Persistent detection of peste de petits ruminants antigen in the faeces of recovered goats

Peste des petits ruminants (PPR) disease was confirmed in West African Dwarf goats. They were managed symptomatically with antibiotics and antidarrhoeics. Following clinical recovery, faeces were collected every week from 40 recovered goats to monitor excretion of the PPR virus haemagglutinins in their faeces. All the 40 recovered goats shed the PPR virus haemagglutinins for 11 weeks post recovery. Nine goats (22.5%) continued shedding the viral antigen 12 weeks post recovery. There was correlation between weekly mean haemagglutination titre of the PPR virus and time post recovery with r = -0.7504 (p < 0.01).     

Seroprevalence of egg drop syndrome - 76 virus as cause of poor egg productivity of poultry in Nsukka,South East Nigeria

To determine if egg drop syndrome 76 virus infection is among the causes of lowered egg productivity in commercial poultry farms in South Eastern Part of Nigeria and to know the prevalence of the infection, ten farms with history of lowered egg production in Nsukka local government area of Enugu State were randomly selected. Sera from ten hens in each of the selected farms were assayed for antibodies against EDS 76 virus by the haemagglutination-inhibition (HI) test. The mean HI titre of the ten hens in each of the farms was recorded as EDS - 76 antibody titre for the farm. Nine out of the 10 farms tested were positive for EDS - 76 antibodies with HI titres ranging between 16 and 256. Out of 10 flocks with production of 65% and above 9 were EDS-76 HI negative.     

Bilateral Leydig cell tumor in a six-year-old intersex goat affected by Polled Intersex Syndrome

A 6-year-old, sterile, Blanca Celtibérica breed adult doe was referred to our faculty. The doe had external female genitalia, a short anogenital distance, and normally shaped udders. Masculinization signs in the head shape and male behavior were also noted at the time of referral. Genetic analysis demonstrated normal 2n = 60 XX karyotype and an absence of the sex-determining region Y (SRY). The animal was homozygous for a DNA deletion responsible for the Polled Intersex Syndrome (PIS). A uterus and 2 uterine horns were present at the postmortem examination. Gartner's ducts and degenerated Wolffian derivatives persisted. There were 2 intra-abdominal testicle-like structures, one of which consisted of epididymal and deferent ducts. An advanced Leydig cell tumor, resulting in almost total destruction of the intratesticular structures, was also observed. Leydig cell tumors usually produce testosterone. Thus, these histologic findings are compatible with the evident virilization.     

Effects of sowing time on bolting and returning green in welsh onion

Qiyechangbai and Hadacong1 were used to study the relationship of the leaf age of overwinter to bolting and returning green by the difference of sowing time.The results showed that the earlier the seeds were sown,the older leaf age of overwinter was,the higher the rates of returning green and bolting rate were,the earlier the bolting time was.The leaf age of overwinter of Qiyechangbai sown August 31 to September 14 was 2.1-3.1 leaves and the rate of returning green was 86.5%-92.1%,while the leaf age of overwinter of Hadacong1 sown September 7 to September 14 was 2.3-2.7 leaves and the rate of returning green was 88.5%-93.8%,both varieties didn't bolt.In addition,in the same sowing time,the bolting rate of Hadacong1 was higher than that of Qiyechangbai slightly.     

EFFICIENT CONTAMINANT REMOVAL FROM LIQUID DIGESTATE OF PIG MANURE BY CHEMICAL PRECIPITATION AND CO2 MINERALIZATION USING ALKALINE ASH

Chemical precipitation is a widely applied approach for a liquid fraction of digestate(LFD) of agricultural waste but its large-scale application requires low-cost and efficient precipitating agents and novel process design. This study evaluated novel approach for the efficient removal of contaminants from the LFD using fly ash-based chemical precipitation, followed by filtration and CO₂ mineralization. The technical feasibility of this approach was evaluated using p H and electrical ...     

Unintended Changes in Genetically Modified Rice Expressing the Lysine-Rich Fusion Protein Gene Revealed by a Proteomics Approach

Development of new technologies for evaluating genetically modified(GM) crops has revealed that there are unintended insertions and expression changes in GM crops.Profiling techniques are non-targeted approaches and are capable of detecting more unintended changes in GM crops.Here,we report the application of a comparative proteomic approach to investigate the protein profile differences between a GM rice line,which has a lysine-rich protein gene,and its non-transgenic parental line.Proteome analysis by two-dimensional gel electrophoresis(2-DE)and mass spectrum analysis of the seeds identified 22differentially expressed protein spots.Apart from a number of glutelins that were detected as targeted proteins in the GM line,the majority of the other changed proteins were involved in carbohydrate metabolism,protein synthesis and stress responses.These results indicated that the altered proteins were not associated with plant allergens or toxicity.     

燃料油与燃煤综合应用效益对比分析

对比了燃煤与燃油的燃烧值与其当前的市场价格,以及运煤车辆的耗油和大修的相关标准参数。从燃油与燃煤产生的能耗与需要投入的风险、运输、折旧及培训等费用成本两方面进行了综合分析。对此分析结果表明,当运煤距离超过300km时,燃油的综合效益高于燃煤,并有利于环保,避免资源重复利用,有效提高了资源的利用效益。