Measurement of canine plasma fibronectin by rocket immunoelectrophoresis

Plasma fibronectin concentrations of 148 normal canine samples were measured by rocket immunoelectrophoresis. Electrophoresis was accomplished, using 2.0% rabbit anticanine fibronectin by volume in 0.7% agarose in buffer. Films were electrophoresed 18 hours in barbital buffer, 7.5 mA/film. The mean fibronectin concentration for normal citrated dog plasma was 290 micrograms/ml +/- 50 micrograms/ml.     

Hypoalbuminemia-related platelet hypersensitivity in two dogs with nephrotic syndrome

Platelet aggregation studies in 2 dogs with nephrotic syndrome disclosed increased platelet sensitivity to a low dose of adenosine diphosphate. Subsequent studies with isolated platelets and plasma indicated that a plasma factor was responsible primarily for inducing platelet hypersensitivity. The increased platelet aggregation response was corrected by increasing the albumin concentration of the plasma. The study suggested an important role for albumin in modulating platelet aggregation and may partially explain the tendency toward thrombosis noted in hypoalbuminemic dogs with nephrotic syndrome.     

Imaging: Nuclear*

In this paper an overview of principles involved in nuclear imaging is presented. Nuclear images are picotrial representations of the distribution of systemically administered radiopharmaceuticals. Although many radionuclides are available. ^99mTc is the most commonly used radionuclide, either in ionic form (^99mTcO₄) or bound to other pharmaceuticals. Distribution within the body is by physiologic processes, dependent on the radiopharmaceutical used. Most distribution processes are organ specific rather than disease specific. Scintillation detectors are used to record the activity and disribution of an administered radiopharmaceutical and to create a representative image on film. A rectilinear scanner is an imaging system with a small detector that moves systematically, point by point, over an organ of interest. A gamma (scintillation) camera is an imaging system with a large stationary detector that encompasses a larger field of view. Nuclear imaging provides information based primarily on physiologic principles, and radiography provides infromation based primarily on anatomic or geometric principles. Examples of some scanning procedures are discussed.     

Leptospirosis in Colombia: Isolation ofLeptospira SPP. from the kidneys of brown rats (Rattus Norvegicus) trapped on infected piggeries

Summary Leptospira interrogansserotypes pomonaand icterohaemorrhagiaewere isolated from the kidneys of Rattus norvegicustrapped on pomonainfected piggeries.The isolation of pomonafrom one of 111cultured kidneys, although of interest, strongly suggests that this rat does not play an important part in the epidemiology of infection by this serotype. Kidney tubular degeneration, focal mononuclear infiltrations and fibroplasia are morphological traces which remain after an attack of leptospirosis. The high percentage (48.6)of these lesions found in the rat kidneys examined does not seem to be related to serotype pomonabut to icterohaemorrhagiae.The importance of this last serotype in swine in the Cauca Valley has yet to be assessed.

---

Leptospirosis En Colombia: Aislamiento DeLeptospira SPP. De Riñones De Ratas Pardas (Rattus Norvegicus) Atrapadas En Porquerizas Infectadas

Resumen Se aislóLeptospira interrogans serotipospomona eicterohaemorrhagiae de riñones deRattus norvegicus atrapadas en porquerizas infectadas porpomona. El aislamiento depomona de 1/111 riñones cultivados, aunque de interés, sugiere que esta rata no desempeña parte importante en la epidemiología de las infecciones por este serotipo.Degeneración tubular renal, infiltración mononuclear focal y fibroplasia son huellas morfológicas que quedan después de un ataque de leptospirosis. El alto porcentaje (48.6) de estas lesiones encontradas en los riñones de las ratas examinadas no parece estar relacionado con el serotipopomona sino con elicterohaemorrhagiae. La importancia de este último seroptipo en cerdos en el Valle del Cauca no se ha evaluado todavía.

---

Leptospiroses En Colombie: Isolement DeLeptospira SPP. De Reins De Rats Bruns (Rattus Norvegicus) Captures Dans Des Porcheries

Résumé Les sérotypespomona eticterohaemorrhagiae deLeptospira interrogans ont été isolés de reins deRattus norvegicus capturés dans des porcheries infectées de (pomona?). L'isolement depomona sur une seule des 111 cultures de rein effectuées, bien qu'interessant, suggère fortement que ce rat ne joue pas un rôle important dans l'épidémiologie de l'infection par ce sérotype.La dégénerescence tubulaire des reins, des infiltrations ponctuelles par des monocucléaires et l'aspect fibreux constituent les traces morphologiques qui demeurent après une attaque de leptospirose. Le pourcentage élevé (48,6) de ces lésions trouvées dans les les reins des rats examinés semble être plus en rapport avec le sérotypeicterohaemorrhagiae qu'avec le sérotypepomona. L'importance du sérotypeicterohaemorrhagiae chez le porc de la Cauca Valley est encore à vérifier.