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81.
The present and future of withdrawal period calculations for milk in the European Union: dealing with data below the limit of quantification 下载免费PDF全文
J. Henri A.‐M. Jacques P. Sanders A. Chevance M. Laurentie 《Journal of veterinary pharmacology and therapeutics》2017,40(2):116-122
The assessment of withdrawal periods for milk is affected by the occurrence of data below the lower analytical quantification limit (BLQ data) and the resulting uncertainty. The current regulatory approach for dealing with BLQ residues is simple and easy: BLQ data (and missing data) are arbitrarily reassigned a value of one‐half the LOQ before any calculation on the data with one of the three currently applicable methods. Here, we reconsider the determination of the withdrawal period of milk with data below the limit of quantification. Theoretical background on analytical limits and pharmacometric considerations will be established. Then, we analyze the uncertainty problems caused by the current approach and propose a calculation solution (maximum‐likelihood estimation handling left‐censored data) included in nonlinear mixed‐effects modeling. Finally, we illustrate this issue using a case example. 相似文献
82.
Adrian Muwonge Clovice Kankya Jacques Godfroid Berit Djonne John Opuda-Asibo Demelash Biffa Takele Ayanaw Musso Munyeme Eystein Skjerve 《Tropical animal health and production》2010,42(5):905-913
To date, the public health relevance of mycobacterial infections in pigs is not well investigated despite high risk of infection.
Recently, there has been a documented increase in opportunistic infections and risk of acquiring opportunistic mycobacterial
infections in HIV/AIDS patients in Mubende district; unfortunately, there has been no published information on the epidemiology
of mycobacterial infections in this area. This study was carried out between September 2008 and February 2009. Investigations
were done to assess the prevalence and associated risk factors of mycobacterial infections in slaughtered pigs in Mubende
district of Uganda. A total of 997 pigs (53.7% male and 46.3% female) from 31 different slaughterhouses were examined for
the presence of lesions compatible with TB and mycobacterial infections. Pathologic tissue specimens were collected for culturing
and isolation of mycobacteria. A cross-sectional technique was used based on convenient visits to slaughterhouses but random
selection of individual slaughtered pigs for a detailed post-mortem inspection on a daily basis. The results reflected a 9.3%
and 3.1% (95% CI) prevalence of Mycobacterium species based on necropsy examinations and culture isolation, respectively. The highest prevalence of mycobacterial infection
was recorded in Buwekula County (the mixed agro-zone) whilst the lowest was in Kassanda County (pastoral zone). A multivariable
logistical regression analysis identified age (P ≤ 0.001) and sex (P ≤ 0.05) as risk factors for mycobacterial infections in pigs. Post-estimation statistics of the regression model evaluation
and validation fit it well into the data (HL, χ
2 = 5.9; P = 0.69 for necropsy, HL χ
2 = 2.9; P = 0.94 for culturing). This study documented a high prevalence of mycobacterial infections in slaughter pigs in Mubende district.
The fact that pigs and human often share common housing and environment poses a high risk of zoonotic transmission. This then
warrants further molecular investigation to identify the specific Mycobacterium species and their public health importance in this area. 相似文献
83.
Jean-Paul Laclau Joseph Levillain Philippe Deleporte Jean de Dieu Nzila Jean-Pierre Bouillet Laurent Saint André Antoine Versini Louis Mareschal Yann Nouvellon Armel Thongo M’Bou Jacques Ranger 《Forest Ecology and Management》2010
Tropical plantation forests are meeting an increasing proportion of global wood demand and comprehensive studies assessing the impact of silvicultural practices on tree and soil functioning are required to achieve sustainable yields. The objectives of our study were: (1) to quantify the effects of contrasting organic residue (OR) retention methods on tree growth and soil nutrient pools over a full Eucalyptus rotation and (2) to assess the potential of soil analyses to predict yields of fast-growing plantations established on tropical sandy soils. An experiment was set up in the Congo at the harvesting of the first rotation after afforestation of a native herbaceous savanna. Six treatments were set up in 0.26 ha plots and replicated in 4 blocks, with OR mass at planting ranging from 0 to 46.5 Mg ha−1. Tree growth over the whole rotation was highly dependent on OR management at planting. Over-bark trunk volume 7 years after planting ranged from 96 m3 ha−1 in the treatment with forest floor and harvest residue removal at planting to 164 m3 ha−1 in the treatment with the largest amount of OR. A comparison of nutrient stocks within the ecosystem at planting and at the end of the rotation suggested that nutrient contents in OR were largely involved in the different response observed between treatments. OR management treatments did not significantly modify most of the nutrient concentrations in the upper layers of the mineral soil. Conventional soil analyses performed before planting and at ages 1 and 3 years were unable to detect differences between treatments despite large differences in tree growth. In contrast, linear regressions between stand aboveground biomass at harvesting and OR mass at planting (independent variable) showed that OR mass was an excellent predictor of stand yield (R2 = 0.99). A large share of soil fertility comes from organic material above the mineral soil in highly weathered sandy soils and OR mass at planting might be used in conjunction with soil analyses to assess the potential of these soils to support forest plantations. 相似文献
84.
Céline Josso Anne Le Ralec Lucie Raymond Julia Saulais Jacques Baudry Denis Poinsot Anne Marie Cortesero 《Landscape Ecology》2013,28(9):1697-1715
Agriculture intensification has deeply modified agroecosystems from field to landscape scales. To achieve successful pest control using natural enemies, understanding species interactions over all scales remains a challenge. Using the cabbage root fly as a model, we studied whether field and landscape characteristics influenced colonization and infestation of broccoli fields by the pest and its control by natural enemies. We also determined whether species of different trophic level or host specialization would respond to environmental characteristics at the same spatial extent. During a multiple-species and multiple-spatial extent study in northwestern France, we recorded pest colonization and infestation in 68 fields, collected associated natural enemies and assessed crop damages. In each field, we considered management practices and characterized the surrounding landscape in 50–500 m-wide buffers. Our main findings are that Delia radicum and its main natural enemies respond to both field and landscape characteristics. Semi-natural areas supported both crop colonization by pests and natural enemy action. The pest and its enemies differed in their responses to field or landscape variables. Landscape elements such as field banks favored the movement of the pest while impeding the movement of some natural enemies. Pest pressure did not increase with the neighboring density of Brassica crops. The presence of natural enemies did not reduce crop damage but reduced pest emerging rates. Finally, specialist parasitoids responded to the landscape at larger spatial extents than generalists. These results outline the complexity of improving pest control through landscape management. 相似文献
85.
86.
Glanzmann thrombasthenia (GT) is characterized by a defect of platelet aggregation. This autosomal recessive genetic disorder is caused by an abnormality of the platelet glycoprotein receptors alpha IIb or beta III. Recently, we identified a horse with clinical and pathological features of GT. The aim of this study was to describe this case of GT at the molecular level. A point mutation from G to C in exon 2 of ITGA2B causing a substitution of the expected amino acid arginine 72 (Arg(72)) by a proline (Pro(72)) was encountered. This amino acid change may result in abnormal structural conformations that yield an inactive alpha IIb subunit. The genomic DNA analysis showed that this horse was homozygous for the missense mutation. 相似文献
87.
van Beurden SJ Leroy B Wattiez R Haenen OL Boeren S Vervoort JJ Peeters BP Rottier PJ Engelsma MY Vanderplasschen AF 《Veterinary research》2011,42(1):105
ABSTRACT: Many of the known fish herpesviruses have important aquaculture species as their natural host, and may cause serious disease and mortality. Anguillid herpesvirus 1 (AngHV-1) causes a hemorrhagic disease in European eel, Anguilla anguilla. Despite their importance, fundamental molecular knowledge on fish herpesviruses is still limited. In this study we describe the identification and localization of the structural proteins of AngHV-1. Purified virions were fractionated into a capsid-tegument and an envelope fraction, and premature capsids were isolated from infected cells. Proteins were extracted by different methods and identified by mass spectrometry. A total of 40 structural proteins were identified, of which 7 could be assigned to the capsid, 11 to the envelope, and 22 to the tegument. The identification and localization of these proteins allowed functional predictions. Our findings include the identification of the putative capsid triplex protein 1, the predominant tegument protein, and the major antigenic envelope proteins. Eighteen of the 40 AngHV-1 structural proteins had sequence homologues in related Cyprinid herpesvirus 3 (CyHV-3). Conservation of fish herpesvirus structural genes seemed to be high for the capsid proteins, limited for the tegument proteins, and low for the envelope proteins. The identification and localization of the structural proteins of AngHV-1 in this study adds to the fundamental knowledge of members of the Alloherpesviridae family, especially of the Cyprinivirus genus. 相似文献
88.
Kevers C Pincemail J Tabart J Defraigne JO Dommes J 《Journal of agricultural and food chemistry》2011,59(11):6165-6171
Apple and pear fruits are important sources of secondary plant metabolites and one of the major sources of dietary phenolics consumed all year round. The aim of this work was to identify the main variables influencing phenolic content and antioxidant capacity in apples. Higher phenolic and antioxidant contents were observed in some varieties (such as the Delbar Estival apple and Durondeau pear). Storage conditions were important. Our results also showed that fruits should be consumed rapidly after purchase and with their peel. After one week of domestic storage, the ascorbic acid content was found to decrease by 75%. Peeling led to a more than 25% decrease in total phenolics and ascorbic acid. The harvest time (at normal ripeness) had only a limited impact, but significant year-to-year variations were observed. In conclusion, well-chosen and well-stored apples and pears may contribute to an antioxidant-rich diet if consumed rapidly and with their peel. 相似文献
89.
Megersa B Biffa D Abunna F Regassa A Godfroid J Skjerve E 《Tropical animal health and production》2011,43(3):651-656
The involvement of Brucella infection in causing abortion was investigated in a breeding female subpopulation of 283 cattle, 756 camels, and 757 goats.
Serum samples were serially tested using the Rose Bengal test and complement fixation test. The study showed that anti-Brucella antibodies were prevalent in 10.6% (95% confidence interval (CI), 7.4, 14.9), 2.2% (95%CI, 1.4, 3.7), and 1.9% (95%CI, 1.1,
3.2) of cattle, camel, and goats, respectively. Abortion was more commonly reported in camels (23.4%) than cattle (13.8%)
and goats (12.4%). The results of this study suggested that Brucella infections contribute significantly to abortion in cattle (odds ratio (OR), = 4.7; 95%CI, 2.0, 10.8) and goats (OR = 6.9;
95%CI, 2.2, 21.7) but not in camels. The number of young animals produced by breeding females seems to be apparently reduced
in seropositive groups. Keeping more than two animal species at household level was found to be the risk factor for cattle
(OR = 3.1; 95%CI, 1.2, 7.9) and camel (OR = 5.3; 95%CI, 1.2–23.5) seropositivity to Brucella infection when compared to those animals from households that keep only two animal species. This may suggest a possibility
of cross species transmission of Brucella infection under such mixed herding. Wet season (OR = 4.8; 95%CI, 1.3, 18.1) was found to be associated with seropositivity
in goats, linked to a coincidence of increased deliveries in flocks with possible excretion of Brucella organisms. The study results suggest that Brucella infection is the likely cause of abortion in cattle and goats while other causes largely outweigh brucellosis as a cause
of abortion in camels in Borana, hence, contributing to reproductive loss. 相似文献
90.
Staphylococcus aureus is recognized worldwide as a pathogen causing many serious diseases in humans and animals, and is the most common aetiological agent of clinical and subclinical bovine mastitis. The importance of evaluating the combination of S. aureus virulence factors has been emphasized both in human and veterinary medicine, and knowledge about the genetic variability within different S. aureus populations would help in the design of efficient treatments. The aim of the present study was to determine the genetic profiles of S. aureus strains isolated from milk of cows suffering from clinical and subclinical mastitis in Belgium. The presence of about forty virulence-associated genes was investigated by specific polymerase chain reaction (PCR) amplification. A high number of genotypic subtypes were observed, demonstrating further the large variation in the presence of virulence genes in S. aureus isolates and the considerable diversity of strains populations that are able to cause mastitis in cows. In accordance with other studies, we showed that some genes are associated with mastitis-causing S. aureus isolates, whereas others are absent or rarely present. We also further highlighted the presence of conserved gene combinations, namely the enterotoxigenic egc-cluster and the bovine pathogenicity island SaPIbov. Importantly, the presence of isolates carrying genes coding for toxins involved in important human infections makes the milk of cows with mastitis a potential reservoir for these toxins, and therefore a potential danger in human health, which strengthens the importance to consider raw milk consumption and its processing very carefully. 相似文献