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Groups of 8 steers weighing 300–400 kg (660–880 Ib) were rotationally grazed on a ryegrass-dominant pasture for 115 days. The systems compared were zero grazing (Z), field grazing conducted at the same stocking rate (FC), and field grazing conducted at a stocking rate varied with the intention of giving the same liveweight gain per uiimal as zero grazing (FV). Mean daily liveweight gains were: Z, 0.98; FC, 0.78; FV, 0.90 kg/animal (2.2, 1.7 and 20 Ib/animal), and liveweight gains/unit area were in the ratio 100:78:85. Organic-matter intake, measured on four occasions, was, on average, Z, 6.54; FC, 6.18; FV, 687 kg/head daily (14.4, 13.6 and 15.1Ib). From these results it appears that a comparison of zero grazing and field grazing made at the same stocking rate is likely to underestimate the potential of field grazing for beef production from grass. 相似文献
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A new monodesmosidic spirostanol saponin, along with three known saponins was isolated from Agave macroacantha Zucc leaves. The structure of the new saponin was established as hecogenin-3-O-α-l-rhamnopyranosyl (1→4) β-d-xylopyranosyl (1→3)[β-d-glucopyranosyl (1→2)] β-d-glucopyranosyl (1→4) β-d-galactopyranoside. The 1H and 13C resonances of the four compounds were assigned using a combination of 1D and 2D NMR techniques including 1H, 13C, COSY, TOCSY, ROESY, HSQC and HMBC NMR and confirmed by mass spectrometry. 相似文献
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Christley RM Hodgson DR Rose RJ Hodgson JL Wood JL Reid SW 《The Veterinary record》2001,148(4):99-104
A matched case-control study was made of 100 thoroughbred horses which were coughing and 148 control horses which were free of clinical signs of respiratory tract disease. The variables identified by multivariable conditional logistic regression as being significantly associated with coughing included age (the risk decreased with age), the stage of training (horses in early training were at greatest risk), the time since the last race (horses that had never raced were at greatest risk) and the time since they were last transported (horses transported more than 14 days previously were more likely to cough than those transported within the last week). The coughing horses were significantly more likely to have high scores for upper and lower tracheal mucus and pharyngeal lymphoid hyperplasia. In addition, the tracheal aspirates of the coughing horses had increased odds of neutrophilia and were more likely to have intracellular bacteria than the control horses. However, a considerable proportion of the control horses had cytological and/or endoscopic evidence of airway inflammation. 相似文献
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Overestimation of canine albumin concentration with the bromcresol green method in heparinized plasma samples 总被引:5,自引:0,他引:5
Stokol T Tarrant JM Scarlett JM 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2001,30(4):170-176
Albumin concentrations are routinely measured in dogs with bromcresol green (BCG)-binding assays on automated chemistry analyzers. Several variables affect this assay, including the length of reaction time, sample type, and lack of specificity of BCG for albumin. We observed that albumin concentrations measured with BCG appeared higher in heparinized plasma samples in sick dogs. The objective of this study was to determine the effect of anticoagulant and assay procedure on BCG albumin concentrations in clinically ill dogs. We hypothesized that albumin concentrations would be overestimated in heparinized plasma compared with serum because of the combination of heparin and fibrinogen. Furthermore, we hypothesized that the overestimation would be influenced by assay parameters. Blood was collected from 32 clinically ill dogs into tubes containing heparin, citrate, or no anticoagulant. Citrate was chosen to assess the effect of fibrinogen in the absence of heparin. Albumin concentration was measured in all 3 sample types from each dog using 2 different BCG procedures on an automated chemistry analyzer. The BCG procedures (standard and modified) differed in the wavelengths used for absorbance readings (standard, 600/700; modified, 570/505) and the time point at which absorbance was measured (standard, 100 seconds; modified, 40 seconds). In addition, the modified method incorporated a sample blank. Globulin fractions, fibrinogen concentration, and indices of lipemia, hemolysis, and icterus were evaluated for their contribution to the overestimation of albumin concentration in heparinized plasma compared with serum samples. Albumin concentrations were significantly higher (P =.05) in heparinized plasma (mean +/- SE, 3.8 +/- 0.1 g/dL) than in serum (3.6 +/- 0.2 g/dL) or citrated plasma (3.2 +/- 0.1 g/dL). Overestimation was evident only with the standard BCG procedure. Multiple linear regression analysis indicated that fibrinogen was largely responsible for the higher albumin concentration in heparinized plasma with the standard method. Based on these results, heparin is not recommended as an anticoagulant for albumin measurement in dogs when using a BCG method unless the assay uses a sample blank and a reaction time of <1 minute. 相似文献