猪败血性链球菌与附红细胞体混合感染病例

猪败血性链球菌病是猪链球菌感染引起的一种急性败血性传染病,猪附红细胞体病是由猪附红细胞体寄生于猪红细胞而引起的一种散在的热性、溶血性传染病.本文以某猪场这2种病混合感染病例为题材,从发病基本情况、临床症状、剖检变化、实验室检验、防治措施等6个方面对该混合感染病例做了详尽的阐述.     

HPLC法测定盐酸氨丙啉的含量及对杂质2-甲基吡啶进行检查

采用高效液相色谱法测定盐酸氨丙啉的含量及对杂质2-甲基吡啶进行检查.用Waters XTerraTM RP C18色谱柱,以乙腈-甲醇-庚烷磺酸钠溶液(将12 g的1-庚烷磺酸钠溶于1000 mL水中,加24mL冰醋酸,6 mL的三乙胺制得)(5:35:60)为流动相,用紫外检测器于254nm处检测,柱温30℃,流速1.0 mL/min.在该条件下盐酸氨丙啉和2-甲基吡啶的分离度很好,盐酸氨丙啉浓度在0.05～0.50 mg/mL范围内,其色谱峰面积与浓度呈良好的线性关系,回归方程为y=3.07×107x-3.90×104,r=0.999 9,RSD=0.76%.该法适用于盐酸氨丙啉原料药的质量控制.     

Effect of L-carnitine on apoptosis and oxidant damage of cardiomyocytes induced by hypoxia/reoxygenation in vitro

AIM: To examine the effects of L-carnitine on apoptosis and oxidant injury in cultured neonatal rat cardiomyocytes induced by hypoxia/reoxygenation and its possible mechanism. METHODS: The cultured cardiomyocytes were divided into three groups， control， A/R group （anoxia for 120 min, reoxygenation for 240 min） and L-carnitine treatment group, in which cells were exposed to 20 mg/L, 50 mg/L, 100 mg/L, 200 mg/L L-carnitine respectively at 2 h before anoxia. The superoxide dismutase (SOD), succinate dehydrogenase (SDH) activities and malondialdehyde (MDA) content were examined, and the apoptosis was determined by flow of cytometry (FCM). In addition, the ultrastructure was observed by transmission electron microscopy. RESULTS: In A/R group, SOD and SDH activities were lower, the apoptosis rate and MDA content were higher than those in control group (P<0.05). In L-carnitine treatment group, SOD and SDH activities were higher, the apoptosis rate and MDA content were lower than those in A/R group, a L-carnitine concentration-dependent effect was found. Moreover, impairment of myocardial ultrastructure was more severe in A/R group than L-carnitine treatment group. CONCLUSION: L-carnitine can protect cardiomyocytes against hypoxia/reoxygenation-induced injury in a dose-dependent manner.     

Effects of NK cells on the etiology of severe pre-eclampsia

AIM: To detect the effects of NK cells on the etiology of severe pre-eclampsia. METHODS: The peripheral blood and umbilical blood from severe pre-eclamptic patients and normal late pregnant women were collected. NK cells were seperated in percoll and counted by SAP. The proliferating ability of NK cells was measured by MTT and killing power of NK cells was determined by ［51Cr］ releasing test. The immunohistochemistry method was used to detect the quantity of NK cells and the expression of HLA-G in placenta of them. RESULTS: (1) Compared with that in normal late pregnant women, the counts of the NK cells in peripheral blood, umbilical blood and decidua of severe pre-eclamptic patients were significantly higher (all P<0.05). (2) Compared with that in normal late pregnant women, proliferating and killing ability of NK cells in peripheral blood and umbilical blood of severe pre-eclamptic patients were significantly higher. (3) The expression of HLA-G protein in placenta of severe pre-eclampsia group was lower than that in normal group (P<0.05). CONCLUSION: The quantity of NK cells in severe pre-eclamptic patients is increased with stronger biologic function. They may be related with the pathogenesis of severe pre-eclampsia.     

昆虫病原线虫共生细菌胞内晶体蛋白的研究进展

初生型发光杆菌属（Photorhabdus）及嗜线虫致病杆菌属（Xenorhabdus）细菌分别与异小杆线虫属（Heterorhabdltis）和斯氏线虫属（Steinernema）昆虫病原线虫互惠共生。这类昆虫病原细菌在稳定生长期分别产生两种形态各异的胞内晶体蛋白。本文回顾了这类蛋白的研究历史和最新的研究进展，特别是胞内晶体蛋白的理化性质和生物学功能，同时讨论这种晶体蛋白的研究方法与技术。     

青岛市某猪场猪瘟的发生、诊断与防治报告

青岛某大型养猪场地处城阳区，饲养有杜洛克、长白等优良品种。饲养种母猪600多头，种公猪13头，全部采用人工授精配种。母猪平均每胎产仔数为9．75头。现存栏种公猪、母猪、仔猪、育成和育肥猪共有6500多头。全部采取自繁自养的方式。种母猪免疫过猪瘟、蓝耳病(PRRS)和伪狂犬病的二联灭活疫苗及细小病毒灭活疫苗。该场母猪免疫程序是：配种前一个月免疫4倍量猪瘟冻干苗，母猪产后在仔猪断奶前后进行猪瘟窝防(仔猪和母猪同时免疫)。     

Effects of antibiotic treatment on the inflammatory mediator expression in peritoneum and the peritoneal transport function in the rat with acute bacterial peritonitis

AIM: To observe the effect of antibiotic treatment on the inflammatory mediator expression in peritoneum and the peritoneal transport function in rats with acute peritonitis, and explore its mechanisms. METHODS: Eighty-six SD rats were randomly divided into three groups. Control group (n=28) were treated with PBS (ip), peritonitis group (n=28) and treatment group (n=28) were challenged with the E.coli (ip), but at 3 h and 9 h gentamicin was given (ip) in treatment group. Seven rats of every group were randomly sacrificed at 24 h, 48 h, 72 h and 7 d. Peritoneal equilibration test (PET) was did before they were killed. Leukocyte count, pathological changes and the expression of CD45, NF-κB， IL-1β， TNF-α in peritoneum were examined. RESULTS: (1)The blood leukocytes in peritonitis group decreased strikingly, but did not change obviously in other two groups. The peritoneal fluid leukocytes in peritonitis group increased significantly from 24 h to 72 h, while in treatment group, it enhanced more strikingly than peritonitis group at 24 h, and recovered earlier. (2) Both in peritonitis group and treatment group, the expression of activated NF-κB, IL-1β, TNF-α and CD45 increased significantly, but the treatment group was lower than model group at 48 h and 72 h. The mRNA level of IL-1β and TNF-α had the same trend as their protein expression. (3) Compared with the control group, UF and D/D0 Glu decreased significantly in model group and treatment group, and D/PTP increased dramatically. The D/P TP in treatment group lowered obviously compared with peritonitis group, while the net UF and D/D0 Glu had not significant difference between treatment group and model group. CONCLUSION: Antibiotic treatment can partly decrease the expression of inflammatory mediators in peritoneum of rats with acute peritonitis and also can improve the protein transport ability to some extent, but can not improve the peritoneal ultrafiltration and the glucose transport function.     

Intrathymic inoculation of liver specific antigen protects hepatocytes from apoptosis after liver allotransplantation

AIM: To study the effects of intrathymic inoculation of liver specific antigen (LSA) on hepatocyte apoptosis after liver allotransplantation. METHODS: Orthotopic liver transplantation was used in this study. Group Ⅰ: syngenic control (Wistar-to-Wistar); Group Ⅱ: acute rejection (SD-to-Wistar); Group Ⅲ: thymus inoculation of SD rat LSA day 7 before transplantation. The observation of general situation and survival time, hepatocyte apoptosis and LAT expression in liver transplants were used to analyze immune state of animals in different groups. RESULTS: The general situation of group Ⅰ was very well after transplantation. Recipients of groupⅡ lost body weight progressively and all died within day 9 to day 13 post transplantation. As for group Ⅲ, the general situation of recipients was remarkably better than that in group Ⅱ. The positive cells of apoptosis in group Ⅲ detected by TUNEL were not significantly different from that in group Ⅰ, but was significantly lower than that in group Ⅱ. LAT was detected at any time in group Ⅱ with peak expression at day 5 and day 7 post transplantation. In contrast, LAT was not detected in any other groups. CONCLUSION: Intrathymic inoculation of LSA protects hepatocytes from apoptosis after liver allotransplantation.     

应用RT-PCR方法检测水稻植株和介体昆虫体内的水稻齿叶矮缩病毒

 将生物学接种和RT-PCR检测水稻植株体内的水稻齿叶矮缩病毒的方法进行了比较,发现结果基本趋于一致,但总体上RT-PCR检测阳性率稍高于生物学接种的发病率。将生物学接种检测褐飞虱介体内水稻齿叶矮缩病毒方法与RT-PCR检测方法进行比较分析,发现RT-PCR方法可以检测单头褐飞虱体内的水稻齿叶矮缩病毒,具有很高的准确性和灵敏度。     

植酸酶对0～3周樱桃谷鸭生长性能、钙、磷表观存留率影响

为了在负对照组 (钙、磷水平各减少 0 .1 % )日粮基础上添加植酸酶对肉鸭生长、钙、磷表观存留率的影响 ,用 64只 1日龄樱桃谷鸭进行试验 ,按体重随机分为 4组 ,代谢期 6天。试验组鸭分别添加植酸酶 30 0和 50 0PU/kg。结果表明 :添加植酸酶显著提高试鸭的日采食量、日增重、钙、磷的表观存留率 ,植酸酶可以部分替代磷酸氢钙