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C Fergani JE Routly DN Jones LC Pickavance RF Smith H Dobson 《Reproduction in domestic animals》2014,49(3):433-440
Normal reproductive function is dependent upon availability of glucose and insulin‐induced hypoglycaemia is a metabolic stressor known to disrupt the ovine oestrous cycle. We have recently shown that IIH has the ability to delay the LH surge of intact ewes. In the present study, we examined brain tissue to determine: (i) which hypothalamic regions are activated with respect to IIH and (ii) the effect of IIH on kisspeptin cell activation and CRFR type 2 immunoreactivity, all of which may be involved in disruptive mechanisms. Follicular phases were synchronized with progesterone vaginal pessaries and at 28 h after progesterone withdrawal (PW), animals received saline (n = 6) or insulin (4 IU/kg; n = 5) and were subsequently killed at 31 h after PW (i.e., 3 h after insulin administration). Peripheral hormone concentrations were evaluated, and hypothalamic sections were immunostained for either kisspeptin and c‐Fos (a marker of neuronal activation) or CRFR type 2. Within 3 h of treatment, cortisol concentrations had increased whereas plasma oestradiol concentrations decreased in peripheral plasma (p < 0.05 for both). In the arcuate nucleus (ARC), insulin‐treated ewes had an increased expression of c‐Fos. Furthermore, the percentage of kisspeptin cells co‐expressing c‐Fos increased in the ARC (from 11 to 51%; p < 0.05), but there was no change in the medial pre‐optic area (mPOA; 14 vs 19%). CRFR type 2 expression in the lower part of the ARC and the median eminence was not altered by insulin treatment. Thus, disruption of the LH surge after IIH in the follicular phase is not associated with decreased kisspeptin cell activation or an increase in CRFR type 2 in the ARC but may involve other cell types located in the ARC nucleus which are activated in response to IIH. 相似文献
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Temuulen Ts. Sankey Jackson M. Leonard Margaret M. Moore 《Strength and Conditioning Journal》2019,72(5):858-863
Rangelands comprise a large component of the terrestrial land surface and provide critical ecosystem services, but they are degrading rapidly. Long-term rangeland monitoring with detailed, nonsubjective, quantitative observations can be expensive and difficult to maintain over time. Unmanned aerial vehicles (UAVs) provide an alternative means to gather unbiased and consistent datasets with similar details to field-based monitoring data. Comparing summer 2017 UAV images with long-term plot measurements, we demonstrate that rangeland vegetation cover changes can be accurately quantified and estimate an increase in total absolute shrub/subshrub cover from 34% in 1935 to > 80% in 2017 in central Arizona. We recommend UAV image-based rangeland monitoring for land managers interested in a few specific and dominant species, such as the foundation species, indicator species, or invasive species that require targeted monitoring. Land managers can identify and continuously monitor trends in rangeland condition, health, and degradation related to specific land use policies and management strategies. 相似文献
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Within the overall hypothesis that the periparturient relaxation of immunity (PPRI) to gastrointestinal parasites has a nutritional basis, we studied whether an increased intake of metabolizable protein (MP) could affect the expression of immunity to Teladorsagia (Ostertagia) circumcincta of twin-bearing and -rearing Greyface ewes. Two sets of iso-energetic diets were formulated to provide either 85% (L-) or 130% (H-) of the MP requirements during the last 6 weeks of pregnancy (-P) and the first 6 weeks of lactation (-L). Thirty ewes were allocated to LP or HP. Six ewes of each group were slaughtered after lambing (S(0)); the remaining 24 ewes of each group were allocated to either LL or HL, thus creating four treatment combinations during lactation (LP/LL, LP/HL, HP/LL and HP/HL; n=12). Six ewes of each treatment combination were slaughtered at Week 3 (S(3)) or at Week 6 (S(6)) in lactation.The HP ewes were heavier at lambing, produced heavier lambs, had lower faecal egg counts (FECs), a lower concentration of plasma pepsinogen, a higher concentration of plasma albumin during late pregnancy and more abomasal globule leukocytes (GLs) but similar worm burdens at S(0) compared to the LP ewes. The LP/HL and HP/HL ewes had a higher calculated milk production than the LP/LL and HP/LL ewes. FECs increased for each treatment combination during the first 3 weeks of lactation. This increase concurred with fewer abomasal GLs at S(3) compared to S(0). The LP/HL and HP/HL ewes had a higher proportion of immature worms and more abomasal GLs than the LP/LL and HP/LL ewes at S(6). The FECs continued to increase during the second 3 weeks of lactation for the LP/LL ewes only, which concurred with an elevated plasma pepsinogen concentration and an absence of abomasal GLs at S(6). The plasma pepsinogen concentration decreased rapidly for the LP/HL ewes during lactation. We conclude that an increased intake of MP could enhance the expression of immunity to T. circumcincta of twin-bearing and -rearing Greyface ewes. 相似文献
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Summary: The degree to which dog owners complied with instructions to administer a 5 to 10 day course of antimicrobial medication to their pets was assessed using microprocessor based monitoring devices. Twenty two clients gave an average of 84% of prescribed doses of amoxycillin-clavulanate. No difference was found between twice and thrice daily dosing regimens in the overall percentage of prescribed doses given. However, timing of doses was far from ideal in many cases and only 34% of doses were given within the designated optimum time period. Adherence to desired dosing intervals tended to be better with twice daily than with thrice daily dosing, although the difference was statistically insignificant. 相似文献
28.
Stevenson MA Morris RS Lawson AB Wilesmith JW Ryan JB Jackson R 《Preventive veterinary medicine》2005,69(1-2):129-144
In this paper we investigate area-level risk factors for BSE for the cattle population present in Great Britain between 1986 and 1997. By dividing this population into two birth cohorts, those born before the July 1988 ban on feeding ruminant-derived meat and bone meal to ruminants and those born after, second-order regional influences are distinguished from the strong first-order south-to-north gradient of area-level BSE risk using Bayesian hierarchical models that account for structured (spatially correlated) and unstructured heterogeneity in the data. For both cohorts area-level risk of BSE was increased by a more southerly location and greater numbers of dairy cattle, relative to non-dairy cattle. For the cohort of cattle born after the July 1988 ban on feeding ruminant-derived meat and bone meal area-level BSE risk was additionally associated with greater numbers of pigs, relative to cattle. These findings support the role of low level cross-contamination of cattle feed by pig feed as an influence on BSE incidence risk as the epidemic evolved. Prior to the 1988 meat and bone meal ban unexplained BSE risk was relatively uniformly distributed across the country whereas after the ban there were spatially aggregated areas of unexplained risk in the northern and eastern regions of England suggesting that local influences allowed BSE control measures to be less-successfully applied in these areas, compared with the rest of the country. We conclude that spatially localised influences were operating in divergent ways during the two phases of the epidemic. 相似文献
29.
Testosterone concentrations in stallions showed a seasonal trend with peak concentrations in the spring (April and May in Britain) and lowest concentrations in the period from December to February. The effect on this pattern of changing the length of the photoperiod at the end of the normal breeding season (mid-summer's day) was studied in 2 experiments. In the first experiment artificial illumination was organised from 21 June to mimic the effect of transfer to a southern hemisphere spring and summer, that is short days becoming longer. The stallions had low concentrations of testosterone in February and high concentrations in April. Concentrations in July, August and September were extremely low with a return to high values in late November/early December. In the second experiment, illumination was maintained at the equivalent of a 16 1/2-hour day from 21 June to mid December. These stallions had high testosterone concentrations in April, after which they fell until August, later rising to a maximum in October. These results are discussed in relation to transfer of stallions between the northern and southern hemispheres. 相似文献
30.
Paul Miguet Heather B. Jackson Nathan D. Jackson Amanda E. Martin Lenore Fahrig 《Landscape Ecology》2016,31(6):1177-1194