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51.
Georg Lehner Monika Linek Ross Bond David H. Lloyd Ellen Prenger-Berninghoff Nina Thom Iris Straube Kristien Verheyen Anette Loeffler 《Veterinary microbiology》2014
Methicillin-resistant Staphylococcus pseudintermedius (MRSP) has emerged as a highly drug-resistant small animal veterinary pathogen. Although often isolated from outpatients in veterinary clinics, there is concern that MRSP follows a veterinary-hospital-associated epidemiology. This study's objective was to identify risk factors for MRSP infections in dogs and cats in Germany. Clinical isolates of MRSP cases (n = 150) and methicillin-susceptible S. pseudintermedius (MSSP) controls (n = 133) and their corresponding host signalment and medical data covering the six months prior to staphylococcal isolation were analysed by multivariable logistic regression. The identity of all MRSP isolates was confirmed through demonstration of S. intermedius-group specific nuc and mecA. In the final model, cats (compared to dogs, OR 18.5, 95% CI 1.8–188.0, P = 0.01), animals that had been hospitalised (OR 104.4, 95% CI 21.3–511.6, P < 0.001), or visited veterinary clinics more frequently (>10 visits OR 7.3, 95% CI 1.0–52.6, P = 0.049) and those that had received topical ear medication (OR 5.1, 95% CI 1.8–14.9, P = 0.003) or glucocorticoids (OR 22.5, 95% CI 7.0–72.6, P < 0.001) were at higher risk of MRSP infection, whereas S. pseudintermedius isolates from ears were more likely to belong to the MSSP-group (OR 0.09, 95% CI 0.03–0.34, P < 0.001). These results indicate an association of MRSP infection with veterinary clinic/hospital settings and possibly with chronic skin disease. There was an unexpected lack of association between MRSP and antimicrobial therapy; this requires further investigation but may indicate that MRSP is well adapted to canine skin with little need for selective pressure. 相似文献
52.
Growth of cultured mussels (Perna canaliculus Gmelin 1791) at a deep-water chlorophyll maximum layer
Shaun C Ogilvie Steve P Fox Alex H Ross Mark R James David R Schiel 《Aquaculture Research》2004,35(13):1253-1260
We tested the hypothesis that yield of the mussel Perna canaliculus (Gmelin 1791) could be increased by placing culture ropes deep into the water column to take advantage of deep‐water chlorophyll a maxima. The study site, in Pelorus Sound, New Zealand, showed periods of thermal stratification of the water column, causing a high concentration of phytoplankton in deeper water, at the thermocline. Mussels were grown simultaneously at 5 and 17 m, for up to 96 days. Mussel growth and condition index data showed no significant differences between the two depth treatments, indicating that in this system there may not be substantial mussel productivity benefits from lowering mussel farms to the deep‐water chlorophyll maximum layer. 相似文献
53.
1. Development of effective conservation strategies for freshwater biodiversity must take account of the trade‐off between species preservation and human use of ecosystem goods and services. The latter cannot be prevented, and attempts to manage ecosystems that focus solely on maximizing biodiversity will fail. 2. A compromise position of management for ecosystem functioning and human livelihoods — rather than preservation of every species — will provide a better basis for biodiversity conservation. This has implications for the management of exotic species. 3. There are some situations, in lentic habitats in Sri Lanka, for example, where the establishment of exotics has increased fishery yields without apparent detriment to native biodiversity. The Sepik River in Papua New Guinea provides another illustration of potential compromises between human livelihoods and biodiversity conservation. 4. The Sepik supports a relatively unproductive fishery. Two fish stocking projects (in 1987–93 and 1993–97), representing a partnership between the Papua New Guinea Government and the United Nations (UNDP/FAO), led to the introduction of a suite of exotic fish into the Sepik. Species were selected on the basis of their potential to occupy niches not filled by native fish. Unfortunately, the outcomes of these introductions are poorly documented, although there is preliminary evidence both of increased human use of exotic fish as well as declines of some native species associated with the spread of exotics. 5. Better understanding of the results of the Sepik fish introductions is important, because the pressures of burgeoning human populations in most of tropical Asia make it impossible to preserve near‐pristine environments such as the Sepik. While attempts to conserve natural or near‐natural systems must remain a priority, there is a need to develop strategies for the management of damaged or degraded ecosystems, which may contain exotics, with the aim of maintaining ecosystem functioning and, if possible, maximizing the persistence of native biodiversity. Copyright © 2006 John Wiley & Sons, Ltd. 相似文献
54.
RL Westemeier JD Brawn SA Simpson TL Esker RW Jansen JW Walk EL Kershner JL Bouzat KN Paige 《Science (New York, N.Y.)》1998,282(5394):1695-1698
Effects of small population size and reduced genetic variation on the viability of wild animal populations remain controversial. During a 35-year study of a remnant population of greater prairie chickens, population size decreased from 2000 individuals in 1962 to fewer than 50 by 1994. Concurrently, both fitness, as measured by fertility and hatching rates of eggs, and genetic diversity declined significantly. Conservation measures initiated in 1992 with translocations of birds from large, genetically diverse populations restored egg viability. Thus, sufficient genetic resources appear to be critical for maintaining populations of greater prairie chickens. 相似文献
55.
Growth, feed conversion and survival were determined for juvenile Macrobrachium rosenbergii held in tanks under semi-controlled environmental conditions. Feeding trials incorporated water-stable diets at three levels of protein (15, 25 and 35%). The principal protein source combinations consisted either of soybean and tuna meal, or of soybean, tuna and shrimp meal. In a 244-day comparison of these diets, higher protein content produced larger prawns (P < 0.01), but differences between sources were not significant. No significant differences existed between feed conversion ratios (range 1.36–1.72) or percentage survival (range 90.3–93.6%). Trials of several other diets were also conducted, including soybean and Tilapia meal, and copra and Tilapia meal (25% protein level) as principal protein source combinations. After 167 days on these diets, growth was inferior to that obtained with soybean and tuna meal or soybean, tuna and shrimp meal combinations. No significant differences existed between feed conversion ratios or percentages of survival.For the 244 days, a control group of prawns received no formulated diet. Growth and survival in this group during the first 110 days suggested that naturally occurring algae contributed substantially to the prawns' nutrition.Mean prawn length after 244 days on the best diet (35% protein from soybean and tuna meal) was 73 mm, and growth rate was equivalent to that achieved under pilot pond conditions. 相似文献
56.
Blagburn BL Dryden MW Payne P Rust MK Jacobs DE Bond R Hutchinson MJ Denholm I Mehlhorn H Vaughn M Mencke N Schroeder I Hostetler J Endrizzi M 《Veterinary therapeutics : research in applied veterinary medicine》2006,7(2):86-98
A flea larval bioassay was developed by an international team of scientists to monitor the susceptibility of fleas (Ctenocephalides felis) to imidacloprid (Advantage, Bayer HealthCare). The assay was validated using laboratory and field isolates of C. felis. Flea eggs representing different field isolates of C. felis were collected by veterinarians in the United States, United Kingdom, and Germany. Of the 972 flea isolates obtained during the 5-year study, 768 contained sufficient numbers of eggs to conduct the larval bioassay. Greater than 5% survival occurred for only six of the field isolates evaluated. Further evaluation and analysis of these isolates demonstrated that they did not differ significantly in their susceptibility to imidacloprid from the reference strains used to develop the assay. Collections of field flea isolates will continue in an attempt to detect and document any change in the susceptibility of field flea populations to imidacloprid. 相似文献
57.
Soil carbon dioxide (CO2) flux is an integrative measure of ecosystem functioning representing both biotic and physical controls over carbon (C) balance. In the McMurdo Dry Valleys of Antarctica, soil CO2 fluxes (approximately −0.1-0.15 μmol m−2 s−1) are generally low, and negative fluxes (uptake of CO2) are sometimes observed. A combination of biological respiration and physical mechanisms, driven by temperature and mediated by soil moisture and mineralogy, determine CO2 flux and, therefore, soil organic C balance. The physical factors important to CO2 flux are being altered with climate variability in many ecosystems including arid forms such as the Antarctic terrestrial ecosystems, making it critical to understand how climate factors interact with biotic drivers to control soil CO2 fluxes and C balances. We measured soil CO2 flux in experimental field manipulations, microcosm incubations and across natural environmental gradients of soil moisture to estimate biotic soil respiration and abiotic sources of CO2 flux in soils over a range of physical and biotic conditions. We determined that temperature fluctuations were the most important factor influencing diel variation in CO2 flux. Variation within these diel CO2 cycles was explained by differences in soil moisture. Increased temperature (as opposed to temperature fluctuations) had little or no effect on CO2 flux if moisture was not also increased. We conclude that CO2 flux in dry valley soils is driven primarily by physical factors such as soil temperature and moisture, indicating that future climate change may alter the dry valley soil C cycle. Negative CO2 fluxes in arid soils have recently been identified as potential net C sinks. We demonstrate the potential for arid polar soils to take up CO2, driven largely by abiotic factors associated with climate change. The low levels of CO2 absorption into soils we observed may not constitute a significant sink of atmospheric CO2, but will influence the interpretation of CO2 flux for the dry valley soil C cycle and possibly other arid environments where biotic controls over C cycling are secondary to physical drivers. 相似文献
58.
125I-labelling was used to characterise the surface components of five stocks of Trypanosoma evansi. Two components of 67 and 60.5 kD were labelled in two of the stocks, a single 60.5 kD component in two other stocks and no components in the remaining stock. These differences are probably related to the labelling method and biochemical differences between the stocks. 相似文献
59.
D.J. Ross K.R. Tate Annette Cairns Elizabeth A. Pansier 《Soil biology & biochemistry》1980,12(4):375-383
Microbial biomass was determined by three biochemical procedures in nine topsoils from a climosequence in tussock grasslands. The pH values of the samples ranged from 4.4 to 6.2 and organic C contents from 2.5 to 20.0%. When determined by a chloroform-fumigation procedure, contents of biomass C and mineral-N (Min-N) flush ranged from 530–2780 and 59–167 μgg?1 dry soil respectively. Adenosine 5'-triphosphate (ATP) content ranged from 2.2 to 10.7 μg g?1 dry soil. All three estimates were significantly correlated with each other and with several soil properties, including organic C and total N contents and CO2 production. They were not significantly correlated with any climatic factor.In spite of these significant correlations, the ratios of the biomass estimates varied appreciably in the different soils. The ratios of biomass C/Min-N flush ranged from 7.8 to 22.8 (average 12.5), biomass C/ATP from 163 to 423 (average 248) and Min-N flush/ATP from 12 to 35 (average 22). These ratios were mostly higher than those found elsewhere for Australian and English soils. The high biomass C/ATP and Min-N flush/ATP ratios did not appear to originate from inefficient extraction of “native” ATP or from the soils' P status. Based on these results, care in the use of factors for obtaining soil microbial biomass content from Min-N flush or ATP values is indicated. 相似文献