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51.
52.
Objective To determine whether a drug detected in the blood or urine of a racing animal could have penetrated through the skin from a topically applied preparation.
Design Blood and urine of dogs and horses were analysed after topical administration of three common nonsteroidal anti-inflammatory preparations.
Experimental method Dimethylsulphoxide was analysed using a gas chromatograph with a flame photometric detector. Phenylbutazone, its metabolites and lignocaine were analysed using a gas chromatograph with a mass selective detector.
Results Dimethylsulphoxide, phenylbutazone and ligno-caine were detected in dog urine after muliple applications of the preparations. The maximum concentration of dimethyl-sulphoxide in dog urine correlated with the concentration of dimethylsulphoxide in the preparation. Phenylbutazone penetrated the skin more effectively from the cream than from the solution or gel preparations. This penetration was independent of the concentration of dimethylsulphoxide.
Conclusion The superior penetration of phenylbutazone from the cream can be explained by it being present as a neutral molecule in an hydrophobic medium. It is proposed that phenylbutazone penetrates the skin of greyhounds most effectively by a hydrophobic lipid route which is likely to be different from the path by which dimethylsulphoxide penetrates the skin. 相似文献
Design Blood and urine of dogs and horses were analysed after topical administration of three common nonsteroidal anti-inflammatory preparations.
Experimental method Dimethylsulphoxide was analysed using a gas chromatograph with a flame photometric detector. Phenylbutazone, its metabolites and lignocaine were analysed using a gas chromatograph with a mass selective detector.
Results Dimethylsulphoxide, phenylbutazone and ligno-caine were detected in dog urine after muliple applications of the preparations. The maximum concentration of dimethyl-sulphoxide in dog urine correlated with the concentration of dimethylsulphoxide in the preparation. Phenylbutazone penetrated the skin more effectively from the cream than from the solution or gel preparations. This penetration was independent of the concentration of dimethylsulphoxide.
Conclusion The superior penetration of phenylbutazone from the cream can be explained by it being present as a neutral molecule in an hydrophobic medium. It is proposed that phenylbutazone penetrates the skin of greyhounds most effectively by a hydrophobic lipid route which is likely to be different from the path by which dimethylsulphoxide penetrates the skin. 相似文献
53.
AIM: To investigate the role of free-living animals such as spar- rows, rodents and flies as potential reservoirs of Campylobacter spp on a dairy farm, and to assess the genetic diversity among Campylobacter isolates from the farm and an urban source. METHODS: A total of 290 samples (bovine, passerine and ro- dent faeces, and whole flies) were collected from a large com- mercial dairy farm in the Manawatu district in New Zealand, and from faeces from urban sparrows in a nearby city. Other samples collected from the dairy farm included five from silage, two from aprons worn by workers during milking, two from workers' boots and two from water in troughs in a paddock. Isolates of thermophilic Campylobacter spp were identified mor- phologically and phenotypically and further characterised mo- lecularly using pulsed-field gel electrophoresis (PFGE) and the restriction enzyme SmaI. RESULTS: Campylobacter jejuni was the only Campylobacter species isolated from all samples. The highest prevalence was found in faeces from dairy cows (54%), followed by faeces from sparrows from the urban area (40%) and the farm (38%), and from rodents (11%) and whole flies (9%). Other samples from the farm environment such as silage, trough water, and work- ers' aprons and boots were also positive for C. jejuni. Of the 22 restriction patterns obtained, seven were common to more than one source. CONCLUSIONS: Cattle, sparrows, rodents and flies are po- tential reservoirs of C. jejuni on dairy farms. Identical clones of C. jejuni carried by cattle, sparrows, flies and rodents prob- ably indicate a common source of infection. The high level of asymptomatic carriage of C. jejuni by healthy dairy cows could be sufficient to maintain infections within the dairy farm sur- roundings via environmental contamination. 相似文献
54.
J. H. Jakobsen P. Madsen J. Jensen P. H. Petersen G. Aa. Pedersen 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2013,63(1):39-46
In a breeding programme where young potential breeding bulls are reared on performance test stations, selection based on own results can be carried out before test inseminations. Both beef and milk production traits are included in the total merit index used for selection, and estimates of genetic and phenotypic parameters of these traits are therefore of interest for an optimal construction of such indices. Data on first lactation milk records from the field and beef records of potential dairy breeding bulls from the Danish performance test stations were analysed in bivariate animal-sire models using the AI-REML algorithm. Genetic correlations of 0.16, 0.25 and 0.43 between feed intake capacity and protein yield were obtained for Red Danish (RD), Danish Black and White (DBW) and Danish Jersey (DJ), respectively. These correlations were significantly different from zero for the two populations (DBW and DJ). Genetic correlations around zero between feed efficiency and protein yield were obtained for all three populations. Genetic correlations of 0.44, 0.19 and 0.47 between average daily gain and protein yield were obtained for RD, DBW and DJ, respectively. The genetic correlations between protein yield and muscle area was close to zero for DBW, while it was -0.31 for RD. Selection index calculations indicate that indices composed of different beef performance traits can be used as early predictors for milk yield. Selection on such an index could increase the breeding value of the young bulls for milk production traits by 0.8-2.0% of the population mean. 相似文献
55.
Russell J. S. Orr Anke Stüken Shauna A. Murray Kjetill S. Jakobsen 《Marine drugs》2013,11(8):2814-2828
Numerous species of marine dinoflagellates synthesize the potent environmental neurotoxic alkaloid, saxitoxin, the agent of the human illness, paralytic shellfish poisoning. In addition, certain freshwater species of cyanobacteria also synthesize the same toxic compound, with the biosynthetic pathway and genes responsible being recently reported. Three theories have been postulated to explain the origin of saxitoxin in dinoflagellates: The production of saxitoxin by co-cultured bacteria rather than the dinoflagellates themselves, convergent evolution within both dinoflagellates and bacteria and horizontal gene transfer between dinoflagellates and bacteria. The discovery of cyanobacterial saxitoxin homologs in dinoflagellates has enabled us for the first time to evaluate these theories. Here, we review the distribution of saxitoxin within the dinoflagellates and our knowledge of its genetic basis to determine the likely evolutionary origins of this potent neurotoxin. 相似文献
56.
Abstract AIM: To examine the effect of setting a maximum milking time, from peak lactation until drying-off, on production, duration of milking, and udder health of dairy cows. METHODS: Forty cows were assigned in twin-pairs to be either milked until cups were removed at a milk flow-rate threshold of 0.35 kg/minute (Control), or until cups were removed at a milk flow-rate threshold of 0.35 kg/minute, or maximum time, whichever came first (MaxT). The maximum time was set by determining the milking time of the 70th percentile cow when ranked from fastest to slowest, irrespective of yield. The milking routine was typical of that practised on dairy farms in New Zealand, and involved no pre-milking preparation. The study began at peak lactation (68 (SD 7) days in milk; DIM) and continued for 26 weeks. Duration of milking and milk yield were measured for each milking. Composition of milk was determined from weekly herd tests, and milk quality from fortnightly somatic cell counts (SCC). Completeness of milking and teat condition were assessed during the study. The bacterial status of quarter milk samples was determined at the beginning and end of the study, and all treated cases of clinical mastitis recorded. ANOVA was used to examine the effect of treatment group on variables of interest. RESULTS: Total milk, fat and protein yields during the study period did not differ between treatments. On average, 30.3% of the morning and 27.6% of the afternoon milkings of MaxT cows reached the maximum time at which cups were removed, and were therefore shortened. While the average milking time of the slowest-milking cow was longer for the Control compared with MaxT group in Weeks 1–18, the average milking time did not differ between treatments. There was no difference in overall SCC, and the incidence of clinical mastitis, or the percentage of infected quarters at drying-off, was similar for the MaxT and Control cows. CONCLUSION: The results show that setting a maximum milking time can reduce the milking time of slower-milking cows in a herd without compromising overall herd production and udder health. CLINICAL RELEVANCE: Although the numbers of cows in the study were small there was no evidence of a major increase in SCC, or subclinical or clinical mastitis when a maximum milking time was set for slower-milking cows. 相似文献
57.
Inorganic nitrogen addition in a semi‐intensive turbot larval aquaculture system: effects on phytoplankton and zooplankton composition 下载免费PDF全文
Elisa Blanda Benni Winding Hansen Jacob Kring Højgaard Per Meyer Jepsen Morten Foldager Pedersen Thomas Allen Rayner Christina Vinum Thoisen Hans Henrik Jakobsen 《Aquaculture Research》2016,47(12):3913-3933
A nitrogen manipulation experiment was conducted in a semi‐intensive outdoor system where turbot larvae feed on copepods. Nitrogen addition is hypothesized to stimulate a cascade reaction increasing phytoplankton biomass, copepods’ productivity and larval fish survival. Triplicates were established for three treatments: a control with no additional nitrogen, a pulsed dose where nitrogen was added in three doses over time and a full dose where all nitrogen, equal to the total of the pulse dose treatment, was added initially. In the control, chlorophyll a averaged 3.3 ± 1.5 μg L?1 and phytoplankton was dominated by diatoms, while the pulsed and full dose treatments showed chlorophyll a at 28.6 ± 9.9 and 47.7 ± 10.0 μg L?1, respectively, with dinoflagellates as the main phytoplankton group. Due to photosynthesis, pH increased >9 in both the nitrogen treatments compared to the control (8.5). Potential toxic dinoflagellates, including Alexandrium pseudogonyaulax and Prorocentrum spp., became dominant in the nitrogen treatments and might have arrested zooplankton recruitment. Laboratory experiments with a toxic strain of A. pseudogonyaulax proved that Acartia tonsa reproduction and naupliar survival were affected negatively at realistic fish tank concentrations of 100 and 20 cells mL?1, respectively. Compared to the control, pulsed and full dose treatments reached higher copepod biomass and showed a shift over time in species composition from Centropages hamatus to Acartia spp. However, high pH levels and dinoflagellate blooms had a negative effect on larval fish survival, suggesting management improvements on water quality and separation between copepods and fish production tanks. 相似文献
58.
Xia Ge D André d'Avignon Joseph JH Ackerman R Douglas Sammons 《Pest management science》2010,66(4):345-348
BACKGROUND: Glyphosate‐resistant (GR) weed species are now found with increasing frequency and threaten the critically important GR weed management system. RESULTS: The reported 31P NMR experiments on glyphosate‐sensitive (S) and glyphosate‐resistant (R) horseweed, Conyza canadensis (L.) Cronq., show significantly more accumulation of glyphosate within the R biotype vacuole. CONCLUSIONS: Selective sequestration of glyphosate into the vacuole confers the observed horseweed resistance to glyphosate. This observation represents the first clear evidence for the glyphosate resistance mechanism in C. canadensis. Copyright © 2010 Society of Chemical Industry 相似文献
59.
The free modulation of interlayer distance in a layered high-transition temperature (high-Tc) superconductor is of crucial importance not only for the study of the superconducting mechanism but also for the practical application of high-Tc superconducting materials. Two-dimensional (2D) superconductors were achieved by intercalating a long-chain organic compound into bismuth-based high-Tc cuprates. Although the intercalation of the organic chain increased the interlayer distance remarkably, to tens of angstroms, the superconducting transition temperature of the intercalate was nearly the same as that of the pristine material, suggesting the 2D nature of the high-Tc superconductivity. 相似文献
60.