连续热裂解法大规模提取质粒DNA

选择质粒保有率高及最适合发酵的TOP10菌株,在传统的煮沸裂解法提取质粒的基础上进行了提取方法的改进,使用恒流泵和热交换螺旋管,使菌体在流动过程中完成热裂解,释放质粒DNA。改进后的方法裂解温度可降到70",提取工艺流程简单,缩短了提取时间,提高了质粒DNA的提取效率。利用本方法提取的质粒大小正确、纯度合格,每升发酵液得率平均为100mg质粒DNA。     

北京山区泥石流与滑坡防治研究

泥石流、滑坡是北京山区常见的山地灾害 ,尤以泥石流灾害最为严重。全市共有泥石流沟 70 0条 ,分布在房山、门头沟、昌平、怀柔、延庆、密云、平谷 7个区县 ,涉及乡镇 6 1个。泥石流、滑坡多次造成人员伤亡和财产损失。防治泥石流、滑坡灾害 ,对改善北京山区环境 ,保护首都水源地等都有着重要意义。     

转基因羊研究进展

转基因动物已成为当今生命科学发展的热点，其中转基因羊的研究又是转基因动物中较为活跃的领域。自1985年用显微注射法获得了第1只转基因羊以来，随后有关转基因羊获得成功及取得突破的相关报道逐渐增多；同时转基因羊的研制目的也从当初的转基因育种逐渐倾向于乳腺生物反应器，并亦取得显著成效。本文就此作一简要综述。     

玉米抗粗缩病自交系种质的发掘和遗传多样性及其在育种中的应用

玉米粗缩病是近十年来危害我国部分玉米产区的主要病害之一, 挖掘抗粗缩病玉米种质, 进而培育抗病品种是防治粗缩病危害最经济有效的途径。选用184份常用玉米自交系(包括111份普通玉米和73份糯玉米)于2009—2010年在玉米粗缩病重发区江苏沿江地区农业科学研究所试验田进行田间自然鉴定; 同时利用117对SSR引物对供试的玉米自交系进行多态性检测, 按UPGMA方法, 利用Powermarker 3.0软件对184份自交系进行系统聚类分析。筛选出2份高抗(T877和YJ7)、6份抗病普通自交系和2份糯玉米抗性种质。基于644个SSR多态性位点, 结合系谱资料和育种实践, 将184份自交系划为9个杂种优势亚群。自交系4S及其衍生系(第VI亚群)和来源于美国杂交种78599的玉米自交系(第VIII亚群)为玉米抗粗缩病育种的重要种质。     

烤烟不同叶位叶片有机酸含量的差异分析

采用硫酸甲酯化前处理,结合气相色谱-质谱( GC/MS)分离分析技术,研究了烤烟不同叶位叶片(L8-L12,即从底部第8位叶向上到第12位叶)有机酸含量的差异.GC/MS定量分析结果表明:烤烟不同叶位叶片中,苹果酸、γ-戊酮酸、柠檬酸、丙二酸、乙二酸、亚麻酸和软脂酸含量相对较高,是烤烟中主要的有机酸成分;并且不同叶位叶...     

吉林省水稻品种对稻瘟病的抗性分析

选用22个稻瘟菌鉴别菌株,采用离体叶片接种法对吉林省全部水稻主栽品种及育种材料(98个)的抗瘟性进行了鉴定.结果显示,吉林省主栽水稻品种对稻瘟病的抗性较弱,高达98%的供试品种均可以被稻瘟菌不同程度地侵染;本研究筛选到3个品种:超级稻2号、吉粳801、吉粳806,具有极高的抗瘟性,为将来克隆新的抗瘟基因及培育新的抗瘟品...     

Are We Moving Back to the City? Examining Residential Mobility in the Washington,DC Metropolitan Area

This research assesses the extent to which there is evidence of population re‐centralization or back to the city moves by tracking the historical trend of household and income mobility in the Washington, DC metropolitan area. County‐to‐county migration data and four migration efficiency measures are used to investigate net flows of households and income in the region. The results show a nascent tendency of back to the city movement; however, the redistribution of households and income in the metropolitan area is more complex. While the region's core may be starting to gain households and income, there are still significant flows into the region's most distant suburbs. The results of this research have implications for transportation, housing, and economic development policy making in Washington, DC and other regions. The study also offers a unique example of how to study household and income redistribution within U.S. metropolitan areas.     

马铃薯新品种毕威薯1号的选育

为了解决马铃薯生产上的品种更新问题,采用六引519作母本,米拉作父本,经有性杂交育成马铃薯新品种毕威薯1号.该品种具有品质优良,丰产性、稳产性好,抗病性强,薯块经济性状好,商品薯率高等突出特点,于2010年7月通过贵州省农作物品种审定委员会审定.适宜在贵州省海拔770～2240m范围地区推广种植.     

Prediction of Rock Burst using the Method of Optimal Relative Membership Degree Based on the Maximum Entropy Principle

In the analysis of rock burst criterion prediction at home and abroad, the prediction standards of rock burst are selected including the conditions of mechanics、integrity、energy and brittle. The concept of relative membership degree on the rock burst prediction was introduced. The weight of standards and fuzzy matrix of relative membership degree are calculated. Uncertainty in rock burst prediction is described and compared according to the information entropy. Generalized weighted distance is also defined to characterize the differences in rock burst based on the maximum entropy principle, the establishment of a rock burst prediction fuzzy optimization model. The results from the application to practical example and comparisons with other methods are fairly good. Finally, the prediction model is applied in Putaoshan tunnel and the predictions are consistent with the actual rock burst.     

不同用量蜂蜜对水稻秧苗生长的影响

研究了不同用量蜂蜜条件下水稻秧苗的生长情况。结果表明：f1.0和f2.0用量处理在叶龄、叶绿素含量、活跃吸收面积、发根力、充实度等方面均优于ck和其它用量处理，说明了蜂蜜中的一些营养物质对秧苗生长有一定的促进作用，并增强了秧苗期抗低温的能力。