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51.
Ruminant animals and ruminal microorganisms have a symbiotic relationship that facilitates fiber digestion, but domestic ruminants in developed countries are often fed an abundance of grain and little fiber. When ruminants are fed fiber-deficient rations, physiological mechanisms of homeostasis are disrupted, ruminal pH declines, microbial ecology is altered, and the animal becomes more susceptible to metabolic disorders and, in some cases, infectious disease. Some disorders can be counteracted by feed additives (for example, antibiotics and buffers), but these additives can alter the composition of the ruminal ecosystem even further. 相似文献
52.
How genes with newly characterized functions originate remains a fundamental question. PMCHL1 and PMCHL2, two chimeric genes derived from the melanin-concentrating hormone (MCH) gene, offer an opportunity to examine such an issue in the human lineage. Detailed structural, expression, and phylogenetic analysis showed that the PMCHL1 gene was created near 25 million years ago (Ma) by a complex mechanism of exon shuffling through retrotransposition of an antisense MCH messenger RNA coupled to de novo creation of splice sites. PMCHL2 arose 5 to 10 Ma by an event of duplication involving a large chromosomal region encompassing the PMCHL1 locus. The RNA expression patterns of those chimeric genes suggest that they have been submitted to strong regulatory constraints during primate evolution. 相似文献
53.
The development of resistance is the main threat to the long-term use of toxins from Bacillus thuringiensis (Bt) in transgenic plants. Here we report the cloning of a Bt toxin resistance gene, Caenorhabditis elegans bre-5, which encodes a putative beta-1,3-galactosyltransferase. Lack of bre-5 in the intestine led to resistance to the Bt toxin Cry5B. Wild-type but not bre-5 mutant animals were found to uptake toxin into their gut cells, consistent with bre-5 mutants lacking toxin-binding sites on their apical gut. bre-5 mutants displayed resistance to Cry14A, a Bt toxin lethal to both nematodes and insects; this indicates that resistance by loss of carbohydrate modification is relevant to multiple Bt toxins. 相似文献
54.
Frye MA Johnson JS Traub-Dargatz JL Savage CJ Fettman MJ Gould DH 《Journal of the American Veterinary Medical Association》2001,218(4):560-566
OBJECTIVE: To determine historical, physical examination, clinicopathologic, and postmortem findings in horses with putative uremic encephalopathy. Design-Retrospective study. Animals-5 horses with renal failure and neurologic disease not attributable to abnormalities in any other organ system. PROCEDURE: Medical records from 1978 to 1998 were examined for horses with renal disease and neurologic signs not attributable to primary neurologic, hepatic, or other diseases. Signalment, history, physical examination findings, clinicopathologic data, renal ultrasonographic findings, and postmortem data were reviewed. RESULTS: Of 332 horses with renal disease, 5 met selection criteria. Historical findings, physical examination findings, clinicopathologic data, ultrasonographic data, and postmortem findings were consistent with chronic renal failure. Swollen astrocytes were detected in all 4 horses examined at necropsy. CONCLUSIONS AND CLINICAL RELEVANCE: A single criterion was not determined to be pathognomonic for uremic encephalopathy in horses. Uremic encephalopathy should be considered as a differential diagnosis in horses with evidence of chronic renal failure and encephalopathic neurologic sign not attributable to other causes. Astrocyte swelling, which was common to all 4 horses examined at necropsy, may serve as a microscopic indicator of uremic encephalopathy in horses. 相似文献
55.
OBJECTIVE: To test the sensitivity to measurement and modeling errors of a method for noninvasive calculation of flexor tendon forces in the equine forelimb and to calculate tendon forces for Dutch Warmblood horses during trotting. SAMPLE POPULATION: A normative set of kinematic and ground-reaction force (GRF) data obtained from horses during trotting in another study. PROCEDURE: Forces in the flexor tendons were calculated from the data set before and after addition of fixed relative and absolute errors. Amount of error was based on normal accuracy of the variables. A similar analysis was performed for a measure of strain of the accessory ligament of the deep digital flexor tendon. RESULTS: The only errors that had a substantial influence on accuracy were modeling errors in the mechanical properties of the suspensory ligament and measurement errors in the point of application of the GRF and position of the marker on the distal interphalangeal joint. Influence of the measurement errors could be minimized by applying usual correction methods. CONCLUSIONS AND CLINICAL RELEVANCE: After correction of measurement errors, the method can be used to calculate mean tendon forces for a group of horses and to evaluate the influence of factors such as surface properties, type of shoe, speed, and fatigue on tendon forces. The method could become an important tool for use in research on the cause, prevention, and treatment of tendon injuries in horses. 相似文献
56.
Howe LM Slater MR Boothe HW Hobson HP Holcom JL Spann AC 《Journal of the American Veterinary Medical Association》2001,218(2):217-221
OBJECTIVE: To determine long-term results and complications of gonadectomy performed at an early age (prepubertal) or at the traditional age in dogs. DESIGN: Cohort study. ANIMALS: 269 dogs from animal shelters. PROCEDURE: Dogs that underwent gonadectomy were allotted to 2 groups on the basis of estimated age at surgery (traditional age, > or =24 weeks old; prepubertal, < 24 weeks old). Adoptive owner information was obtained from shelter records, and telephone interviews were conducted with owners to determine physical or behavioral problems observed in the dogs since adoption. Follow-up information was obtained from attending veterinarians for dogs with complex problems or when owners were uncertain regarding the exact nature of their dog's problem. RESULTS: Prepubertal gonadectomy did not result in an increased incidence of behavioral problems or problems associated with any body system, compared with traditional-age gonadectomy, during a median follow-up period of 48 months after gonadectomy. Rate of retention in the original adoptive household was the same for dogs that underwent prepubertal gonadectomy as those that underwent traditional-age gonadectomy. Infectious diseases, however, were more common in dogs that underwent prepubertal gonadectomy. CONCLUSIONS AND CLINICAL IMPLICATIONS: With the exception of infectious diseases, prepubertal gonadectomy may be safely performed in dogs without concern for increased incidence of physical or behavioral problems during at least a 4-year period after gonadectomy. 相似文献
57.
Woodworth JC Tokach MD Goodband RD Nelssen JL O'Quinn PR Knabe DA Said NW 《Journal of animal science》2001,79(5):1280-1287
We conducted three experiments to determine the apparent ileal digestibility of amino acids (Exp. 1), metabolizable and digestible energy (Exp. 2), and feeding value (Exp. 3) of dry extruded-expelled soybean meal with (DEH) or without (DENH) hulls compared with solvent-extracted soybean meal with hulls removed (SBMNH). Soybeans used to produce DEH were unadulterated prior to extrusion, but those used for DENH were dehulled prior to extrusion. In Exp. 1, six nonlittermate barrows (initially 39 kg) were fitted with ileal T-cannulas and used in a replicated 3 x 3 Latin square design digestion trial. Experimental diets (0.80% total lysine) were cornstarch-based and contained soybean meal from one of the three different sources as the sole source of lysine. Apparent ileal digestibilies of nutrients were similar (P > 0.10) for DEH and DENH. Apparent ileal digestibilies of CP, Lys, Ile, Leu, Arg, Phe, and Val were greater (P < 0.05) for DEH and DENH than for SBMNH. In Exp. 2, six barrows (initially 41 kg) were fed three corn-based diets containing 25% of one of the three soybean meal sources. A fourth diet was fed at the end of the trial containing all ingredients except soybean meal, so that energy values of the soybean meal could be determined by difference. Digestible energy and ME contents were similar (P > 0.10) for DEH and DENH and both had greater (P < 0.05) DE and ME contents than SBMNH. In Exp. 3, pigs (n = 216, initially 10.6 +/- 1.3 kg and 35 +/- 3 d of age) were blocked by weight and allotted to six dietary treatments. Corn-soybean meal-based diets (0.95% digestible lysine and 3.44 kcal/g ME) containing DEH or DENH were compared with similar diets containing SBMNH or solvent-extracted soybean meal with hulls (SBMH). In addition, a diet containing a second expelled soybean meal with hulls (ESBM) was compared with a diet containing SBMH and soy oil. Growth performance of pigs fed diets containing DEH or DENH was not different (P > 0.10) than that of pigs fed corresponding diets containing SMBH or SBMNH. Pigs fed ESBM had lower (P < 0.05) ADG and G/F compared with its corresponding SBMH and soy oil diet. In conclusion, DEH and DENH are more digestible than conventional soybean meal and can be successfully used in swine diets. 相似文献
58.
The effect of exogenous iron and folate on reproductive performance in swine is equivocal. However, the effect of exogenous iron and folate on secretion of their respective uterine transport proteins has never been reported. Twenty gilts were infused (n = 5 per treatment) with either 1) saline, 2) alpha-tocopherol, 3) alpha-tocopherol plus iron citrate, or 4) alpha-tocopherol plus tetrahydrofolate on d 11 to 14 of pregnancy. Intravenous infusion of iron citrate and tetrahydrofolate increased (P < 0.05) plasma iron and folate, respectively, for 6 to 8 h after treatment. Treatments had no effect on uterine content of uteroferrin or secreted folate-binding protein in uterine flushings obtained on d 15 of pregnancy. These data suggest that uterine secretion of uteroferrin and secreted folate-binding protein are not influenced by plasma levels of iron and folate, respectively, and may provide an explanation for the equivocal effect of iron and folate treatment on reproductive performance in gilts. 相似文献
59.
60.
OBJECTIVE: To measure the effect of subject velocity on hind limb ground reaction force variables at the walk and to use the data to predict the force variables at different walking velocities in horses. ANIMALS: 5 clinically normal horses. PROCEDURE: Kinematic and force data were collected simultaneously. Each horse was led over a force plate at a range of walking velocities. Stance duration and force data were recorded for the right hind limb. To avoid the effect of horse size on the outcome variables, the 8 force variables were standardized to body mass and height at the shoulders. Velocity was standardized to height at the shoulders and expressed as velocity in dimensionless units (VDU). Stance duration was also expressed in dimensionless units (SDU). Simple regression analysis was performed, using stance duration and force variables as dependent variables and VDU as the independent variable. RESULTS: Fifty-six trials were recorded with velocities ranging from 0.24 to 0.45 VDU (0.90 to 1.72 m/s). Simple regression models between measured variables and VDU were significant (R2 > 0.69) for SDU, first peak of vertical force, dip between the 2 vertical force peaks, vertical impulse, and timing of second peak of vertical force. CONCLUSION AND CLINICAL RELEVANCE: Subject velocity affects vertical force components only. In the future, differences between the forces measured in lame horses and the expected forces calculated for the same velocity will be studied to determine whether the equations can be used as diagnostic criteria. 相似文献