Verrucosamide,a Cytotoxic 1,4-Thiazepane-Containing Thiodepsipeptide from a Marine-Derived Actinomycete

A new cytotoxic thiodepsipeptide, verrucosamide (1), was isolated along with the known, related cyclic peptide thiocoraline, from the extract of a marine-derived actinomycete, a Verrucosispora sp., our strain CNX-026. The new peptide, which is composed of two rare seven-membered 1,4-thiazepane rings, was elucidated by a combination of spectral methods and the absolute configuration was determined by a single X-ray diffraction study. Verrucosamide (1) showed moderate cytotoxicity and selectivity in the NCI 60 cell line bioassay. The most susceptible cell lines were MDA-MB-468 breast carcinoma with an LD₅₀ of 1.26 µM, and COLO 205 colon adenocarcinoma with an LD₅₀ of 1.4 µM. Also isolated along with verrucosamide were three small 3-hydroxy(alkoxy)-quinaldic acid derivatives that appear to be products of the same biosynthetic pathway.     

疲劳/蠕变交互作用下竹木复合层合板断裂损伤研究

该文研究了在疲劳/蠕变交互作用下竹木复合层合板的断裂损伤行为.结果表明,在交变载荷最大值为竹木复合层合板破坏载荷的75%时,其疲劳/蠕变断裂曲线为三段式曲线;在交变载荷最大值为破坏载荷的55%时,6h内其疲劳/蠕变曲线为两段式曲线.随着蠕变时间的增加,层合板的断裂寿命降低.在交变载荷最大值为破坏载荷的75%时,蠕变时间为300s时,层合板的疲劳/蠕变负交互损伤程度大于蠕变时间为180s的.疲劳/蠕变交互作用下层合板的破坏形式主要为纤维撕裂、界面剪切破坏和层间开裂3种形式的综合表现.     

共同助推板式家具产业发展

据监测数据显示,2009年木质家具占全部家具的三分之一多,而板式家具产量占木家具产量比例接近80%。板式家具已确立了在我国家具产品中的主导地位。今后,随着中国城镇化及新农村建设进程的推进,     

鲜切生菜供应链温度波动与微生物含量变化

选取北京某优质农产品种植公司的6月中旬、12月下旬2条具有代表性的鲜切生菜供应链为研究对象,对整个链条温度波动规律分别进行为期7d的实地考察测量;并根据调研的温度曲线,在实验室条件下模拟供应链温度,测定鲜切生菜的微生物含量变化.研究结果显示,2条供应链温度都呈现出较大波动性：6月中旬实际供应链温度波动范围为在1.2～24.1℃,12月下旬为-6.1～16.2℃;运输过程温度波动较小,装车卸货等环节温度波动较明显.实验室模拟条件下得到的鲜切生菜微生物含量变化规律与供应链温度波动曲线存在正相关性,相关系数6月中旬0.849 77,12月下旬为0.847 99.     

2001年全球转基因作物种植情况

１９９６—２００１年的六年间，全球转基因作物种植面积增加了３０多倍，从１９９６年的１７０万hm２增加到了２００１年的５２６０万hm２。其中发展中国家为１３５０万hm２，发达国家转基因作物增加的种植面积（５６０万hm２）是发展中国家（２８０万hm２）的２倍。２００１年，四个国家占据了全球转基因作物种植面积的９９％，其中美国为３５７０万hm２（占６８％），阿根廷为１１８０万hm２（占２２％），加拿大３２０万hm２（占６％），中国１５０万hm２（占３％）。中国的年增长率最高，抗虫棉的种植面积从２０００年的５０万hm２增加…     

Ecological effects of climate fluctuations

Climate influences a variety of ecological processes. These effects operate through local weather parameters such as temperature, wind, rain, snow, and ocean currents, as well as interactions among these. In the temperate zone, local variations in weather are often coupled over large geographic areas through the transient behavior of atmospheric planetary-scale waves. These variations drive temporally and spatially averaged exchanges of heat, momentum, and water vapor that ultimately determine growth, recruitment, and migration patterns. Recently, there have been several studies of the impact of large-scale climatic forcing on ecological systems. We review how two of the best-known climate phenomena-the North Atlantic Oscillation and the El Ni?o-Southern Oscillation-affect ecological patterns and processes in both marine and terrestrial systems.     

Wheat grain quality under increasing atmospheric CO2 concentrations in a semi-arid cropping system

We investigated wheat (Triticum aestivum) grain quality under Free Air CO₂ Enrichment (FACE) of 550 ± 10% CO₂ μmol mol⁻¹. In each of two full growing seasons (2008 and 2009), two times of sowing were compared, with late sowing designed to mimic high temperature during grain filling. Grain samples were subjected to a range of physical, nutritional and rheological quality assessments. Elevated CO₂ increased thousand grain weight (8%) and grain diameter (5%). Flour protein concentration was reduced by 11% at e[CO₂], with the highest reduction being observed at the late time of sowing in 2009, (15%). Most of the grain mineral concentrations decreased under e[CO₂] - Ca (11%), Mg (7%), P (11%) and S (7%), Fe (10%), Zn (17%), Na (19%), while total uptake of these nutrients per unit ground area increased. Rheological properties of the flour were altered by e[CO₂] and bread volume reduced by 7%. Phytate concentration in grains tended to decrease (17%) at e[CO₂] while grain fructan concentration remained unchanged. The data suggest that rising atmospheric [CO₂] will reduce the nutritional and rheological quality of wheat grain, but at high temperature, e[CO₂] effects may be moderated. Reduced phytate concentrations at e[CO₂] may improve bioavailability of Fe and Zn in wheat grain.     

Effects of salinity on the growth and lipid composition of Atlantic salmon (Salmo salar) and turbot (Scophthalmus maximus) cells in culture

The direct effects of osmotic pressure (salinity) on growth performance and lipid composition were investigated in fish cells in culture. Cell lines from a relatively stenohaline marine species, turbot (Scophthalmus maximus) (TF) and an anadromous species, Atlantic salmon (AS) were cultured in media supplemented with NaCl to produce osmotic pressures varying from 300 to 500 mOsm kg⁻¹. The growth rates of the two cell lines were affected in a similar manner by the salinity of the media with the rank order for both peak cell numbers and growth rates up to the day of peak cell number being 300 > 350 > 400 > 450 > 500 mOsm kg⁻¹. Cell death occurred in both cell lines in older cultures at all salinities with the greatest loss of viable cells in media of 300 and 350 kg⁻¹. However, there were quantitative and qualitative differences between the cell lines in their lipid metabolism in response to the salinity of the media. The lipid content expressed per cell showed a positive correlation between lipid per cell and salinity in TF cells, but this was less apparent in AS cells. The percentage of total polar lipid classes increased with increasing salinity in TF cells due mainly to graded increases in the percentages of choline phospholipids. In contrast, there were no significant differences in the proportions of polar and neutral lipid classes with salinity in AS cells. The only significant effect of salinity in AS cells was a decreased proportion of dimethylacetals in total lipid at the highest salinity. The same significant effect of salinity on dimethylacetal content of total lipid was observed in TF cells. However, in addition there was a graded decrease in the percentage of 18:2n-9 in TF cell total lipid with increasing salinity. This was accompanied by increased percentages of total n-3 and n-6 PUFA with higher proportions of both groups of PUFA at 450 and 500 compared with 300 mOsm kg⁻¹. The results show that environmental salinity, in the absence of hormonal or other physiological stimuli, has direct effects on the growth and lipid metabolism of fish cells and that these effects differ in cells from different fish species.     

Dietary habits of grazers influence their suitability as biological controls of fouling macroalgae in ex situ mariculture

The dietary habits of the sea urchin Salmacis sphaeroides and the gastropod Trochus maculatus in ex situ mariculture were examined. Fouling algal assemblages on terracotta tiles were exposed to the grazers and compared. Except for Day 0, the assemblages on Days 2, 13 and 27 differed significantly across treatments. S. sphaeroides rapidly consumed most algae, leaving an assemblage dominated by turf algae and bare tile. T. maculatus primarily fed on green filamentous algae, resulting in an even distribution of other algal functional groups, while control tiles were dominated by green filamentous algae. Using three representative fouling algae species (Bryopsis corymbosa, Hypnea spinella and Lobophora variegata), the consumption rates of both grazers and dietary preferences of S. sphaeroides were examined through a single‐diet and a choice experiment respectively. The single‐diet experiment revealed differential algal consumption rates for S. sphaeroides (H. spinella = B. corymbosa > L. variegata) and T. maculatus (B. corymbosa > H. spinella = L. variegata). The choice experiment showed that S. sphaeroides preferred H. spinella over the other two species. These experiments highlighted the importance of understanding the dietary habits of grazers as this has direct implications on their effectiveness as biological controls of fouling macroalgae in mariculture.