To what extent can multifractal measures provide an accurate model of the porosity of soils?

Over the last decades, several authors have suggested that multifractal measures, that is, self-similar measures defined on fractal or non-fractal objects, could be useful to describe soil properties, to model soil processes, and to deal with their extreme microscale heterogeneity. In this context, a key question relates to the extent to which multifractal measures can indeed fulfill all the expectations they have generated. To address this question, we discuss the possibility of generating a synthetic soil image exhibiting multifractal porosity. To this end, a simple geometrical multifractal model in 2D is developed, which helps us to better understand the concept of multifractality and to generate images. We show that it is possible to generate synthetic binary images over a limited range of scales, but that a pure multifractal model for the distribution of the solid or pore mass cannot be developed due to physical constraints. Moreover, in the generated images mimicking multifractal solid space, a higher degree of multifractality corresponds to a larger porosity, rendering it difficult to tune model parameters to match actual soil properties. In addition, simple statistics relying on power-law fits appear insufficient to characterize soil architecture even if they may capture some key multiscale indicators of observed spatial heterogeneity. We argue that the same conclusions would be reached in a three-dimensional space, as well as for grey-scales images.     

Co-localization of atmospheric H2 oxidation activity and high affinity H2-oxidizing bacteria in non-axenic soil and sterile soil amended with Streptomyces sp. PCB7

Two complementary experimental approaches were utilized to examine the extent to which free soil hydrogenases and H₂-oxidizing bacteria contribute to the soil uptake of atmospheric H₂. First, high affinity hydrogenase activity and H₂-oxidizing bacteria were fractionated in non-axenic soil and axenic soil colonized with the high affinity H₂-oxidizing bacterium Streptomyces sp. PCB7. Non-axenic soil was fractionated by buoyant density centrifugation. High affinity H₂ oxidation activity measured in individual fractions was proportional to the copy number of hhyL gene, specifying the large subunit of putative high affinity [NiFe]-hydrogenases. 2.5% of the hydrogenase activity was recovered in bacteria-free soil extract. Similarly, sequential centrifugation and wet filtrations of strain PCB7-colonized soil dispersed in solubilization buffer caused a loss of the activity, at a ratio proportional to the number of living cells removed. No abiontic hydrogenase activity was detected in bacteria-free fractions. The second experimental approach was designed to verify whether or not the [NiFe]-hydrogenase of strain PCB7 retains high affinity H₂ oxidation activity in soil, under the abiontic state. H₂ oxidation rates of crude enzyme extract of strain PCB7 measured under aerobic and anaerobic conditions were indistinguishable, indicating that the high affinity hydrogenase of strain PCB7 is oxygen-tolerant. The hydrogenase activity of sterile soil spiked with as much as 0.14 mg_(protein) g_(soil-dw)⁻¹ was equivalent to the H₂-oxidation activity of only 10⁶-10⁷ CFU of strain PCB7 g_(soil-dw)⁻¹. Taken together, our results indicate that high affinity hydrogenase activity is proportional to the abundance of H₂-oxidizing bacteria in soil and, that abiontic hydrogenases contribute only a few percent of the total high affinity H₂ oxidation activity detected in soil.     

Multisited Versus Compact Producer Service Firms: Insights from the French Case

Much of the literature on the location of producer services is concerned with their uneven spatial development across regions. Although this approach is useful, it proves to be limited, and a complementary analytical framework is required. This paper uses data from France's Annual Survey of Services to address producer service location from a novel perspective, that of the firm. A basic distinction is made between single site (compact) and multisite (network) producer service firms, and the natures of these two types are analyzed in terms of business strategies, location and performance. Further analyses are then based upon the distinctions between sales and servuction networks, and between specialized and diversified networks. Although significant for producing higher output levels, a network structure does not necessarily ensure a higher level of profitability.     

Enrichment of anhydrous milk fat in polyunsaturated fatty acid residues from linseed and rapeseed oils through enzymatic interesterification

Lipozyme TL IM was used in a solvent-free batch and microaqueous system for enzymatic interesterification of anhydrous milkfat (AMF) with linseed oil (LO) in binary blends and with rapeseed oil (RO) in one ternary blend. The aim was to obtain and characterize physicochemically fats enriched with unsaturated C 18 fatty acids (oleic, linoleic, and, especially, linolenic acids) from natural vegetable oils. Binary blends of AMF/LO 100/0, 90/10, 80/20, 70/30, and 60/40 (w/w) were interesterified. The change in triacylglycerol (TAG) profiles showed that quasi-equilibrium conditions were reached after 4-6 h of reaction. Free fatty acid contents <1%. The decrease in solid fat content and in dropping point temperature obtained with increasing content of LO and interesterification resulted in good plastic properties for the products originating from the blends 70/30 and 60/40. This was confirmed by textural measurements. Melting profiles determined by differential scanning calorimetry showed complete disappearance of low-melting TAGs from LO and the formation of intermediary species with a lower melting temperature. Oxidative stability of the interesterified products was diminished with increasing LO content, resulting in low oxidation induction times. A ternary blend composed of AMF/RO/LO 70/20/10 gave satisfactory rheological and oxidative properties, fulfilling the requirements for a marketable spread and, moreover, offering increased potential health benefits due to the enriched content in polyunsaturated fatty acid residues.     

Evaluating corrections for a horizontal offset between sensor and position data for surveys on land

When regarding evolutions in land-based, motorized sensor data collection it can be generalized that the speed of surveying, sampling rates, digital communications speed and survey resolutions have increased over the last years. Possibilities for accurate positioning have been following pace. However, a spatial offset between sensor and position data can be necessary to avoid interference with the instrument or can be the result of using a sensor array. It can also be due to practical considerations such as mounting possibilities. Unfortunately a severe degradation of positional accuracy is possible when performing corrections for a spatial offset and quantification of the induced error is quite difficult. As a consequence, the actual positional accuracy of sensor measurements is therefore often omitted or unknown, and a correction may be neglected during data processing. In this paper, accounting for a horizontal (spatial) offset is researched by examining the use of several correction methods. To evaluate the degree of loss of positional accuracy and validate several correction procedures, global navigation satellite system (GNSS) data (with real-time kinematic correction) have been simultaneously collected, using a GNSS receiver that was mounted on an all-terrain vehicle and two other receivers that were mounted near the front and end of an elongated sensor sled. The sled was connected to the towing vehicle using a flexible connection. Since the positioning systems’ horizontal accuracies were about 20 mm, it was possible to quantify the horizontal error of the predicted positions for the different correction procedures considered. The best approach for high-resolution surveys, which make use of a connection to a cart or sled that can rotate around a pivot on the towing vehicle, was researched. The strengths and weaknesses of the applied corrections were also evaluated, allowing selection of an appropriate correction for a given survey implementation.     

Hemocyte characteristics in families of oysters, Crassostrea gigas, selected for differential survival during summer and reared in three sites

High variability among individuals is often encountered when hemocyte characteristics are measured in bivalves. Such variability is suspected to result partly from genetic factors. In this study, hemocyte characteristics of six families of Crassostrea gigas were compared by flow cytometry at one sampling date in October 2001. These families were obtained from a nested, half-sibling cross design, and reared from July to October 2001 at three sites distributed along the French Atlantic coast from north to south: Baie des Veys (Normandy), Rivière d'Auray (Brittany) and Ronce (Marennes-oléron Basin, Poitou Charentes).

Among the 15 measured hemocyte characteristics, production of reactive oxygen species (ROS) of untreated hemocytes (maintained in filtered sterile seawater) and treated hemocytes (zymosan at 20 particles per hemocyte, and with Vibrio sp. S322 at 50 bacteria per hemocyte) was the most notable differences between families. This supports the existence of a genetic basis, at least partly, for the hemocyte characteristics of oysters, and especially for ROS production.

Among the six families analyzed, three have shown high survival during summer (named as “resistant”, mean mortality 5.2%) and three experienced high mortality during summer (named as “susceptible”, 30.6% mean mortality). Families showing high or low survival to summer mortality had similar hemocyte characteristics, regardless of the environmental conditions or reproductive state. Resistant families were observed to have higher total hemocyte counts and lower production of ROS than susceptible families. Moreover, ROS production of hemocytes from susceptible families was diminished significantly more by pathogenic Vibrio than that of resistant families. However, this study demonstrates also that rearing site strongly affected the hemocyte characteristics of all families of oysters, most notably hemocyte concentration and morphology (size and granularity), production of reactive oxygen species (ROS), and susceptibility to the cytotoxic activity of the pathogenic Vibrio sp. S322 (50 bacteria/hemocyte). Food availability and reproductive state are the most probable explanations for the site differences observed. Finally, it appeared difficult to link oyster survival during summer mortality to hemocyte profiles evaluated at one sampling date; other relevant indicators would probably help explaining oyster survival during summer mortality events.     

The rise of chemodiversity in plants

Plants possess multifunctional and rapidly evolving specialized metabolic enzymes. Many metabolites do not appear to be immediately required for survival; nonetheless, many may contribute to maintaining population fitness in fluctuating and geographically dispersed environments. Others may serve no contemporary function but are produced inevitably as minor products by single enzymes with varying levels of catalytic promiscuity. The dominance of the terrestrial realm by plants likely mirrored expansion of specialized metabolism originating from primary metabolic pathways. Compared with their evolutionarily constrained counterparts in primary metabolism, specialized metabolic enzymes may be more tolerant to mutations normally considered destabilizing to protein structure and function. If this is true, permissiveness may partially explain the pronounced chemodiversity of terrestrial plants.     

Varietal differences in the response of durum wheat (Triticum turgidum L. var. durum) to irrigation strategies in a semi-arid region of Algeria

The response of three durum wheat cultivars (C: Chen’s, V: Vitron, W: Waha) to irrigation was studied during 4 years in semi-arid Algeria (Chlef). The four treatments were NI (unirrigated), EI (early irrigation, up to heading), LI (late irrigation, from heading) and FI (full irrigation, over the entire season). FI increased rainfed grain yield (1,300 kg ha⁻¹) by 270%, EI by 107%, and LI by 67%. The variety × irrigation interaction was significant each year. Under irrigation, cv. Vitron was generally the most productive cultivar while in rainfed conditions cv. Waha always resulted in the highest grain yield. Grain yield increased exponentially with seasonal evapotranspiration (r ² = 0.741) and harvest index (r ² = 0.873). Water use efficiency for grain ranged from 4.6–5.3 kg ha⁻¹ mm⁻¹ (NI) to 9.6–10.8 kg ha⁻¹ mm⁻¹ (FI) as a function of cultivar and irrigation, cv. Vitron and cv. Waha (full irrigation) and cv. Waha (rainfed) being the most efficient cultivars. According to the evaporation pan method, the seasonal crop coefficient (K _c) values for the three cultivars were 0.64 (V), 0.62 (W) and 0.54 (C). The corresponding peak K _c values were 1.0, 0.97 and 0.89, respectively. K _c was closely related to leaf area index (LAI) and specific logarithmic relationships were calculated for each cultivar. Irrigation scheduling should be adapted to the type of cultivar in relation to its potential yield and LAI development pattern.     

Risk factors for Listeria monocytogenes contamination in French laying hens and broiler flocks

The objective of this study was to identify potential risk factors for Listeria monocytogenes contamination in French poultry production. Eighty-four flocks of layer hens kept in cages and 142 broiler flocks were included in this study. For each production type, a questionnaire was submitted to farmers and fecal samples were taken to assess the L. monocytogenes status of the flocks during a single visit to the farm. Two logistic regression models (specific to each production) were used to assess the association between management practices and the risk of L. monocytogenes contamination of the flock. The prevalence of L. monocytogenes-positive flocks was 30.9% (95% CI: 21.0; 40.9) and 31.7% (95% CI: 24.0; 39.4) for cage-layers and broiler flocks, respectively. For layer flocks, the risk of L. monocytogenes contamination was increased when pets were present on the production site. When droppings were evacuated by conveyor belt with deep pit storage, the risk of L. monocytogenes contamination decreased significantly. Feed meal was found to be associated with a higher risk of L. monocytogenes contamination than feed crumb. For broiler flocks, the risk of L. monocytogenes contamination was increased when farmers did not respect the principle of two areas (clean and dirty) at the poultry house entrance. A first disinfection by thermal fogging and the absence of pest control of the poultry house before the arrival of the next flock was found to increase the risk of contamination. When litter was not protected during storage and when farm staff also took care of other broiler chicken houses, the risk of L. monocytogenes contamination increased significantly. In the case of the watering system, nipples with cups were found to decrease the risk of contamination.