基因芯片法鉴别饲料中猪、牛、鸡和鸭等动物源性成分分析

研究了基因芯片技术在动物源性成分检测中的应用,在研究过程中选择mtDNA 18S rRNA为目标基因,在通用引物扩增区间设计了质控探针、特异性探针。最终验证了基因芯片检测技术在动物源性成分检测中的应用效果。本次研究证实了基因芯片检测技术在特异性、灵敏度等方面可以达到较高检测水平,该方法成本较低,灵敏度和准确性较高,可用于高通量检测饲料中是否含有猪、牛、鸡和鸭等4种动物源性成分。     

新形势下生猪生产发展的思考

生猪生产滑坡已发生一段时间了,猪肉价格上涨仍是社会关注的热点话题。近年来,随着农村二、三产业发展,生猪产值在经济总量中的占比和养猪收入在农民家庭收入中的占比开始减少。新形势下如何发展生猪生产,要消除认识上的六个误区。     

豇豆新品种浙翠9号的选育

浙翠9号是以浙翠2号为母本、夏宝2号为父本育成的长豇豆新品种。该品种中熟偏晚,生长势较强,主、侧蔓均可结荚,荚长69.5 cm、宽0.9 cm,单荚质量30.5 g,豆荚粗细匀称、肉厚、肉质致密;嫩荚浅绿色,商品性好;春季栽培全生育期为115~122 d,秋季栽培全生育期为105~112 d,抗逆性好,抗病毒病,中抗煤霉病、枯萎病。     

Toxic pneumonitis caused by inhalation of hydrocarbon waterproofing spray in two dogs

CASE DESCRIPTION: 2 dogs were evaluated because of vomiting and lethargy (a Toy Poodle; dog 1) and acute respiratory distress, vomiting, and anorexia (a Chihuahua; dog 2). Dog 1 had been exposed to a commercial hydrocarbon waterproofing spray 24 hours before the development of clinical signs, and dog 2 was examined 18 hours after exposure to a waterproofing spray containing heptane, a highly flammable liquid hydrocarbon. CLINICAL FINDINGS: In both dogs, major gastrointestinal tract abnormalities were ruled out but respiratory status worsened. Thoracic radiography revealed a diffuse interstitial pulmonary pattern, and hypoxemia was detected. TREATMENT AND OUTCOME: Hospitalization for monitoring and care was required for both dogs. The dogs recovered with supportive care, which included administration of oxygen, fluids, and bronchodilators. Additionally, dog 1 received glucocorticoids via inhalation and supplemental enteral nutrition, whereas dog 2 was treated with an antimicrobial. CLINICAL RELEVANCE: The dogs of this report developed hydrocarbon pneumonitis following exposure to waterproofing sprays. Such sprays contain potentially toxic hydrocarbons. The severity of the adverse effects associated with exposure may have been amplified because the dogs were physically small and were exposed to a relatively large amount of aerosolized spray within small areas. Development of chemical pneumonitis in pet animals is best prevented by application of waterproofing sprays in well-ventilated or outdoor areas from which pets have been excluded. With prolonged hospitalization and considerable monitoring and care, affected dogs can recover from these exposures.     

Responses of dogs to dietary omega-3 fatty acids

Long-chain omega-3 fatty acids from fish oil and other marine sources appear to be capable of modifying inflammatory and immune responses in dogs. Information is provided on the capacity of dogs to metabolize omega-3 fatty acids and the effects of omega-3 fatty acids on skin and coat, inflammatory responses, and neurologic development in puppies.     

AAHA/AAFP pain management guidelines for dogs & cats

Pain management in dogs and cats has undergone a dramatic evolution in the past decade. Current approaches focus on anticipation and prevention of pain, as well as both pharmacologic and nonpharmacologic management techniques. The veterinary team plays an essential role in educating pet owners about recognizing and managing pain in their pets.     

Glutathione content of in vivo and in vitro matured canine oocytes collected from different reproductive stages

Glutathione (GSH) concentrations of oocytes are considered as an important marker of the cytoplasmic maturation. The present study was designed to compare GSH concentrations of in vivo and in vitro matured canine oocytes. In vivo matured oocytes were collected 72 hr after ovulation by flushing fallopian tubes after laparotomy. Ovaries were collected from bitches with different reproductive stages, and collected oocytes were divided into 2 groups according to the size viz. < 120 microm and > 120 microm in diameter and cultured for 72 hr in Tissue Culture Medium-199 supplemented with 10% FBS, 2.2 mg/ml sodium bicarbonate, 2.0 microg/ml estrogen, 0.5 microg/ml FSH, 0.03 IU/ml hCG, and 1% penicillin-streptomycin solution in the presence or absence of 50 microM beta-mercaptoethanol. GSH concentrations were determined by the dithionitrobenzoic acid-glutathione disulfide (DTNB-GSSG) reductase recycling assay. GSH concentrations of immature canine oocytes were 2.9 and 3.8, 3.5 and 6.8, and 3.1 and 6.5 pM/oocyte for < 120 microm and > 120 microm in diameter oocyte groups at anestrous, follicular and luteal stage, respectively (P<0.05). In vivo matured oocytes had significantly higher GSH concentrations compared with in vitro matured oocytes. The GSH content was 19.2 pM/oocyte for in vivo matured oocytes, while 4.1 to 8.1 and 5.7 to 13.2 pM/oocyte for in vitro matured oocytes cultured in the absence or presence of beta-mercaptoethanol, respectively (P<0.05). Presence of beta-mercaptoethanol increased GSH synthesis in canine oocytes cultured in vitro, and oocytes collected from follicular and luteal stage was superior to anestrus oocytes.