Antimicrobial-induced endotoxin and cytokine activity in an in vitro model of septicemia in foals

OBJECTIVE: To determine which antimicrobials that are used to treat neonatal foals with septicemia attributable to Escherichia coli will minimize endotoxin release from bacteria and subsequent activity of inflammatory mediators while maintaining bactericidal efficacy. SAMPLE POPULATION: Blood samples from 10 healthy foals. PROCEDURE: Escherichia coli isolates A and B were isolated from 2 septicemic foals, and minimal inhibitory concentrations (MIC) were determined for 9 antimicrobials. Five of these antimicrobials were tested in vitro at 2 and 20 times their respective MIC. Whole blood or mononuclear cells grown in tissue-culture media were incubated with 105 colony-forming units of E. coli and each antimicrobial or saline (0.9% NaCl) solution. After 6 hours, number of viable bacteria remaining was determined, and supernatant was tested for endotoxin and tumor necrosis activity. RESULTS: Testing in whole blood was compromised by bactericidal effects of the blood itself. In mononuclear cell suspensions, each antimicrobial significantly reduced the number of viable bacteria to low or undetectable amounts. Antimicrobials did not differ significantly in efficacy of bacterial killing. Amikacin used alone or in combination with ampicillin resulted in significantly less endotoxin activity than did ampicillin, imipenem, or ceftiofur alone. There was a correlation between TNF-alpha and endotoxin activity. CONCLUSIONS AND CLINICAL RELEVANCE: Aminoglycosides appear less likely to induce endotoxemia and TNF-alpha synthesis during bactericidal treatment of E. coli septicemia, compared with beta-lactam antimicrobials. Use of ampicillin, imipenem, or ceftiofur in the treatment of septicemic neonatal foals should be accompanied by appropriate treatment for endotoxemia.     

Effects of cultivar resistance and fungicide application on stem canker of oilseed rape (Brassica napus) and potential interseasonal transmission of Leptosphaeria spp. inoculum

Phoma stem canker is a damaging disease of oilseed rape (Brassica napus) that causes annual yield losses to UK oilseed rape growers worth approximately £100 million, despite the use of fungicides. In the UK, oilseed rape is sown in August/September and harvested in the following July. The disease epidemics are initiated by ascospores released from Leptosphaeria spp. pseudothecia (ascocarps) on stem stubble in the autumn/winter. Control of this disease is reliant on the use of cultivars with “field resistance” and azole fungicides. This study investigated the effects of cultivar resistance and application of the fungicide prothioconazole on the severity of stem canker before harvest and the subsequent production of pseudothecia on the infected stubble under natural conditions in the 2017/2018, 2018/2019, and 2019/2020 cropping seasons. The application of prothioconazole and cultivar resistance decreased the severity of phoma stem canker before harvest, and the subsequent production of Leptosphaeria spp. pseudothecia on stubble in terms of pseudothecial density. Results showed that stems with less severe stem cankers produced fewer mature pseudothecia of Leptosphaeria spp. on the infected stubble. This investigation suggests that the most sustainable and effective integrated control strategy for phoma stem canker in seasons with low quantities of inoculum is to use cultivars with medium or good field resistance and apply only one spray of prothioconazole when required.     

Variation in diosgenin levels among 10 accessions of fenugreek seeds produced in western Canada

A collection of 10 accessions of fenugreek (Trigonella foenum-graecum L.), an annual legume, was grown during two summers at three plot locations in western Canada to assess whether genetic (accession) and environmental factors (site and year of production) influenced levels of diosgenin, a steroidal sapogenin. The 60 harvested seed samples, each analyzed by single determinations on three subsamples of defatted and dried seed material, were hydrolyzed by a microscale procedure in water containing 2-propanol (70%) and sulfuric acid (1 M). The extracts were analyzed by capillary gas chromatography with 6-methyldiosgenin as internal standard. Diosgenin levels from mature seeds ranged from 0.28 to 0.92% (28-92 microg/10 mg). Analysis of variance on combined diosgenin levels from the three sites and two years revealed that accession, accession x year, and site x year effects were significant for diosgenin content, whereas site, year, and site x accession effects were not. Four accessions, CN 19062, CN 19067, CN 19070, and CN 19071, were identified with high levels of diosgenin on the basis of the 2-year data set. In these accessions, mean levels of diosgenin plus yamogenin from seven site years were estimated at 0.70, 0.98, 0.84, and 0.87%, respectively.     

Topography of the northern hemisphere of Mercury from MESSENGER laser altimetry

Laser altimetry by the MESSENGER spacecraft has yielded a topographic model of the northern hemisphere of Mercury. The dynamic range of elevations is considerably smaller than those of Mars or the Moon. The most prominent feature is an extensive lowland at high northern latitudes that hosts the volcanic northern plains. Within this lowland is a broad topographic rise that experienced uplift after plains emplacement. The interior of the 1500-km-diameter Caloris impact basin has been modified so that part of the basin floor now stands higher than the rim. The elevated portion of the floor of Caloris appears to be part of a quasi-linear rise that extends for approximately half the planetary circumference at mid-latitudes. Collectively, these features imply that long-wavelength changes to Mercury's topography occurred after the earliest phases of the planet's geological history.     

Isolation of shiga-toxigenic Escherichia coli O157 from hide surfaces and the oral cavity of finished beef feedlot cattle

OBJECTIVE: To determine whether viable shiga-toxigenic Escherichia coli (STEC) O157 could be isolated from hide surface locations and the oral cavity of finished beef feedlot cattle. DESIGN: Within-animal prevalence distribution survey. ANIMALS: 139 finished cattle in 4 pens in a feedlot in Nebraska; prevalence of fecal STEC O157 shedding ranged from 20 to > 90%. PROCEDURE: Samples were collected from 7 sites from each animal: feces, oral cavity, and 5 hide surface locations (lumbar region, ventral aspect of the neck, ventral abdominal midline [ventrum], dorsal thoracic midline [back], and distal aspect of the left hind limb [hock]). RESULTS: Viable STEC O157 were isolated from the oral cavity or 1 or more hide surfaces of 130 cattle, including 50 fecal isolation-negative cattle. Site-specific prevalence of STEC O157 was 74.8% for oral cavity samples, 73.4% for back samples, 62.6% for neck samples, 60.4% for fecal samples, 54.0% for flank samples, 51.1% for ventrum samples, and 41.0% for hock samples. Only 5 cattle tested negative for STEC O157 at all 7 sites. Multiple correspondence and cluster analyses demonstrated that bacterial culture of feces, oral cavity samples, and back samples detected most cattle with STEC O157. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that viable STEC O157 may be isolated from the oral cavity, multiple hide surfaces, and feces of a high percentage of fed beef cattle and that bacterial culture of feces alone generally underestimates the percentage of fed beef cattle from which STEC O157 can be isolated.     

The effect of multi-nutrient fertilization on understory vegetation nutrient concentrations in inland Northwest conifer stands

This study looked at the effects that multi-nutrient fertilization had on understory vegetation nutrient concentrations at four conifer forested locations in the inland Northwest. Multi-nutrient fertilization of conifer stands cannot only enhance the overstory species in the inland Northwest but also the understory vegetation. Determination of nutrient concentration response to fertilization treatments can provide managers the ability to better manipulate their forests for grazing and wildlife habitat. We grouped the understory vegetation into three general life forms: forbs, grasses and grass-likes, and shrubs. Multi-nutrient fertilization had little effect on nitrogen concentration across all life forms. Potassium and sulfur generally increased in concentration. Micronutrients as a whole showed less variability in response to multi-nutrient fertilization. Boron, copper, molybdenum, and zinc generally showed increases in concentrations across all life forms. We were able to conduct analyses on a selected number of understory vegetation species. Individual species showed variability in nutrient concentration response to multi-nutrient fertilization. Wildlife habitat and grazing quality were both increased and decreased following multi-nutrient fertilization. Increases in nutrient concentrations will provide more nutritious vegetation to these animals and vice versa for decreases in concentrations.     

Determination of a tissue and sediment threshold for tributyltin to protect prey species of juvenile salmonids listed under the US Endangered Species Act

• 1. The purpose of this report is to determine the concentrations of tributyltin in sediments that would be protective against adverse effects on prey species of salmonids listed under the US Endangered Species Act.
• 2. Two approaches for determining adverse sediment concentrations due to tributyltin (TBT) contamination are presented here. The first is the equilibrium partitioning (EqP) approach, which relies on a sediment‐water partition coefficient and toxicological data for water exposures. The EqP approach utilizes the large water quality database that has been generated over the last two decades for TBT and provides strong evidence for adverse effects at low exposure concentrations.
• 3. The second approach involves determination of a TBT tissue residue that is considered harmful for most species, which is then used to predict the sediment concentration that would likely produce this adverse tissue concentration.
• 4. Both approaches are presented here because they generally support each other but based on the information presented below, and the inherent difficulty in measuring porewater concentrations, the tissue residue approach is the recommended method for determining adverse sediment concentrations.
• 5. Using this analysis, the protective sediment concentration for TBT proposed here is 6000 ng g^?1organic carbon. Direct effects are not expected on salmonids at this sediment concentration because of their relatively short residence time in the estuary, general lack of interaction with sediment, and relatively high metabolic capacity. This concentration may ensure adequate abundance of salmonid prey species; however, it may not be low enough for the protection of sensitive benthic species.

Published in 2002 by John Wiley & Sons, Ltd.     

Soil carbon sequestration with continuous no-till management of grain cropping systems in the Virginia coastal plain

Carbon sequestration in agroecosystems represents a significant opportunity to offset a portion of anthropogenic CO₂ emissions. Climatic conditions in the Virginia coastal plain and modern production practices make it possible for high annual photosynthetic CO₂ fixation. There is potential to sequester a substantial amount of C, and concomitantly improve soil quality, with the elimination of tillage for crop production in this region. The objectives of our research were to: (1) measure C sequestration rate with continuous no-till management of grain cropping systems of the Virginia middle coastal plain; (2) determine the influence of biosolids application history on C content and its interaction with tillage management; and (3) evaluate the impact of continuous no-till C stratification as an indicator of soil quality. Samples were collected from 63 sites in production fields using a rotation of corn (Zea mays L.)–wheat (Triticum aestivum L.) or barley (Hordeum vulgare L.)/soybean double-crop (Glysine max L.) across three soil series [Bojac (coarse-loamy, mixed, semiactive, thermic Typic Hapludults), Altavista (fine-loamy, mixed semiactive, thermic Aquic Hapludults), and Kempsville (fine-loamy, siliceous, subactive, thermic Typic Hapludults)] with a history of continuous no-till management ranging from 0 to 14 years. Thirty-two of the sites had a history of biosolids application. Five soil cores were collected at each site from 0–2.5, 2.5–7.5 and 7.5–15 cm and analyzed for bulk density and soil C. Bulk density in the 0–2.5 cm layer decreased and C stratification ratio (0–2.5 cm:7.5–15 cm) increased with increasing duration of continuous no-till due to the accumulation of organic matter at the soil surface. A history of biosolids application resulted in an increase of 4.19 ± 1.93 Mg C ha⁻¹ (0–15 cm). Continuous no-till resulted in the sequestration of 0.308 ± 0.280 Mg C ha⁻¹ yr⁻¹ (0–15 cm). Our results provide quantitative validation of the C sequestration rate and improved soil quality with continuous no-till management in the region using on-farm observations.     

Effect of irrigation amounts applied with subsurface drip irrigation on corn evapotranspiration, yield, water use efficiency, and dry matter production in a semiarid climate

Quantifying the local crop response to irrigation is important for establishing adequate irrigation management strategies. This study evaluated the effect of irrigation applied with subsurface drip irrigation on field corn (Zea mays L.) evapotranspiration (ETc), yield, water use efficiencies (WUE = yield/ETc, and IWUE = yield/irrigation), and dry matter production in the semiarid climate of west central Nebraska. Eight treatments were imposed with irrigation amounts ranging from 53 to 356 mm in 2005 and from 22 to 226 mm in 2006. A soil water balance approach (based on FAO-56) was used to estimate daily soil water and ETc. Treatments resulted in seasonal ETc of 580–663 mm and 466–656 mm in 2005 and 2006, respectively. Yields among treatments differed by as much as 22% in 2005 and 52% in 2006. In both seasons, irrigation significantly affected yields, which increased with irrigation up to a point where irrigation became excessive. Distinct relationships were obtained each season. Yields increased linearly with seasonal ETc (R² = 0.89) and ETc/ETp (R² = 0.87) (ETp = ETc with no water stress). The yield response factor (ky), which indicates the relative reduction in yield to relative reduction in ETc, averaged 1.58 over the two seasons. WUE increased non-linearly with seasonal ETc and with yield. WUE was more sensitive to irrigation during the drier 2006 season, compared with 2005. Both seasons, IWUE decreased sharply with irrigation. Irrigation significantly affected dry matter production and partitioning into the different plant components (grain, cob, and stover). On average, the grain accounted for the majority of the above-ground plant dry mass (≈59%), followed by the stover (≈33%) and the cob (≈8%). The dry mass of the plant and that of each plant component tended to increase with seasonal ETc. The good relationships obtained in the study between crop performance indicators and seasonal ETc demonstrate that accurate estimates of ETc on a daily and seasonal basis can be valuable for making tactical in-season irrigation management decisions and for strategic irrigation planning and management.     

Evaluation of flunixin meglumine as an adjunct treatment for diarrhea in dairy calves

OBJECTIVE: To assess the use of flunixin meglumine as an adjunct treatment for diarrhea in calves. DESIGN: Clinical trial. ANIMALS: 115 calves with diarrhea that were 1 to 21 days old at enrollment. PROCEDURE: Calves that developed diarrhea were randomly assigned to receive no flunixin meglumine (controls), a single dose of flunixin meglumine (2.2 mg/kg [1.0 mg/lb]), or 2 doses of flunixin meglumine administered 24 hours apart. Serum IgG concentration and PCV were measured prior to enrollment in the trial. Calves were evaluated daily to determine rectal temperature, fecal consistency, demeanor, and skin elasticity score. The primary analytic outcome was days of sickness (morbid-days). RESULTS: Calves with fecal blood and treated with a single dose of flunixin meglumine had fewer morbid-days and antimicrobial treatments, compared with controls. Although not significant, calves given 2 doses of flunixin meglumine in 24 hours had fewer morbid-days than untreated control calves. Regardless of severity of diarrhea, calves without fecal blood did not benefit from the use of flunixin. For calves with fecal blood, failure of passive transfer (low serum IgG concentration) was an independent risk factor for increased morbid-days. CONCLUSIONS AND CLINICAL RELEVANCE: Treatment with a single dose of flunixin meglumine resulted in fewer antimicrobial treatments and morbid-days in calves with fecal blood. As observed in other studies, calves with failure of passive transfer were at high risk for poor outcomes. This emphasizes the importance of developing and implementing effective colostrum delivery programs on dairy farms.