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61.
Production of an epidermal growth factor receptor-related protein 总被引:24,自引:0,他引:24
Human epidermoid carcinoma A431 cells in culture produce a soluble 105-kilodalton protein which, by the criteria of epidermal growth factor (EGF) binding, recognition by monoclonal and polyclonal antibodies to the EGF receptor, amino-terminal sequence analysis and carbohydrate content, is related to the cell surface domain of the EGF receptor. The high rate of production and the finding that with biosynthetic labeling the specific activity of this 105-kilodalton protein exceeds that of the intact receptor indicate that it is not derived from membrane-bound mature receptor but is separately produced by the cell. These cells thus separately synthesize an EGF receptor that is inserted into the membrane and an EGF receptor-related protein that is secreted. 相似文献
62.
63.
Spatial Variability of Turbulent Mixing in the Abyssal Ocean 总被引:3,自引:0,他引:3
Ocean microstructure data show that turbulent mixing in the deep Brazil Basin of the South Atlantic Ocean is weak at all depths above smooth abyssal plains and the South American Continental Rise. The diapycnal diffusivity there was estimated to be less than or approximately equal to 0.1 x 10(-4) meters squared per second. In contrast, mixing rates are large throughout the water column above the rough Mid-Atlantic Ridge, and the diffusivity deduced for the bottom-most 150 meters exceeds 5 x 10(-4) meters squared per second. Such patterns in vertical mixing imply that abyssal circulations have complex spatial structures that are linked to the underlying bathymetry. 相似文献
64.
Gill HA 《Science (New York, N.Y.)》1917,45(1165):416
65.
66.
A radiotracer method for the determination of mercury (Hg) methylation rates in bulk water and water overlying intact sediment cores has been developed. A sediment core with overlying water is collected in a core tube, the overlying water is spiked with high specific activity 203-Hg radiotracer, and the core is incubated at ambient temperature. Aliquots of the overlying water are removed, the Hg is extracted from the sample, and the activity in the extract is measured. A 10–25 fold sample preconcentration is achieved using a dithizone-chloroform extraction technique and a sodium nitrite back extraction step to separate inorganic Hg(II) from monomethylmercury (MMHg). The use of this technique, in conjunction with high specific activity 203-Hg, has allowed for spiking concentrations in the overlying water of approximately 1 ng Hg/L. This spiking level is about the same concentration as the ambient water overlying the core, thus not significantly perturbing the system. Our technique is a significant improvement over previous methodologies which used 203-Hg spike additions of 1 μg Hg/L or higher. The technique was used to measure Hg methylation rates at the Experimental Lakes Area (ELA) in Ontario, Canada during August of 1993 and at an extensively studied estuarine site in Gulf Breeze, Florida, USA during September, 1993 and June, 1994. Multiple cores were collected and spiked with a range of 1 to 11,800 ng Hg (as 203-Hg) into the overlying water. MMHg production at the ELA site indicated rates of 0.25 to 3.7 pg/cm2/day (0.08 to 2.5 % methylation/day). Results from Gulf Breeze were significantly higher at 1.5 to 425 pg/cm2/day or 0.06 to 18 % methylation/day. These rates are one to three orders of magnitude greater than previously measured “specific rates” in bulk water samples and sediments. A direct comparison of rates with previous sediment methylation assay techniques is not possible, however, because of the significant differences between our methodology and previous assay protocols. 相似文献
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68.
Enzyme activities and microbial community structure in semiarid agricultural soils 总被引:15,自引:0,他引:15
Acosta-Martínez V. Zobeck T. M. Gill T. E. Kennedy A. C. 《Biology and Fertility of Soils》2003,38(4):216-227
This study investigated the effect of management on -glucosidase, -glucosaminidase, alkaline phosphatase, and arylsulfatase activities and the microbial community structure in semiarid soils from West Texas, USA. Surface samples (0–5 cm) were taken from a fine sandy loam, sandy clay loam, and loam that were under continuous cotton ( Gossypium hirsutum L.) or in cotton rotated with peanut ( Arachis hypogaea L.), sorghum ( Sorghum bicolor L.), rye ( Secale cereale) or wheat ( Triticum aestivum L.), and had different water management (irrigated or dryland), and tillage (conservation or conventional). The enzyme activities were higher in the loam and sandy clay loam than in the fine sandy loam. Soil pH was not affected by management, but the soil organic C and total N contents were generally affected by the different crop rotations and tillage practices studied. The trends of the enzyme activities as affected by management depended on the soil, but in general crop rotations and conservation tillage increased the enzyme activities in comparison to continuous cotton and conventional tillage. The soil enzyme activities were significantly correlated with the soil organic C ( r -values up to 0.90, P< 0.001), and were correlated among each other ( r -values up to 0.90, P <0.001). There were differences in the fatty acid methyl ester profiles between the fine sandy loam and the sandy clay loam and loam, and they reflected the differences in the enzyme activities found among the soils. For example, a 15:0 ranged from 1.61±0.25% in cotton-peanut/irrigated/no-till in the fine sandy loam to 3.86±0.48% in cotton-sorghum/dryland/conservation tillage in the sandy clay loam. There were no differences due to management within the same soil.Trade names and company names are included for the benefit of the reader and do not infer any endorsement or preferential treatment of the product by USDA-ARS 相似文献
69.
M.V.K. Sivakumar N. Seetharama K.S. Gill R.C. Sachan 《Agricultural Water Management》1981,3(4):279-289
The response of sorghum (Sorghum bicolor L.) to moisture stress during the post-rainy season was studied at ICRISAT research center on a medium deep Alfisol using a line source sprinkler irrigation system. Changes in soil moisture content, stomatal conductance, leaf-water potential and leaf temperature of sorghum as a function of distance away from the line source sprinkler system were monitored throughout the season. Use of the line source technique facilitated the imposition of a range of moisture stress levels as indicated by increased water use by sorghum closer to the line source compared with the crop farther away from the line source. Canopy response measured in terms of stomatal conductance, leaf-water potential, and leaf temperature clearly reflected the gradient in moisture stress perpendicular to the line source. 相似文献
70.
Gill JM 《New Zealand veterinary journal》1994,42(2):77
Abstract Thirty percent of 60 goldfish in an outside pond showed progressive development of swollen abdomens and floatation problems. The occasional fish was found dead. Necropsy of an affected fish showed an abdomen almost filled with a large bilobed ventral mass comprised of masses of cysts filled with clear fluid. On histopathological examination, this mass appeared to be composed of large cystic spaces lined by a single flattened epithelium. Occasional remnants of normal kidney tissue were seen withen the mass. 相似文献