-3210
Background
Annual sowthistle is a weed that is difficult to control in lentil crops in southern Australia due to a lack of herbicide options, widespread herbicide resistance and prolific production of highly mobile seed. This study investigates herbicide resistance in annual sowthistle in the Mid-North (MN) and Yorke Peninsula (YP) regions of South Australia, identifies and characterizes the mechanisms of acetolactate-synthase (ALS)-inhibitor resistance in this amphidiploid species, and combines this with analyses of population structure and gene flow.Results
ALS-inhibitor-resistant annual sowthistle is widespread across the YP and MN of South Australia and is associated with a variety of Proline-197 mutations of the ALS gene, including leucine, alanine, arginine, serine, threonine and histidine. These mutations were found in different combinations on either of the two copies of the ALS gene. An additional 200 tissue samples were collected from across a single field on the YP and the ALS gene was sequenced for all these individuals. Different ALS-inhibitor resistance profiles were evident between mutation combinations and within mutation combinations, possibly mediated by differing subgenome assortment of the mutations, or altered gene experession of the two ALS homeologs. Population genetics analysis showed evidence of long-distance dispersal, resulting in highly mobile resistance genes, and multiple instances of resistance mutation evolution.Conclusions
Continuing selection of Sonchus oleraceus populations with ALS-inhibiting herbicides has resulted in the accumulation of additional mutations within the ALS gene. New practices to control herbicide-resistant S. oleraceus should be examined, and control should focus on reducing seed set and dispersal to prevent the spread of emerging cases of resistance. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. 相似文献28.
Nicole S. Yang Eric G. Johnson Carrie A. Palm Jenna H. Burton Robert B. Rebhun Michael S. Kent William T.N. Culp 《Veterinary radiology & ultrasound》2023,64(1):105-112
Magnetic resonance imaging (MRI) has been used to evaluate dogs with suspected prostatic neoplasia, however, published studies describing MRI characteristics of canine prostatic neoplasia are currently lacking. The aims of the current retrospective case series study were to describe MRI findings of the pelvic region in dogs with a histopathologic or cytologic diagnosis of prostatic neoplasia. Retrospective analysis of these images was then performed by a board-certified veterinary radiologist for shared imaging characteristics. The most consistent characteristics were heterogeneous hyperintensity of the tumor on T2-weighted images (10/10) and short tau inversion recovery images (10/10), prostatic capsular margin distortion by the tumor (10/10), cavitations (10/10), complete effacement of the prostatic architecture (9/10), neurovascular bundle (NVB) compression or invasion (9/10), heterogeneous isointensity of the tumor on T1-weighted images (9/10), and strong contrast enhancement of the tumor (8/10). Additional features included an overlying pattern of distorted radiating striations (7/10), regional lymphadenomegaly (5/10), mineralization within the mass (5/10), urinary bladder trigone involvement (6/10), and post-prostatic urethral involvement (7/10). These findings supported the use of MRI as an adjunct imaging modality for diagnosis and therapeutic planning of prostatic neoplasia and including prostatic neoplasia as a likely differential diagnosis for dogs with these MRI characteristics. 相似文献
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Lucie Noall Suhwon Lee Jenna H. Burton Taya M. Marquardt Justin Cermak Lori A. Thombs Anita M. Rogic Jeffrey N. Bryan Shirley Chu 《Veterinary and comparative oncology》2023,21(3):509-519
Feline oral squamous cell carcinoma (FOSCC) is an aggressive cancer in domestic cats that has no effective treatment option when advanced. Preventative or early diagnostic measures are thus crucial. FOSCC is also a model for human head and neck SCC (HNSCC); strong risk factors in HNSCC include exposure to alcohol, tobacco, areca nut, and high-risk human papillomavirus. Previous studies have identified flea collar and tobacco smoke exposure, feeding canned tuna, canned cat food and cat foods with chemical additives, living in a rural environment, and having outdoor access as risk factors for FOSCC but there was no overlap in the risk factors between studies. In our study, risks for FOSCC were evaluated in an online epidemiologic survey study in 67 cats with FOSCC and 129 control cats. Clumping clay cat litter and flea collar use were significant risk factors for FOSCC on multiple logistic regression with odds ratios of 1.66 (95% CI 1.20–2.30) and 4.48 (95% CI 1.46–13.75) respectively. Crystalline silica is a carcinogen that may be present in all clay cat litters and tetrachlorvinphos is a carcinogen that is present in the most commonly used flea collars in our study. We recommend further investigation into the association between FOSCC and clay-based litter and/or flea collars containing tetrachlorvinphos. 相似文献
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Louise Katherine BARTOLAC Jenna Louise LOWE George KOUSTAS Cecilia SJ?BLOM Christopher Gerald GRUPEN 《The Journal of reproduction and development》2015,61(6):525-531
The aim of this study was to determine the optimum conditions for vitrifying in vitro
produced day 7 porcine embryos using different vitrification devices and blastocoele collapse methods. Firstly
embryos were collapsed by micro-pipetting, needle puncture and sucrose with and without conducting
vitrification. In the next experiment, non-collapsed embryos were vitrified in an open device using either
superfine open-pulled straws (SOPS) or the CryoLoopTM system, or vitrified in a closed device using
either the CryoTipTM or Cryo BioTM’s high security vitrification system (HSV). The
post-thaw survival of embryos vitrified in the open devices did not differ significantly (SOPS: 37.3%;
CryoLoopTM: 37.3%) nor did the post-thaw survival of embryos vitrified in the closed devices
(CryoTip™: 38.5%; HSV: 42.5%). The re-expansion rate of embryos that were collapsed via micro-pipetting
(76.0%) did not differ from those that were punctured (75.0%) or collapsed via sucrose (79.6%) when
vitrification was not performed. However, embryos collapsed via sucrose solutions (24.5%) and needle puncture
(16.0%) prior to vitrification were significantly less likely to survive vitrification than the control
(non-collapsed) embryos (53.6%, P < 0.05). The findings show that both open and closed vitrification
devices were equally effective for the vitrification of porcine blastocysts. Collapsing blastocysts prior to
vitrification did not improve survival, which is inconsistent with the findings of studies in other species.
This may be due to the extremely sensitive nature of porcine embryos, and/or the invasiveness of the
collapsing procedures. 相似文献