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31.
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Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs 总被引:5,自引:0,他引:5
Erika Varkonyi-Gasic Rongmei Wu Marion Wood Eric F Walton Roger P Hellens 《Plant methods》2007,3(1):12
MicroRNAs (miRNAs) are a class of small non-coding RNAs with a critical role in development and environmental responses. Efficient
and reliable detection of miRNAs is an essential step towards understanding their roles in specific cells and tissues. However,
gel-based assays currently used to detect miRNAs are very limited in terms of throughput, sensitivity and specificity. Here
we provide protocols for detection and quantification of miRNAs by RT-PCR. We describe an end-point and real-time looped RT-PCR
procedure and demonstrate detection of miRNAs from as little as 20 pg of plant tissue total RNA and from total RNA isolated
from as little as 0.1 μl of phloem sap. In addition, we have developed an alternative real-time PCR assay that can further
improve specificity when detecting low abundant miRNAs. Using this assay, we have demonstrated that miRNAs are differentially
expressed in the phloem sap and the surrounding vascular tissue. This method enables fast, sensitive and specific miRNA expression
profiling and is suitable for facilitation of high-throughput detection and quantification of miRNA expression. 相似文献
33.
The effect of streptomycin, oxytetracycline, tilmicosin and phenylbutazone on spermatogenesis in bulls
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Barth AD Wood MR 《The Canadian veterinary journal. La revue veterinaire canadienne》1998,39(2):103-106
To determine whether declining semen quality associated with health problems may be due to certain antibiotic or anti-inflammatory treatments, semen was collected 3 times per week for up to 42 d from 6 normal bulls after treatment with oxytetracycline, tilmicosin, dihydrostreptomycin, or phenylbutazone. No adverse effects on semen quality were observed. 相似文献
34.
Barbara I. Zemann Antony S. Moore William M. Rand Gail Mason David M. Ruslander Angela E. Frimberger Carrie A. Wood Deborah A. L'Heureux John Gliatto Susan M. Cotter 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1998,12(6):465-470
Ninety-eight dogs with lymphoma treated with a 5-drug combination chemotherapy regimen (vincristine, L-asparaginase. cyclophosphamide, doxorubicin, prednisone [VELCAP-L]) were evaluated for pretreatment characteristics predictive for response and remission duration. The complete remission rate was 69%, with a median remission duration of 55 weeks. Dogs with advanced stage of disease, constitutional signs, dogs that were older, and dogs that were dyspneic were less likely to achieve remission. Once in remission, small dogs and dogs without pretreatment thrombocytopenia were likely to have longer remission duration. Toxicoses were frequent, but rarely fatal, and no predictitive factors were found for a dog developing toxicoses. VELCAP-L is an effective treatment for dogs in stage I-III lymphoma, particularly in young, small animals. 相似文献
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Wood J 《Science (New York, N.Y.)》1893,21(522):62-64
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XU Ming REN Haiqing LI Xiazhen Research Institute of Wood Industry Chinese Academy of Forestry Beijing P.R.China 《中国林业科技(英文版)》2007,(3)
The variation of tensile strength parallel to grain in Chinese fir and I-214 poplar wood from plantations was studied in this paper. Aaccording to the national standards The Testing Methods for Physical and Mechanical Properties of Wood (GB 1927 to 1943 ? 1991), the small clear wood samples were cut and tested, which represent the south and north of trees with heights of 1.3, 3.3, 5.3 and 7.3 m. The results showed: the tensile strength parallel to grain of the north wood was higher than that of south wood. ANOVA shows that the tensile strength parallel to the grain with height is of significant difference (at 0.05 level). 相似文献