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Objective To determine the prevalence of mastitis pathogens in high‐producing intensive dairy herds in New South Wales. Design Field survey. Procedure Milk samples from the mastitis‐affected quarter were collected from cows on five high‐producing dairy farms in NSW. The 820 samples were cultured using standard microbiological culture techniques. Results Bacteria or fungi were isolated from 83.3% of samples (683/820). More than two colony types were isolated from 16.7% of samples (137/820), two types from 6.6% (54/820), and one type from 52.3% (429/820). No bacteria were isolated from 24.4% (200/820) of the primary cultures, but enrichment cultures of these samples yielded single colony type bacterial isolates from 36.5% (73/200) of samples. Environmental pathogens, including coliforms, environmental Streptococcus and Staphylococcus spp., made up 91% (555/610) of isolates and accounted for 33.6% (205/610), 41.6% (254/610) and 15.7% (96/610), respectively, of isolates. Escherichia coli accounted for 76.1% (156/205) of the coliform isolates, Streptococcus uberis and Streptococcus dysgalactiae accounted for 32.3% (82/254) and 28.0% (71/254), respectively, of the environmental streptococcal isolates. Contagious pathogens were uncommon, comprising only 2.5% (15/610) of the total isolates. Conclusion The incidence and causes of mastitis are largely influenced by farm management. The relatively high prevalence of coliform mastitis in the intensive high‐producing herds in this survey contrasts with the low incidence reported in surveys of pasture‐based herds in Victoria. If the Australian dairy industry continues its current trend of intensification, coliform intra‐mammary infections may emerge as an increasingly important cause of mastitis. 相似文献
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JK Harmon SJ Ostro LAM Benner KD Rosema RF Jurgens R Winkler DK Yeomans D Choate R Cormier JD Giorgini DL Mitchell PW Chodas R Rose D Kelley MA Slade ML Thomas 《Science (New York, N.Y.)》1997,278(5345):1921-1924
Radar observations of comet Hyakutake (C/1996 B2) made at the Goldstone Deep Space Communications Complex in California have detected echoes from the nucleus and from large grains in the inner coma. The nucleus of this bright comet was estimated to be only 2 to 3 kilometers in diameter. Models of the coma echo indicate backscatter from porous, centimeter-size grains ejected anisotropically at velocities of tens of meters per second. The radar observations suggest that a comet's activity may be a poor indicator of its size and provide evidence that large grains constitute an important component of the mass loss from a typical active comet. 相似文献
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Design of a surface alloy catalyst for steam reforming 总被引:1,自引:0,他引:1
F Besenbacher I Chorkendorff BS Clausen B Hammer AM Molenbroek JK Norskov I Stensgaard 《Science (New York, N.Y.)》1998,279(5358):1913-1915
Detailed studies of elementary chemical processes on well-characterized single crystal surfaces have contributed substantially to the understanding of heterogeneous catalysis. Insight into the structure of surface alloys combined with an understanding of the relation between the surface composition and reactivity is shown to lead directly to new ideas for catalyst design. The feasibility of such an approach is illustrated by the synthesis, characterization, and tests of a high-surface area gold-nickel catalyst for steam reforming. 相似文献
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Estimating the mass of asteroid 253 mathilde from tracking data during the NEAR flyby 总被引:1,自引:0,他引:1
DK Yeomans J Barriot DW Dunham RW Farquhar JD Giorgini CE Helfrich AS Konopliv JV McAdams JK Miller WM Owen DJ Scheeres SP Synnott BG Williams 《Science (New York, N.Y.)》1997,278(5346):2106-2109
The terminal navigation of the Near Earth Asteroid Rendezvous (NEAR) spacecraft during its close flyby of asteroid 253 Mathilde involved coordinated efforts to determine the heliocentric orbits of the spacecraft and Mathilde and then to determine the relative trajectory of the spacecraft with respect to Mathilde. The gravitational perturbation of Mathilde on the passing spacecraft was apparent in the spacecraft tracking data. As a result of the accurate targeting achieved, these data could be used to determine Mathilde's mass as 1.033 (+/- 0.044) x 10(20) grams. Coupled with a volume estimate provided by the NEAR imaging team, this mass suggests a low bulk density for Mathilde of 1.3 grams per cubic centimeter. 相似文献
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Grassland vegetation changes and nocturnal global warming 总被引:4,自引:0,他引:4
Global minimum temperatures (TMIN) are increasing faster than maximum temperatures, but the ecological consequences of this are largely unexplored. Long-term data sets from the shortgrass steppe were used to identify correlations between TMIN and several vegetation variables. This ecosystem is potentially sensitive to increases in TMIN. Most notably, increased spring TMIN was correlated with decreased net primary production by the dominant C4 grass (Bouteloua gracilis) and with increased abundance and production by exotic and native C3 forbs. Reductions in B. gracilis may make this system more vulnerable to invasion by exotic species and less tolerant of drought and grazing. 相似文献
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Several outbreaks of anaemia, jaundice, abortion and mortality in cattle in New South Wales were attributed to the intracellular parasite, Theileria buffeli . Disease occurred predominantly in periparturient animals that had been moved from inland to coastal areas. Diagnosis was made via exclusion of other causes of haemolytic anaemia and observation of the parasite in blood smears. Treatments included both registered and non-registered products. There is a possibility of a new strain of Theileria sp. in Australia and the possible vectors encountered in NSW are discussed. 相似文献
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